TY  - JOUR
AU  - Delasoie, Joachim
AU  - Radaković, Nataša
AU  - Pavić, Aleksandar
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1335
AB  - Silica microparticles made of diatomaceous earth have become particularly attractive materials for designing drug delivery systems. In order to investigate the use of natural diatoms as drug scaffolds for carbon monoxide releasing molecules (CORMs), we evaluated the chemisorption of the cis-[Re(CO)(2)Br-4](2-) complex (ReCORM-2) and its vitamin B-12 derivative (B-12-ReCORM-2) on Coscinodiscus frustules by 3D FT-IR spectroscopic imaging, and the drugs' neovascularization effects in vivo in the zebrafish (Danio rerio) model. By mapping the symmetric Re-C equivalent to O upsilon(CO) stretching vibration of the CORMs in the 2000 cm(-1) region, we found that the drugs are mostly localized at the girdle band of the diatom frustule. Both ReCORM-2 and B-12-ReCORM-2 retain their CO-releasing ability when chemisorbed on the diatoms. When applied in vivo at doses  gt = 25 mu M, the molecules markedly reduced intersegmental and subintestinal vessels development in zebrafish, revealing high anti-angiogenic potential. In addition, diatom frustules did not provoke any toxic in vivo response in the zebrafish embryos, including inflammation. Overall, our results indicate that: (1) CORMs chemisorbed on diatom frustules retain their CO-releasing abilities; (2) both CO-releasing molecules show a concentration-dependent effect on the neovascularization in developing zebrafish; (3) silicate frustules are not toxic and could be used as CORMs drug carriers.
PB  - MDPI, Basel
T2  - Applied Sciences-Basel
T1  - Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging
IS  - 20
VL  - 10
DO  - 10.3390/app10207380
ER  - 

@article{
author = "Delasoie, Joachim and Radaković, Nataša and Pavić, Aleksandar and Zobi, Fabio",
year = "2020",
abstract = "Silica microparticles made of diatomaceous earth have become particularly attractive materials for designing drug delivery systems. In order to investigate the use of natural diatoms as drug scaffolds for carbon monoxide releasing molecules (CORMs), we evaluated the chemisorption of the cis-[Re(CO)(2)Br-4](2-) complex (ReCORM-2) and its vitamin B-12 derivative (B-12-ReCORM-2) on Coscinodiscus frustules by 3D FT-IR spectroscopic imaging, and the drugs' neovascularization effects in vivo in the zebrafish (Danio rerio) model. By mapping the symmetric Re-C equivalent to O upsilon(CO) stretching vibration of the CORMs in the 2000 cm(-1) region, we found that the drugs are mostly localized at the girdle band of the diatom frustule. Both ReCORM-2 and B-12-ReCORM-2 retain their CO-releasing ability when chemisorbed on the diatoms. When applied in vivo at doses  gt = 25 mu M, the molecules markedly reduced intersegmental and subintestinal vessels development in zebrafish, revealing high anti-angiogenic potential. In addition, diatom frustules did not provoke any toxic in vivo response in the zebrafish embryos, including inflammation. Overall, our results indicate that: (1) CORMs chemisorbed on diatom frustules retain their CO-releasing abilities; (2) both CO-releasing molecules show a concentration-dependent effect on the neovascularization in developing zebrafish; (3) silicate frustules are not toxic and could be used as CORMs drug carriers.",
publisher = "MDPI, Basel",
journal = "Applied Sciences-Basel",
title = "Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging",
number = "20",
volume = "10",
doi = "10.3390/app10207380"
}

Delasoie, J., Radaković, N., Pavić, A.,& Zobi, F.. (2020). Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging. in Applied Sciences-Basel
MDPI, Basel., 10(20).
https://doi.org/10.3390/app10207380

Delasoie J, Radaković N, Pavić A, Zobi F. Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging. in Applied Sciences-Basel. 2020;10(20).
doi:10.3390/app10207380 .

Delasoie, Joachim, Radaković, Nataša, Pavić, Aleksandar, Zobi, Fabio, "Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging" in Applied Sciences-Basel, 10, no. 20 (2020),
https://doi.org/10.3390/app10207380 . .

TY  - JOUR
AU  - Delasoie, Joachim
AU  - Pavić, Aleksandar
AU  - Voutier, Noemie
AU  - Vojnović, Sandra
AU  - Crochet, Aurelien
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1612
AB  - Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma
VL  - 204
DO  - 10.1016/j.ejmech.2020.112583
ER  - 

@article{
author = "Delasoie, Joachim and Pavić, Aleksandar and Voutier, Noemie and Vojnović, Sandra and Crochet, Aurelien and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2020",
abstract = "Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma",
volume = "204",
doi = "10.1016/j.ejmech.2020.112583"
}

Delasoie, J., Pavić, A., Voutier, N., Vojnović, S., Crochet, A., Nikodinović-Runić, J.,& Zobi, F.. (2020). Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 204.
https://doi.org/10.1016/j.ejmech.2020.112583

Delasoie J, Pavić A, Voutier N, Vojnović S, Crochet A, Nikodinović-Runić J, Zobi F. Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry. 2020;204.
doi:10.1016/j.ejmech.2020.112583 .

Delasoie, Joachim, Pavić, Aleksandar, Voutier, Noemie, Vojnović, Sandra, Crochet, Aurelien, Nikodinović-Runić, Jasmina, Zobi, Fabio, "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma" in European Journal of Medicinal Chemistry, 204 (2020),
https://doi.org/10.1016/j.ejmech.2020.112583 . .

TY  - JOUR
AU  - Delasoie, Joachim
AU  - Pavić, Aleksandar
AU  - Voutier, Noemie
AU  - Vojnović, Sandra
AU  - Crochet, Aurelien
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1360
AB  - Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma
VL  - 204
DO  - 10.1016/j.ejmech.2020.112583
ER  - 

@article{
author = "Delasoie, Joachim and Pavić, Aleksandar and Voutier, Noemie and Vojnović, Sandra and Crochet, Aurelien and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2020",
abstract = "Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma",
volume = "204",
doi = "10.1016/j.ejmech.2020.112583"
}

Delasoie, J., Pavić, A., Voutier, N., Vojnović, S., Crochet, A., Nikodinović-Runić, J.,& Zobi, F.. (2020). Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 204.
https://doi.org/10.1016/j.ejmech.2020.112583

Delasoie J, Pavić A, Voutier N, Vojnović S, Crochet A, Nikodinović-Runić J, Zobi F. Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry. 2020;204.
doi:10.1016/j.ejmech.2020.112583 .

Delasoie, Joachim, Pavić, Aleksandar, Voutier, Noemie, Vojnović, Sandra, Crochet, Aurelien, Nikodinović-Runić, Jasmina, Zobi, Fabio, "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma" in European Journal of Medicinal Chemistry, 204 (2020),
https://doi.org/10.1016/j.ejmech.2020.112583 . .

TY  - JOUR
AU  - Delasoie, Joachim
AU  - Schiel, Philippe
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1301
AB  - Systemic toxicity and severe side effects are commonly associated with anticancer chemotherapies. New strategies based on enhanced drug selectivity and targeted delivery to cancer cells while leaving healthy tissue undamaged can reduce the global patient burden. Herein, we report the design, synthesis and characterization of a bio-inspired hybrid multifunctional drug delivery system based on diatom microalgae. The microalgae's surface was chemically functionalized with hybrid vitamin B-12-photoactivatable molecules and the materials further loaded with highly active rhenium(I) tricarbonyl anticancer complexes. The constructs showed enhanced adherence to colorectal cancer (CRC) cells and slow release of the chemotherapeutic drugs. The overall toxicity of the hybrid multifunctional drug delivery system was further enhanced by photoactivation of the microalgae surface. Depending on the construct and anticancer drug, a 2-fold increase in the cytotoxic efficacy of the drug was observed upon light irradiation. The use of this targeted drug delivery strategy, together with selective spatial-temporal light activation, may lead to lower effective concentration of anticancer drugs, thereby reducing medication doses, possible side effects and overall burden for the patient.
PB  - MDPI, Basel
T2  - Pharmaceutics
T1  - Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes
IS  - 5
VL  - 12
DO  - 10.3390/pharmaceutics12050480
ER  - 

@article{
author = "Delasoie, Joachim and Schiel, Philippe and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2020",
abstract = "Systemic toxicity and severe side effects are commonly associated with anticancer chemotherapies. New strategies based on enhanced drug selectivity and targeted delivery to cancer cells while leaving healthy tissue undamaged can reduce the global patient burden. Herein, we report the design, synthesis and characterization of a bio-inspired hybrid multifunctional drug delivery system based on diatom microalgae. The microalgae's surface was chemically functionalized with hybrid vitamin B-12-photoactivatable molecules and the materials further loaded with highly active rhenium(I) tricarbonyl anticancer complexes. The constructs showed enhanced adherence to colorectal cancer (CRC) cells and slow release of the chemotherapeutic drugs. The overall toxicity of the hybrid multifunctional drug delivery system was further enhanced by photoactivation of the microalgae surface. Depending on the construct and anticancer drug, a 2-fold increase in the cytotoxic efficacy of the drug was observed upon light irradiation. The use of this targeted drug delivery strategy, together with selective spatial-temporal light activation, may lead to lower effective concentration of anticancer drugs, thereby reducing medication doses, possible side effects and overall burden for the patient.",
publisher = "MDPI, Basel",
journal = "Pharmaceutics",
title = "Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes",
number = "5",
volume = "12",
doi = "10.3390/pharmaceutics12050480"
}

Delasoie, J., Schiel, P., Vojnović, S., Nikodinović-Runić, J.,& Zobi, F.. (2020). Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes. in Pharmaceutics
MDPI, Basel., 12(5).
https://doi.org/10.3390/pharmaceutics12050480

Delasoie J, Schiel P, Vojnović S, Nikodinović-Runić J, Zobi F. Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes. in Pharmaceutics. 2020;12(5).
doi:10.3390/pharmaceutics12050480 .

Delasoie, Joachim, Schiel, Philippe, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Zobi, Fabio, "Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes" in Pharmaceutics, 12, no. 5 (2020),
https://doi.org/10.3390/pharmaceutics12050480 . .

TY  - JOUR
AU  - Rossier, Jeremie
AU  - Sovari, Sara Nasiri
AU  - Pavić, Aleksandar
AU  - Vojnović, Sandra
AU  - Stringer, Tameryn
AU  - Battig, Sarah
AU  - Smith, Gregory S.
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1276
AB  - We have explored the possibility of using organometallic derivatives of cobalamin as a scaffold for the delivery of the same antimalarial drug to both erythro- and hepatocytes. This hybrid molecule approach, intended as a possible tool for the development of multi-stage antimalarial agents, pivots on the preparation of azide-functionalized drugs which, after coupling to the vitamin, are released with a 4-(4-ethynylphenyl)-triazole functionality. Three chloroquine and one imidazolopiperazine derivative (based on the KAF156 structure) were selected as model drugs. One hybrid chloroquine conjugate was extensively studied via fluorescent labelling for in vitro and in vivo bio-distribution studies and gave proof-of-concept for the design. It showed no toxicity in vivo (zebrafish model) as well as no hepatotoxicity, no cardiotoxicity or developmental toxicity of the embryos. All 4-(4-ethynylphenyl)-triazole derivatives of chloroquine were equally active against chloroquine-resistant (CQR) and chloroquine-sensitive (CQS) Plasmodium falciparum strains.
PB  - MDPI, Basel
T2  - Molecules
T1  - Antiplasmodial Activity and In Vivo Bio-Distribution of Chloroquine Molecules Released with a 4-(4-Ethynylphenyl)-Triazole Moiety from Organometallo-Cobalamins
IS  - 12
VL  - 24
DO  - 10.3390/molecules24122310
ER  - 

@article{
author = "Rossier, Jeremie and Sovari, Sara Nasiri and Pavić, Aleksandar and Vojnović, Sandra and Stringer, Tameryn and Battig, Sarah and Smith, Gregory S. and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2019",
abstract = "We have explored the possibility of using organometallic derivatives of cobalamin as a scaffold for the delivery of the same antimalarial drug to both erythro- and hepatocytes. This hybrid molecule approach, intended as a possible tool for the development of multi-stage antimalarial agents, pivots on the preparation of azide-functionalized drugs which, after coupling to the vitamin, are released with a 4-(4-ethynylphenyl)-triazole functionality. Three chloroquine and one imidazolopiperazine derivative (based on the KAF156 structure) were selected as model drugs. One hybrid chloroquine conjugate was extensively studied via fluorescent labelling for in vitro and in vivo bio-distribution studies and gave proof-of-concept for the design. It showed no toxicity in vivo (zebrafish model) as well as no hepatotoxicity, no cardiotoxicity or developmental toxicity of the embryos. All 4-(4-ethynylphenyl)-triazole derivatives of chloroquine were equally active against chloroquine-resistant (CQR) and chloroquine-sensitive (CQS) Plasmodium falciparum strains.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Antiplasmodial Activity and In Vivo Bio-Distribution of Chloroquine Molecules Released with a 4-(4-Ethynylphenyl)-Triazole Moiety from Organometallo-Cobalamins",
number = "12",
volume = "24",
doi = "10.3390/molecules24122310"
}

Rossier, J., Sovari, S. N., Pavić, A., Vojnović, S., Stringer, T., Battig, S., Smith, G. S., Nikodinović-Runić, J.,& Zobi, F.. (2019). Antiplasmodial Activity and In Vivo Bio-Distribution of Chloroquine Molecules Released with a 4-(4-Ethynylphenyl)-Triazole Moiety from Organometallo-Cobalamins. in Molecules
MDPI, Basel., 24(12).
https://doi.org/10.3390/molecules24122310

Rossier J, Sovari SN, Pavić A, Vojnović S, Stringer T, Battig S, Smith GS, Nikodinović-Runić J, Zobi F. Antiplasmodial Activity and In Vivo Bio-Distribution of Chloroquine Molecules Released with a 4-(4-Ethynylphenyl)-Triazole Moiety from Organometallo-Cobalamins. in Molecules. 2019;24(12).
doi:10.3390/molecules24122310 .

Rossier, Jeremie, Sovari, Sara Nasiri, Pavić, Aleksandar, Vojnović, Sandra, Stringer, Tameryn, Battig, Sarah, Smith, Gregory S., Nikodinović-Runić, Jasmina, Zobi, Fabio, "Antiplasmodial Activity and In Vivo Bio-Distribution of Chloroquine Molecules Released with a 4-(4-Ethynylphenyl)-Triazole Moiety from Organometallo-Cobalamins" in Molecules, 24, no. 12 (2019),
https://doi.org/10.3390/molecules24122310 . .