TY  - JOUR
AU  - Kekić, Dusan
AU  - Gajić, Ina
AU  - Opavski, Nataša
AU  - Kojić, Milan
AU  - Vukotić, Goran
AU  - Smitran, Aleksandra
AU  - Bosković, Lidija
AU  - Stojković, Marina
AU  - Ranin, Lazar
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1493
AB  - Group B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal disease rate, to reveal the trends of antimicrobial resistance and serotype distribution of GBS from various patient groups. Randomly selected non-invasive (n=991) and all invasive GBS (n=80) collected throughout Serbia from 2015 to 2020 were tested for antimicrobial susceptibility, capsular typing, and hvgA detection. Overall, 877/5621 (15.6%) pregnant women were colonised with GBS. Invasive GBS infections incidence in infants (0.18/1000 live births) showed a decreasing trend (0.3 to 0.1/1000 live births). Type III was overrepresented in infants with invasive infections (n=35, 58.3%), whereas type V predominated among colonised adults (n=224, 25.5%) and those with noninvasive (n=37, 32.5%) and invasive infections (n=8, 40%). The hypervirulent clone III/ST17 was highly associated with invasive infections (n=28, 35%), particularly late-onset disease (n=9, 47.4%), showing an increase from 12.3 to 14.8%. The GBS resistance to erythromycin and clindamycin was 26.7% and 22.1%, respectively, with an upward trend. The emergence of the hypervirulent clone III/ST17 and the escalation in GBS resistance highlight an urgent need for continuous monitoring of GBS infections.
PB  - Nature Research, Berlin
T2  - Scientific Reports
T1  - Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020
IS  - 1
VL  - 11
DO  - 10.1038/s41598-020-79354-3
ER  - 

@article{
author = "Kekić, Dusan and Gajić, Ina and Opavski, Nataša and Kojić, Milan and Vukotić, Goran and Smitran, Aleksandra and Bosković, Lidija and Stojković, Marina and Ranin, Lazar",
year = "2021",
abstract = "Group B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal disease rate, to reveal the trends of antimicrobial resistance and serotype distribution of GBS from various patient groups. Randomly selected non-invasive (n=991) and all invasive GBS (n=80) collected throughout Serbia from 2015 to 2020 were tested for antimicrobial susceptibility, capsular typing, and hvgA detection. Overall, 877/5621 (15.6%) pregnant women were colonised with GBS. Invasive GBS infections incidence in infants (0.18/1000 live births) showed a decreasing trend (0.3 to 0.1/1000 live births). Type III was overrepresented in infants with invasive infections (n=35, 58.3%), whereas type V predominated among colonised adults (n=224, 25.5%) and those with noninvasive (n=37, 32.5%) and invasive infections (n=8, 40%). The hypervirulent clone III/ST17 was highly associated with invasive infections (n=28, 35%), particularly late-onset disease (n=9, 47.4%), showing an increase from 12.3 to 14.8%. The GBS resistance to erythromycin and clindamycin was 26.7% and 22.1%, respectively, with an upward trend. The emergence of the hypervirulent clone III/ST17 and the escalation in GBS resistance highlight an urgent need for continuous monitoring of GBS infections.",
publisher = "Nature Research, Berlin",
journal = "Scientific Reports",
title = "Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020",
number = "1",
volume = "11",
doi = "10.1038/s41598-020-79354-3"
}

Kekić, D., Gajić, I., Opavski, N., Kojić, M., Vukotić, G., Smitran, A., Bosković, L., Stojković, M.,& Ranin, L.. (2021). Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020. in Scientific Reports
Nature Research, Berlin., 11(1).
https://doi.org/10.1038/s41598-020-79354-3

Kekić D, Gajić I, Opavski N, Kojić M, Vukotić G, Smitran A, Bosković L, Stojković M, Ranin L. Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-020-79354-3 .

Kekić, Dusan, Gajić, Ina, Opavski, Nataša, Kojić, Milan, Vukotić, Goran, Smitran, Aleksandra, Bosković, Lidija, Stojković, Marina, Ranin, Lazar, "Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-020-79354-3 . .

TY  - JOUR
AU  - Luković, Bojana
AU  - Gajić, Ina
AU  - Dimkić, Ivica
AU  - Kekić, Dusan
AU  - Zornić, Sanja
AU  - Pozder, Tatjana
AU  - Radisavljević, Svetlana
AU  - Opavski, Nataša
AU  - Kojić, Milan
AU  - Ranin, Lazar
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1309
AB  - Background The worldwide emergence and clonal spread of carbapenem-resistantAcinetobacter baumannii(CRAB) is of great concern. The aim of this nationwide study was to investigate the prevalence of CRAB isolates in Serbia and to characterize underlying resistance mechanisms and their genetic relatedness. Methods Non-redundant clinical samples obtained from hospitalized patients throughout Serbia were included in the prospective, observational, multicenter study conducted from January to June 2018. Samples were initially screened for the presence ofAcinetobacter baumannii-calcoaceticus(Acb) complex using conventional bacteriological techniques. Acb complexes recovered from clinical samples obtained from inpatients with confirmed bacterial infections were further evaluated for the presence ofA. baumannii. Identification to the species level was done by the detection of thebla(OXA-51)gene andrpoBgene sequence analysis. Susceptibility testing was done by disk diffusion and broth microdilution method. CRAB isolates were tested for the presence of acquired carbapenemases(bla(OXA-24-like),bla(OXA-23-like,)bla(OXA-58-like),bla(OXA-143-like),bla(IMP),bla(VIM),bla(GIM),bla(SPM),bla(SIM),bla(NDM)) by PCR. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results Acb complex was isolated in 280 out of 2401 clinical samples (11.6%). Overall,A. baumanniiwas identified in 237 out of 280 Acb complex (84.6%). CRAB prevalence was found to be 93.7% (237/222). The MIC50/MIC(90)for imipenem and meropenem were 8/ gt  32 mu g/mL and 16/ gt  32 mu g/mL, respectively. Although susceptibility was high for colistin (95.7%;n = 227) and tigecycline (75.1%;n = 178), ten isolates (4.3%) were classified as pandrug-resistant. The following carbapenemases-encoding genes were found: 98 (44.2%)bla(OXA-24-like), 76 (34.5%)bla(OXA-23-like), and 7 (3.2%)bla(NDM-1). PFGE analysis revealed six different clusters. MLST analysis identified three STs: ST2 (n = 13), ST492 (n = 14), and ST636 (n = 10). Obtained results evaluated that circulating CRAB clones in Serbia were as follows:bla(OXA66)/bla(OXA23)/ST2 (32.4%),bla(OXA66)/bla(OXA23)/bla(OXA72)/ST2 (2.7%),bla(OXA66)/bla(OXA72)/ST492 (37.8%), andbla(OXA66)/bla(OXA72)/ST636 (27.1%). Conclusion This study revealed extremely high proportions of carbapenem resistance amongA. baumanniiclinical isolates due to the emergence ofbla(OXA-72),bla(OXA-23), andbla(NDM-1)genes among CRAB isolates in Serbia and their clonal propagation.
PB  - BMC, London
T2  - Antimicrobial Resistance and Infection Control
T1  - The first nationwide multicenter study ofAcinetobacter baumanniirecovered in Serbia: emergence of OXA-72, OXA-23 and NDM-1-producing isolates
IS  - 1
VL  - 9
DO  - 10.1186/s13756-020-00769-8
ER  - 

@article{
author = "Luković, Bojana and Gajić, Ina and Dimkić, Ivica and Kekić, Dusan and Zornić, Sanja and Pozder, Tatjana and Radisavljević, Svetlana and Opavski, Nataša and Kojić, Milan and Ranin, Lazar",
year = "2020",
abstract = "Background The worldwide emergence and clonal spread of carbapenem-resistantAcinetobacter baumannii(CRAB) is of great concern. The aim of this nationwide study was to investigate the prevalence of CRAB isolates in Serbia and to characterize underlying resistance mechanisms and their genetic relatedness. Methods Non-redundant clinical samples obtained from hospitalized patients throughout Serbia were included in the prospective, observational, multicenter study conducted from January to June 2018. Samples were initially screened for the presence ofAcinetobacter baumannii-calcoaceticus(Acb) complex using conventional bacteriological techniques. Acb complexes recovered from clinical samples obtained from inpatients with confirmed bacterial infections were further evaluated for the presence ofA. baumannii. Identification to the species level was done by the detection of thebla(OXA-51)gene andrpoBgene sequence analysis. Susceptibility testing was done by disk diffusion and broth microdilution method. CRAB isolates were tested for the presence of acquired carbapenemases(bla(OXA-24-like),bla(OXA-23-like,)bla(OXA-58-like),bla(OXA-143-like),bla(IMP),bla(VIM),bla(GIM),bla(SPM),bla(SIM),bla(NDM)) by PCR. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results Acb complex was isolated in 280 out of 2401 clinical samples (11.6%). Overall,A. baumanniiwas identified in 237 out of 280 Acb complex (84.6%). CRAB prevalence was found to be 93.7% (237/222). The MIC50/MIC(90)for imipenem and meropenem were 8/ gt  32 mu g/mL and 16/ gt  32 mu g/mL, respectively. Although susceptibility was high for colistin (95.7%;n = 227) and tigecycline (75.1%;n = 178), ten isolates (4.3%) were classified as pandrug-resistant. The following carbapenemases-encoding genes were found: 98 (44.2%)bla(OXA-24-like), 76 (34.5%)bla(OXA-23-like), and 7 (3.2%)bla(NDM-1). PFGE analysis revealed six different clusters. MLST analysis identified three STs: ST2 (n = 13), ST492 (n = 14), and ST636 (n = 10). Obtained results evaluated that circulating CRAB clones in Serbia were as follows:bla(OXA66)/bla(OXA23)/ST2 (32.4%),bla(OXA66)/bla(OXA23)/bla(OXA72)/ST2 (2.7%),bla(OXA66)/bla(OXA72)/ST492 (37.8%), andbla(OXA66)/bla(OXA72)/ST636 (27.1%). Conclusion This study revealed extremely high proportions of carbapenem resistance amongA. baumanniiclinical isolates due to the emergence ofbla(OXA-72),bla(OXA-23), andbla(NDM-1)genes among CRAB isolates in Serbia and their clonal propagation.",
publisher = "BMC, London",
journal = "Antimicrobial Resistance and Infection Control",
title = "The first nationwide multicenter study ofAcinetobacter baumanniirecovered in Serbia: emergence of OXA-72, OXA-23 and NDM-1-producing isolates",
number = "1",
volume = "9",
doi = "10.1186/s13756-020-00769-8"
}

Luković, B., Gajić, I., Dimkić, I., Kekić, D., Zornić, S., Pozder, T., Radisavljević, S., Opavski, N., Kojić, M.,& Ranin, L.. (2020). The first nationwide multicenter study ofAcinetobacter baumanniirecovered in Serbia: emergence of OXA-72, OXA-23 and NDM-1-producing isolates. in Antimicrobial Resistance and Infection Control
BMC, London., 9(1).
https://doi.org/10.1186/s13756-020-00769-8

Luković B, Gajić I, Dimkić I, Kekić D, Zornić S, Pozder T, Radisavljević S, Opavski N, Kojić M, Ranin L. The first nationwide multicenter study ofAcinetobacter baumanniirecovered in Serbia: emergence of OXA-72, OXA-23 and NDM-1-producing isolates. in Antimicrobial Resistance and Infection Control. 2020;9(1).
doi:10.1186/s13756-020-00769-8 .

Luković, Bojana, Gajić, Ina, Dimkić, Ivica, Kekić, Dusan, Zornić, Sanja, Pozder, Tatjana, Radisavljević, Svetlana, Opavski, Nataša, Kojić, Milan, Ranin, Lazar, "The first nationwide multicenter study ofAcinetobacter baumanniirecovered in Serbia: emergence of OXA-72, OXA-23 and NDM-1-producing isolates" in Antimicrobial Resistance and Infection Control, 9, no. 1 (2020),
https://doi.org/10.1186/s13756-020-00769-8 . .

TY  - JOUR
AU  - Cirković, Ivana
AU  - Bozić, Dragana D.
AU  - Draganić, Veselin
AU  - Lozo, Jelena
AU  - Berić, Tanja
AU  - Kojić, Milan
AU  - Arsić, Biljana
AU  - Garalejić, Eliana
AU  - Đukić, Slobodanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/958
AB  - Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates
IS  - 12
VL  - 11
DO  - 10.1371/journal.pone.0167995
ER  - 

@article{
author = "Cirković, Ivana and Bozić, Dragana D. and Draganić, Veselin and Lozo, Jelena and Berić, Tanja and Kojić, Milan and Arsić, Biljana and Garalejić, Eliana and Đukić, Slobodanka and Stanković, Slaviša",
year = "2016",
abstract = "Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates",
number = "12",
volume = "11",
doi = "10.1371/journal.pone.0167995"
}

Cirković, I., Bozić, D. D., Draganić, V., Lozo, J., Berić, T., Kojić, M., Arsić, B., Garalejić, E., Đukić, S.,& Stanković, S.. (2016). Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167995

Cirković I, Bozić DD, Draganić V, Lozo J, Berić T, Kojić M, Arsić B, Garalejić E, Đukić S, Stanković S. Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One. 2016;11(12).
doi:10.1371/journal.pone.0167995 .

Cirković, Ivana, Bozić, Dragana D., Draganić, Veselin, Lozo, Jelena, Berić, Tanja, Kojić, Milan, Arsić, Biljana, Garalejić, Eliana, Đukić, Slobodanka, Stanković, Slaviša, "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates" in PLoS One, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167995 . .