TY  - JOUR
AU  - Stanković, Srdan
AU  - Morić, Ivana
AU  - Pavić, Aleksandar
AU  - Vasiljević, Branka
AU  - Johnson, D. Barrie
AU  - Cvetković, Vladica
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/712
AB  - An investigation of the microbial diversity in the extremely acidic, metal-rich Lake Robule was performed using culture-dependant and culture-independent (T-RFLP) methods. In addition, the ability of the indigenous bacteria from the lake water to leach copper from a mineral concentrate was tested. T-RFLP analysis revealed that the dominant bacteria in the lake water samples were the obligate heterotroph Acidiphilium cryptum (approximate to 50 % of the total bacteria) and the iron-oxidizing autotroph Leptospirillum ferrooxidans (approximate to 40 %) The iron/sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans was reported to be the most abundant bacteria in the Lake in an earlier study, but it was not detected in the present study using T-RFLP, although it was isolated on solid media and detected in enrichment (bioleaching) cultures. The presence of the two bacterial species detected by T-RFLP (L. ferrooxidans and A. cryptum) was also confirmed by cultivation on solid media. The presence and relative abundance of the bacteria inhabiting Lake Robule was explained by the physiological characteristics of the bacteria and the physico-chemical characteristics of the lake water.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)
EP  - 741
IS  - 6
SP  - 729
VL  - 79
DO  - 10.2298/JSC130605071S
ER  - 

@article{
author = "Stanković, Srdan and Morić, Ivana and Pavić, Aleksandar and Vasiljević, Branka and Johnson, D. Barrie and Cvetković, Vladica",
year = "2014",
abstract = "An investigation of the microbial diversity in the extremely acidic, metal-rich Lake Robule was performed using culture-dependant and culture-independent (T-RFLP) methods. In addition, the ability of the indigenous bacteria from the lake water to leach copper from a mineral concentrate was tested. T-RFLP analysis revealed that the dominant bacteria in the lake water samples were the obligate heterotroph Acidiphilium cryptum (approximate to 50 % of the total bacteria) and the iron-oxidizing autotroph Leptospirillum ferrooxidans (approximate to 40 %) The iron/sulfur-oxidizing autotroph Acidithiobacillus ferrooxidans was reported to be the most abundant bacteria in the Lake in an earlier study, but it was not detected in the present study using T-RFLP, although it was isolated on solid media and detected in enrichment (bioleaching) cultures. The presence of the two bacterial species detected by T-RFLP (L. ferrooxidans and A. cryptum) was also confirmed by cultivation on solid media. The presence and relative abundance of the bacteria inhabiting Lake Robule was explained by the physiological characteristics of the bacteria and the physico-chemical characteristics of the lake water.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)",
pages = "741-729",
number = "6",
volume = "79",
doi = "10.2298/JSC130605071S"
}

Stanković, S., Morić, I., Pavić, A., Vasiljević, B., Johnson, D. B.,& Cvetković, V.. (2014). Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia). in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 79(6), 729-741.
https://doi.org/10.2298/JSC130605071S

Stanković S, Morić I, Pavić A, Vasiljević B, Johnson DB, Cvetković V. Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia). in Journal of the Serbian Chemical Society. 2014;79(6):729-741.
doi:10.2298/JSC130605071S .

Stanković, Srdan, Morić, Ivana, Pavić, Aleksandar, Vasiljević, Branka, Johnson, D. Barrie, Cvetković, Vladica, "Investigation of the microbial diversity of an extremely acidic, metal-rich water body (Lake Robule, Bor, Serbia)" in Journal of the Serbian Chemical Society, 79, no. 6 (2014):729-741,
https://doi.org/10.2298/JSC130605071S . .