TY  - CONF
AU  - Marija, Nenadović
AU  - Pantelić, Brana
AU  - Ponjavić, Marijana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1916
AB  - Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics,
owing to their biological origin, biodegradability, biocompatibility and structural
diversity. However, environmental biodegradation of PHA is achieved in a time
frame of several months to several years, depending on environmental conditions,
and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into
account the high production cost of PHA, landfilling at the end of life is not likely to
be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly
bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ
properties to create suitable biocatalysts for the industrially relevant time frame and
operating conditions. In order to do so, we decided to randomize PhaZ sequences
and functionally screen enzyme variants for accelerated PHA degradation and
improved biocatalyst stability. Up to this day, various phaZ genes have been mutated
solely for mechanistic purposes eg. Catalytic residue identification, and elucidation
of the substrate recognition process [2,3,4,5,6,7].
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers
EP  - 196
SP  - 195
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1916
ER  - 

@conference{
author = "Marija, Nenadović and Pantelić, Brana and Ponjavić, Marijana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics,
owing to their biological origin, biodegradability, biocompatibility and structural
diversity. However, environmental biodegradation of PHA is achieved in a time
frame of several months to several years, depending on environmental conditions,
and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into
account the high production cost of PHA, landfilling at the end of life is not likely to
be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly
bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ
properties to create suitable biocatalysts for the industrially relevant time frame and
operating conditions. In order to do so, we decided to randomize PhaZ sequences
and functionally screen enzyme variants for accelerated PHA degradation and
improved biocatalyst stability. Up to this day, various phaZ genes have been mutated
solely for mechanistic purposes eg. Catalytic residue identification, and elucidation
of the substrate recognition process [2,3,4,5,6,7].",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers",
pages = "196-195",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1916"
}

Marija, N., Pantelić, B., Ponjavić, M.,& Nikodinović-Runić, J.. (2023). Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 195-196.
https://hdl.handle.net/21.15107/rcub_imagine_1916

Marija N, Pantelić B, Ponjavić M, Nikodinović-Runić J. Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:195-196.
https://hdl.handle.net/21.15107/rcub_imagine_1916 .

Marija, Nenadović, Pantelić, Brana, Ponjavić, Marijana, Nikodinović-Runić, Jasmina, "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):195-196,
https://hdl.handle.net/21.15107/rcub_imagine_1916 .