TY  - JOUR
AU  - Ilić-Tomić, Tatjana
AU  - Morić, Ivana
AU  - Conn, Graeme L.
AU  - Vasiljević, Branka
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/324
AB  - The aminoglycoside resistance genes sgm from Micromonospora zionensis and kgmB from Streptomyces tenebrarius were cloned into a yeast expression vector to test whether the encoded prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to G-418. Despite the detectable presence of mRNAs in yeast cells, neither G-418-resistant yeast transformants nor positive western blot signals were obtained. Neither methylase was capable of methylating 40S subunits despite very high conservation of the antibiotic rRNA binding sites. However, the results provide novel insight into the action of Sgm by showing that it methylates the same site as KgmB, i.e. G1405 in 16S rRNA.
PB  - Elsevier Science Bv, Amsterdam
T2  - Research in Microbiology
T1  - Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site
EP  - 662
IS  - 9-10
SP  - 658
VL  - 159
DO  - 10.1016/j.resmic.2008.09.006
ER  - 

@article{
author = "Ilić-Tomić, Tatjana and Morić, Ivana and Conn, Graeme L. and Vasiljević, Branka",
year = "2008",
abstract = "The aminoglycoside resistance genes sgm from Micromonospora zionensis and kgmB from Streptomyces tenebrarius were cloned into a yeast expression vector to test whether the encoded prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to G-418. Despite the detectable presence of mRNAs in yeast cells, neither G-418-resistant yeast transformants nor positive western blot signals were obtained. Neither methylase was capable of methylating 40S subunits despite very high conservation of the antibiotic rRNA binding sites. However, the results provide novel insight into the action of Sgm by showing that it methylates the same site as KgmB, i.e. G1405 in 16S rRNA.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Research in Microbiology",
title = "Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site",
pages = "662-658",
number = "9-10",
volume = "159",
doi = "10.1016/j.resmic.2008.09.006"
}

Ilić-Tomić, T., Morić, I., Conn, G. L.,& Vasiljević, B.. (2008). Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site. in Research in Microbiology
Elsevier Science Bv, Amsterdam., 159(9-10), 658-662.
https://doi.org/10.1016/j.resmic.2008.09.006

Ilić-Tomić T, Morić I, Conn GL, Vasiljević B. Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site. in Research in Microbiology. 2008;159(9-10):658-662.
doi:10.1016/j.resmic.2008.09.006 .

Ilić-Tomić, Tatjana, Morić, Ivana, Conn, Graeme L., Vasiljević, Branka, "Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site" in Research in Microbiology, 159, no. 9-10 (2008):658-662,
https://doi.org/10.1016/j.resmic.2008.09.006 . .