TY  - CONF
AU  - Ćirić, Milica
AU  - Budin, Clémence
AU  - de Boer, Tjalf
AU  - Pantelić, Brana
PY  - 2024
UR  - www.belbi.bg.ac.rs
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2464
AB  - alleviate plastic pollution are underway. Despite a proposed overlap between enzymatic
capacity to degrade plastics and lignocellulosic biomass, bioprospecting efforts to identify
microorganisms capable of degrading both types of substrates remain limited.
Plastic and lignocellulose degrading potential of 15 bacterial isolates from polluted Serbian
soils, belonging to genera detected with abundance >1% in virgin plastics (LLDPE)- or postconsumer
representative mixed plastics (MS)-enriched 16S metagenomes, was explored.
Plastic polymers Impranil® DLN-SD (SD) and DL2077 (DL), bis(2-hydroxyethyl) terephthalate
(BHET), polycaprolactone diol (PCL) and polylactic acid (PLA) and lignocellulosic polymers
carboxymethyl cellulose (CMC), arabinoxylan (AXYL) and lignin (LIG) were provided as sole
carbon source for the isolates.
16S rDNA dendrogram of 9 LLDPE-enriched isolates from genera Lysinibacillus, Rhodococcus,
Hydrogenophaga, Pseudomonas, Nocardoides and Psychrobacillus and 6 MS-enriched isolates
from genera Pseudomonas, Advenella and Paenibacillus showed no clear grouping, suggesting
that relatively distinct isolates were selected for the screening. No zones of clearing, indicating
complete substrate degradation is taking place, were observed. Single Advenella isolate
demonstrated growth on all plastic substrates, while 11 isolates demonstrated growth on PCL,
10 on BHET, 6 on SD, 4 on DL and 4 on PLA. Fourteen isolates grew on all tested lignocellulosic
substrates.
Genome mining of 3 sequenced isolates using PAZy database identified putative PU-, PET, PCLand
PLA-active enzymes in both Hydrogenophaga, growing on all plastic substrates except PLA
and Pseudomonas, growing on BHET and PCL, while Lysinibacillus, with predicted PCL and PLA
activities, demonstrated no growth on any of the tested plastic substrates. Lignocelullolytic
enzymes (GHs, CEs, PLs and AAs) were also predicted in these isolates, demonstrating growth
on all tested lignocellulosic substrates, using CAZy database.
Investigated isolates should be further explored using a wider range of plastic substrates and
screening conditions.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 5th Belgrade Bioinformatics Conference
T1  - Exploring biotechnological potential of LLDPE- and mixed plastics-degrading bacteria from contaminated soils
EP  - 89
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2464
ER  - 

@conference{
author = "Ćirić, Milica and Budin, Clémence and de Boer, Tjalf and Pantelić, Brana",
year = "2024",
abstract = "alleviate plastic pollution are underway. Despite a proposed overlap between enzymatic
capacity to degrade plastics and lignocellulosic biomass, bioprospecting efforts to identify
microorganisms capable of degrading both types of substrates remain limited.
Plastic and lignocellulose degrading potential of 15 bacterial isolates from polluted Serbian
soils, belonging to genera detected with abundance >1% in virgin plastics (LLDPE)- or postconsumer
representative mixed plastics (MS)-enriched 16S metagenomes, was explored.
Plastic polymers Impranil® DLN-SD (SD) and DL2077 (DL), bis(2-hydroxyethyl) terephthalate
(BHET), polycaprolactone diol (PCL) and polylactic acid (PLA) and lignocellulosic polymers
carboxymethyl cellulose (CMC), arabinoxylan (AXYL) and lignin (LIG) were provided as sole
carbon source for the isolates.
16S rDNA dendrogram of 9 LLDPE-enriched isolates from genera Lysinibacillus, Rhodococcus,
Hydrogenophaga, Pseudomonas, Nocardoides and Psychrobacillus and 6 MS-enriched isolates
from genera Pseudomonas, Advenella and Paenibacillus showed no clear grouping, suggesting
that relatively distinct isolates were selected for the screening. No zones of clearing, indicating
complete substrate degradation is taking place, were observed. Single Advenella isolate
demonstrated growth on all plastic substrates, while 11 isolates demonstrated growth on PCL,
10 on BHET, 6 on SD, 4 on DL and 4 on PLA. Fourteen isolates grew on all tested lignocellulosic
substrates.
Genome mining of 3 sequenced isolates using PAZy database identified putative PU-, PET, PCLand
PLA-active enzymes in both Hydrogenophaga, growing on all plastic substrates except PLA
and Pseudomonas, growing on BHET and PCL, while Lysinibacillus, with predicted PCL and PLA
activities, demonstrated no growth on any of the tested plastic substrates. Lignocelullolytic
enzymes (GHs, CEs, PLs and AAs) were also predicted in these isolates, demonstrating growth
on all tested lignocellulosic substrates, using CAZy database.
Investigated isolates should be further explored using a wider range of plastic substrates and
screening conditions.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "5th Belgrade Bioinformatics Conference",
title = "Exploring biotechnological potential of LLDPE- and mixed plastics-degrading bacteria from contaminated soils",
pages = "89-89",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2464"
}

Ćirić, M., Budin, C., de Boer, T.,& Pantelić, B.. (2024). Exploring biotechnological potential of LLDPE- and mixed plastics-degrading bacteria from contaminated soils. in 5th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 89-89.
https://hdl.handle.net/21.15107/rcub_imagine_2464

Ćirić M, Budin C, de Boer T, Pantelić B. Exploring biotechnological potential of LLDPE- and mixed plastics-degrading bacteria from contaminated soils. in 5th Belgrade Bioinformatics Conference. 2024;:89-89.
https://hdl.handle.net/21.15107/rcub_imagine_2464 .

Ćirić, Milica, Budin, Clémence, de Boer, Tjalf, Pantelić, Brana, "Exploring biotechnological potential of LLDPE- and mixed plastics-degrading bacteria from contaminated soils" in 5th Belgrade Bioinformatics Conference (2024):89-89,
https://hdl.handle.net/21.15107/rcub_imagine_2464 .