TY  - JOUR
AU  - Pantelić, Brana
AU  - Siaperas, Romanos
AU  - Budin, Clémence
AU  - de Boer, Tjalf
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14445
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2337
AB  - Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.
PB  - Wiley
T2  - Microbial Biotechnology
T2  - Microbial Biotechnology
T1  - Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production
IS  - 3
SP  - e14445
VL  - 17
DO  - 10.1111/1751-7915.14445
ER  - 

@article{
author = "Pantelić, Brana and Siaperas, Romanos and Budin, Clémence and de Boer, Tjalf and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.",
publisher = "Wiley",
journal = "Microbial Biotechnology, Microbial Biotechnology",
title = "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production",
number = "3",
pages = "e14445",
volume = "17",
doi = "10.1111/1751-7915.14445"
}

Pantelić, B., Siaperas, R., Budin, C., de Boer, T., Topakas, E.,& Nikodinović-Runić, J.. (2024). Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology
Wiley., 17(3), e14445.
https://doi.org/10.1111/1751-7915.14445

Pantelić B, Siaperas R, Budin C, de Boer T, Topakas E, Nikodinović-Runić J. Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology. 2024;17(3):e14445.
doi:10.1111/1751-7915.14445 .

Pantelić, Brana, Siaperas, Romanos, Budin, Clémence, de Boer, Tjalf, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production" in Microbial Biotechnology, 17, no. 3 (2024):e14445,
https://doi.org/10.1111/1751-7915.14445 . .

TY  - JOUR
AU  - Lazić, Jelena
AU  - Filipović, Vuk
AU  - Pantelić, Lena
AU  - Milovanović, Jelena
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2024.1351583
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2387
AB  - Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.
PB  - Frontiers
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Late-stage diversification of bacterial natural products through biocatalysis
VL  - 12
DO  - 10.3389/fbioe.2024.1351583
ER  - 

@article{
author = "Lazić, Jelena and Filipović, Vuk and Pantelić, Lena and Milovanović, Jelena and Vojnović, Sandra and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.",
publisher = "Frontiers",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Late-stage diversification of bacterial natural products through biocatalysis",
volume = "12",
doi = "10.3389/fbioe.2024.1351583"
}

Lazić, J., Filipović, V., Pantelić, L., Milovanović, J., Vojnović, S.,& Nikodinović-Runić, J.. (2024). Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology
Frontiers., 12.
https://doi.org/10.3389/fbioe.2024.1351583

Lazić J, Filipović V, Pantelić L, Milovanović J, Vojnović S, Nikodinović-Runić J. Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology. 2024;12.
doi:10.3389/fbioe.2024.1351583 .

Lazić, Jelena, Filipović, Vuk, Pantelić, Lena, Milovanović, Jelena, Vojnović, Sandra, Nikodinović-Runić, Jasmina, "Late-stage diversification of bacterial natural products through biocatalysis" in Frontiers in Bioengineering and Biotechnology, 12 (2024),
https://doi.org/10.3389/fbioe.2024.1351583 . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Cosović, Vladan
AU  - Atanase, Leonard Ionut
AU  - Freitas, Filomena
AU  - Matos, Mariana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1714
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
IS  - 3
SP  - 1906
VL  - 24
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Cosović, Vladan and Atanase, Leonard Ionut and Freitas, Filomena and Matos, Mariana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
number = "3",
pages = "1906",
volume = "24",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Cosović, V., Atanase, L. I., Freitas, F., Matos, M.,& Nikodinović-Runić, J.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences, 24(3), 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Cosović V, Atanase LI, Freitas F, Matos M, Nikodinović-Runić J. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24(3):1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Cosović, Vladan, Atanase, Leonard Ionut, Freitas, Filomena, Matos, Mariana, Nikodinović-Runić, Jasmina, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24, no. 3 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - CONF
AU  - Lazić, Jelena
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.chem.bg.ac.rs/pz/news1.py?q=3820&l=1
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2205
AB  - Microorganisms, our planet’s original inhabitants discovered with the invention of the
first microscope in the 17th century, have consistently facilitated our daily life. However,
our modern life generates enormous amounts of wastes, such as plastic, food, and
chemicals from the pharmaceutical industry. Bacterial natural products hold an
important position in this industry, as drug leads in synthetic chemistry and biology,
essential for the discovery of effective agents against a variety of human diseases. If the
existing waste is used as a nutrient source for microbial production of valuable
biomolecules, that concept is called “waste to value” or “upcycling”.
This concept was explored using bacterial biopigment prodigiosin (PG, Fig. 1) as part of
the BioECOLogics project. This proof of concept demonstrates how the bacteria Serratia
marcescens ATCC 27117 can use a waste stream from the food industry as a carbon
source to grow and produce its bioactive secondary metabolite PG. The unique structure
of this molecule was changed through green chemical [1] and biopolymer formulation
[2] approaches, as well as metal complexation. Finally, these sustainable biotherapeutics
were validated in vitro (antimicrobial, anticancer) and in vivo (nematode Caenorhabditis
elegans and zebrafish Danio rerio).
PB  - Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia
T1  - From waste streams to biotherapeutics: making a connection using bacteria
EP  - 6
SP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2205
ER  - 

@conference{
author = "Lazić, Jelena and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Microorganisms, our planet’s original inhabitants discovered with the invention of the
first microscope in the 17th century, have consistently facilitated our daily life. However,
our modern life generates enormous amounts of wastes, such as plastic, food, and
chemicals from the pharmaceutical industry. Bacterial natural products hold an
important position in this industry, as drug leads in synthetic chemistry and biology,
essential for the discovery of effective agents against a variety of human diseases. If the
existing waste is used as a nutrient source for microbial production of valuable
biomolecules, that concept is called “waste to value” or “upcycling”.
This concept was explored using bacterial biopigment prodigiosin (PG, Fig. 1) as part of
the BioECOLogics project. This proof of concept demonstrates how the bacteria Serratia
marcescens ATCC 27117 can use a waste stream from the food industry as a carbon
source to grow and produce its bioactive secondary metabolite PG. The unique structure
of this molecule was changed through green chemical [1] and biopolymer formulation
[2] approaches, as well as metal complexation. Finally, these sustainable biotherapeutics
were validated in vitro (antimicrobial, anticancer) and in vivo (nematode Caenorhabditis
elegans and zebrafish Danio rerio).",
publisher = "Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia",
title = "From waste streams to biotherapeutics: making a connection using bacteria",
pages = "6-6",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2205"
}

Lazić, J.,& Nikodinović-Runić, J.. (2023). From waste streams to biotherapeutics: making a connection using bacteria. in 9th Conference of Young Chemists of Serbia
Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club., 6-6.
https://hdl.handle.net/21.15107/rcub_imagine_2205

Lazić J, Nikodinović-Runić J. From waste streams to biotherapeutics: making a connection using bacteria. in 9th Conference of Young Chemists of Serbia. 2023;:6-6.
https://hdl.handle.net/21.15107/rcub_imagine_2205 .

Lazić, Jelena, Nikodinović-Runić, Jasmina, "From waste streams to biotherapeutics: making a connection using bacteria" in 9th Conference of Young Chemists of Serbia (2023):6-6,
https://hdl.handle.net/21.15107/rcub_imagine_2205 .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Nenadović, Marija
AU  - Pantelić, Brana
AU  - Ponjavić,  Marijana
AU  - Sourkouni, Georgia
AU  - Kalogirou, Charalampia
AU  - Argirusis, Christos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://esabweb.org/E_CONGRESS/Poster+Programme/Day/Online/All+day/Enhanced+enzymatic+depolymerization+of+polylactic+acid+%28PLA%29+through+plasma+pretreatment+and+subsequent+conversion+to+biopolymer.html
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2232
AB  - Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.
PB  - European Society of Applied Biocatalysis
C3  - ESAB E-Congress
T1  - Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2232
ER  - 

@conference{
author = "Milovanović, Jelena and Nenadović, Marija and Pantelić, Brana and Ponjavić,  Marijana and Sourkouni, Georgia and Kalogirou, Charalampia and Argirusis, Christos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.",
publisher = "European Society of Applied Biocatalysis",
journal = "ESAB E-Congress",
title = "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2232"
}

Milovanović, J., Nenadović, M., Pantelić, B., Ponjavić, M., Sourkouni, G., Kalogirou, C., Argirusis, C.,& Nikodinović-Runić, J.. (2023). Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress
European Society of Applied Biocatalysis..
https://hdl.handle.net/21.15107/rcub_imagine_2232

Milovanović J, Nenadović M, Pantelić B, Ponjavić M, Sourkouni G, Kalogirou C, Argirusis C, Nikodinović-Runić J. Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

Milovanović, Jelena, Nenadović, Marija, Pantelić, Brana, Ponjavić,  Marijana, Sourkouni, Georgia, Kalogirou, Charalampia, Argirusis, Christos, Nikodinović-Runić, Jasmina, "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer" in ESAB E-Congress (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

TY  - CONF
AU  - Andrejević, Tina
AU  - Ašanin, Darko
AU  - Pantelić, Lena
AU  - Pantović, Bojana
AU  - Nikodinović-Runić, Jasmina
AU  - Glišić, Biljana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2293
AB  - Pyocyanin (PYO) is a green blue pigment that is produced extracellularly by the Gram- negative bacteria Pseudomonas aeruginosa. Its color depends on pH value. It exists in blue zwitterion form at neutral and alkaline conditions, while in an acidic environment, it becomes pink after protonation. PYO has shown the antibacterial activity, as well as the ability to inhibit the growth of fungi like Aspergillus fumigatus and Candida albicans. Moreover, it shows the high cytotoxic effect against the human pancreatic cancer cells by inducing their apoptosis. To evaluate the possible mechanism of antimicrobial activity of PYO, in the present study, we have investigated its interactions with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy. The obtained value of binding constant to BSA is relatively high (KA = 5.3 × 10^6 M^-1 s^-1), showing the ability of PYO to bind to this transport protein. We have also used synchronous fluorescence spectroscopy to explore the structural changes in BSA in the presence of the studied biopigment. In contrast with the mentioned results for binding to BSA, PYO has shown a low affinity to ct-DNA, what can be seen from the value of its binding constant (KA = 7.8 × 10^3 M^-1 s^-1).
PB  - MDPI
C3  - Medical Sciences Forum
T1  - DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa
VL  - n/a
DO  - 10.3390/ECMC2023-15654
ER  - 

@conference{
author = "Andrejević, Tina and Ašanin, Darko and Pantelić, Lena and Pantović, Bojana and Nikodinović-Runić, Jasmina and Glišić, Biljana",
year = "2023",
abstract = "Pyocyanin (PYO) is a green blue pigment that is produced extracellularly by the Gram- negative bacteria Pseudomonas aeruginosa. Its color depends on pH value. It exists in blue zwitterion form at neutral and alkaline conditions, while in an acidic environment, it becomes pink after protonation. PYO has shown the antibacterial activity, as well as the ability to inhibit the growth of fungi like Aspergillus fumigatus and Candida albicans. Moreover, it shows the high cytotoxic effect against the human pancreatic cancer cells by inducing their apoptosis. To evaluate the possible mechanism of antimicrobial activity of PYO, in the present study, we have investigated its interactions with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission spectroscopy. The obtained value of binding constant to BSA is relatively high (KA = 5.3 × 10^6 M^-1 s^-1), showing the ability of PYO to bind to this transport protein. We have also used synchronous fluorescence spectroscopy to explore the structural changes in BSA in the presence of the studied biopigment. In contrast with the mentioned results for binding to BSA, PYO has shown a low affinity to ct-DNA, what can be seen from the value of its binding constant (KA = 7.8 × 10^3 M^-1 s^-1).",
publisher = "MDPI",
journal = "Medical Sciences Forum",
title = "DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa",
volume = "n/a",
doi = "10.3390/ECMC2023-15654"
}

Andrejević, T., Ašanin, D., Pantelić, L., Pantović, B., Nikodinović-Runić, J.,& Glišić, B.. (2023). DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa. in Medical Sciences Forum
MDPI., n/a.
https://doi.org/10.3390/ECMC2023-15654

Andrejević T, Ašanin D, Pantelić L, Pantović B, Nikodinović-Runić J, Glišić B. DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa. in Medical Sciences Forum. 2023;n/a.
doi:10.3390/ECMC2023-15654 .

Andrejević, Tina, Ašanin, Darko, Pantelić, Lena, Pantović, Bojana, Nikodinović-Runić, Jasmina, Glišić, Biljana, "DNA/BSA binding affinity of pyocyanin produced by Pseudomonas aeruginosa" in Medical Sciences Forum, n/a (2023),
https://doi.org/10.3390/ECMC2023-15654 . .

TY  - CONF
AU  - Glišić, Biljana
AU  - Andrejević, Tina
AU  - Lazić, Jelena
AU  - Ilić-Tomić, Tatjana
AU  - Ašanin, Darko
AU  - Pantović, Bojana
AU  - Djuran, Miloš
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://isabc2023.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1926
AB  - Prodigiosin (PG) is a red biopigment produced as a secondary metabolite by
microorganisms such as Serratia marcescens and Streptomyces. In recent years, this tripyrrole
compound has attracted an increasing attention due to its antibacterial, antimalarial, and
immunosuppressive activities [1]. It is also known for its antitumor activity, inducing the cell
death by apoptosis in different human cancer cell lines [2]. Considering this, in the present
study, we investigated the interactions of prodigiosin and its copper(II) complex (CuPG; the
structural formula is presented below), whose crystal structure was determined previously [2],
with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission
spectroscopy to clarify their binding affinities toward these biomolecules. The antimicrobial
activity of the synthesized CuPG complex and PG ligand was evaluated in vitro against various
microorganisms that can lead to many infections. Moreover, CuPG and PG were evaluated in
a cell viability assay on a healthy MRC-5 cell line, as well as a panel of MDA-MB-231, A549,
A375, and HCT116 cancer cell lines.
PB  - Greece : University of Ioannina
C3  - 16th International Symposium on Applied Bioinorganic Chemistry
T1  - DNA/BSA interactions and biological activity of prodigiosin and its
copper(II) complex
SP  - 264
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1926
ER  - 

@conference{
author = "Glišić, Biljana and Andrejević, Tina and Lazić, Jelena and Ilić-Tomić, Tatjana and Ašanin, Darko and Pantović, Bojana and Djuran, Miloš and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Prodigiosin (PG) is a red biopigment produced as a secondary metabolite by
microorganisms such as Serratia marcescens and Streptomyces. In recent years, this tripyrrole
compound has attracted an increasing attention due to its antibacterial, antimalarial, and
immunosuppressive activities [1]. It is also known for its antitumor activity, inducing the cell
death by apoptosis in different human cancer cell lines [2]. Considering this, in the present
study, we investigated the interactions of prodigiosin and its copper(II) complex (CuPG; the
structural formula is presented below), whose crystal structure was determined previously [2],
with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) by fluorescence emission
spectroscopy to clarify their binding affinities toward these biomolecules. The antimicrobial
activity of the synthesized CuPG complex and PG ligand was evaluated in vitro against various
microorganisms that can lead to many infections. Moreover, CuPG and PG were evaluated in
a cell viability assay on a healthy MRC-5 cell line, as well as a panel of MDA-MB-231, A549,
A375, and HCT116 cancer cell lines.",
publisher = "Greece : University of Ioannina",
journal = "16th International Symposium on Applied Bioinorganic Chemistry",
title = "DNA/BSA interactions and biological activity of prodigiosin and its
copper(II) complex",
pages = "264",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1926"
}

Glišić, B., Andrejević, T., Lazić, J., Ilić-Tomić, T., Ašanin, D., Pantović, B., Djuran, M.,& Nikodinović-Runić, J.. (2023). DNA/BSA interactions and biological activity of prodigiosin and its
copper(II) complex. in 16th International Symposium on Applied Bioinorganic Chemistry
Greece : University of Ioannina., 264.
https://hdl.handle.net/21.15107/rcub_imagine_1926

Glišić B, Andrejević T, Lazić J, Ilić-Tomić T, Ašanin D, Pantović B, Djuran M, Nikodinović-Runić J. DNA/BSA interactions and biological activity of prodigiosin and its
copper(II) complex. in 16th International Symposium on Applied Bioinorganic Chemistry. 2023;:264.
https://hdl.handle.net/21.15107/rcub_imagine_1926 .

Glišić, Biljana, Andrejević, Tina, Lazić, Jelena, Ilić-Tomić, Tatjana, Ašanin, Darko, Pantović, Bojana, Djuran, Miloš, Nikodinović-Runić, Jasmina, "DNA/BSA interactions and biological activity of prodigiosin and its
copper(II) complex" in 16th International Symposium on Applied Bioinorganic Chemistry (2023):264,
https://hdl.handle.net/21.15107/rcub_imagine_1926 .

TY  - CONF
AU  - Lazić, Jelena
AU  - Milovanović, Jelena
AU  - Anejević, Tina
AU  - Ašanin, Darko
AU  - Ilić-Tomić, Tatjana
AU  - Glišić, Biljana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2235
AB  - Prodigiosin (PG, Fig. 1a) is a biologically active
pyrrolylpyrromethene alkaloid whose structure was
first confirmed in 1962 [1]. PG is commonly
produced by Gram-negative bacteria, such as
Serratia spp. and has an eco-physiological role [2].
Its biological activities were extensively researched,
and numerous pharmacological properties were
established, including anticancer and
immunosuppressive However, high cost of extraction and purification
still represent the bottleneck in the microbial
production of PG. Meat and fish processing wastes have high potential as raw materials for conversion
into useful products of higher value. In this study,
meat offcuts were assessed as the sole nutrient for
the fermentative production of PG from S.
marcescens. Using this substrate lowered the
cultivation medium cost and shortened the
fermentation time to 12 h, while allowing a
satisfying PG yield of 83.1 mg/L. The isolated PG
was used in one-step reactions with CuCl₂ or ZnCl₂
in terc-BuOH at 25 °C. The obtained [Cu(PG)Cl] (Fig.
1b) and [Zn(PG)₂] (Fig. 1c) complexes were
characterized by UV-Vis and IR spectroscopy and
their bioactivity potential was assessed.
Antimicrobial activity was assessed in a disc assay
against 4 human pathogens: Escherichia coli NCTC
9001, Pseudomonas aeruginosa ATCC 10332,
Staphylococcus aureus NCTC 6571, Candida
albicans ATCC 10231, but no effect was observed
for the tested concentrations of 200 μg per disc and
lower. However, the anticancer potential of the new
derivatives is promising and the bovine serum
albumin (BSA) binding study revealed that
complexes bind to BSA tightly and reversibly [4].
C3  - 10th Conference of Mikrobiokosmos
T1  - Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2235
ER  - 

@conference{
author = "Lazić, Jelena and Milovanović, Jelena and Anejević, Tina and Ašanin, Darko and Ilić-Tomić, Tatjana and Glišić, Biljana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Prodigiosin (PG, Fig. 1a) is a biologically active
pyrrolylpyrromethene alkaloid whose structure was
first confirmed in 1962 [1]. PG is commonly
produced by Gram-negative bacteria, such as
Serratia spp. and has an eco-physiological role [2].
Its biological activities were extensively researched,
and numerous pharmacological properties were
established, including anticancer and
immunosuppressive However, high cost of extraction and purification
still represent the bottleneck in the microbial
production of PG. Meat and fish processing wastes have high potential as raw materials for conversion
into useful products of higher value. In this study,
meat offcuts were assessed as the sole nutrient for
the fermentative production of PG from S.
marcescens. Using this substrate lowered the
cultivation medium cost and shortened the
fermentation time to 12 h, while allowing a
satisfying PG yield of 83.1 mg/L. The isolated PG
was used in one-step reactions with CuCl₂ or ZnCl₂
in terc-BuOH at 25 °C. The obtained [Cu(PG)Cl] (Fig.
1b) and [Zn(PG)₂] (Fig. 1c) complexes were
characterized by UV-Vis and IR spectroscopy and
their bioactivity potential was assessed.
Antimicrobial activity was assessed in a disc assay
against 4 human pathogens: Escherichia coli NCTC
9001, Pseudomonas aeruginosa ATCC 10332,
Staphylococcus aureus NCTC 6571, Candida
albicans ATCC 10231, but no effect was observed
for the tested concentrations of 200 μg per disc and
lower. However, the anticancer potential of the new
derivatives is promising and the bovine serum
albumin (BSA) binding study revealed that
complexes bind to BSA tightly and reversibly [4].",
journal = "10th Conference of Mikrobiokosmos",
title = "Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2235"
}

Lazić, J., Milovanović, J., Anejević, T., Ašanin, D., Ilić-Tomić, T., Glišić, B.,& Nikodinović-Runić, J.. (2023). Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II). in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2235

Lazić J, Milovanović J, Anejević T, Ašanin D, Ilić-Tomić T, Glišić B, Nikodinović-Runić J. Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II). in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2235 .

Lazić, Jelena, Milovanović, Jelena, Anejević, Tina, Ašanin, Darko, Ilić-Tomić, Tatjana, Glišić, Biljana, Nikodinović-Runić, Jasmina, "Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2235 .

TY  - CONF
AU  - Pantelić, Lena
AU  - Lazić, Jelena
AU  - Milivojević, Dušan
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1917
AB  - Industrialization, as well as improper waste management, has led to the accumulation
of a large amount of kitchen and food waste, making it omnipresent in every corner of the
world [1]. This waste stream is considered to be a significant portion of the total
biodegradable waste and a major contributor to greenhouse gases induced pollution [2].
According to the Food and Agriculture Organization official data, an astonishing 1.3 billion
tons of food is wasted annually, emphasizing the need to establish multiple biorefining
strategies in order to minimize waste pollution, whilst producing different valuable products
in a sustainable process [3].
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Food waste utilization in the production of pyocyanin, a valuable bacterial biopigment
EP  - 172
SP  - 171
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1917
ER  - 

@conference{
author = "Pantelić, Lena and Lazić, Jelena and Milivojević, Dušan and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Industrialization, as well as improper waste management, has led to the accumulation
of a large amount of kitchen and food waste, making it omnipresent in every corner of the
world [1]. This waste stream is considered to be a significant portion of the total
biodegradable waste and a major contributor to greenhouse gases induced pollution [2].
According to the Food and Agriculture Organization official data, an astonishing 1.3 billion
tons of food is wasted annually, emphasizing the need to establish multiple biorefining
strategies in order to minimize waste pollution, whilst producing different valuable products
in a sustainable process [3].",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Food waste utilization in the production of pyocyanin, a valuable bacterial biopigment",
pages = "172-171",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1917"
}

Pantelić, L., Lazić, J., Milivojević, D.,& Nikodinović-Runić, J.. (2023). Food waste utilization in the production of pyocyanin, a valuable bacterial biopigment. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 171-172.
https://hdl.handle.net/21.15107/rcub_imagine_1917

Pantelić L, Lazić J, Milivojević D, Nikodinović-Runić J. Food waste utilization in the production of pyocyanin, a valuable bacterial biopigment. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:171-172.
https://hdl.handle.net/21.15107/rcub_imagine_1917 .

Pantelić, Lena, Lazić, Jelena, Milivojević, Dušan, Nikodinović-Runić, Jasmina, "Food waste utilization in the production of pyocyanin, a valuable bacterial biopigment" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):171-172,
https://hdl.handle.net/21.15107/rcub_imagine_1917 .

TY  - CONF
AU  - Pantelić, Lena
AU  - Lazić, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://www.accelevents.com/e/circular-bioeconomy-2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1923
AB  - Potato industry is one of the major food industries that generates considerable amounts of
potato peels and wastewater (1). These wastes can then be utilized as components of
microbial media in biotechnological production of pyocyanin (PYO) and 1-hydroxyphenazine
(1-HP) using Pseudomonas aeruginosa (2). PYO and 1-HP possess important biological
activities, thus could be applied in the field of medicine, and can be used as biocontrolling
agents (2). However, their application is hindered due to high costs associated with their large
scale production.
In this work, we established a fermentation process which utilizes either potato peels or
potato wastewater as the sole nutrient source to obtain PYO and 1-HP. P. aeruginosa BK25H
strain was selected from our in-house collection. This approach afforded 10 mg/l PYO and
9 mg/l 1-HP using potato wastewater and 15 mg/l PYO and 11 g/l 1-HP using potato peels
after 24 h incubation. This work is the step towards zero-pollution and conversion of waste
to valuable microbial products.
C3  - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming
T1  - A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1923
ER  - 

@conference{
author = "Pantelić, Lena and Lazić, Jelena and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2023",
abstract = "Potato industry is one of the major food industries that generates considerable amounts of
potato peels and wastewater (1). These wastes can then be utilized as components of
microbial media in biotechnological production of pyocyanin (PYO) and 1-hydroxyphenazine
(1-HP) using Pseudomonas aeruginosa (2). PYO and 1-HP possess important biological
activities, thus could be applied in the field of medicine, and can be used as biocontrolling
agents (2). However, their application is hindered due to high costs associated with their large
scale production.
In this work, we established a fermentation process which utilizes either potato peels or
potato wastewater as the sole nutrient source to obtain PYO and 1-HP. P. aeruginosa BK25H
strain was selected from our in-house collection. This approach afforded 10 mg/l PYO and
9 mg/l 1-HP using potato wastewater and 15 mg/l PYO and 11 g/l 1-HP using potato peels
after 24 h incubation. This work is the step towards zero-pollution and conversion of waste
to valuable microbial products.",
journal = "Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming",
title = "A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1923"
}

Pantelić, L., Lazić, J., Nikodinović-Runić, J.,& Vojnović, S.. (2023). A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 53.
https://hdl.handle.net/21.15107/rcub_imagine_1923

Pantelić L, Lazić J, Nikodinović-Runić J, Vojnović S. A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming. 2023;:53.
https://hdl.handle.net/21.15107/rcub_imagine_1923 .

Pantelić, Lena, Lazić, Jelena, Nikodinović-Runić, Jasmina, Vojnović, Sandra, "A biotechnological process for the production of pyocyanin and 1-hydroxyphenazine using waste streams from the potato chips industry" in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming (2023):53,
https://hdl.handle.net/21.15107/rcub_imagine_1923 .

TY  - JOUR
AU  - Pantelić, Lena
AU  - Bogojević Škaro, Sanja
AU  - Vojnović, Sandra
AU  - Oliveira, Rui
AU  - Lazić, Jelena
AU  - Ilić-Tomić, Tatjana
AU  - Milivojević, Dušan
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0141022923001308
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2078
AB  - Phenazines, including pyocyanin (PYO) and 1-hydroxyphenazine (1-HP) are extracellular secondary metabolites and multifunctional pigments of Pseudomonas aeruginosa responsible for its blue-green color. These versatile molecules are electrochemically active, involved in significant biological activities giving fitness to the host, but also recognized as antimicrobial and anticancer agents. Their wider application is still limited partly due to the cost of carbon substrate for production, which can be solved by the utilization of carbon from food waste within the biorefinery concept. In this study, a variety of food waste streams (banana peel, potato peel, potato washing, stale bread, yoghurt, processed meat, boiled eggs and mixed canteen waste) was used as sole nutrient source in submerged cultures of P. aeruginosa BK25H. Stale bread was identified as the most suitable substrate to support phenazine biopigments production and bacterial growth. This was further increased in 5-liter fermenter when on average 5.2mgL-1 of PYO and 4.4mgL-1 of 1-HP were purified after 24h batch cultivations from the fermentation medium consisting of homogenized stale bread in tap water. Purified biopigments showed moderate antimicrobial activity, and showed different toxicity profiles, with PYO not being toxic against Caenorhabditis elegans, a free-living soil nematode up to 300µgmL-1 and 1-HP showing lethal effects at 75µgmL-1. Therefore, stale bread waste stream with minimal pretreatment should be considered as suitable biorefinery feedstock, as it can support the production of valuable biopigments such as phenazines.
T2  - Enzyme and Microbial Technology
T1  - Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine
SP  - 110322
VL  - 171
DO  - 10.1016/j.enzmictec.2023.110322
ER  - 

@article{
author = "Pantelić, Lena and Bogojević Škaro, Sanja and Vojnović, Sandra and Oliveira, Rui and Lazić, Jelena and Ilić-Tomić, Tatjana and Milivojević, Dušan and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Phenazines, including pyocyanin (PYO) and 1-hydroxyphenazine (1-HP) are extracellular secondary metabolites and multifunctional pigments of Pseudomonas aeruginosa responsible for its blue-green color. These versatile molecules are electrochemically active, involved in significant biological activities giving fitness to the host, but also recognized as antimicrobial and anticancer agents. Their wider application is still limited partly due to the cost of carbon substrate for production, which can be solved by the utilization of carbon from food waste within the biorefinery concept. In this study, a variety of food waste streams (banana peel, potato peel, potato washing, stale bread, yoghurt, processed meat, boiled eggs and mixed canteen waste) was used as sole nutrient source in submerged cultures of P. aeruginosa BK25H. Stale bread was identified as the most suitable substrate to support phenazine biopigments production and bacterial growth. This was further increased in 5-liter fermenter when on average 5.2mgL-1 of PYO and 4.4mgL-1 of 1-HP were purified after 24h batch cultivations from the fermentation medium consisting of homogenized stale bread in tap water. Purified biopigments showed moderate antimicrobial activity, and showed different toxicity profiles, with PYO not being toxic against Caenorhabditis elegans, a free-living soil nematode up to 300µgmL-1 and 1-HP showing lethal effects at 75µgmL-1. Therefore, stale bread waste stream with minimal pretreatment should be considered as suitable biorefinery feedstock, as it can support the production of valuable biopigments such as phenazines.",
journal = "Enzyme and Microbial Technology",
title = "Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine",
pages = "110322",
volume = "171",
doi = "10.1016/j.enzmictec.2023.110322"
}

Pantelić, L., Bogojević Škaro, S., Vojnović, S., Oliveira, R., Lazić, J., Ilić-Tomić, T., Milivojević, D.,& Nikodinović-Runić, J.. (2023). Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine. in Enzyme and Microbial Technology, 171, 110322.
https://doi.org/10.1016/j.enzmictec.2023.110322

Pantelić L, Bogojević Škaro S, Vojnović S, Oliveira R, Lazić J, Ilić-Tomić T, Milivojević D, Nikodinović-Runić J. Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine. in Enzyme and Microbial Technology. 2023;171:110322.
doi:10.1016/j.enzmictec.2023.110322 .

Pantelić, Lena, Bogojević Škaro, Sanja, Vojnović, Sandra, Oliveira, Rui, Lazić, Jelena, Ilić-Tomić, Tatjana, Milivojević, Dušan, Nikodinović-Runić, Jasmina, "Upcycling of food waste streams to valuable biopigments pyocyanin and 1-hydroxyphenazine" in Enzyme and Microbial Technology, 171 (2023):110322,
https://doi.org/10.1016/j.enzmictec.2023.110322 . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Filipović, Vuk
AU  - Topakas, Evangelos
AU  - Karnaouri, Anthi
AU  - Živković, Jelena
AU  - Krgović, Nemanja
AU  - Mudrić, Jelena
AU  - Savikin, Katarina
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2304-8158/12/16/2995
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2025
AB  - Background: Bacterial nanocellulose (BNC) has gained in popularity over the years due to its outstanding properties such as renewability, biocompatibility, and bioavailability, and its use as an eco-friendly material of the future for replacing petrochemical products. (2) Methods: This research refers to the utilization of lignocellulose coming from wood waste via enzymatic hydrolysis to produce biopolymer BNC with an accumulation rate of 0.09 mg/mL/day. Besides its significant contribution to the sustainability, circularity, and valorization of biomass products, the obtained BNC was functionalized through the adsorption of black raspberry extract (BR) by simple soaking. (3) Results: BR contained 77.25 ± 0.23 mg GAE/g of total phenolics and 27.42 ± 0.32 mg CGE/g of total anthocyanins. The antioxidant and antimicrobial activity of BR was evaluated by DPPH (60.51 ± 0.18 µg/mL) and FRAP (1.66 ± 0.03 mmol Fe2+/g) and using a standard disc diffusion assay, respectively. The successful synthesis and interactions between BNC and BR were confirmed by FTIR analysis, while the morphology of the new nutrient-enriched material was investigated by SEM analysis. Moreover, the in vitro release kinetics of a main active compound (cyanidin-3-O-rutinoside) was tested in different release media. (4) Conclusions: The upcycling process of lignocellulose into enriched BNC has been demonstrated. All findings emphasize the potential of BNC–BR as a sustainable food industry material.
T2  - Foods
T2  - Foods
T1  - Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient
IS  - 16
SP  - 2995
VL  - 12
DO  - 10.3390/foods12162995
ER  - 

@article{
author = "Ponjavić, Marijana and Filipović, Vuk and Topakas, Evangelos and Karnaouri, Anthi and Živković, Jelena and Krgović, Nemanja and Mudrić, Jelena and Savikin, Katarina and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Background: Bacterial nanocellulose (BNC) has gained in popularity over the years due to its outstanding properties such as renewability, biocompatibility, and bioavailability, and its use as an eco-friendly material of the future for replacing petrochemical products. (2) Methods: This research refers to the utilization of lignocellulose coming from wood waste via enzymatic hydrolysis to produce biopolymer BNC with an accumulation rate of 0.09 mg/mL/day. Besides its significant contribution to the sustainability, circularity, and valorization of biomass products, the obtained BNC was functionalized through the adsorption of black raspberry extract (BR) by simple soaking. (3) Results: BR contained 77.25 ± 0.23 mg GAE/g of total phenolics and 27.42 ± 0.32 mg CGE/g of total anthocyanins. The antioxidant and antimicrobial activity of BR was evaluated by DPPH (60.51 ± 0.18 µg/mL) and FRAP (1.66 ± 0.03 mmol Fe2+/g) and using a standard disc diffusion assay, respectively. The successful synthesis and interactions between BNC and BR were confirmed by FTIR analysis, while the morphology of the new nutrient-enriched material was investigated by SEM analysis. Moreover, the in vitro release kinetics of a main active compound (cyanidin-3-O-rutinoside) was tested in different release media. (4) Conclusions: The upcycling process of lignocellulose into enriched BNC has been demonstrated. All findings emphasize the potential of BNC–BR as a sustainable food industry material.",
journal = "Foods, Foods",
title = "Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient",
number = "16",
pages = "2995",
volume = "12",
doi = "10.3390/foods12162995"
}

Ponjavić, M., Filipović, V., Topakas, E., Karnaouri, A., Živković, J., Krgović, N., Mudrić, J., Savikin, K.,& Nikodinović-Runić, J.. (2023). Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient. in Foods, 12(16), 2995.
https://doi.org/10.3390/foods12162995

Ponjavić M, Filipović V, Topakas E, Karnaouri A, Živković J, Krgović N, Mudrić J, Savikin K, Nikodinović-Runić J. Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient. in Foods. 2023;12(16):2995.
doi:10.3390/foods12162995 .

Ponjavić, Marijana, Filipović, Vuk, Topakas, Evangelos, Karnaouri, Anthi, Živković, Jelena, Krgović, Nemanja, Mudrić, Jelena, Savikin, Katarina, Nikodinović-Runić, Jasmina, "Two-Step Upcycling Process of Lignocellulose into Edible Bacterial Nanocellulose with Black Raspberry Extract as an Active Ingredient" in Foods, 12, no. 16 (2023):2995,
https://doi.org/10.3390/foods12162995 . .

TY  - THES
AU  - Lazić, Jelena
PY  - 2022
UR  - https://repozitorij.ung.si/IzpisGradiva.php?id=7664&lang=eng
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1793
AB  - Prodigiosins (PGs) are a class of bacterial secondary metabolites with remarkable biological activities and colour. In this study, optimised fermentative production of prodigiosin (PG) using waste processed meat as a substrate has been achieved to levels of 83.1 ± 3.0 mg/L from a commercially available Serratia marcescens ATCC 27117 strain within 12 h. Methods were established for the reliable PG extraction from both the bacterial cell pellet and the culture supernatant, while gravitation column chromatography was used to obtain pure bacterial PG. The structure of the isolated PG was optimised by environmentally acceptable oxidative bromination reactions, obtaining mono- and dibrominated derivatives (PG-Br and PG-Br2). Chemical structures were confirmed by structural characterisation using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS), showing that PG-Br is a mixture of two monobrominated isomers in approximately equal ratios, while PG-Br2 was afforded as a pure derivative. PG and its brominated derivatives (Br-derivatives) showed anticancer potential with half-maximal inhibitory concentration (IC50) values ranging from 0.62 to 17.00 μg/mL on four tested cancer cell lines (A549 lung, A375 skin, MDA-MB-231 breast, HCT116 colon) and an induction of early apoptosis, but low selectivity against healthy cell lines (MRC-5 lung fibroblasts and HaCaT skin keratinocytes). All three PG, PG-Br and PG-Br2 compounds did not affect roundworms (Caenorhabditis elegans) at concentrations up to 50 μg/mL. However, an improved toxicity profile of Br-derivatives in comparison to the parent PG was observed in vivo using zebrafish (Danio rerio) model system, when 10 μg/mL applied at 6 h post fertilisation caused death rate of 100, 30 and 0% by PG, PG-Br and PG-Br2, respectively, which is a significant finding for further structural optimisations of bacterial PGs.
AB  - Prodigiozini (PG) so razred obarvanih sekundarnih bakterijskih metabolitov z izjemno biološko aktivnostjo. V doktorski raziskovalni nalogi smo za proizvodnjo prodigiozina uporabili komercialni sev bakterije Serratia marcescens ATCC 27117, kot substrat za fermentacijo pa odpadne produkte procesiranja mesa. Na ta način smo pridobili do 83.1 ± 3.0 prodigiozina v 12-ih urah fermentacije. V nadaljevanju smo vzpostavili metode za ekstrakcijo PS tako iz peleta bakterijskih celic kot tudi iz supernatanta bakterijske kulture. Za pridobitev čistega bakterijskega PG smo uporabili gravitacijsko kolonsko kromatografijo.
Strukturo izoliranega PG smo optimizirali z okolju prijazno metodo oksidativnega bromiranja, pri čemer smo dobili mono- in di-bromirane derivate (PG-Br in PG-Br2). Strukturna karakterizacija bromiranih spojin s pomočjo jedrske magnetne resonance (NMR) in masne spektrometrije (MS) je pokazala, da je PG-Br zmes dveh mono-bromiranih izomer v približno enakih razmerjih, medtem ko je bil PG-Br2 pridobljen kot čisti derivat.
PG in njegove bromirane derivate smo testirali na  štirih rakavih celičnih linijah (A549 – pljučne celice, A375 – kožne celice, MDA-MB-231 – celice raka na prsih in HCT116 – črevesne celice). Potrdili smo antitumorske lastnosti PG in njegovih bromiranih derivatov s polovičnimi maksimalnimi inhibitornimi koncentracijami (IC50) v razponu od 0,62 do 17,00 µg/mL ter indukcijo zgodnje apoptoze, vendar hkrati tudi nizko selektivnost proti zdravim celičnim linijam (MRC-5 - pljučni fibroblasti in HaCaT - kožni keratinociti). Nobena od testiranih PG, PG-Br in PG-Br2 spojin ni imela opaznega vpliva na gliste Caenorhabditis elegans pri koncentracijah do 50 µg/mL. Ugotovili pa smo manjšo toksičnost bromiranih derivatov PG v primerjavi z matičnim PG v modelnem sistemu rib cebric (Danio rerio) in vivo. Pri cebricah, ki so bile 6 ur po oploditvi izpostavljene 10 µg/mL PG, PG-Br oziroma PG-Br2, smo ugotovili 100%, 30% in 0% smrtnost, kar je pomembna ugotovitev za nadaljnjo strukturno optimizacijo bakterijskih PG.
PB  - University of Nova Gorica
T1  - Structure optimisation of biopigment prodigiosin from Serratia marcescens ATCC 27117 and antimicrobial and anticancer properties of novel halogenated derivatives
T1  - Strukturna optimizacija biopigmenta prodigiozin iz bakterije Serratia marcescens ATCC 27117 ter antimikrobne in antitumorske lastnosti novih halogeniranih derivatov
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1793
ER  - 

@phdthesis{
author = "Lazić, Jelena",
year = "2022",
abstract = "Prodigiosins (PGs) are a class of bacterial secondary metabolites with remarkable biological activities and colour. In this study, optimised fermentative production of prodigiosin (PG) using waste processed meat as a substrate has been achieved to levels of 83.1 ± 3.0 mg/L from a commercially available Serratia marcescens ATCC 27117 strain within 12 h. Methods were established for the reliable PG extraction from both the bacterial cell pellet and the culture supernatant, while gravitation column chromatography was used to obtain pure bacterial PG. The structure of the isolated PG was optimised by environmentally acceptable oxidative bromination reactions, obtaining mono- and dibrominated derivatives (PG-Br and PG-Br2). Chemical structures were confirmed by structural characterisation using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS), showing that PG-Br is a mixture of two monobrominated isomers in approximately equal ratios, while PG-Br2 was afforded as a pure derivative. PG and its brominated derivatives (Br-derivatives) showed anticancer potential with half-maximal inhibitory concentration (IC50) values ranging from 0.62 to 17.00 μg/mL on four tested cancer cell lines (A549 lung, A375 skin, MDA-MB-231 breast, HCT116 colon) and an induction of early apoptosis, but low selectivity against healthy cell lines (MRC-5 lung fibroblasts and HaCaT skin keratinocytes). All three PG, PG-Br and PG-Br2 compounds did not affect roundworms (Caenorhabditis elegans) at concentrations up to 50 μg/mL. However, an improved toxicity profile of Br-derivatives in comparison to the parent PG was observed in vivo using zebrafish (Danio rerio) model system, when 10 μg/mL applied at 6 h post fertilisation caused death rate of 100, 30 and 0% by PG, PG-Br and PG-Br2, respectively, which is a significant finding for further structural optimisations of bacterial PGs., Prodigiozini (PG) so razred obarvanih sekundarnih bakterijskih metabolitov z izjemno biološko aktivnostjo. V doktorski raziskovalni nalogi smo za proizvodnjo prodigiozina uporabili komercialni sev bakterije Serratia marcescens ATCC 27117, kot substrat za fermentacijo pa odpadne produkte procesiranja mesa. Na ta način smo pridobili do 83.1 ± 3.0 prodigiozina v 12-ih urah fermentacije. V nadaljevanju smo vzpostavili metode za ekstrakcijo PS tako iz peleta bakterijskih celic kot tudi iz supernatanta bakterijske kulture. Za pridobitev čistega bakterijskega PG smo uporabili gravitacijsko kolonsko kromatografijo.
Strukturo izoliranega PG smo optimizirali z okolju prijazno metodo oksidativnega bromiranja, pri čemer smo dobili mono- in di-bromirane derivate (PG-Br in PG-Br2). Strukturna karakterizacija bromiranih spojin s pomočjo jedrske magnetne resonance (NMR) in masne spektrometrije (MS) je pokazala, da je PG-Br zmes dveh mono-bromiranih izomer v približno enakih razmerjih, medtem ko je bil PG-Br2 pridobljen kot čisti derivat.
PG in njegove bromirane derivate smo testirali na  štirih rakavih celičnih linijah (A549 – pljučne celice, A375 – kožne celice, MDA-MB-231 – celice raka na prsih in HCT116 – črevesne celice). Potrdili smo antitumorske lastnosti PG in njegovih bromiranih derivatov s polovičnimi maksimalnimi inhibitornimi koncentracijami (IC50) v razponu od 0,62 do 17,00 µg/mL ter indukcijo zgodnje apoptoze, vendar hkrati tudi nizko selektivnost proti zdravim celičnim linijam (MRC-5 - pljučni fibroblasti in HaCaT - kožni keratinociti). Nobena od testiranih PG, PG-Br in PG-Br2 spojin ni imela opaznega vpliva na gliste Caenorhabditis elegans pri koncentracijah do 50 µg/mL. Ugotovili pa smo manjšo toksičnost bromiranih derivatov PG v primerjavi z matičnim PG v modelnem sistemu rib cebric (Danio rerio) in vivo. Pri cebricah, ki so bile 6 ur po oploditvi izpostavljene 10 µg/mL PG, PG-Br oziroma PG-Br2, smo ugotovili 100%, 30% in 0% smrtnost, kar je pomembna ugotovitev za nadaljnjo strukturno optimizacijo bakterijskih PG.",
publisher = "University of Nova Gorica",
title = "Structure optimisation of biopigment prodigiosin from Serratia marcescens ATCC 27117 and antimicrobial and anticancer properties of novel halogenated derivatives, Strukturna optimizacija biopigmenta prodigiozin iz bakterije Serratia marcescens ATCC 27117 ter antimikrobne in antitumorske lastnosti novih halogeniranih derivatov",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1793"
}

Lazić, J.. (2022). Structure optimisation of biopigment prodigiosin from Serratia marcescens ATCC 27117 and antimicrobial and anticancer properties of novel halogenated derivatives. 
University of Nova Gorica..
https://hdl.handle.net/21.15107/rcub_imagine_1793

Lazić J. Structure optimisation of biopigment prodigiosin from Serratia marcescens ATCC 27117 and antimicrobial and anticancer properties of novel halogenated derivatives. 2022;.
https://hdl.handle.net/21.15107/rcub_imagine_1793 .

Lazić, Jelena, "Structure optimisation of biopigment prodigiosin from Serratia marcescens ATCC 27117 and antimicrobial and anticancer properties of novel halogenated derivatives" (2022),
https://hdl.handle.net/21.15107/rcub_imagine_1793 .

TY  - GEN
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2270
AB  - Available biologics have revolutionized cancer treatment, proven efficient in complex
infections, changed the lives of people with rare diseases, and offered hope for many patients
who previously had no effective treatment options for their condition. Downside is that these
biopharmaceuticals are extremely expensive and as such, not widely available. BioECOLogics
will provide economic and environmental value via novel bio-upcycling and innovative
formulations to obtain next generation eco-friendly therapeutics. The project will exploit the
great potential of bacterially derived natural products (pyocyanin, pyrrolnitrin, pyoverdines,
undecylprodigiosin and staurosporine) with proven bioactivities (i.e. anticancer, antifungal,
antibiofilm, antiviral) to generate value-added biologics. Functional 100% bio-based biologicals
exhibiting advanced properties (improved activity, sustained release, synergy with essential and
noble metal ions, reduced price and carbon footprint) will be produced, formulated and
processed using innovative techniques such as fermentative bioprocess intensification,
structural optimization via biocatalysis and formulations using essential and noble metals, as
well as biopolymeric drug carriers. Therefore, BioECOLogics will result in a set of unique
products that offer real solutions for a spectrum of biomedical problems. In doing so, the
project contributes towards the shortage of novel biomedicines, but at the same time,
recognizes EU’s 2050 long-term strategy for a climate-neutral Europe by replacing fossil-based
material with a bio-based, renewable material. Novel bio-upcycling routes will be developed
based on the biotechnological valorization of waste materials into new formulations of
biopharmaceuticals resulting from bacterial fermentations and innovative formulations of
obtained molecules. The project approach will be transdisciplinary by combining microbiology,
biotechnology, process and inorganic chemistry, and pharmaceuticals knowledge. In addition to
the multidisciplinary nature, the knowledge transfers between researchers, industrial and other
stakeholders will contribute positively to the overall relevance of the impact for dissemination
and exploitation of results.
T2  - Science Fund of the Republic of Serbia, Program IDEAS
T1  - Value-added biologics through eco-sustainable routes (BioECOLogics)
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2270
ER  - 

@misc{
year = "2021",
abstract = "Available biologics have revolutionized cancer treatment, proven efficient in complex
infections, changed the lives of people with rare diseases, and offered hope for many patients
who previously had no effective treatment options for their condition. Downside is that these
biopharmaceuticals are extremely expensive and as such, not widely available. BioECOLogics
will provide economic and environmental value via novel bio-upcycling and innovative
formulations to obtain next generation eco-friendly therapeutics. The project will exploit the
great potential of bacterially derived natural products (pyocyanin, pyrrolnitrin, pyoverdines,
undecylprodigiosin and staurosporine) with proven bioactivities (i.e. anticancer, antifungal,
antibiofilm, antiviral) to generate value-added biologics. Functional 100% bio-based biologicals
exhibiting advanced properties (improved activity, sustained release, synergy with essential and
noble metal ions, reduced price and carbon footprint) will be produced, formulated and
processed using innovative techniques such as fermentative bioprocess intensification,
structural optimization via biocatalysis and formulations using essential and noble metals, as
well as biopolymeric drug carriers. Therefore, BioECOLogics will result in a set of unique
products that offer real solutions for a spectrum of biomedical problems. In doing so, the
project contributes towards the shortage of novel biomedicines, but at the same time,
recognizes EU’s 2050 long-term strategy for a climate-neutral Europe by replacing fossil-based
material with a bio-based, renewable material. Novel bio-upcycling routes will be developed
based on the biotechnological valorization of waste materials into new formulations of
biopharmaceuticals resulting from bacterial fermentations and innovative formulations of
obtained molecules. The project approach will be transdisciplinary by combining microbiology,
biotechnology, process and inorganic chemistry, and pharmaceuticals knowledge. In addition to
the multidisciplinary nature, the knowledge transfers between researchers, industrial and other
stakeholders will contribute positively to the overall relevance of the impact for dissemination
and exploitation of results.",
journal = "Science Fund of the Republic of Serbia, Program IDEAS",
title = "Value-added biologics through eco-sustainable routes (BioECOLogics)",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2270"
}

(2021). Value-added biologics through eco-sustainable routes (BioECOLogics). in Science Fund of the Republic of Serbia, Program IDEAS.
https://hdl.handle.net/21.15107/rcub_imagine_2270

Value-added biologics through eco-sustainable routes (BioECOLogics). in Science Fund of the Republic of Serbia, Program IDEAS. 2021;.
https://hdl.handle.net/21.15107/rcub_imagine_2270 .

"Value-added biologics through eco-sustainable routes (BioECOLogics)" in Science Fund of the Republic of Serbia, Program IDEAS (2021),
https://hdl.handle.net/21.15107/rcub_imagine_2270 .