TY  - JOUR
AU  - Wagner, D.
AU  - Maser, J.
AU  - Morić, Ivana
AU  - Boechat, N.
AU  - Vogt, S.
AU  - Gicquel, B.
AU  - Lai, B.
AU  - Reyrat, J.-M.
AU  - Bermudez, L.
PY  - 2005
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/214
AB  - Pathogenic mycobacteria survive within phagosomes which are thought to represent a nutrient-restricted environment. Divalent cation transporters of the Nramp family in phagosomes and mycobacteria (Mramp) may compete for metals that are crucial for bacterial survival. The elemental concentrations in phagosomes of macrophages infected with wild-type Mycobacterium tuberculosis (M. tuberculosis strain H37Rv) and a M. tuberculosis Mramp knockout mutant (Mramp-KO), derived from a clinical isolate isogenic to the strain MT103, were compared. Time points of 1 and 24 h after infection of mouse peritoneal macrophages (bcgS) were compared in both cases. Increased concentrations of P, Ni and Zn and reduced Cl concentration in Mramp-KO after 1 h of infection were observed, compared to M. tuberculosis vacuoles. After 24 h of infection, significant differences in the P, Cl and Zn concentrations were still present. The Mramp-KO phagosome showed a significant increase of P, Ca, Mn, Fe and Zn concentrations between 1 and 24 h after infection, while the concentrations of K and Ni decreased. In the M. tuberculosis vacuole, the Fe concentration showed a similar increase, while the Cl concentration decreased. The fact that the concentration of several divalent cations increased in the Mramp-KO strain suggests that Mramp may have no impact on the import of these divalent cations into the mycobacterium, but may function as a cation efflux pump. The concordant increase of Fe concentrations within M. tuberculosis, as well as within the Mramp-KO vacuoles, implies that Mramp, in contrast to siderophores, might not be important for the attraction of Fe and its retention in phagosomes of unstimulated macrophages.
T2  - Microbiology-Sgm
T1  - Changes of the phagosomal elemental concentrations by Mycobacterium tuberculosis Mramp
EP  - 332
IS  - 1
SP  - 323
VL  - 151
DO  - 10.1099/mic.0.27213-0
ER  - 

@article{
author = "Wagner, D. and Maser, J. and Morić, Ivana and Boechat, N. and Vogt, S. and Gicquel, B. and Lai, B. and Reyrat, J.-M. and Bermudez, L.",
year = "2005",
abstract = "Pathogenic mycobacteria survive within phagosomes which are thought to represent a nutrient-restricted environment. Divalent cation transporters of the Nramp family in phagosomes and mycobacteria (Mramp) may compete for metals that are crucial for bacterial survival. The elemental concentrations in phagosomes of macrophages infected with wild-type Mycobacterium tuberculosis (M. tuberculosis strain H37Rv) and a M. tuberculosis Mramp knockout mutant (Mramp-KO), derived from a clinical isolate isogenic to the strain MT103, were compared. Time points of 1 and 24 h after infection of mouse peritoneal macrophages (bcgS) were compared in both cases. Increased concentrations of P, Ni and Zn and reduced Cl concentration in Mramp-KO after 1 h of infection were observed, compared to M. tuberculosis vacuoles. After 24 h of infection, significant differences in the P, Cl and Zn concentrations were still present. The Mramp-KO phagosome showed a significant increase of P, Ca, Mn, Fe and Zn concentrations between 1 and 24 h after infection, while the concentrations of K and Ni decreased. In the M. tuberculosis vacuole, the Fe concentration showed a similar increase, while the Cl concentration decreased. The fact that the concentration of several divalent cations increased in the Mramp-KO strain suggests that Mramp may have no impact on the import of these divalent cations into the mycobacterium, but may function as a cation efflux pump. The concordant increase of Fe concentrations within M. tuberculosis, as well as within the Mramp-KO vacuoles, implies that Mramp, in contrast to siderophores, might not be important for the attraction of Fe and its retention in phagosomes of unstimulated macrophages.",
journal = "Microbiology-Sgm",
title = "Changes of the phagosomal elemental concentrations by Mycobacterium tuberculosis Mramp",
pages = "332-323",
number = "1",
volume = "151",
doi = "10.1099/mic.0.27213-0"
}

Wagner, D., Maser, J., Morić, I., Boechat, N., Vogt, S., Gicquel, B., Lai, B., Reyrat, J.-M.,& Bermudez, L.. (2005). Changes of the phagosomal elemental concentrations by Mycobacterium tuberculosis Mramp. in Microbiology-Sgm, 151(1), 323-332.
https://doi.org/10.1099/mic.0.27213-0

Wagner D, Maser J, Morić I, Boechat N, Vogt S, Gicquel B, Lai B, Reyrat J, Bermudez L. Changes of the phagosomal elemental concentrations by Mycobacterium tuberculosis Mramp. in Microbiology-Sgm. 2005;151(1):323-332.
doi:10.1099/mic.0.27213-0 .

Wagner, D., Maser, J., Morić, Ivana, Boechat, N., Vogt, S., Gicquel, B., Lai, B., Reyrat, J.-M., Bermudez, L., "Changes of the phagosomal elemental concentrations by Mycobacterium tuberculosis Mramp" in Microbiology-Sgm, 151, no. 1 (2005):323-332,
https://doi.org/10.1099/mic.0.27213-0 . .