TY  - JOUR
AU  - Zavišić, Gordana
AU  - Radulović, Željka
AU  - Vranić, Valentina
AU  - Begović, Jelena
AU  - Topisirović, Ljubiša
AU  - Strahinić, Ivana
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/538
AB  - The aim of this study was to investigate the probiotic potential of bacteriocin-producing lactobacilli strain Lactobacillus plantarum G2 isolated from the vaginal mucus of healthy women. The antimicrobial effect of G2 was confirmed in the mixed culture with pathogenic Escherichia coli, Staphylococcus aureus, Salmonella abony and Pseudomonas aeruginosa, while bacteriocine activity was detected against S. aureus and S. abony only. The strain showed an excellent survival rate in low pH and in the presence of bile salts. The percentage of adhered cells of L. plantarum G2 to hexadecane was 63.85±2.0 indicating the intermediate hydrophobicity.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Characterization and antimicrobial activity of vaginal lactobacillus isolate
EP  - 35
IS  - 1
SP  - 29
VL  - 63
DO  - 10.2298/ABS1101029Z
ER  - 

@article{
author = "Zavišić, Gordana and Radulović, Željka and Vranić, Valentina and Begović, Jelena and Topisirović, Ljubiša and Strahinić, Ivana",
year = "2011",
abstract = "The aim of this study was to investigate the probiotic potential of bacteriocin-producing lactobacilli strain Lactobacillus plantarum G2 isolated from the vaginal mucus of healthy women. The antimicrobial effect of G2 was confirmed in the mixed culture with pathogenic Escherichia coli, Staphylococcus aureus, Salmonella abony and Pseudomonas aeruginosa, while bacteriocine activity was detected against S. aureus and S. abony only. The strain showed an excellent survival rate in low pH and in the presence of bile salts. The percentage of adhered cells of L. plantarum G2 to hexadecane was 63.85±2.0 indicating the intermediate hydrophobicity.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Characterization and antimicrobial activity of vaginal lactobacillus isolate",
pages = "35-29",
number = "1",
volume = "63",
doi = "10.2298/ABS1101029Z"
}

Zavišić, G., Radulović, Ž., Vranić, V., Begović, J., Topisirović, L.,& Strahinić, I.. (2011). Characterization and antimicrobial activity of vaginal lactobacillus isolate. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(1), 29-35.
https://doi.org/10.2298/ABS1101029Z

Zavišić G, Radulović Ž, Vranić V, Begović J, Topisirović L, Strahinić I. Characterization and antimicrobial activity of vaginal lactobacillus isolate. in Archives of Biological Sciences. 2011;63(1):29-35.
doi:10.2298/ABS1101029Z .

Zavišić, Gordana, Radulović, Željka, Vranić, Valentina, Begović, Jelena, Topisirović, Ljubiša, Strahinić, Ivana, "Characterization and antimicrobial activity of vaginal lactobacillus isolate" in Archives of Biological Sciences, 63, no. 1 (2011):29-35,
https://doi.org/10.2298/ABS1101029Z . .

TY  - JOUR
AU  - Begović, Jelena
AU  - Brandsma, J.B.
AU  - Jovčić, Branko
AU  - Tolinački, Maja
AU  - Veljović, Katarina
AU  - Meijer, W.C.
AU  - Topisirović, Ljubiša
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/536
AB  - Traditional Serbian cheese production has a long history and generates products with rich flavor profiles. To enable the industrial manufacture of these home-made Serbian cheeses, the lactic acid bacteria present in them needs to be characterized. Five fresh white cheeses made from raw cow's milk without commercial starter cultures were collected from households on the mountain Stara Planina, Serbia. According to phenotypical and molecular analysis, 262 isolated LAB were found to belong to Lactococcus, Lactobacillus, Streptococcus, Leuconostoc or Enterococcus. The unique bacterial composition of each cheese indicates that the preservation of household industry is the way to maintain production of distinct cheeses.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Analysis of dominant lactic acid bacteria from artisanal raw milk cheeses produced on the mountain Stara Planina, Serbia
EP  - 20
IS  - 1
SP  - 11
VL  - 63
DO  - 10.2298/ABS1101011B
ER  - 

@article{
author = "Begović, Jelena and Brandsma, J.B. and Jovčić, Branko and Tolinački, Maja and Veljović, Katarina and Meijer, W.C. and Topisirović, Ljubiša",
year = "2011",
abstract = "Traditional Serbian cheese production has a long history and generates products with rich flavor profiles. To enable the industrial manufacture of these home-made Serbian cheeses, the lactic acid bacteria present in them needs to be characterized. Five fresh white cheeses made from raw cow's milk without commercial starter cultures were collected from households on the mountain Stara Planina, Serbia. According to phenotypical and molecular analysis, 262 isolated LAB were found to belong to Lactococcus, Lactobacillus, Streptococcus, Leuconostoc or Enterococcus. The unique bacterial composition of each cheese indicates that the preservation of household industry is the way to maintain production of distinct cheeses.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Analysis of dominant lactic acid bacteria from artisanal raw milk cheeses produced on the mountain Stara Planina, Serbia",
pages = "20-11",
number = "1",
volume = "63",
doi = "10.2298/ABS1101011B"
}

Begović, J., Brandsma, J.B., Jovčić, B., Tolinački, M., Veljović, K., Meijer, W.C.,& Topisirović, L.. (2011). Analysis of dominant lactic acid bacteria from artisanal raw milk cheeses produced on the mountain Stara Planina, Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(1), 11-20.
https://doi.org/10.2298/ABS1101011B

Begović J, Brandsma J, Jovčić B, Tolinački M, Veljović K, Meijer W, Topisirović L. Analysis of dominant lactic acid bacteria from artisanal raw milk cheeses produced on the mountain Stara Planina, Serbia. in Archives of Biological Sciences. 2011;63(1):11-20.
doi:10.2298/ABS1101011B .

Begović, Jelena, Brandsma, J.B., Jovčić, Branko, Tolinački, Maja, Veljović, Katarina, Meijer, W.C., Topisirović, Ljubiša, "Analysis of dominant lactic acid bacteria from artisanal raw milk cheeses produced on the mountain Stara Planina, Serbia" in Archives of Biological Sciences, 63, no. 1 (2011):11-20,
https://doi.org/10.2298/ABS1101011B . .

TY  - JOUR
AU  - Joković, Nataša
AU  - Vukasinović, Maja
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Topisirović, Ljubiša
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/535
AB  - Two hundred thirteen non-starter lactic acid bacteria isolated from Radan cheese during ripening were identified with both a classical biochemical test and rep-PCR with (GTG)5 primer. For most isolates, which belong to the Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides, Lactobacillus plantarum, Lactobacillus paraplantarum and Enterococcus faecium, a phenotypic identification was in good agreement with rep-PCR identification. Lactococeus lactis subsp. lactis, Enterococcus faecium and subspecies from the Lenconostoc mesenteroides group were the dominant population of lactic acid bacteria in cheese until 10 days of ripening and only one Streptococcus thermophilus strain was isolated from the 5-day-old cheese sample. As ripening progressed, Lactobacillus plantarum became the predominant species together with the group of heterofermentative species of lactobacilli that could not be precisely identified with rep-PCR.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Characterization of non-starter lactic acid bacteria in traditionally produced home-made Radan cheese during ripening
EP  - 10
IS  - 1
SP  - 1
VL  - 63
DO  - 10.2298/ABS1101001J
ER  - 

@article{
author = "Joković, Nataša and Vukasinović, Maja and Veljović, Katarina and Tolinački, Maja and Topisirović, Ljubiša",
year = "2011",
abstract = "Two hundred thirteen non-starter lactic acid bacteria isolated from Radan cheese during ripening were identified with both a classical biochemical test and rep-PCR with (GTG)5 primer. For most isolates, which belong to the Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides, Lactobacillus plantarum, Lactobacillus paraplantarum and Enterococcus faecium, a phenotypic identification was in good agreement with rep-PCR identification. Lactococeus lactis subsp. lactis, Enterococcus faecium and subspecies from the Lenconostoc mesenteroides group were the dominant population of lactic acid bacteria in cheese until 10 days of ripening and only one Streptococcus thermophilus strain was isolated from the 5-day-old cheese sample. As ripening progressed, Lactobacillus plantarum became the predominant species together with the group of heterofermentative species of lactobacilli that could not be precisely identified with rep-PCR.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Characterization of non-starter lactic acid bacteria in traditionally produced home-made Radan cheese during ripening",
pages = "10-1",
number = "1",
volume = "63",
doi = "10.2298/ABS1101001J"
}

Joković, N., Vukasinović, M., Veljović, K., Tolinački, M.,& Topisirović, L.. (2011). Characterization of non-starter lactic acid bacteria in traditionally produced home-made Radan cheese during ripening. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(1), 1-10.
https://doi.org/10.2298/ABS1101001J

Joković N, Vukasinović M, Veljović K, Tolinački M, Topisirović L. Characterization of non-starter lactic acid bacteria in traditionally produced home-made Radan cheese during ripening. in Archives of Biological Sciences. 2011;63(1):1-10.
doi:10.2298/ABS1101001J .

Joković, Nataša, Vukasinović, Maja, Veljović, Katarina, Tolinački, Maja, Topisirović, Ljubiša, "Characterization of non-starter lactic acid bacteria in traditionally produced home-made Radan cheese during ripening" in Archives of Biological Sciences, 63, no. 1 (2011):1-10,
https://doi.org/10.2298/ABS1101001J . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Vasiljević, Zorica
AU  - Đukić, Slobodanka
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/541
AB  - Molecular detection and surveillance of the
resistance genes harboured by
Pseudomonas aeruginosa are becoming
increasingly important in assessing and
controlling spread and colonization in
hospitals, and in guiding the antibiotic
treatment of infections. Metallo-blactamase (MBL)-producing P. aeruginosa
strains are slowly but steadily increasing
within hospitals, causing outbreaks and/or
hyperendemic situations in some centres,
mostly in the Far East and the south of
Europe (Queenan & Bush, 2007). The
global dissemination of MBL-producing
P. aeruginosa strains has also reached the
Balkan region (Lepsanovic et al., 2008;
Sardelic et al., 2003). The objective of our
study was to detect and characterize P.
aeruginosa isolates producing MBLs from
the 400-bed paediatric tertiary care
hospital Mother and Child Health Institute
of Serbia ‘Dr Vukan Cupic’
PB  - Soc General Microbiology, Reading
T2  - Journal of Medical Microbiology
T1  - Emergence of VIM-2 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates in a paediatric hospital in Serbia
EP  - 869
IS  - 6
SP  - 868
VL  - 60
DO  - 10.1099/jmm.0.029173-0
ER  - 

@article{
author = "Jovčić, Branko and Vasiljević, Zorica and Đukić, Slobodanka and Topisirović, Ljubiša and Kojić, Milan",
year = "2011",
abstract = "Molecular detection and surveillance of the
resistance genes harboured by
Pseudomonas aeruginosa are becoming
increasingly important in assessing and
controlling spread and colonization in
hospitals, and in guiding the antibiotic
treatment of infections. Metallo-blactamase (MBL)-producing P. aeruginosa
strains are slowly but steadily increasing
within hospitals, causing outbreaks and/or
hyperendemic situations in some centres,
mostly in the Far East and the south of
Europe (Queenan & Bush, 2007). The
global dissemination of MBL-producing
P. aeruginosa strains has also reached the
Balkan region (Lepsanovic et al., 2008;
Sardelic et al., 2003). The objective of our
study was to detect and characterize P.
aeruginosa isolates producing MBLs from
the 400-bed paediatric tertiary care
hospital Mother and Child Health Institute
of Serbia ‘Dr Vukan Cupic’",
publisher = "Soc General Microbiology, Reading",
journal = "Journal of Medical Microbiology",
title = "Emergence of VIM-2 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates in a paediatric hospital in Serbia",
pages = "869-868",
number = "6",
volume = "60",
doi = "10.1099/jmm.0.029173-0"
}

Jovčić, B., Vasiljević, Z., Đukić, S., Topisirović, L.,& Kojić, M.. (2011). Emergence of VIM-2 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates in a paediatric hospital in Serbia. in Journal of Medical Microbiology
Soc General Microbiology, Reading., 60(6), 868-869.
https://doi.org/10.1099/jmm.0.029173-0

Jovčić B, Vasiljević Z, Đukić S, Topisirović L, Kojić M. Emergence of VIM-2 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates in a paediatric hospital in Serbia. in Journal of Medical Microbiology. 2011;60(6):868-869.
doi:10.1099/jmm.0.029173-0 .

Jovčić, Branko, Vasiljević, Zorica, Đukić, Slobodanka, Topisirović, Ljubiša, Kojić, Milan, "Emergence of VIM-2 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolates in a paediatric hospital in Serbia" in Journal of Medical Microbiology, 60, no. 6 (2011):868-869,
https://doi.org/10.1099/jmm.0.029173-0 . .

TY  - JOUR
AU  - Danilović, Bojana
AU  - Joković, Nataša
AU  - Petrović, Ljiljana
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Savić, D.
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/540
AB  - Petrovska Klobasa is an artisan Serbian sausage made only from meat and spices without any additives or starter cultures. In order to characterise lactic acid bacteria (LAB) microflora, a total number of 404 LAB strains were isolated from 15 samples collected during 90 days of the fermentation and 120 days of storage of one batch of Petrovska Klobasa. The isolates were preliminarily identified by phenotypic tests and subjected to (GTG)(5)-PCR fingerprinting. Representatives of each group were identified by 165 rDNA sequencing. The results showed that among the isolates, Lactobacillus sakei and Leuconostoc mesenteroides predominate with 36.4% and 37.1% of total LAB strains, respectively. Pediococcus pentosaceus was also isolated in high proportion (18.4%) whereas Enterococcus durans and Enterococcus caseliflavus made only 1% and 6% of the total isolates, correspondingly. The analysis of vacuum packed and modified atmosphere packed (MAP) samples showed higher presence of L mesenteroides and L sakei in the total microflora.
PB  - Elsevier Sci Ltd, Oxford
T2  - Meat Science
T1  - The characterisation of lactic acid bacteria during the fermentation of an artisan Serbian sausage (Petrovska Klobasa)
EP  - 674
IS  - 4
SP  - 668
VL  - 88
DO  - 10.1016/j.meatsci.2011.02.026
ER  - 

@article{
author = "Danilović, Bojana and Joković, Nataša and Petrović, Ljiljana and Veljović, Katarina and Tolinački, Maja and Savić, D.",
year = "2011",
abstract = "Petrovska Klobasa is an artisan Serbian sausage made only from meat and spices without any additives or starter cultures. In order to characterise lactic acid bacteria (LAB) microflora, a total number of 404 LAB strains were isolated from 15 samples collected during 90 days of the fermentation and 120 days of storage of one batch of Petrovska Klobasa. The isolates were preliminarily identified by phenotypic tests and subjected to (GTG)(5)-PCR fingerprinting. Representatives of each group were identified by 165 rDNA sequencing. The results showed that among the isolates, Lactobacillus sakei and Leuconostoc mesenteroides predominate with 36.4% and 37.1% of total LAB strains, respectively. Pediococcus pentosaceus was also isolated in high proportion (18.4%) whereas Enterococcus durans and Enterococcus caseliflavus made only 1% and 6% of the total isolates, correspondingly. The analysis of vacuum packed and modified atmosphere packed (MAP) samples showed higher presence of L mesenteroides and L sakei in the total microflora.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Meat Science",
title = "The characterisation of lactic acid bacteria during the fermentation of an artisan Serbian sausage (Petrovska Klobasa)",
pages = "674-668",
number = "4",
volume = "88",
doi = "10.1016/j.meatsci.2011.02.026"
}

Danilović, B., Joković, N., Petrović, L., Veljović, K., Tolinački, M.,& Savić, D.. (2011). The characterisation of lactic acid bacteria during the fermentation of an artisan Serbian sausage (Petrovska Klobasa). in Meat Science
Elsevier Sci Ltd, Oxford., 88(4), 668-674.
https://doi.org/10.1016/j.meatsci.2011.02.026

Danilović B, Joković N, Petrović L, Veljović K, Tolinački M, Savić D. The characterisation of lactic acid bacteria during the fermentation of an artisan Serbian sausage (Petrovska Klobasa). in Meat Science. 2011;88(4):668-674.
doi:10.1016/j.meatsci.2011.02.026 .

Danilović, Bojana, Joković, Nataša, Petrović, Ljiljana, Veljović, Katarina, Tolinački, Maja, Savić, D., "The characterisation of lactic acid bacteria during the fermentation of an artisan Serbian sausage (Petrovska Klobasa)" in Meat Science, 88, no. 4 (2011):668-674,
https://doi.org/10.1016/j.meatsci.2011.02.026 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Venturi, Vittorio
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/534
AB  - Pseudomonas sp. strain ATCC19151 is a natural isolate from sewage with the ability to degrade detergents. Genes encoding potential choline sulfatase (betC), substrate-binding ABC transporter protein (betD), sulfate transporter (betE), and divergent putative transcriptional regulator (betR) were cloned and characterized from strain ATCC19151. In silico analysis revealed that (1) the BetC protein belongs to alkPPc superfamily and shares CXPXR sequence with the cysteine sulfatases of group I, (2) BetR belongs to the LysR family of transcriptional regulators, (3) BetD is part of the PBPb superfamily of periplasmic and membrane-associated proteins, and (4) BetE is a permease and contains STAS domain. Insertional mutagenesis and genetic complementation show that betC gene encodes a functional choline sulfatase. Analysis of the betC (P (betC) ) and betR (P (betR) ) promoters revealed that they are inducible. BetR activates betC and betR transcription in the presence of choline sulfate, whilst in the absence of choline sulfate, BetR represses its own transcription. It was further established that BetR directly binds to betC-betR intergenic region in vitro, with higher affinity in the presence of choline sulfate as cofactor. Transcription of betC and betR was not induced in the presence of high concentration of NaCl.
PB  - Springer, New York
T2  - Archives of Microbiology
T1  - Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151
EP  - 405
IS  - 6
SP  - 399
VL  - 193
DO  - 10.1007/s00203-011-0685-x
ER  - 

@article{
author = "Jovčić, Branko and Venturi, Vittorio and Topisirović, Ljubiša and Kojić, Milan",
year = "2011",
abstract = "Pseudomonas sp. strain ATCC19151 is a natural isolate from sewage with the ability to degrade detergents. Genes encoding potential choline sulfatase (betC), substrate-binding ABC transporter protein (betD), sulfate transporter (betE), and divergent putative transcriptional regulator (betR) were cloned and characterized from strain ATCC19151. In silico analysis revealed that (1) the BetC protein belongs to alkPPc superfamily and shares CXPXR sequence with the cysteine sulfatases of group I, (2) BetR belongs to the LysR family of transcriptional regulators, (3) BetD is part of the PBPb superfamily of periplasmic and membrane-associated proteins, and (4) BetE is a permease and contains STAS domain. Insertional mutagenesis and genetic complementation show that betC gene encodes a functional choline sulfatase. Analysis of the betC (P (betC) ) and betR (P (betR) ) promoters revealed that they are inducible. BetR activates betC and betR transcription in the presence of choline sulfate, whilst in the absence of choline sulfate, BetR represses its own transcription. It was further established that BetR directly binds to betC-betR intergenic region in vitro, with higher affinity in the presence of choline sulfate as cofactor. Transcription of betC and betR was not induced in the presence of high concentration of NaCl.",
publisher = "Springer, New York",
journal = "Archives of Microbiology",
title = "Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151",
pages = "405-399",
number = "6",
volume = "193",
doi = "10.1007/s00203-011-0685-x"
}

Jovčić, B., Venturi, V., Topisirović, L.,& Kojić, M.. (2011). Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151. in Archives of Microbiology
Springer, New York., 193(6), 399-405.
https://doi.org/10.1007/s00203-011-0685-x

Jovčić B, Venturi V, Topisirović L, Kojić M. Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151. in Archives of Microbiology. 2011;193(6):399-405.
doi:10.1007/s00203-011-0685-x .

Jovčić, Branko, Venturi, Vittorio, Topisirović, Ljubiša, Kojić, Milan, "Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp ATCC19151" in Archives of Microbiology, 193, no. 6 (2011):399-405,
https://doi.org/10.1007/s00203-011-0685-x . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Venturi, V.
AU  - Davison, J.
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/452
AB  - Aims: The presented study was aimed to reveal transcriptional regulation of genes involved in SDS degradation (sdsA and sdsB) in Pseudomonas sp. ATCC19151. In addition, the ability of Pseudomonas sp. ATCC19151 to degrade anionic surfactants present in commercial detergent and septic tank drain was analysed. Methods and Results: Strain ATCC19151, at 30 degrees C, degrades all SDS present in the liquid medium (up to 4% w/v of SDS) within 48 h. ATCC19151 grows in the presence up to 15% (v/v) 'Fairy' commercial detergent and mineralizes 35% of present anionic surfactants. Analysis of the sdsA (P(sdsA)) and divergent sdsB (P(sdsB)) gene promoter activities revealed that SdsB acts as a positive regulator of sdsA and sdsB transcription. P(sdsA) and P(sdsB) activities rose significantly in the presence of the SDS, indicating inducibility of sdsA and sdsB transcription. DNA-binding assay indicated that SdsB directly regulates the transcription of sdsA and sdsB genes. Strain ATCC19151 grew in a sterile septic tank drain and on commercial detergent as sole source of carbon. Conclusions: SdsA enables Pseudomonas sp. ATCC19151 to utilize SDS as a sole carbon source. SdsB is positive transcriptional regulator of sdsA and sdsB genes. Significance and Impact of the Study: Ability of ATCC19151 to degrade anionic surfactants makes Pseudomonas sp. ATCC19151 a good candidate for bioremediation.
PB  - Wiley-Blackwell, Malden
T2  - Journal of Applied Microbiology
T1  - Regulation of the sdsA alkyl sulfatase of Pseudomonas sp ATCC19151 and its involvement in degradation of anionic surfactants
EP  - 1083
IS  - 3
SP  - 1076
VL  - 109
DO  - 10.1111/j.1365-2672.2010.04738.x
ER  - 

@article{
author = "Jovčić, Branko and Venturi, V. and Davison, J. and Topisirović, Ljubiša and Kojić, Milan",
year = "2010",
abstract = "Aims: The presented study was aimed to reveal transcriptional regulation of genes involved in SDS degradation (sdsA and sdsB) in Pseudomonas sp. ATCC19151. In addition, the ability of Pseudomonas sp. ATCC19151 to degrade anionic surfactants present in commercial detergent and septic tank drain was analysed. Methods and Results: Strain ATCC19151, at 30 degrees C, degrades all SDS present in the liquid medium (up to 4% w/v of SDS) within 48 h. ATCC19151 grows in the presence up to 15% (v/v) 'Fairy' commercial detergent and mineralizes 35% of present anionic surfactants. Analysis of the sdsA (P(sdsA)) and divergent sdsB (P(sdsB)) gene promoter activities revealed that SdsB acts as a positive regulator of sdsA and sdsB transcription. P(sdsA) and P(sdsB) activities rose significantly in the presence of the SDS, indicating inducibility of sdsA and sdsB transcription. DNA-binding assay indicated that SdsB directly regulates the transcription of sdsA and sdsB genes. Strain ATCC19151 grew in a sterile septic tank drain and on commercial detergent as sole source of carbon. Conclusions: SdsA enables Pseudomonas sp. ATCC19151 to utilize SDS as a sole carbon source. SdsB is positive transcriptional regulator of sdsA and sdsB genes. Significance and Impact of the Study: Ability of ATCC19151 to degrade anionic surfactants makes Pseudomonas sp. ATCC19151 a good candidate for bioremediation.",
publisher = "Wiley-Blackwell, Malden",
journal = "Journal of Applied Microbiology",
title = "Regulation of the sdsA alkyl sulfatase of Pseudomonas sp ATCC19151 and its involvement in degradation of anionic surfactants",
pages = "1083-1076",
number = "3",
volume = "109",
doi = "10.1111/j.1365-2672.2010.04738.x"
}

Jovčić, B., Venturi, V., Davison, J., Topisirović, L.,& Kojić, M.. (2010). Regulation of the sdsA alkyl sulfatase of Pseudomonas sp ATCC19151 and its involvement in degradation of anionic surfactants. in Journal of Applied Microbiology
Wiley-Blackwell, Malden., 109(3), 1076-1083.
https://doi.org/10.1111/j.1365-2672.2010.04738.x

Jovčić B, Venturi V, Davison J, Topisirović L, Kojić M. Regulation of the sdsA alkyl sulfatase of Pseudomonas sp ATCC19151 and its involvement in degradation of anionic surfactants. in Journal of Applied Microbiology. 2010;109(3):1076-1083.
doi:10.1111/j.1365-2672.2010.04738.x .

Jovčić, Branko, Venturi, V., Davison, J., Topisirović, Ljubiša, Kojić, Milan, "Regulation of the sdsA alkyl sulfatase of Pseudomonas sp ATCC19151 and its involvement in degradation of anionic surfactants" in Journal of Applied Microbiology, 109, no. 3 (2010):1076-1083,
https://doi.org/10.1111/j.1365-2672.2010.04738.x . .

TY  - JOUR
AU  - Živković, Milica
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/442
AB  - Ten lactobacilli and one leuconostoc showing auto-aggregation ability were isolated from artisanal cheeses. Furthermore, non-aggregation strains were isolated from the same cheese sample, if existed. The analysis of factor(s) possibly involved in auto-aggregation was performed. The pretreatment of cells with proteinase K resulted in the disappearance of auto-aggregation ability. Moreover, cells also lost aggregation ability after three-times, successive washing in distilled water. Testing the ability of strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 and its aggregation-deficient derivative BGSJ2-81 to co-aggregate with Listeria innocua ATCC33090, Escherichia coli ATCC25922 or Salmonella typhimurium TR251 showed that strain BGSJ2-8 co-aggregated with these strains, but derivative BGSJ2-81 was not. However, the treatment of L. paracasei subsp. paracasei BGSJ2-8 with proteinase K prior to co-aggregation tests resulted in losing co-aggregation ability. Surface properties of selected strains were analyzed by MATS (microbial adhesion to solvents) method. It was noticed that the strains with auto-aggregation ability were highly hydrophobic in comparison with aggregation-deficient ones. Comparative analyses of the surface features of strain L. paracasei subsp. paracasei BGSJ2-8 and its derivative BGSJ2-81 revealed notable difference.
PB  - Springer, New York
T2  - European Food Research and Technology
T1  - Surface properties of Lactobacillus and Leuconostoc isolates from homemade cheeses showing auto-aggregation ability
EP  - 931
IS  - 6
SP  - 925
VL  - 231
DO  - 10.1007/s00217-010-1344-1
ER  - 

@article{
author = "Živković, Milica and Jovčić, Branko and Kojić, Milan and Topisirović, Ljubiša",
year = "2010",
abstract = "Ten lactobacilli and one leuconostoc showing auto-aggregation ability were isolated from artisanal cheeses. Furthermore, non-aggregation strains were isolated from the same cheese sample, if existed. The analysis of factor(s) possibly involved in auto-aggregation was performed. The pretreatment of cells with proteinase K resulted in the disappearance of auto-aggregation ability. Moreover, cells also lost aggregation ability after three-times, successive washing in distilled water. Testing the ability of strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 and its aggregation-deficient derivative BGSJ2-81 to co-aggregate with Listeria innocua ATCC33090, Escherichia coli ATCC25922 or Salmonella typhimurium TR251 showed that strain BGSJ2-8 co-aggregated with these strains, but derivative BGSJ2-81 was not. However, the treatment of L. paracasei subsp. paracasei BGSJ2-8 with proteinase K prior to co-aggregation tests resulted in losing co-aggregation ability. Surface properties of selected strains were analyzed by MATS (microbial adhesion to solvents) method. It was noticed that the strains with auto-aggregation ability were highly hydrophobic in comparison with aggregation-deficient ones. Comparative analyses of the surface features of strain L. paracasei subsp. paracasei BGSJ2-8 and its derivative BGSJ2-81 revealed notable difference.",
publisher = "Springer, New York",
journal = "European Food Research and Technology",
title = "Surface properties of Lactobacillus and Leuconostoc isolates from homemade cheeses showing auto-aggregation ability",
pages = "931-925",
number = "6",
volume = "231",
doi = "10.1007/s00217-010-1344-1"
}

Živković, M., Jovčić, B., Kojić, M.,& Topisirović, L.. (2010). Surface properties of Lactobacillus and Leuconostoc isolates from homemade cheeses showing auto-aggregation ability. in European Food Research and Technology
Springer, New York., 231(6), 925-931.
https://doi.org/10.1007/s00217-010-1344-1

Živković M, Jovčić B, Kojić M, Topisirović L. Surface properties of Lactobacillus and Leuconostoc isolates from homemade cheeses showing auto-aggregation ability. in European Food Research and Technology. 2010;231(6):925-931.
doi:10.1007/s00217-010-1344-1 .

Živković, Milica, Jovčić, Branko, Kojić, Milan, Topisirović, Ljubiša, "Surface properties of Lactobacillus and Leuconostoc isolates from homemade cheeses showing auto-aggregation ability" in European Food Research and Technology, 231, no. 6 (2010):925-931,
https://doi.org/10.1007/s00217-010-1344-1 . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/421
AB  - The aim of this paper was to research the molecular cloning of genes encoding the novel bacteriocin BacSJ from Lactobacillus paracasei subsp. paracasei BGSJ2-8 by using a newly constructed shuttle cloning vector pA13. A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli, Lactobacillus and Lactococcus strains, showing a high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L. paracasei subsp. paracasei BGSJ2-8 was cloned in the pA13 using BamHI, obtaining the construct pB5. Sequencing and in silico analysis of the pB5 revealed 15 open reading frames (ORF). Plasmid pSJ2-8 harbors the genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. The combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled the determination of the primary structure of a bacteriocin BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirement for the production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to the class II bacteriocins. The shuttle cloning vector pA13 could be used as a tool for genetic manipulations in lactobacilli and lactococci.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912
EP  - 243
IS  - 2
SP  - 231
VL  - 62
DO  - 10.2298/ABS1002231K
ER  - 

@article{
author = "Kojić, Milan and Lozo, Jelena and Jovčić, Branko and Strahinić, Ivana and Fira, Đorđe and Topisirović, Ljubiša",
year = "2010",
abstract = "The aim of this paper was to research the molecular cloning of genes encoding the novel bacteriocin BacSJ from Lactobacillus paracasei subsp. paracasei BGSJ2-8 by using a newly constructed shuttle cloning vector pA13. A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli, Lactobacillus and Lactococcus strains, showing a high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L. paracasei subsp. paracasei BGSJ2-8 was cloned in the pA13 using BamHI, obtaining the construct pB5. Sequencing and in silico analysis of the pB5 revealed 15 open reading frames (ORF). Plasmid pSJ2-8 harbors the genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. The combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled the determination of the primary structure of a bacteriocin BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirement for the production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to the class II bacteriocins. The shuttle cloning vector pA13 could be used as a tool for genetic manipulations in lactobacilli and lactococci.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912",
pages = "243-231",
number = "2",
volume = "62",
doi = "10.2298/ABS1002231K"
}

Kojić, M., Lozo, J., Jovčić, B., Strahinić, I., Fira, Đ.,& Topisirović, L.. (2010). A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 231-243.
https://doi.org/10.2298/ABS1002231K

Kojić M, Lozo J, Jovčić B, Strahinić I, Fira Đ, Topisirović L. A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912. in Archives of Biological Sciences. 2010;62(2):231-243.
doi:10.2298/ABS1002231K .

Kojić, Milan, Lozo, Jelena, Jovčić, Branko, Strahinić, Ivana, Fira, Đorđe, Topisirović, Ljubiša, "A successful use of a new shuttle cloning vector pA13 for the cloning of the bacteriocins BacSJ and acidocin 8912" in Archives of Biological Sciences, 62, no. 2 (2010):231-243,
https://doi.org/10.2298/ABS1002231K . .

TY  - JOUR
AU  - Tolinački, Maja
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Topisirović, Ljubiša
AU  - Fira, Đorđe
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/405
AB  - The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9
EP  - 899
IS  - 4
SP  - 889
VL  - 62
DO  - 10.2298/ABS1004889T
ER  - 

@article{
author = "Tolinački, Maja and Kojić, Milan and Lozo, Jelena and Terzić-Vidojević, Amarela and Topisirović, Ljubiša and Fira, Đorđe",
year = "2010",
abstract = "The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9",
pages = "899-889",
number = "4",
volume = "62",
doi = "10.2298/ABS1004889T"
}

Tolinački, M., Kojić, M., Lozo, J., Terzić-Vidojević, A., Topisirović, L.,& Fira, Đ.. (2010). Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 889-899.
https://doi.org/10.2298/ABS1004889T

Tolinački M, Kojić M, Lozo J, Terzić-Vidojević A, Topisirović L, Fira Đ. Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences. 2010;62(4):889-899.
doi:10.2298/ABS1004889T .

Tolinački, Maja, Kojić, Milan, Lozo, Jelena, Terzić-Vidojević, Amarela, Topisirović, Ljubiša, Fira, Đorđe, "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9" in Archives of Biological Sciences, 62, no. 4 (2010):889-899,
https://doi.org/10.2298/ABS1004889T . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/432
AB  - A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli. Lactobacillus and Lactococcus strains. It showed high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L paracasei subsp. paracasei BGSJ2-8 was cloned into pA13 using BamHI to obtain the construct, pB5. Sequencing and in silico analysis of pB5 revealed fifteen open reading frames (ORF). Plasmid pSJ2-8 harbours genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. Combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled determination of the primary structure of bacteriocin BacSJ. The bacSJ2-8 gene encodes 68-amino-acid peptide with a double-glycine leader peptide consisting of 18 amino acids, followed by the orf2 (bacSJ2-8i) which encodes the immunity protein of BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirements for production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to class II bacteriocins.
PB  - Elsevier, Amsterdam
T2  - International Journal of Food Microbiology
T1  - Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8
EP  - 124
IS  - 2-3
SP  - 117
VL  - 140
DO  - 10.1016/j.ijfoodmicro.2010.04.010
ER  - 

@article{
author = "Kojić, Milan and Lozo, Jelena and Jovčić, Branko and Strahinić, Ivana and Fira, Đorđe and Topisirović, Ljubiša",
year = "2010",
abstract = "A new shuttle-cloning vector, pA13, was constructed and successfully introduced into Escherichia coli. Lactobacillus and Lactococcus strains. It showed high segregational and structural stability in all three hosts. The natural plasmid pSJ2-8 from L paracasei subsp. paracasei BGSJ2-8 was cloned into pA13 using BamHI to obtain the construct, pB5. Sequencing and in silico analysis of pB5 revealed fifteen open reading frames (ORF). Plasmid pSJ2-8 harbours genes encoding the production of two bacteriocins, BacSJ and acidocin 8912. Combined N-terminal amino acid sequencing of BacSJ in combination with DNA sequencing of the bacSJ2-8 gene enabled determination of the primary structure of bacteriocin BacSJ. The bacSJ2-8 gene encodes 68-amino-acid peptide with a double-glycine leader peptide consisting of 18 amino acids, followed by the orf2 (bacSJ2-8i) which encodes the immunity protein of BacSJ. The production and functional expression of BacSJ in homologous and heterologous hosts suggest that bacSJ2-8 and bacSJ2-8i together with the genes encoding the ABC transporter and accessory protein are the minimal requirements for production of BacSJ. Biochemical and genetic analyses showed that BacSJ belongs to class II bacteriocins.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Food Microbiology",
title = "Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8",
pages = "124-117",
number = "2-3",
volume = "140",
doi = "10.1016/j.ijfoodmicro.2010.04.010"
}

Kojić, M., Lozo, J., Jovčić, B., Strahinić, I., Fira, Đ.,& Topisirović, L.. (2010). Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8. in International Journal of Food Microbiology
Elsevier, Amsterdam., 140(2-3), 117-124.
https://doi.org/10.1016/j.ijfoodmicro.2010.04.010

Kojić M, Lozo J, Jovčić B, Strahinić I, Fira Đ, Topisirović L. Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8. in International Journal of Food Microbiology. 2010;140(2-3):117-124.
doi:10.1016/j.ijfoodmicro.2010.04.010 .

Kojić, Milan, Lozo, Jelena, Jovčić, Branko, Strahinić, Ivana, Fira, Đorđe, Topisirović, Ljubiša, "Construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from Lactobacillus paracasei subsp paracasei BGSJ2-8" in International Journal of Food Microbiology, 140, no. 2-3 (2010):117-124,
https://doi.org/10.1016/j.ijfoodmicro.2010.04.010 . .

TY  - JOUR
AU  - Begović, Jelena
AU  - Fira, Đorđe
AU  - Terzić-Vidojević, Amarela
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/414
AB  - Lactobacilli represent normal commensals of the human body, particularly in the gut and vagina where they protect these environments from incoming pathogens via a variety of mechanisms. The influence of the carbohydrate source present in reconstituted MRS growth medium on the different cell properties of two Lactobacillus rhamnosus strains were examined. Two human vaginal isolates, BGHV719 and exopolysaccharide producer strain BGHV954 were analyzed. The results demonstrated that unlike in reconstituted MRS with glucose as a carbon source, the presence of fructose, mannose, or rhamnose, significantly reduced cell surface hydrophobicity of both strains. In addition, differences in cell wall protein composition of L. rhamnosus BGHV719 and alterations in colony mucoidity of L. rhamnosus BGHV954 were also demonstrated. Light and SEM microscopy revealed differences on the cellular level when BGHV719 was cultivated in the presence of different sugars. The results of this study point out the importance of complex relationships between growth medium composition and the different aspects of bacterial behavior, and call for more detailed analyses of versatile bacterial responses to the changes in the environment, including vaginal ecosystem. This is especially important since lactobacilli are amongst the most widely used of probiotics.
PB  - SCIENDO, Warsaw
T2  - Central European Journal of Biology
T1  - Influence of carbohydrates on cell properties of Lactobacillus rhamnosus
EP  - 110
IS  - 1
SP  - 103
VL  - 5
DO  - 10.2478/s11535-009-0078-1
ER  - 

@article{
author = "Begović, Jelena and Fira, Đorđe and Terzić-Vidojević, Amarela and Topisirović, Ljubiša",
year = "2010",
abstract = "Lactobacilli represent normal commensals of the human body, particularly in the gut and vagina where they protect these environments from incoming pathogens via a variety of mechanisms. The influence of the carbohydrate source present in reconstituted MRS growth medium on the different cell properties of two Lactobacillus rhamnosus strains were examined. Two human vaginal isolates, BGHV719 and exopolysaccharide producer strain BGHV954 were analyzed. The results demonstrated that unlike in reconstituted MRS with glucose as a carbon source, the presence of fructose, mannose, or rhamnose, significantly reduced cell surface hydrophobicity of both strains. In addition, differences in cell wall protein composition of L. rhamnosus BGHV719 and alterations in colony mucoidity of L. rhamnosus BGHV954 were also demonstrated. Light and SEM microscopy revealed differences on the cellular level when BGHV719 was cultivated in the presence of different sugars. The results of this study point out the importance of complex relationships between growth medium composition and the different aspects of bacterial behavior, and call for more detailed analyses of versatile bacterial responses to the changes in the environment, including vaginal ecosystem. This is especially important since lactobacilli are amongst the most widely used of probiotics.",
publisher = "SCIENDO, Warsaw",
journal = "Central European Journal of Biology",
title = "Influence of carbohydrates on cell properties of Lactobacillus rhamnosus",
pages = "110-103",
number = "1",
volume = "5",
doi = "10.2478/s11535-009-0078-1"
}

Begović, J., Fira, Đ., Terzić-Vidojević, A.,& Topisirović, L.. (2010). Influence of carbohydrates on cell properties of Lactobacillus rhamnosus. in Central European Journal of Biology
SCIENDO, Warsaw., 5(1), 103-110.
https://doi.org/10.2478/s11535-009-0078-1

Begović J, Fira Đ, Terzić-Vidojević A, Topisirović L. Influence of carbohydrates on cell properties of Lactobacillus rhamnosus. in Central European Journal of Biology. 2010;5(1):103-110.
doi:10.2478/s11535-009-0078-1 .

Begović, Jelena, Fira, Đorđe, Terzić-Vidojević, Amarela, Topisirović, Ljubiša, "Influence of carbohydrates on cell properties of Lactobacillus rhamnosus" in Central European Journal of Biology, 5, no. 1 (2010):103-110,
https://doi.org/10.2478/s11535-009-0078-1 . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Kojić, Milan
AU  - Tolinački, Maja
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/465
AB  - Aims: The study of proteolytic activity and examination of proteinase gene region organization in proteolytically active Lactobacillus plantarum strains from different natural sources. Methods and Results: A set of 37 lactobacilli was distinguished by using multiplex PCR assay. Results showed that 34 strains were Lact. plantarum and three of them were Lact. paraplantarum. The examination of proteolytic activity revealed that 28 Lact. plantarum and two Lact. paraplantarum hydrolyse beta-casein. Further analyses of all proteolytically active Lact. plantarum with primers specific for different types of CEPs demonstrated that strain BGSJ3-18 has prtP catalytic domain as well as prtP-prtM intergenic region showing more than 95% sequence identity with the same regions present in Lact. paracasei, Lact. casei and L. lactis. No presence of prtB, prtH or prtR proteinase genes was detected in any of tested Lact. plantarum strains. Conclusions: One out of 28 analysed Lact. plantarum strains harbours the prtP-like gene. The other proteolytically active Lact. plantarum probably possesses a different type of extracellular proteinase(s). Significance and Impact of the Study: It is the first report about the presence of the prtP-like gene in Lact. plantarum, which illustrates the mobility of this gene and its presence in different species.
PB  - Wiley, Hoboken
T2  - Letters in Applied Microbiology
T1  - The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18
EP  - 49
IS  - 1
SP  - 43
VL  - 50
DO  - 10.1111/j.1472-765X.2009.02748.x
ER  - 

@article{
author = "Strahinić, Ivana and Kojić, Milan and Tolinački, Maja and Fira, Đorđe and Topisirović, Ljubiša",
year = "2010",
abstract = "Aims: The study of proteolytic activity and examination of proteinase gene region organization in proteolytically active Lactobacillus plantarum strains from different natural sources. Methods and Results: A set of 37 lactobacilli was distinguished by using multiplex PCR assay. Results showed that 34 strains were Lact. plantarum and three of them were Lact. paraplantarum. The examination of proteolytic activity revealed that 28 Lact. plantarum and two Lact. paraplantarum hydrolyse beta-casein. Further analyses of all proteolytically active Lact. plantarum with primers specific for different types of CEPs demonstrated that strain BGSJ3-18 has prtP catalytic domain as well as prtP-prtM intergenic region showing more than 95% sequence identity with the same regions present in Lact. paracasei, Lact. casei and L. lactis. No presence of prtB, prtH or prtR proteinase genes was detected in any of tested Lact. plantarum strains. Conclusions: One out of 28 analysed Lact. plantarum strains harbours the prtP-like gene. The other proteolytically active Lact. plantarum probably possesses a different type of extracellular proteinase(s). Significance and Impact of the Study: It is the first report about the presence of the prtP-like gene in Lact. plantarum, which illustrates the mobility of this gene and its presence in different species.",
publisher = "Wiley, Hoboken",
journal = "Letters in Applied Microbiology",
title = "The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18",
pages = "49-43",
number = "1",
volume = "50",
doi = "10.1111/j.1472-765X.2009.02748.x"
}

Strahinić, I., Kojić, M., Tolinački, M., Fira, Đ.,& Topisirović, L.. (2010). The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18. in Letters in Applied Microbiology
Wiley, Hoboken., 50(1), 43-49.
https://doi.org/10.1111/j.1472-765X.2009.02748.x

Strahinić I, Kojić M, Tolinački M, Fira Đ, Topisirović L. The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18. in Letters in Applied Microbiology. 2010;50(1):43-49.
doi:10.1111/j.1472-765X.2009.02748.x .

Strahinić, Ivana, Kojić, Milan, Tolinački, Maja, Fira, Đorđe, Topisirović, Ljubiša, "The presence of prtP proteinase gene in natural isolate Lactobacillus plantarum BGSJ3-18" in Letters in Applied Microbiology, 50, no. 1 (2010):43-49,
https://doi.org/10.1111/j.1472-765X.2009.02748.x . .

TY  - JOUR
AU  - Lakićević, Brankica
AU  - Stjepanović, Aleksandra
AU  - Milijašević, M.
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Topisirović, Ljubiša
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/402
AB  - The aim of the study was to establish a protocol to evaluate the presence of Listeria spp. in food processing environments. The presence of Listeria spp. was evaluated in a selected restaurant in Serbia on three occasions. Samples were collected from 47 sampling spots in the commercial kitchen equipment and environment. The presence of Listeria spp. and Listeria monocytogenes were detected by conventional culture methods and by the PCR method. The obtained results showed that 23 swab samples were positive for Listeria spp. Interestingly, the swabs from the bread-cutting board and meat defrosting sink were positive for L. monocytogenes.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The presence of Listeria spp. and Listeria monocytogenes in a chosen food processing establishment in Serbia
EP  - 887
IS  - 4
SP  - 881
VL  - 62
DO  - 10.2298/ABS1004881L
ER  - 

@article{
author = "Lakićević, Brankica and Stjepanović, Aleksandra and Milijašević, M. and Terzić-Vidojević, Amarela and Golić, Nataša and Topisirović, Ljubiša",
year = "2010",
abstract = "The aim of the study was to establish a protocol to evaluate the presence of Listeria spp. in food processing environments. The presence of Listeria spp. was evaluated in a selected restaurant in Serbia on three occasions. Samples were collected from 47 sampling spots in the commercial kitchen equipment and environment. The presence of Listeria spp. and Listeria monocytogenes were detected by conventional culture methods and by the PCR method. The obtained results showed that 23 swab samples were positive for Listeria spp. Interestingly, the swabs from the bread-cutting board and meat defrosting sink were positive for L. monocytogenes.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The presence of Listeria spp. and Listeria monocytogenes in a chosen food processing establishment in Serbia",
pages = "887-881",
number = "4",
volume = "62",
doi = "10.2298/ABS1004881L"
}

Lakićević, B., Stjepanović, A., Milijašević, M., Terzić-Vidojević, A., Golić, N.,& Topisirović, L.. (2010). The presence of Listeria spp. and Listeria monocytogenes in a chosen food processing establishment in Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 881-887.
https://doi.org/10.2298/ABS1004881L

Lakićević B, Stjepanović A, Milijašević M, Terzić-Vidojević A, Golić N, Topisirović L. The presence of Listeria spp. and Listeria monocytogenes in a chosen food processing establishment in Serbia. in Archives of Biological Sciences. 2010;62(4):881-887.
doi:10.2298/ABS1004881L .

Lakićević, Brankica, Stjepanović, Aleksandra, Milijašević, M., Terzić-Vidojević, Amarela, Golić, Nataša, Topisirović, Ljubiša, "The presence of Listeria spp. and Listeria monocytogenes in a chosen food processing establishment in Serbia" in Archives of Biological Sciences, 62, no. 4 (2010):881-887,
https://doi.org/10.2298/ABS1004881L . .

TY  - JOUR
AU  - Begović, Jelena
AU  - Huys, Geert
AU  - Mayo, Baltasar
AU  - D'Haene, Klaas
AU  - Belen Florez, Ana
AU  - Lozo, Jelena
AU  - Kojić, Milan
AU  - Strahinić, Ivana
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/396
AB  - Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance
EP  - 426
IS  - 6
SP  - 421
VL  - 160
DO  - 10.1016/j.resmic.2009.07.005
ER  - 

@article{
author = "Begović, Jelena and Huys, Geert and Mayo, Baltasar and D'Haene, Klaas and Belen Florez, Ana and Lozo, Jelena and Kojić, Milan and Strahinić, Ivana and Topisirović, Ljubiša",
year = "2009",
abstract = "Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance",
pages = "426-421",
number = "6",
volume = "160",
doi = "10.1016/j.resmic.2009.07.005"
}

Begović, J., Huys, G., Mayo, B., D'Haene, K., Belen Florez, A., Lozo, J., Kojić, M., Strahinić, I.,& Topisirović, L.. (2009). Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology
Elsevier, Amsterdam., 160(6), 421-426.
https://doi.org/10.1016/j.resmic.2009.07.005

Begović J, Huys G, Mayo B, D'Haene K, Belen Florez A, Lozo J, Kojić M, Strahinić I, Topisirović L. Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology. 2009;160(6):421-426.
doi:10.1016/j.resmic.2009.07.005 .

Begović, Jelena, Huys, Geert, Mayo, Baltasar, D'Haene, Klaas, Belen Florez, Ana, Lozo, Jelena, Kojić, Milan, Strahinić, Ivana, Topisirović, Ljubiša, "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance" in Research in Microbiology, 160, no. 6 (2009):421-426,
https://doi.org/10.1016/j.resmic.2009.07.005 . .

TY  - JOUR
AU  - Sanchez Valenzuela, Antonio
AU  - ben Omar, Nabil
AU  - Abriouel, Hikmate
AU  - Lucas Lopez, Rosario
AU  - Veljović, Katarina
AU  - Martinez Canamero, Magdalena
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/367
AB  - A collection of 25 isolates from foods of animal origin (including mainly milk and cheese, together with meat and ham) was studied. Enterococci were identified at species levels as E. faecalis (9 isolates) and E. faecium (16 isolates). Investigation of virulence factors by PCR amplification revealed incomplete sets of cytolysin genes both in E faecalis and E. faecium isolates. Among E. faecalis, PCR amplification revealed a high incidence of genes encoding for enterococcal surface protein esp (7/9 isolates), enterococcal antigen efaA(fs) (6/9), aggregation substance agg (2/9) and sex-pheromone encoding genes ccf, cob, cpd (which were detected in 9, 5 and 3 out of 9 isolates, respectively). By contrast, only esp (7/16 isolates) and efaA(fm) (10/16) were detected among E.faecium. Antibiotic resistance detected at higher frequencies included rifampicin, nitrofurantoin, ciprofloxacin and levofloxacin. Vancomycin resistance was also detected among E.faecalis and E.faecium. E.faecalis isolates showed decarboxylating activity mostly for tyrosine (5/9 isolates), while E faecium isolates showed a broader decarboxylating capacity, involving tyrosine (11/16 isolates) ornithine (6/16), lysine (4/16) and histidine (3/16). Six isolates produced bacteriocins, and genes encoding for enterocins A, B, P, L50, and 1071 were detected. Many isolates tested positive for several of the traits investigated, which raises concerns about their possible role as reservoirs for dissemination of antibiotic resistance and virulence traits in foods.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin
EP  - 385
IS  - 4
SP  - 381
VL  - 20
DO  - 10.1016/j.foodcont.2008.06.004
ER  - 

@article{
author = "Sanchez Valenzuela, Antonio and ben Omar, Nabil and Abriouel, Hikmate and Lucas Lopez, Rosario and Veljović, Katarina and Martinez Canamero, Magdalena and Topisirović, Ljubiša and Kojić, Milan",
year = "2009",
abstract = "A collection of 25 isolates from foods of animal origin (including mainly milk and cheese, together with meat and ham) was studied. Enterococci were identified at species levels as E. faecalis (9 isolates) and E. faecium (16 isolates). Investigation of virulence factors by PCR amplification revealed incomplete sets of cytolysin genes both in E faecalis and E. faecium isolates. Among E. faecalis, PCR amplification revealed a high incidence of genes encoding for enterococcal surface protein esp (7/9 isolates), enterococcal antigen efaA(fs) (6/9), aggregation substance agg (2/9) and sex-pheromone encoding genes ccf, cob, cpd (which were detected in 9, 5 and 3 out of 9 isolates, respectively). By contrast, only esp (7/16 isolates) and efaA(fm) (10/16) were detected among E.faecium. Antibiotic resistance detected at higher frequencies included rifampicin, nitrofurantoin, ciprofloxacin and levofloxacin. Vancomycin resistance was also detected among E.faecalis and E.faecium. E.faecalis isolates showed decarboxylating activity mostly for tyrosine (5/9 isolates), while E faecium isolates showed a broader decarboxylating capacity, involving tyrosine (11/16 isolates) ornithine (6/16), lysine (4/16) and histidine (3/16). Six isolates produced bacteriocins, and genes encoding for enterocins A, B, P, L50, and 1071 were detected. Many isolates tested positive for several of the traits investigated, which raises concerns about their possible role as reservoirs for dissemination of antibiotic resistance and virulence traits in foods.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin",
pages = "385-381",
number = "4",
volume = "20",
doi = "10.1016/j.foodcont.2008.06.004"
}

Sanchez Valenzuela, A., ben Omar, N., Abriouel, H., Lucas Lopez, R., Veljović, K., Martinez Canamero, M., Topisirović, L.,& Kojić, M.. (2009). Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin. in Food Control
Elsevier Sci Ltd, Oxford., 20(4), 381-385.
https://doi.org/10.1016/j.foodcont.2008.06.004

Sanchez Valenzuela A, ben Omar N, Abriouel H, Lucas Lopez R, Veljović K, Martinez Canamero M, Topisirović L, Kojić M. Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin. in Food Control. 2009;20(4):381-385.
doi:10.1016/j.foodcont.2008.06.004 .

Sanchez Valenzuela, Antonio, ben Omar, Nabil, Abriouel, Hikmate, Lucas Lopez, Rosario, Veljović, Katarina, Martinez Canamero, Magdalena, Topisirović, Ljubiša, Kojić, Milan, "Virulence factors, antibiotic resistance, and bacteriocins in enterococci from artisan foods of animal origin" in Food Control, 20, no. 4 (2009):381-385,
https://doi.org/10.1016/j.foodcont.2008.06.004 . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Kojić, Milan
AU  - Tolinački, Maja
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/366
AB  - Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors
EP  - 88
IS  - 1
SP  - 78
VL  - 106
DO  - 10.1111/j.1365-2672.2008.03977.x
ER  - 

@article{
author = "Strahinić, Ivana and Kojić, Milan and Tolinački, Maja and Fira, Đorđe and Topisirović, Ljubiša",
year = "2009",
abstract = "Strain Lactococcus lactis subsp. lactis bv. diacetylactis S50 harbours five theta-replicating plasmids (pS6, pS7a, pS7b, pS80 and pS140). The aim of this study was to characterize domains involved in the replication and conjugative mobilization of the small plasmids pS7a and pS7b, which are structurally very similar. Complete nucleotide sequences of pS7a and pS7b were determined by cloning DNA fragments of different sizes into Escherichia coli vectors. Linearized plasmids and four EcoRI fragments of the pS7a and pS7b were cloned into an origin probe vector. Constructed plasmids (pSEV10, pSK10, pISE1a and pISE1b) were able to replicate in the strain L. lactis subsp. cremoris MG1363. In addition, experiments showed that plasmids pS7a and pS7b contained oriT sequences and their conjugative transfer directly depended on the presence of pS80 in donor cells. Plasmids pS7a and pS7b contained typical lactococcal theta replication origin and repB gene that enable them to replicate in the strain L. lactis subsp. cremoris MG1363. Plasmid pS80 plays a key role in the conjugative transfer of small plasmids. Plasmids pS7a and pS7b-based derivatives could be valuable tools for genetic manipulation, studying processes of plasmid maintenance and horizontal gene transfer in lactococci.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors",
pages = "88-78",
number = "1",
volume = "106",
doi = "10.1111/j.1365-2672.2008.03977.x"
}

Strahinić, I., Kojić, M., Tolinački, M., Fira, Đ.,& Topisirović, L.. (2009). Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. in Journal of Applied Microbiology
Wiley, Hoboken., 106(1), 78-88.
https://doi.org/10.1111/j.1365-2672.2008.03977.x

Strahinić I, Kojić M, Tolinački M, Fira Đ, Topisirović L. Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors. in Journal of Applied Microbiology. 2009;106(1):78-88.
doi:10.1111/j.1365-2672.2008.03977.x .

Strahinić, Ivana, Kojić, Milan, Tolinački, Maja, Fira, Đorđe, Topisirović, Ljubiša, "Molecular characterization of plasmids pS7a and pS7b from Lactococcus lactis subsp lactis bv. diacetylactis S50 as a base for the construction of mobilizable cloning vectors" in Journal of Applied Microbiology, 106, no. 1 (2009):78-88,
https://doi.org/10.1111/j.1365-2672.2008.03977.x . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Ostojić, Mihailo
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/352
AB  - Iz belog polutvrdog zlatarskog sira, označenog kao BGNV, izolovan je osamdeset jedan soj bakterija mlečne kiseline (BMK). Sir je uzet iz jednog seoskog domaćinstva smeštenog na severnoj strani planine Zlatar. Zlatarski BGNV sir je napravljen od svežeg kravljeg mleka bez dodatka starter kulture. Svi izolati BMK su okarakterisani fenotipskim i genotipskim testovima. Identifikacija sojeva je rađena rep-PCR analizom sa (GTG)5 prajmerom i 16S rDNK sekvenciranjem. Najzastupljenije vrste u zlatarskom BGNV siru su bile Lactobacillus casei/paracasei (65.43%) i Enterococcus faecalis (29.63%. Dva fakultetivno anaerobna štapića su identifikovana kao Lactobacillus plantarum (2.47%), a dva obligatna heterofermentativna izolata BMK su identifikovani kao Lactobacillus parabuchneri (2.47%). Od svih 81 testiranih izolata, samo osam eneterokoka su bili proizvođači antimikrobnih komponenti. Četrnaest od 16 testiranih izolata laktobacila je pokazivalo srednju do vrlo dobru proteolitičku aktivnost. Svih 57 laktobacila iz zlatarskog BGNV sira veoma sporo grušaju mleko, ili ga uopšte ne grušaju. Međutim, tri izolata enterokoka, BGNV1-63, BGNV1-76 i BGNV1-80, su pokazivala vrlo dobru aktivnost u mleku i grušala su ga za 5 h. Ove enterokoke su pokazivale vrlo visoku proteolitičku aktivnost potpuno hidrolizirajući αs1- i κ- kazein nakon 3 h, a β-kazein nakon 30 min inkubacije. Pored toga, ova tri izolata enterokoka degradovala su želatin. Upoređujući dobijene rezultate sa onima prethodno dobijenim ispitivanjem BMK u drugom zlatarskom BGZLS siru, napravljenom takođe, od svežeg kravljeg mleka, može se zaključiti da je mikroflora BMK zlatraskog BGNV sira manje raznovrsna.
AB  - Eighty-one strains of lactic acid bacteria (LAB) were isolated from white semi-hard homemade cheese, designated Zlatar BGNV, which was taken from household settled on Northern side of mountain Zlatar. The Zlatar BGNV cheese was manufactured from raw cow's milk without addition of the starter culture. All isolates of LAB were characterized by phenotypic and genotypic tests. Identification of strains was done by the repetitive extragenic palindromic-polymerase chain reaction (rep-PCR) with (GTG)5 primer and 16S rDNA sequence analysis. The most present species in Zlatar BGNV cheese were Lactobacillus casei/paracasei (65.43%) and Enterococcus faecalis (29.63%). Two facultative heterofermentative rods were identified as Lactobacillus plantarum (2.47%), and two obligate hetrofermentative LAB isolates as Lactobacillus parabuchneri (2.47%). Among all 81 tested isolates, only eight enterococci were producers of antimicrobial compounds. Fourteen of 16 tested lactobacilli isolates showed medium to very good proteolytic activity. All 57 lactobacilli from the Zlatar BGNV cheese curdled milk very slowly or did not curdle milk at all. However, three isolates of enterococci, BGNV1-63, BGNV1-76 and BGNV1-80, showed very good activity in milk and curdled milk within 5 h. They showed very high proteolytic activity hydrolyzing completely αs1- and κ-casein after 3 h, and β-casein after 30 min of incubation. In addition, those three enterococcal isolates degraded gelatin. Comparing obtained results with those previously achieved in examination of LAB microflora in another Zlatar BGZLS cheese made also from raw cow's milk, it can be concluded that LAB microflora in the Zlatar BGNV cheese is less diverse.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Karakterizacija bakterija mlečne kiseline izolovanih iz zlatarskih sireva proizvedenih u domaćinstvu na dve geografski različite lokacije
T1  - Characterization of lactic acid bacteria isolated from artisanal Zlatar cheeses produced at two different geographical location
EP  - 136
IS  - 1
SP  - 117
VL  - 41
DO  - 10.2298/GENSR0901117T
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Ostojić, Mihailo and Topisirović, Ljubiša",
year = "2009",
abstract = "Iz belog polutvrdog zlatarskog sira, označenog kao BGNV, izolovan je osamdeset jedan soj bakterija mlečne kiseline (BMK). Sir je uzet iz jednog seoskog domaćinstva smeštenog na severnoj strani planine Zlatar. Zlatarski BGNV sir je napravljen od svežeg kravljeg mleka bez dodatka starter kulture. Svi izolati BMK su okarakterisani fenotipskim i genotipskim testovima. Identifikacija sojeva je rađena rep-PCR analizom sa (GTG)5 prajmerom i 16S rDNK sekvenciranjem. Najzastupljenije vrste u zlatarskom BGNV siru su bile Lactobacillus casei/paracasei (65.43%) i Enterococcus faecalis (29.63%. Dva fakultetivno anaerobna štapića su identifikovana kao Lactobacillus plantarum (2.47%), a dva obligatna heterofermentativna izolata BMK su identifikovani kao Lactobacillus parabuchneri (2.47%). Od svih 81 testiranih izolata, samo osam eneterokoka su bili proizvođači antimikrobnih komponenti. Četrnaest od 16 testiranih izolata laktobacila je pokazivalo srednju do vrlo dobru proteolitičku aktivnost. Svih 57 laktobacila iz zlatarskog BGNV sira veoma sporo grušaju mleko, ili ga uopšte ne grušaju. Međutim, tri izolata enterokoka, BGNV1-63, BGNV1-76 i BGNV1-80, su pokazivala vrlo dobru aktivnost u mleku i grušala su ga za 5 h. Ove enterokoke su pokazivale vrlo visoku proteolitičku aktivnost potpuno hidrolizirajući αs1- i κ- kazein nakon 3 h, a β-kazein nakon 30 min inkubacije. Pored toga, ova tri izolata enterokoka degradovala su želatin. Upoređujući dobijene rezultate sa onima prethodno dobijenim ispitivanjem BMK u drugom zlatarskom BGZLS siru, napravljenom takođe, od svežeg kravljeg mleka, može se zaključiti da je mikroflora BMK zlatraskog BGNV sira manje raznovrsna., Eighty-one strains of lactic acid bacteria (LAB) were isolated from white semi-hard homemade cheese, designated Zlatar BGNV, which was taken from household settled on Northern side of mountain Zlatar. The Zlatar BGNV cheese was manufactured from raw cow's milk without addition of the starter culture. All isolates of LAB were characterized by phenotypic and genotypic tests. Identification of strains was done by the repetitive extragenic palindromic-polymerase chain reaction (rep-PCR) with (GTG)5 primer and 16S rDNA sequence analysis. The most present species in Zlatar BGNV cheese were Lactobacillus casei/paracasei (65.43%) and Enterococcus faecalis (29.63%). Two facultative heterofermentative rods were identified as Lactobacillus plantarum (2.47%), and two obligate hetrofermentative LAB isolates as Lactobacillus parabuchneri (2.47%). Among all 81 tested isolates, only eight enterococci were producers of antimicrobial compounds. Fourteen of 16 tested lactobacilli isolates showed medium to very good proteolytic activity. All 57 lactobacilli from the Zlatar BGNV cheese curdled milk very slowly or did not curdle milk at all. However, three isolates of enterococci, BGNV1-63, BGNV1-76 and BGNV1-80, showed very good activity in milk and curdled milk within 5 h. They showed very high proteolytic activity hydrolyzing completely αs1- and κ-casein after 3 h, and β-casein after 30 min of incubation. In addition, those three enterococcal isolates degraded gelatin. Comparing obtained results with those previously achieved in examination of LAB microflora in another Zlatar BGZLS cheese made also from raw cow's milk, it can be concluded that LAB microflora in the Zlatar BGNV cheese is less diverse.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Karakterizacija bakterija mlečne kiseline izolovanih iz zlatarskih sireva proizvedenih u domaćinstvu na dve geografski različite lokacije, Characterization of lactic acid bacteria isolated from artisanal Zlatar cheeses produced at two different geographical location",
pages = "136-117",
number = "1",
volume = "41",
doi = "10.2298/GENSR0901117T"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Ostojić, M.,& Topisirović, L.. (2009). Karakterizacija bakterija mlečne kiseline izolovanih iz zlatarskih sireva proizvedenih u domaćinstvu na dve geografski različite lokacije. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 41(1), 117-136.
https://doi.org/10.2298/GENSR0901117T

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Ostojić M, Topisirović L. Karakterizacija bakterija mlečne kiseline izolovanih iz zlatarskih sireva proizvedenih u domaćinstvu na dve geografski različite lokacije. in Genetika-Belgrade. 2009;41(1):117-136.
doi:10.2298/GENSR0901117T .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Ostojić, Mihailo, Topisirović, Ljubiša, "Karakterizacija bakterija mlečne kiseline izolovanih iz zlatarskih sireva proizvedenih u domaćinstvu na dve geografski različite lokacije" in Genetika-Belgrade, 41, no. 1 (2009):117-136,
https://doi.org/10.2298/GENSR0901117T . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Begović, Jelena
AU  - Lozo, Jelena
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/345
AB  - Poznato je da soj Pseudomonas sp. ATCC19151 poseduje gen koji kodira Potencijalnu alkilsulfatazu. U ovom radu analizirana je sposobnost rasta ovog soja u minimalnom medijum usa različitim koncentracijama natrijum dodecilsulfata (0.5, 0.75 i 1 %) kao jedinim izvorom ugljenika. Pokazano je da Pseudomonas sp. ATCC19151 ispoljava najbolji rast uminimalnom medijumusa 0.5 % natrijum dodecil sulfata, te je stoga ova koncentracija uzeta kao optimalna za testiranje dinamike korišćenja natrijum dodecil sulfata tokom različitih faza rasta. Dinamika korišćenja natrijum dodecil sulfata podudarala se sa rastom kulture. Pored toga u cilju detaljnije karakterizacije soja, analizirana je i osetljivost Pseudomonas sp. ATCC19151 na antibiotike. Pokazano je da je analizirani soj rezistentan na šest (ampicilin, tetraciklin, hloramfenikol, tobramicin, nalidiksičnukiselinui gentamicin) od devet analiziranih antibiotika.
AB  - Pseudomonas sp. ATCC19151 harbors a gene encoding a putative alkylsulfatase (sdsA). Here we report a growth ability of this strain in minimal media containing 0.5, 0.75, and 1% sodium dodecyl sulfate as the sole carbon source. The most prominent growth was detected for the minimal medium with 0.5% SDS, so this concentration of SDS was used to monitor Pseudomonas sp. ATCC19151 SDS biodegradation dynamics. Bacterial growth coincided with the disappearance of SDS. Antibiotic susceptibility was tested as well. Pseudomonas sp. ATCC19151 was resistant to six out of nine tested antibiotics, including ampicillin, tetracycline, chloramphenicol, tobramycin, nalidixic acid, and gentamycin.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Dinamika korišćenja natrijum dodecil sulfata i osetljivost na antibiotike soja Pseudomonas sp. ATCC19151
T1  - Dynamics of sodium dodecyl sulfate utilization andantibiotic susceptibility of strain Pseudomonas sp. ATCC19151
EP  - 164
IS  - 2
SP  - 159
VL  - 61
DO  - 10.2298/ABS0902159J
ER  - 

@article{
author = "Jovčić, Branko and Begović, Jelena and Lozo, Jelena and Topisirović, Ljubiša and Kojić, Milan",
year = "2009",
abstract = "Poznato je da soj Pseudomonas sp. ATCC19151 poseduje gen koji kodira Potencijalnu alkilsulfatazu. U ovom radu analizirana je sposobnost rasta ovog soja u minimalnom medijum usa različitim koncentracijama natrijum dodecilsulfata (0.5, 0.75 i 1 %) kao jedinim izvorom ugljenika. Pokazano je da Pseudomonas sp. ATCC19151 ispoljava najbolji rast uminimalnom medijumusa 0.5 % natrijum dodecil sulfata, te je stoga ova koncentracija uzeta kao optimalna za testiranje dinamike korišćenja natrijum dodecil sulfata tokom različitih faza rasta. Dinamika korišćenja natrijum dodecil sulfata podudarala se sa rastom kulture. Pored toga u cilju detaljnije karakterizacije soja, analizirana je i osetljivost Pseudomonas sp. ATCC19151 na antibiotike. Pokazano je da je analizirani soj rezistentan na šest (ampicilin, tetraciklin, hloramfenikol, tobramicin, nalidiksičnukiselinui gentamicin) od devet analiziranih antibiotika., Pseudomonas sp. ATCC19151 harbors a gene encoding a putative alkylsulfatase (sdsA). Here we report a growth ability of this strain in minimal media containing 0.5, 0.75, and 1% sodium dodecyl sulfate as the sole carbon source. The most prominent growth was detected for the minimal medium with 0.5% SDS, so this concentration of SDS was used to monitor Pseudomonas sp. ATCC19151 SDS biodegradation dynamics. Bacterial growth coincided with the disappearance of SDS. Antibiotic susceptibility was tested as well. Pseudomonas sp. ATCC19151 was resistant to six out of nine tested antibiotics, including ampicillin, tetracycline, chloramphenicol, tobramycin, nalidixic acid, and gentamycin.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Dinamika korišćenja natrijum dodecil sulfata i osetljivost na antibiotike soja Pseudomonas sp. ATCC19151, Dynamics of sodium dodecyl sulfate utilization andantibiotic susceptibility of strain Pseudomonas sp. ATCC19151",
pages = "164-159",
number = "2",
volume = "61",
doi = "10.2298/ABS0902159J"
}

Jovčić, B., Begović, J., Lozo, J., Topisirović, L.,& Kojić, M.. (2009). Dinamika korišćenja natrijum dodecil sulfata i osetljivost na antibiotike soja Pseudomonas sp. ATCC19151. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 61(2), 159-164.
https://doi.org/10.2298/ABS0902159J

Jovčić B, Begović J, Lozo J, Topisirović L, Kojić M. Dinamika korišćenja natrijum dodecil sulfata i osetljivost na antibiotike soja Pseudomonas sp. ATCC19151. in Archives of Biological Sciences. 2009;61(2):159-164.
doi:10.2298/ABS0902159J .

Jovčić, Branko, Begović, Jelena, Lozo, Jelena, Topisirović, Ljubiša, Kojić, Milan, "Dinamika korišćenja natrijum dodecil sulfata i osetljivost na antibiotike soja Pseudomonas sp. ATCC19151" in Archives of Biological Sciences, 61, no. 2 (2009):159-164,
https://doi.org/10.2298/ABS0902159J . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Živković, Milica
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Busarčević, M.
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/389
AB  - Iz tri ručno pravljena sira uzeta iz različitih domaćinstava smeštenih u regionu planine Kavkaz, izolovano je ukupno 157 bakterija mleč ne kiseline (LAB). Sirevi su pravljeni od kravljeg mleka bez dodatka starter kulture. Izolati LAB su okarakterisani fenotipskim i genotipskim testovima. Rezultati identifikacije LAB pokazuju da je u ispitivanim sirevima prisutno deset vrsta, kao što su: Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus farciminis, Lactobacillus brevis, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc pseudomesenteroides, Enterococcus faecium i Enterococcus faecalis. Sojevi u okviru vrsta L. plantarum, L. arizonensis, L. paraplantarum, L. farciminis i L. pseudomesenteroides su pokazivali dobru proteolitičku aktivnost.
AB  - A total of 157 lactic acid bacteria (LAB) were isolated from three hand-made cheeses taken from different households in the region of the Caucasus Mountains. The cheeses were manufactured from cow's milk without the addition of a starter culture. The isolates of LAB were characterized by subjecting them to phenotypic and genotypic tests. The results of identification of LAB indicate that the examined cheeses contained 10 species, viz., Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus farciminis, Lactobacillus brevis, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc pseudomesenteroides, Enterococcus faecium, and Enterococcus faecalis. The strains within the species L. plantarum, L. arizonensis, L. paraplantarum, L. farciminis, and L. pseudomesenteroides showed good proteolytic activity.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Analiza mikroflore bakterija mlečne kiseline u tradicionalnim kravljim sirevima sa Kavkaza
T1  - Analysis of the lactic acid bacteria microflora in traditional Caucasus cow's milk cheeses
EP  - 406
IS  - 3
SP  - 395
VL  - 61
DO  - 10.2298/ABS0903395T
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Živković, Milica and Veljović, Katarina and Tolinački, Maja and Busarčević, M. and Topisirović, Ljubiša",
year = "2009",
abstract = "Iz tri ručno pravljena sira uzeta iz različitih domaćinstava smeštenih u regionu planine Kavkaz, izolovano je ukupno 157 bakterija mleč ne kiseline (LAB). Sirevi su pravljeni od kravljeg mleka bez dodatka starter kulture. Izolati LAB su okarakterisani fenotipskim i genotipskim testovima. Rezultati identifikacije LAB pokazuju da je u ispitivanim sirevima prisutno deset vrsta, kao što su: Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus farciminis, Lactobacillus brevis, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc pseudomesenteroides, Enterococcus faecium i Enterococcus faecalis. Sojevi u okviru vrsta L. plantarum, L. arizonensis, L. paraplantarum, L. farciminis i L. pseudomesenteroides su pokazivali dobru proteolitičku aktivnost., A total of 157 lactic acid bacteria (LAB) were isolated from three hand-made cheeses taken from different households in the region of the Caucasus Mountains. The cheeses were manufactured from cow's milk without the addition of a starter culture. The isolates of LAB were characterized by subjecting them to phenotypic and genotypic tests. The results of identification of LAB indicate that the examined cheeses contained 10 species, viz., Lactobacillus plantarum, Lactobacillus paraplantarum, Lactobacillus arizonensis, Lactobacillus farciminis, Lactobacillus brevis, Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides subsp. mesenteroides, Leuconostoc pseudomesenteroides, Enterococcus faecium, and Enterococcus faecalis. The strains within the species L. plantarum, L. arizonensis, L. paraplantarum, L. farciminis, and L. pseudomesenteroides showed good proteolytic activity.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Analiza mikroflore bakterija mlečne kiseline u tradicionalnim kravljim sirevima sa Kavkaza, Analysis of the lactic acid bacteria microflora in traditional Caucasus cow's milk cheeses",
pages = "406-395",
number = "3",
volume = "61",
doi = "10.2298/ABS0903395T"
}

Terzić-Vidojević, A., Živković, M., Veljović, K., Tolinački, M., Busarčević, M.,& Topisirović, L.. (2009). Analiza mikroflore bakterija mlečne kiseline u tradicionalnim kravljim sirevima sa Kavkaza. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 61(3), 395-406.
https://doi.org/10.2298/ABS0903395T

Terzić-Vidojević A, Živković M, Veljović K, Tolinački M, Busarčević M, Topisirović L. Analiza mikroflore bakterija mlečne kiseline u tradicionalnim kravljim sirevima sa Kavkaza. in Archives of Biological Sciences. 2009;61(3):395-406.
doi:10.2298/ABS0903395T .

Terzić-Vidojević, Amarela, Živković, Milica, Veljović, Katarina, Tolinački, Maja, Busarčević, M., Topisirović, Ljubiša, "Analiza mikroflore bakterija mlečne kiseline u tradicionalnim kravljim sirevima sa Kavkaza" in Archives of Biological Sciences, 61, no. 3 (2009):395-406,
https://doi.org/10.2298/ABS0903395T . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lozo, Jelena
AU  - Begović, Jelena
AU  - Gulahmadov, Sahib Gurban Oglu
AU  - Kuliev, Akif Alekperovich
AU  - Dalgalarrondo, Michele
AU  - Chobert, Jean-Marc
AU  - Haertle, Thomas
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/392
AB  - Studied lactic acid bacteria (LAB) were isolated from two types of final ready-to-eat artisanal dairy products (cheeses-Agdas, Sheki and yogurts-Karabakh, Ganja and Baku) manufactured in Azerbaijan. The Agdas cheese belongs to the group of semi hard cheeses whilst the Sheki cheese belongs to hard cheeses. Both of cheeses were produced from cow's milk without the addition of the starter cultures. The Karabakh and Baku yogurts were produced from bovine's milk and the Ganja yogurt from buffalo's milk. Overall 378 isolates were collected from these dairy products and 296 of them were Gram-positive and catalase-negative. It was determined using biochemical tests and molecular methods that four species of LAB were mostly present in these cheeses: Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus paraplantarum and Enterococcus faecium while in yogurts, Lactobacillus delbrueckii subsp. lactis, Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus and Enterococcus faecium dominated. Five enterococci were producers of antimicrobial compounds.
T2  - African Journal of Biotechnology
T1  - Phenotypic and genotypic characterization of lactic acid bacteria isolated from Azerbaijani traditional dairy products
EP  - 2588
IS  - 11
SP  - 2576
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_imagine_392
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Tolinački, Maja and Živković, Milica and Lozo, Jelena and Begović, Jelena and Gulahmadov, Sahib Gurban Oglu and Kuliev, Akif Alekperovich and Dalgalarrondo, Michele and Chobert, Jean-Marc and Haertle, Thomas and Topisirović, Ljubiša",
year = "2009",
abstract = "Studied lactic acid bacteria (LAB) were isolated from two types of final ready-to-eat artisanal dairy products (cheeses-Agdas, Sheki and yogurts-Karabakh, Ganja and Baku) manufactured in Azerbaijan. The Agdas cheese belongs to the group of semi hard cheeses whilst the Sheki cheese belongs to hard cheeses. Both of cheeses were produced from cow's milk without the addition of the starter cultures. The Karabakh and Baku yogurts were produced from bovine's milk and the Ganja yogurt from buffalo's milk. Overall 378 isolates were collected from these dairy products and 296 of them were Gram-positive and catalase-negative. It was determined using biochemical tests and molecular methods that four species of LAB were mostly present in these cheeses: Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus paraplantarum and Enterococcus faecium while in yogurts, Lactobacillus delbrueckii subsp. lactis, Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus and Enterococcus faecium dominated. Five enterococci were producers of antimicrobial compounds.",
journal = "African Journal of Biotechnology",
title = "Phenotypic and genotypic characterization of lactic acid bacteria isolated from Azerbaijani traditional dairy products",
pages = "2588-2576",
number = "11",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_imagine_392"
}

Terzić-Vidojević, A., Tolinački, M., Živković, M., Lozo, J., Begović, J., Gulahmadov, S. G. O., Kuliev, A. A., Dalgalarrondo, M., Chobert, J., Haertle, T.,& Topisirović, L.. (2009). Phenotypic and genotypic characterization of lactic acid bacteria isolated from Azerbaijani traditional dairy products. in African Journal of Biotechnology, 8(11), 2576-2588.
https://hdl.handle.net/21.15107/rcub_imagine_392

Terzić-Vidojević A, Tolinački M, Živković M, Lozo J, Begović J, Gulahmadov SGO, Kuliev AA, Dalgalarrondo M, Chobert J, Haertle T, Topisirović L. Phenotypic and genotypic characterization of lactic acid bacteria isolated from Azerbaijani traditional dairy products. in African Journal of Biotechnology. 2009;8(11):2576-2588.
https://hdl.handle.net/21.15107/rcub_imagine_392 .

Terzić-Vidojević, Amarela, Tolinački, Maja, Živković, Milica, Lozo, Jelena, Begović, Jelena, Gulahmadov, Sahib Gurban Oglu, Kuliev, Akif Alekperovich, Dalgalarrondo, Michele, Chobert, Jean-Marc, Haertle, Thomas, Topisirović, Ljubiša, "Phenotypic and genotypic characterization of lactic acid bacteria isolated from Azerbaijani traditional dairy products" in African Journal of Biotechnology, 8, no. 11 (2009):2576-2588,
https://hdl.handle.net/21.15107/rcub_imagine_392 .

TY  - JOUR
AU  - Begović, Jelena
AU  - Huys, Geert
AU  - Mayo, Baltasar
AU  - D'Haene, Klaas
AU  - Belen Florez, Ana
AU  - Lozo, Jelena
AU  - Kojić, Milan
AU  - Strahinić, Ivana
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1618
AB  - Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.
PB  - Amsterdam : Elsevier
T2  - Research in Microbiology
T1  - Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance
EP  - 426
IS  - 6
SP  - 421
VL  - 160
DO  - 10.1016/j.resmic.2009.07.005
ER  - 

@article{
author = "Begović, Jelena and Huys, Geert and Mayo, Baltasar and D'Haene, Klaas and Belen Florez, Ana and Lozo, Jelena and Kojić, Milan and Strahinić, Ivana and Topisirović, Ljubiša",
year = "2009",
abstract = "Little is known about the diversity and distribution of resistance determinants in human commensal bacteria. The aim of this study was to determine the molecular mechanism responsible for high-level erythromycin resistance among five human vaginal Lactobacillus rhamnosus isolates. PCR screening for the presence of ermA, ermB and ermC methylase genes revealed no determinants responsible for detected erythromycin resistance. Therefore, sequences of 23S rRNA genes from L. rhamnosus strains were studied by PCR-RFLP analysis and sequencing of 23S rRNA genes. According to the results, in all erythromycin-resistant L. rhamnosus strains, the presence of a A - gt  G transition mutation at position 2058 was discovered. Additionally, the isolates exhibited heterozygosity for the A2058/G2058 mutation among 23S rRNA gene copies. Presumably, the greatest number of mutated 23S rRNA operons was observed for the L. rhamnosus BGHV1' strain that also had the highest MIC for erythromycin (MIC = 2048 mu g mL(-1)). This study reports the presence of transition mutations in the V region of 23S rRNA genes that most probably account for high-level erythromycin resistance observed for the first time in human vaginal lactobacilli.",
publisher = "Amsterdam : Elsevier",
journal = "Research in Microbiology",
title = "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance",
pages = "426-421",
number = "6",
volume = "160",
doi = "10.1016/j.resmic.2009.07.005"
}

Begović, J., Huys, G., Mayo, B., D'Haene, K., Belen Florez, A., Lozo, J., Kojić, M., Strahinić, I.,& Topisirović, L.. (2009). Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology
Amsterdam : Elsevier., 160(6), 421-426.
https://doi.org/10.1016/j.resmic.2009.07.005

Begović J, Huys G, Mayo B, D'Haene K, Belen Florez A, Lozo J, Kojić M, Strahinić I, Topisirović L. Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance. in Research in Microbiology. 2009;160(6):421-426.
doi:10.1016/j.resmic.2009.07.005 .

Begović, Jelena, Huys, Geert, Mayo, Baltasar, D'Haene, Klaas, Belen Florez, Ana, Lozo, Jelena, Kojić, Milan, Strahinić, Ivana, Topisirović, Ljubiša, "Human vaginal Lactobacillus rhamnosus harbor mutation in 23S rRNA associated with erythromycin resistance" in Research in Microbiology, 160, no. 6 (2009):421-426,
https://doi.org/10.1016/j.resmic.2009.07.005 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Fira, Đorđe
AU  - Terzić-Vidojević, Amarela
AU  - Abriouel, Hikmate
AU  - Galvez, Antonio
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/378
AB  - A collection of 26 enterococci isolated from dairy and meat products were tested for antimicrobial and proteolytic activity. Enterococcus faecium and E. faecalis were the most frequent species among tested enterococci, and 11 isolates produced antimicrobial compounds. Results revealed that 10 out of 11 enterococci synthesized enterocins showing antimicrobial activity against food-born pathogen such as Listeria monocytogenes and Staphylococcus aureus. The broadest spectrum of antimicrobial activity was detected in E. faecalis BGPT1-10P and BGPT1-78. E. faecalis BG221 showed antimicrobial activity that was not related to production of enterocin, H2O2 or organic acid. Twenty-five enterococci showed strong or moderate proteolytic activity towards beta-casein. Two isolates, BGPT1-10P and BGPT1-78, showed the most intense hydrolysis of alpha(s1)-, beta-, kappa-casein fractions, total casein as well as gelatin. Extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass of about 29 kDa. Bacteriocin production and proteinase activity of natural isolates of enterococci may be of technological interest in dairy and meat-fermented products.
PB  - Springer, New York
T2  - European Food Research and Technology
T1  - Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products
EP  - 70
IS  - 1
SP  - 63
VL  - 230
DO  - 10.1007/s00217-009-1137-6
ER  - 

@article{
author = "Veljović, Katarina and Fira, Đorđe and Terzić-Vidojević, Amarela and Abriouel, Hikmate and Galvez, Antonio and Topisirović, Ljubiša",
year = "2009",
abstract = "A collection of 26 enterococci isolated from dairy and meat products were tested for antimicrobial and proteolytic activity. Enterococcus faecium and E. faecalis were the most frequent species among tested enterococci, and 11 isolates produced antimicrobial compounds. Results revealed that 10 out of 11 enterococci synthesized enterocins showing antimicrobial activity against food-born pathogen such as Listeria monocytogenes and Staphylococcus aureus. The broadest spectrum of antimicrobial activity was detected in E. faecalis BGPT1-10P and BGPT1-78. E. faecalis BG221 showed antimicrobial activity that was not related to production of enterocin, H2O2 or organic acid. Twenty-five enterococci showed strong or moderate proteolytic activity towards beta-casein. Two isolates, BGPT1-10P and BGPT1-78, showed the most intense hydrolysis of alpha(s1)-, beta-, kappa-casein fractions, total casein as well as gelatin. Extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass of about 29 kDa. Bacteriocin production and proteinase activity of natural isolates of enterococci may be of technological interest in dairy and meat-fermented products.",
publisher = "Springer, New York",
journal = "European Food Research and Technology",
title = "Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products",
pages = "70-63",
number = "1",
volume = "230",
doi = "10.1007/s00217-009-1137-6"
}

Veljović, K., Fira, Đ., Terzić-Vidojević, A., Abriouel, H., Galvez, A.,& Topisirović, L.. (2009). Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products. in European Food Research and Technology
Springer, New York., 230(1), 63-70.
https://doi.org/10.1007/s00217-009-1137-6

Veljović K, Fira Đ, Terzić-Vidojević A, Abriouel H, Galvez A, Topisirović L. Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products. in European Food Research and Technology. 2009;230(1):63-70.
doi:10.1007/s00217-009-1137-6 .

Veljović, Katarina, Fira, Đorđe, Terzić-Vidojević, Amarela, Abriouel, Hikmate, Galvez, Antonio, Topisirović, Ljubiša, "Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products" in European Food Research and Technology, 230, no. 1 (2009):63-70,
https://doi.org/10.1007/s00217-009-1137-6 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Lozo, Jelena
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/397
AB  - U ovom radu su ispitivana dva sira od svežeg kravljeg mleka različitog perioda zrenja. Sirevi su uzeti iz seoskog domaćinstva u regionu Stare Planine, a proizvedeni su bez dodatka starter kulture. Iz oba sira je izolovano ukupno 106 sojeva bakterija mlečne kiseline. Sojevi su testirani klasičnim fiziološkim i API 50 CH testovima. Takođe je ispitivana proteolitička i antimikrobna aktivnost. Identifikacija bakterija mlečne kiseline je rađena rep-PCR analizom sa (GTG)5 prajmerom. Osam vrsta bakterije mlečne kiseline je izolavano iz sira BGPT9 starog četiri dana (Lactobacillus plantarum, Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii, Lactobacillus brevis, Enterococcus faecium, Enterococcus faecalis, Enterococcus durans i Leuconostoc garlicum), dok su u siru BGPT10 starom osam meseci bile prisutne samo dve vrste (Lactobacillus plantarum i Enterococcus faecium). Proteolitičku aktivnost je pokazalo 30 izolata iz sira BGPT9, uglavnom enterokoke. Samo jedan izolat iz sira BGPT10 (koji je pripadao vrsti Lactobacillus plantarum) je posedovao delimičnu sposobnost da hidrolizuje β- kazein. Sedam enterokoka iz sira BGPT9 i četiri enterokoke iz sira BGPT10 je proizvodilo antimikrobne substance.
AB  - In this study two raw cow's milk cheeses of a different ripening period were examined. The cheeses were taken from a country household in the region of mountain Stara Planina and manufactured without adding of starter culture. A total 106 lactic acid bacteria (LAB) strains were isolated from both cheeses. They are tested by classical physiological tests as well as by API 50 CH tests. Proteolytic and antimicrobial activities were done too. Identification of LAB isolates was done by repetitive extragenic palindromic-polimerase chain reaction (rep-PCR) with (GTG)5 primer. The LAB isolates from cheese BGPT9 (four days old) belonged to the eight species of LAB (Lactobacillus plantarum, Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii, Lactobacillus brevis, Enterococcus faecium, Enterococcus faecalis, Enterococcus durans and Leuconostoc garlicum), while in the BGPT10 cheese (eight months old) only two species were present (Lactobacillus plantarum and Enterococcus faecium). Proteolytic activity showed 30 LAB from BGPT9 cheese, mainly enterococci. From BGPT10 cheese only one isolate (which belonged to the Lactobacillus plantarum species) possessed partial ability to hydrolyze β-casein. Seven enterococci from BGPT9 cheese and four enterococci from BGPT10 cheese produced antimicrobial compounds.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Dominantne bakterije mlečne kiseline u pirotskim sirevima različitog perioda zrenja proizvedenim u domaćinstvu
T1  - Dominant lactic acid bacteria in artisanal Pirot cheeses of different ripening period
EP  - 352
IS  - 3
SP  - 339
VL  - 41
DO  - 10.2298/GENSR0903341T
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Lozo, Jelena and Topisirović, Ljubiša",
year = "2009",
abstract = "U ovom radu su ispitivana dva sira od svežeg kravljeg mleka različitog perioda zrenja. Sirevi su uzeti iz seoskog domaćinstva u regionu Stare Planine, a proizvedeni su bez dodatka starter kulture. Iz oba sira je izolovano ukupno 106 sojeva bakterija mlečne kiseline. Sojevi su testirani klasičnim fiziološkim i API 50 CH testovima. Takođe je ispitivana proteolitička i antimikrobna aktivnost. Identifikacija bakterija mlečne kiseline je rađena rep-PCR analizom sa (GTG)5 prajmerom. Osam vrsta bakterije mlečne kiseline je izolavano iz sira BGPT9 starog četiri dana (Lactobacillus plantarum, Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii, Lactobacillus brevis, Enterococcus faecium, Enterococcus faecalis, Enterococcus durans i Leuconostoc garlicum), dok su u siru BGPT10 starom osam meseci bile prisutne samo dve vrste (Lactobacillus plantarum i Enterococcus faecium). Proteolitičku aktivnost je pokazalo 30 izolata iz sira BGPT9, uglavnom enterokoke. Samo jedan izolat iz sira BGPT10 (koji je pripadao vrsti Lactobacillus plantarum) je posedovao delimičnu sposobnost da hidrolizuje β- kazein. Sedam enterokoka iz sira BGPT9 i četiri enterokoke iz sira BGPT10 je proizvodilo antimikrobne substance., In this study two raw cow's milk cheeses of a different ripening period were examined. The cheeses were taken from a country household in the region of mountain Stara Planina and manufactured without adding of starter culture. A total 106 lactic acid bacteria (LAB) strains were isolated from both cheeses. They are tested by classical physiological tests as well as by API 50 CH tests. Proteolytic and antimicrobial activities were done too. Identification of LAB isolates was done by repetitive extragenic palindromic-polimerase chain reaction (rep-PCR) with (GTG)5 primer. The LAB isolates from cheese BGPT9 (four days old) belonged to the eight species of LAB (Lactobacillus plantarum, Lactobacillus paracasei subsp. paracasei, Lactobacillus delbrueckii, Lactobacillus brevis, Enterococcus faecium, Enterococcus faecalis, Enterococcus durans and Leuconostoc garlicum), while in the BGPT10 cheese (eight months old) only two species were present (Lactobacillus plantarum and Enterococcus faecium). Proteolytic activity showed 30 LAB from BGPT9 cheese, mainly enterococci. From BGPT10 cheese only one isolate (which belonged to the Lactobacillus plantarum species) possessed partial ability to hydrolyze β-casein. Seven enterococci from BGPT9 cheese and four enterococci from BGPT10 cheese produced antimicrobial compounds.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Dominantne bakterije mlečne kiseline u pirotskim sirevima različitog perioda zrenja proizvedenim u domaćinstvu, Dominant lactic acid bacteria in artisanal Pirot cheeses of different ripening period",
pages = "352-339",
number = "3",
volume = "41",
doi = "10.2298/GENSR0903341T"
}

Terzić-Vidojević, A., Lozo, J.,& Topisirović, L.. (2009). Dominantne bakterije mlečne kiseline u pirotskim sirevima različitog perioda zrenja proizvedenim u domaćinstvu. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 41(3), 339-352.
https://doi.org/10.2298/GENSR0903341T

Terzić-Vidojević A, Lozo J, Topisirović L. Dominantne bakterije mlečne kiseline u pirotskim sirevima različitog perioda zrenja proizvedenim u domaćinstvu. in Genetika-Belgrade. 2009;41(3):339-352.
doi:10.2298/GENSR0903341T .

Terzić-Vidojević, Amarela, Lozo, Jelena, Topisirović, Ljubiša, "Dominantne bakterije mlečne kiseline u pirotskim sirevima različitog perioda zrenja proizvedenim u domaćinstvu" in Genetika-Belgrade, 41, no. 3 (2009):339-352,
https://doi.org/10.2298/GENSR0903341T . .

TY  - JOUR
AU  - Živković, Milica
AU  - Tolinački, Maja
AU  - Fira, Đorđe
AU  - Golić, Nataša
AU  - Topisirović, Ljubiša
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/346
AB  - Comparison of cell-wall-bound extracellular proteinases (CEPs) from Lactobacillus paracasei (LBP) ssp. paracasei natural isolates BGHN14, BGAR75 and BGAR76 with Lactococcus lactis (LCL) ssp. cremoris Wg2, in their action on alpha(S1)-, beta- and kappa-casein was done. The CEPs of LBP strains were able to degrade alpha(S1)- and beta-caseins and their caseinolytic specificity depended on the type of buffer used. These CEPs, compared with LCL Wg2, differ in four amino acid residues in small segments predicted to be involved in substrate binding. The most striking features of this comparison are the presence of Ala instead of Ser(329) and the presence of Thr instead of Asn(256) and Ala(299), in the subtilisin-like region of the CEP in LBP natural isolates. Additional conservative amino acid substitution Leu to Ile(364) was found.
PB  - Springer, Dordrecht
T2  - Folia Microbiologica
T1  - Variation in specificity of the PrtP extracellular proteinases in Lactococcus lactis and Lactobacillus paracasei subsp paracasei
EP  - 194
IS  - 3
SP  - 188
VL  - 54
DO  - 10.1007/s12223-009-0029-2
ER  - 

@article{
author = "Živković, Milica and Tolinački, Maja and Fira, Đorđe and Golić, Nataša and Topisirović, Ljubiša",
year = "2009",
abstract = "Comparison of cell-wall-bound extracellular proteinases (CEPs) from Lactobacillus paracasei (LBP) ssp. paracasei natural isolates BGHN14, BGAR75 and BGAR76 with Lactococcus lactis (LCL) ssp. cremoris Wg2, in their action on alpha(S1)-, beta- and kappa-casein was done. The CEPs of LBP strains were able to degrade alpha(S1)- and beta-caseins and their caseinolytic specificity depended on the type of buffer used. These CEPs, compared with LCL Wg2, differ in four amino acid residues in small segments predicted to be involved in substrate binding. The most striking features of this comparison are the presence of Ala instead of Ser(329) and the presence of Thr instead of Asn(256) and Ala(299), in the subtilisin-like region of the CEP in LBP natural isolates. Additional conservative amino acid substitution Leu to Ile(364) was found.",
publisher = "Springer, Dordrecht",
journal = "Folia Microbiologica",
title = "Variation in specificity of the PrtP extracellular proteinases in Lactococcus lactis and Lactobacillus paracasei subsp paracasei",
pages = "194-188",
number = "3",
volume = "54",
doi = "10.1007/s12223-009-0029-2"
}

Živković, M., Tolinački, M., Fira, Đ., Golić, N.,& Topisirović, L.. (2009). Variation in specificity of the PrtP extracellular proteinases in Lactococcus lactis and Lactobacillus paracasei subsp paracasei. in Folia Microbiologica
Springer, Dordrecht., 54(3), 188-194.
https://doi.org/10.1007/s12223-009-0029-2

Živković M, Tolinački M, Fira Đ, Golić N, Topisirović L. Variation in specificity of the PrtP extracellular proteinases in Lactococcus lactis and Lactobacillus paracasei subsp paracasei. in Folia Microbiologica. 2009;54(3):188-194.
doi:10.1007/s12223-009-0029-2 .

Živković, Milica, Tolinački, Maja, Fira, Đorđe, Golić, Nataša, Topisirović, Ljubiša, "Variation in specificity of the PrtP extracellular proteinases in Lactococcus lactis and Lactobacillus paracasei subsp paracasei" in Folia Microbiologica, 54, no. 3 (2009):188-194,
https://doi.org/10.1007/s12223-009-0029-2 . .