TY  - JOUR
AU  - Tomić, Simonida
AU  - Vuković, Jovana
AU  - Babić Radić, Marija
AU  - Filipović, Vuk
AU  - Živanović, Dubravka
AU  - Nikolić, Miloš
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4360/15/3/589
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1788
AB  - Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.
PB  - MDPI
T2  - Polymers
T2  - Polymers
T1  - Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration
IS  - 3
SP  - 589
VL  - 15
DO  - 10.3390/polym15030589
ER  - 

@article{
author = "Tomić, Simonida and Vuković, Jovana and Babić Radić, Marija and Filipović, Vuk and Živanović, Dubravka and Nikolić, Miloš and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Scaffolding biomaterials are gaining great importance due to their beneficial properties for medical purposes. Targeted biomaterial engineering strategies through the synergy of different material types can be applied to design hybrid scaffolding biomaterials with advantageous properties for biomedical applications. In our research, a novel combination of the bioactive agent Manuka honey (MHo) with 2-hydroxyethyl methacrylate/gelatin (HG) hydrogel scaffolds was created as an efficient bioactive platform for biomedical applications. The effects of Manuka honey content on structural characteristics, porosity, swelling performance, in vitro degradation, and in vitro biocompatibility (fibroblast and keratinocyte cell lines) of hybrid hydrogel scaffolds were studied using Fourier transform infrared spectroscopy, the gravimetric method, and in vitro MTT biocompatibility assays. The engineered hybrid hydrogel scaffolds show advantageous properties, including porosity in the range of 71.25% to 90.09%, specific pH- and temperature-dependent swelling performance, and convenient absorption capacity. In vitro degradation studies showed scaffold degradability ranging from 6.27% to 27.18% for four weeks. In vitro biocompatibility assays on healthy human fibroblast (MRC5 cells) and keratinocyte (HaCaT cells) cell lines by MTT tests showed that cell viability depends on the Manuka honey content loaded in the HG hydrogel scaffolds. A sample containing the highest Manuka honey content (30%) exhibited the best biocompatible properties. The obtained results reveal that the synergy of the bioactive agent, Manuka honey, with 2-hydroxyethyl methacrylate/gelatin as hybrid hydrogel scaffolds has potential for biomedical purposes. By tuning the Manuka honey content in HG hydrogel scaffolds advantageous properties of hybrid scaffolds can be achieved for biomedical applications.",
publisher = "MDPI",
journal = "Polymers, Polymers",
title = "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration",
number = "3",
pages = "589",
volume = "15",
doi = "10.3390/polym15030589"
}

Tomić, S., Vuković, J., Babić Radić, M., Filipović, V., Živanović, D., Nikolić, M.,& Nikodinović-Runić, J.. (2023). Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers
MDPI., 15(3), 589.
https://doi.org/10.3390/polym15030589

Tomić S, Vuković J, Babić Radić M, Filipović V, Živanović D, Nikolić M, Nikodinović-Runić J. Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration. in Polymers. 2023;15(3):589.
doi:10.3390/polym15030589 .

Tomić, Simonida, Vuković, Jovana, Babić Radić, Marija, Filipović, Vuk, Živanović, Dubravka, Nikolić, Miloš, Nikodinović-Runić, Jasmina, "Manuka Honey/2-Hydroxyethyl Methacrylate/Gelatin Hybrid Hydrogel Scaffolds for Potential Tissue Regeneration" in Polymers, 15, no. 3 (2023):589,
https://doi.org/10.3390/polym15030589 . .

TY  - JOUR
AU  - Tomić, Simonida Lj
AU  - Nikodinović-Runić, Jasmina
AU  - Vukomanović, Marija
AU  - Babić, Marija M.
AU  - Vuković, Jovana S.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1450
AB  - Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material's composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.
PB  - MDPI, Basel
T2  - Polymers
T1  - Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite
IS  - 6
VL  - 13
DO  - 10.3390/polym13060932
ER  - 

@article{
author = "Tomić, Simonida Lj and Nikodinović-Runić, Jasmina and Vukomanović, Marija and Babić, Marija M. and Vuković, Jovana S.",
year = "2021",
abstract = "Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material's composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.",
publisher = "MDPI, Basel",
journal = "Polymers",
title = "Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite",
number = "6",
volume = "13",
doi = "10.3390/polym13060932"
}

Tomić, S. L., Nikodinović-Runić, J., Vukomanović, M., Babić, M. M.,& Vuković, J. S.. (2021). Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. in Polymers
MDPI, Basel., 13(6).
https://doi.org/10.3390/polym13060932

Tomić SL, Nikodinović-Runić J, Vukomanović M, Babić MM, Vuković JS. Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. in Polymers. 2021;13(6).
doi:10.3390/polym13060932 .

Tomić, Simonida Lj, Nikodinović-Runić, Jasmina, Vukomanović, Marija, Babić, Marija M., Vuković, Jovana S., "Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite" in Polymers, 13, no. 6 (2021),
https://doi.org/10.3390/polym13060932 . .

TY  - JOUR
AU  - Tomić, Simonida Lj.
AU  - Babić, Marija M.
AU  - Vuković, Jovana S.
AU  - Đokić, Lidija
AU  - Pavić, Aleksandar
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1361
AB  - We have studied the effect of compositions and methods of preparation on the physico-chemical and biocompatible behavior of the hydrogel matrices. Hydrogel matrices are synthesized by free radical polymerization of 2-hydroxyethyl methacrylate net and with gelatin. Highly porous hydrogel structures were obtained by porogenation, and by cryogenic treatment followed by freeze-drying. All samples were characterized for structural, morphological, absorption, degradation and in vitro (healthy human fibroblast cell line) and in vivo (zebrafishDanio rerio) biocompatible properties. The obtained results show that cryo samples, especially with gelatin show better, favorable absorption, morphological and biocompatible properties in comparison with PHEMA samples, which makes these materials highly attractive for biomedical uses.
PB  - Springer, Dordrecht
T2  - Journal of Polymer Research
T1  - Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties
IS  - 10
VL  - 27
DO  - 10.1007/s10965-020-02219-w
ER  - 

@article{
author = "Tomić, Simonida Lj. and Babić, Marija M. and Vuković, Jovana S. and Đokić, Lidija and Pavić, Aleksandar and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "We have studied the effect of compositions and methods of preparation on the physico-chemical and biocompatible behavior of the hydrogel matrices. Hydrogel matrices are synthesized by free radical polymerization of 2-hydroxyethyl methacrylate net and with gelatin. Highly porous hydrogel structures were obtained by porogenation, and by cryogenic treatment followed by freeze-drying. All samples were characterized for structural, morphological, absorption, degradation and in vitro (healthy human fibroblast cell line) and in vivo (zebrafishDanio rerio) biocompatible properties. The obtained results show that cryo samples, especially with gelatin show better, favorable absorption, morphological and biocompatible properties in comparison with PHEMA samples, which makes these materials highly attractive for biomedical uses.",
publisher = "Springer, Dordrecht",
journal = "Journal of Polymer Research",
title = "Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties",
number = "10",
volume = "27",
doi = "10.1007/s10965-020-02219-w"
}

Tomić, S. Lj., Babić, M. M., Vuković, J. S., Đokić, L., Pavić, A.,& Nikodinović-Runić, J.. (2020). Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties. in Journal of Polymer Research
Springer, Dordrecht., 27(10).
https://doi.org/10.1007/s10965-020-02219-w

Tomić SL, Babić MM, Vuković JS, Đokić L, Pavić A, Nikodinović-Runić J. Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties. in Journal of Polymer Research. 2020;27(10).
doi:10.1007/s10965-020-02219-w .

Tomić, Simonida Lj., Babić, Marija M., Vuković, Jovana S., Đokić, Lidija, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, "Effect of composition and method of preparation of 2-hydroxyethyl methacrylate/gelatin hydrogels on biological in vitro (cell line) and in vivo (zebrafish) properties" in Journal of Polymer Research, 27, no. 10 (2020),
https://doi.org/10.1007/s10965-020-02219-w . .

TY  - JOUR
AU  - Babić, Marija M.
AU  - Vukomanović, Marija
AU  - Stefanić, Martin
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1356
AB  - The strategy of combining polymers of natural and synthetic origin with inorganic components to use their unique synergistic effect for the development of the novel, sophisticated, and efficient 3D polymeric biomaterials, whose structure and properties mimic the extracellular matrix and simultaneously represent the suitable hydrogel platform for controlled drug release, is presented. The novel versatile 2-hydroxyethyl methacrylate/gelatin/alginate/iron(III) oxide based hydrogels are prepared by a simple but effective method-modified porogenation. Chemical composition, morphology, swelling capacity, porosity, mechanical properties, effects on cell viability, and in vitro degradation are tested to correlate the material's composition with the corresponding properties. The hydrogels show an interconnected porous microstructure, satisfactory mechanical strength, pH-sensitivity, and favorable curcumin release performances. The materials show good compatibility with healthy human fibroblast in cell culture judged by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, suggesting newly synthesized hydrogels as potentially a new generation of 3D biomaterials with tunable properties for versatile biomedical and pharmaceutical applications.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Macromolecular Chemistry and Physics
T1  - Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide
IS  - 20
VL  - 221
DO  - 10.1002/macp.202000186
ER  - 

@article{
author = "Babić, Marija M. and Vukomanović, Marija and Stefanić, Martin and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj.",
year = "2020",
abstract = "The strategy of combining polymers of natural and synthetic origin with inorganic components to use their unique synergistic effect for the development of the novel, sophisticated, and efficient 3D polymeric biomaterials, whose structure and properties mimic the extracellular matrix and simultaneously represent the suitable hydrogel platform for controlled drug release, is presented. The novel versatile 2-hydroxyethyl methacrylate/gelatin/alginate/iron(III) oxide based hydrogels are prepared by a simple but effective method-modified porogenation. Chemical composition, morphology, swelling capacity, porosity, mechanical properties, effects on cell viability, and in vitro degradation are tested to correlate the material's composition with the corresponding properties. The hydrogels show an interconnected porous microstructure, satisfactory mechanical strength, pH-sensitivity, and favorable curcumin release performances. The materials show good compatibility with healthy human fibroblast in cell culture judged by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, suggesting newly synthesized hydrogels as potentially a new generation of 3D biomaterials with tunable properties for versatile biomedical and pharmaceutical applications.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Macromolecular Chemistry and Physics",
title = "Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide",
number = "20",
volume = "221",
doi = "10.1002/macp.202000186"
}

Babić, M. M., Vukomanović, M., Stefanić, M., Nikodinović-Runić, J.,& Tomić, S. Lj.. (2020). Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide. in Macromolecular Chemistry and Physics
Wiley-V C H Verlag Gmbh, Weinheim., 221(20).
https://doi.org/10.1002/macp.202000186

Babić MM, Vukomanović M, Stefanić M, Nikodinović-Runić J, Tomić SL. Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide. in Macromolecular Chemistry and Physics. 2020;221(20).
doi:10.1002/macp.202000186 .

Babić, Marija M., Vukomanović, Marija, Stefanić, Martin, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj., "Controlled Curcumin Release from Hydrogel Scaffold Platform Based on 2-Hydroxyethyl Methacrylate/Gelatin/Alginate/Iron(III) Oxide" in Macromolecular Chemistry and Physics, 221, no. 20 (2020),
https://doi.org/10.1002/macp.202000186 . .

TY  - JOUR
AU  - Filipović, Vuk V.
AU  - Babić, Marija M.
AU  - Godevac, Dejan
AU  - Pavić, Aleksandar
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida Lj
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1256
AB  - New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it  gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Macromolecular Chemistry and Physics
T1  - In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release
IS  - 17
VL  - 220
DO  - 10.1002/macp.201900188
ER  - 

@article{
author = "Filipović, Vuk V. and Babić, Marija M. and Godevac, Dejan and Pavić, Aleksandar and Nikodinović-Runić, Jasmina and Tomić, Simonida Lj",
year = "2019",
abstract = "New (beta-aminoester) hydrogels (PBAE) based on di(ethylene glycol)diacrylate and glycine are successfully synthesized and characterized for the first time in this work. PBAE macromers are obtained using Michael addition. By changing the diacrylate/amine stoichiometric ratio, but maintaining it  gt 1, samples with different chemical structure containing acrylate end-groups are obtained. The hydrogels are synthesized from macromers utilizing free radical polymerization. Chemical structure of macromers and hydrogels is confirmed by proton nuclear magnetic resonance, and Fourier transform infra-red spectroscopy. Swelling and degradation rates in physiological pH range change notably with pH and monomer molar ratio, validating pH sensitivity and zwitterionic behavior, which can be finely tuned by changing any of these parameters. In vitro cytotoxicity and in vivo acute embryotoxicity in zebrafish (Danio rerio) performed to assess the biocompatibility of the novel hydrogel materials and their degradation products reveal that materials are nontoxic and biocompatible. The Cephalexin in vitro drug release study, at pH values 2.20, 5.50, and 7.40, demonstrates pH-sensitive delivery with the release profiles effectively controlled by pH and the hydrogel composition. PBAE hydrogels exhibit great potential for a variety of biomedical applications, including tissue regeneration and intelligent drug delivery systems.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Macromolecular Chemistry and Physics",
title = "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release",
number = "17",
volume = "220",
doi = "10.1002/macp.201900188"
}

Filipović, V. V., Babić, M. M., Godevac, D., Pavić, A., Nikodinović-Runić, J.,& Tomić, S. L.. (2019). In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics
Wiley-V C H Verlag Gmbh, Weinheim., 220(17).
https://doi.org/10.1002/macp.201900188

Filipović VV, Babić MM, Godevac D, Pavić A, Nikodinović-Runić J, Tomić SL. In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release. in Macromolecular Chemistry and Physics. 2019;220(17).
doi:10.1002/macp.201900188 .

Filipović, Vuk V., Babić, Marija M., Godevac, Dejan, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, Tomić, Simonida Lj, "In Vitro and In Vivo Biocompatibility of Novel Zwitterionic Poly(Beta Amino)Ester Hydrogels Based on Diacrylate and Glycine for Site-Specific Controlled Drug Release" in Macromolecular Chemistry and Physics, 220, no. 17 (2019),
https://doi.org/10.1002/macp.201900188 . .