TY  - JOUR
AU  - Stanisić, Ljubodrag
AU  - Aleksić, Jelena M.
AU  - Dimitrijević, Vladimir
AU  - Kovacević, Branislav
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1372
AB  - The dominant donkey breed in the Balkans is the mid-sized Balkan donkey with a grey to chocolate coat color. Local breeders from Serbia, however, still maintain a few larger individuals of a lighter coat color, named Banat donkey, and speculate that they are descendants of a Spanish donkey heard that had been transferred to the Banat region by the Hapsburg Queen Maria Theresa in the XVIII century for a specific purpose, to work in local vineyards. We have previously found a unique nuclear gene-pool and a prevalence of mitochondrial Clade 2 haplotypes in several such animals. In this study, we: (i) perform a comparative analysis of 18 morphological traits of the Banat donkey (seven individuals), Balkan donkey (53 individuals from two sub-populations of this breed) and the potential hybrids (eight individuals), and demonstrate the morphological distinctiveness of the Banat donkey, highlighting the diagnostic traits for distinguishing the breed: hip height, croup width, body length and chest depth; (ii) reanalyse published nuclear microsatellite data for these groups, and reveal that, although severely depopulated, the genetically distinct Banat donkey is not severely affected by the loss of genetic diversity and inbreeding; (iii) demonstrate that previously published Banat donkey mitochondrial haplotypes, analyzed genealogically together with those reported in ancient and modern individuals from Spain, Italy, Turkey, Cyprus and Africa, are shared with three Spanish breeds and individuals belonging to Amiata and some other Italian breeds. A unique morphological feature present in Banat and Somali wild donkeys, but also in Amiata donkeys, black stripes on legs, suggests that the origin of Clade 2 donkeys may be much more complex than previously thought. Actions to preserve the Banat donkey, a valuable but critically endangered genetic resource ( lt 100 individuals), are urgent.
PB  - PEERJ Inc, London
T2  - PEERJ
T1  - Banat donkey, a neglected donkey breed from the central Balkans (Serbia)
VL  - 8
DO  - 10.7717/peerj.8598
ER  - 

@article{
author = "Stanisić, Ljubodrag and Aleksić, Jelena M. and Dimitrijević, Vladimir and Kovacević, Branislav and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2020",
abstract = "The dominant donkey breed in the Balkans is the mid-sized Balkan donkey with a grey to chocolate coat color. Local breeders from Serbia, however, still maintain a few larger individuals of a lighter coat color, named Banat donkey, and speculate that they are descendants of a Spanish donkey heard that had been transferred to the Banat region by the Hapsburg Queen Maria Theresa in the XVIII century for a specific purpose, to work in local vineyards. We have previously found a unique nuclear gene-pool and a prevalence of mitochondrial Clade 2 haplotypes in several such animals. In this study, we: (i) perform a comparative analysis of 18 morphological traits of the Banat donkey (seven individuals), Balkan donkey (53 individuals from two sub-populations of this breed) and the potential hybrids (eight individuals), and demonstrate the morphological distinctiveness of the Banat donkey, highlighting the diagnostic traits for distinguishing the breed: hip height, croup width, body length and chest depth; (ii) reanalyse published nuclear microsatellite data for these groups, and reveal that, although severely depopulated, the genetically distinct Banat donkey is not severely affected by the loss of genetic diversity and inbreeding; (iii) demonstrate that previously published Banat donkey mitochondrial haplotypes, analyzed genealogically together with those reported in ancient and modern individuals from Spain, Italy, Turkey, Cyprus and Africa, are shared with three Spanish breeds and individuals belonging to Amiata and some other Italian breeds. A unique morphological feature present in Banat and Somali wild donkeys, but also in Amiata donkeys, black stripes on legs, suggests that the origin of Clade 2 donkeys may be much more complex than previously thought. Actions to preserve the Banat donkey, a valuable but critically endangered genetic resource ( lt 100 individuals), are urgent.",
publisher = "PEERJ Inc, London",
journal = "PEERJ",
title = "Banat donkey, a neglected donkey breed from the central Balkans (Serbia)",
volume = "8",
doi = "10.7717/peerj.8598"
}

Stanisić, L., Aleksić, J. M., Dimitrijević, V., Kovacević, B., Stevanović, J.,& Stanimirović, Z.. (2020). Banat donkey, a neglected donkey breed from the central Balkans (Serbia). in PEERJ
PEERJ Inc, London., 8.
https://doi.org/10.7717/peerj.8598

Stanisić L, Aleksić JM, Dimitrijević V, Kovacević B, Stevanović J, Stanimirović Z. Banat donkey, a neglected donkey breed from the central Balkans (Serbia). in PEERJ. 2020;8.
doi:10.7717/peerj.8598 .

Stanisić, Ljubodrag, Aleksić, Jelena M., Dimitrijević, Vladimir, Kovacević, Branislav, Stevanović, Jevrosima, Stanimirović, Zoran, "Banat donkey, a neglected donkey breed from the central Balkans (Serbia)" in PEERJ, 8 (2020),
https://doi.org/10.7717/peerj.8598 . .

TY  - JOUR
AU  - Dimitrijević, Vladimir
AU  - Savić, Mila
AU  - Tarić, Elmin
AU  - Stanisić, Ljubodrag
AU  - Stanimirović, Zoran
AU  - Tabaković, Aleksandar
AU  - Aleksić, Jelena M.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1358
AB  - Yugoslavian Shepherd Dog - Sharplanina (YSD) is a livestock guard dog from the Western Balkans present in this region over a long time, but recognized by the Federation Cynologique Internationale as a distinct breed as late as 1957. However, the information regarding the origin and the size of the breed's foundation stock is still lacking. In order to contribute towards better understanding of the genetic make-up of the YSD and its foundation stock, we re-analyzed previously generated genetic profiles of 94 registered YSD dogs assessed with nine nuclear microsatellites. Studied individuals comprised 90 unrelated dogs and two pairs of full-sibs, sampled at four sampling sites: three dog shows and at a military training centre for dogs in Serbia. We supported earlier findings on high levels of genetic diversity in YSD (H-E=0.728 +/- 0.027) and lack of inbreeding, and revealed substructure of the breed because we found two distinct gene pools in the Bayesian clustering analysis, indicated also by the excess of homozygotes (i.e., Wahlund effect) and outcomes of other analyses: linkage disequilibrium tests, Neighbour-Joining tree, principal coordinates and two-dimensional scaling analyses. The two gene pools were almost equally represented at each sampling site. One gene pool was composed of individuals with high genetic integrity, while the other gene pool was characterized with admixed ancestry, developed possibly via hybridization with native breeding stock outside the registry system, other breeds, such as the Caucasian Shepherd, and/or individuals admixed with wolves. Thus, we demonstrate rather complex and diverse ancestry implying a genetically heterogeneous foundation stock of the YSD.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Genetic characterization of the Yugoslavian shepherd dog - sharplanina, a livestock guard dog from the Western Balkans
EP  - 345
IS  - 3
SP  - 329
VL  - 70
DO  - 10.2478/acve-2020-0025
ER  - 

@article{
author = "Dimitrijević, Vladimir and Savić, Mila and Tarić, Elmin and Stanisić, Ljubodrag and Stanimirović, Zoran and Tabaković, Aleksandar and Aleksić, Jelena M.",
year = "2020",
abstract = "Yugoslavian Shepherd Dog - Sharplanina (YSD) is a livestock guard dog from the Western Balkans present in this region over a long time, but recognized by the Federation Cynologique Internationale as a distinct breed as late as 1957. However, the information regarding the origin and the size of the breed's foundation stock is still lacking. In order to contribute towards better understanding of the genetic make-up of the YSD and its foundation stock, we re-analyzed previously generated genetic profiles of 94 registered YSD dogs assessed with nine nuclear microsatellites. Studied individuals comprised 90 unrelated dogs and two pairs of full-sibs, sampled at four sampling sites: three dog shows and at a military training centre for dogs in Serbia. We supported earlier findings on high levels of genetic diversity in YSD (H-E=0.728 +/- 0.027) and lack of inbreeding, and revealed substructure of the breed because we found two distinct gene pools in the Bayesian clustering analysis, indicated also by the excess of homozygotes (i.e., Wahlund effect) and outcomes of other analyses: linkage disequilibrium tests, Neighbour-Joining tree, principal coordinates and two-dimensional scaling analyses. The two gene pools were almost equally represented at each sampling site. One gene pool was composed of individuals with high genetic integrity, while the other gene pool was characterized with admixed ancestry, developed possibly via hybridization with native breeding stock outside the registry system, other breeds, such as the Caucasian Shepherd, and/or individuals admixed with wolves. Thus, we demonstrate rather complex and diverse ancestry implying a genetically heterogeneous foundation stock of the YSD.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Genetic characterization of the Yugoslavian shepherd dog - sharplanina, a livestock guard dog from the Western Balkans",
pages = "345-329",
number = "3",
volume = "70",
doi = "10.2478/acve-2020-0025"
}

Dimitrijević, V., Savić, M., Tarić, E., Stanisić, L., Stanimirović, Z., Tabaković, A.,& Aleksić, J. M.. (2020). Genetic characterization of the Yugoslavian shepherd dog - sharplanina, a livestock guard dog from the Western Balkans. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3), 329-345.
https://doi.org/10.2478/acve-2020-0025

Dimitrijević V, Savić M, Tarić E, Stanisić L, Stanimirović Z, Tabaković A, Aleksić JM. Genetic characterization of the Yugoslavian shepherd dog - sharplanina, a livestock guard dog from the Western Balkans. in Acta Veterinaria-Beograd. 2020;70(3):329-345.
doi:10.2478/acve-2020-0025 .

Dimitrijević, Vladimir, Savić, Mila, Tarić, Elmin, Stanisić, Ljubodrag, Stanimirović, Zoran, Tabaković, Aleksandar, Aleksić, Jelena M., "Genetic characterization of the Yugoslavian shepherd dog - sharplanina, a livestock guard dog from the Western Balkans" in Acta Veterinaria-Beograd, 70, no. 3 (2020):329-345,
https://doi.org/10.2478/acve-2020-0025 . .

TY  - JOUR
AU  - Cirković, Dragan
AU  - Stevanović, Jevrosima
AU  - Glavinić, Uros
AU  - Aleksić, Nevenka
AU  - Đurić, Spomenka
AU  - Aleksić, Jelena M.
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1130
AB  - Protection of honey bees is of great economic importance because of their role in pollination. Crucial steps towards this goal are epidemiological surveys of pathogens connected with honey bee losses. In this study deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and sacbrood virus (SBV) were investigated in colonies of different strength located in five regions of Serbia. The relationship between colony strength and virus occurrence/infection intensity were assessed as well as the genetic relationship between virus sequences from Serbia and worldwide. Real-time RT-PCR analyses detected at least one virus in 87.33% of colonies. Single infection was found in 28.67% colonies (21.33%, 4.00%, 2.67% and 0.67% in cases of DWV, ABPV, SBV and CBPV, respectively). In the majority of colonies (58.66%) more than one virus was found. The most prevalent was DWV (74%), followed by ABPV, SBV and CBPV (49.30%, 24.00% and 6.70%, respectively). Except for DWV, the prevalence of the remaining three viruses significantly varied between the regions. No significant differences were found between colony strength and either (i) the prevalence of DWV, ABPV, SBV, CBPV and their combinations, or (ii) DWV infection levels. The sequences of honey bee viruses obtained from bees in Serbia were 93-99% identical with those deposited in GenBank.
PB  - PEERJ Inc, London
T2  - PEERJ
T1  - Honey bee viruses in Serbian colonies of different strength
VL  - 6
DO  - 10.7717/peerj.5887
ER  - 

@article{
author = "Cirković, Dragan and Stevanović, Jevrosima and Glavinić, Uros and Aleksić, Nevenka and Đurić, Spomenka and Aleksić, Jelena M. and Stanimirović, Zoran",
year = "2018",
abstract = "Protection of honey bees is of great economic importance because of their role in pollination. Crucial steps towards this goal are epidemiological surveys of pathogens connected with honey bee losses. In this study deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and sacbrood virus (SBV) were investigated in colonies of different strength located in five regions of Serbia. The relationship between colony strength and virus occurrence/infection intensity were assessed as well as the genetic relationship between virus sequences from Serbia and worldwide. Real-time RT-PCR analyses detected at least one virus in 87.33% of colonies. Single infection was found in 28.67% colonies (21.33%, 4.00%, 2.67% and 0.67% in cases of DWV, ABPV, SBV and CBPV, respectively). In the majority of colonies (58.66%) more than one virus was found. The most prevalent was DWV (74%), followed by ABPV, SBV and CBPV (49.30%, 24.00% and 6.70%, respectively). Except for DWV, the prevalence of the remaining three viruses significantly varied between the regions. No significant differences were found between colony strength and either (i) the prevalence of DWV, ABPV, SBV, CBPV and their combinations, or (ii) DWV infection levels. The sequences of honey bee viruses obtained from bees in Serbia were 93-99% identical with those deposited in GenBank.",
publisher = "PEERJ Inc, London",
journal = "PEERJ",
title = "Honey bee viruses in Serbian colonies of different strength",
volume = "6",
doi = "10.7717/peerj.5887"
}

Cirković, D., Stevanović, J., Glavinić, U., Aleksić, N., Đurić, S., Aleksić, J. M.,& Stanimirović, Z.. (2018). Honey bee viruses in Serbian colonies of different strength. in PEERJ
PEERJ Inc, London., 6.
https://doi.org/10.7717/peerj.5887

Cirković D, Stevanović J, Glavinić U, Aleksić N, Đurić S, Aleksić JM, Stanimirović Z. Honey bee viruses in Serbian colonies of different strength. in PEERJ. 2018;6.
doi:10.7717/peerj.5887 .

Cirković, Dragan, Stevanović, Jevrosima, Glavinić, Uros, Aleksić, Nevenka, Đurić, Spomenka, Aleksić, Jelena M., Stanimirović, Zoran, "Honey bee viruses in Serbian colonies of different strength" in PEERJ, 6 (2018),
https://doi.org/10.7717/peerj.5887 . .

TY  - JOUR
AU  - Glavinić, Uros
AU  - Stanković, Biljana
AU  - Drasković, Vladimir
AU  - Stevanović, Jevrosima
AU  - Petrović, Tamas
AU  - Lakić, Nada
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1040
AB  - Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex "BEEWELL AminoPlus" to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p lt 0.01 or p lt 0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p lt 0.01 or p lt 0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae
IS  - 11
VL  - 12
DO  - 10.1371/journal.pone.0187726
ER  - 

@article{
author = "Glavinić, Uros and Stanković, Biljana and Drasković, Vladimir and Stevanović, Jevrosima and Petrović, Tamas and Lakić, Nada and Stanimirović, Zoran",
year = "2017",
abstract = "Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex "BEEWELL AminoPlus" to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p lt 0.01 or p lt 0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p lt 0.01 or p lt 0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae",
number = "11",
volume = "12",
doi = "10.1371/journal.pone.0187726"
}

Glavinić, U., Stanković, B., Drasković, V., Stevanović, J., Petrović, T., Lakić, N.,& Stanimirović, Z.. (2017). Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. in PLoS One
Public Library Science, San Francisco., 12(11).
https://doi.org/10.1371/journal.pone.0187726

Glavinić U, Stanković B, Drasković V, Stevanović J, Petrović T, Lakić N, Stanimirović Z. Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. in PLoS One. 2017;12(11).
doi:10.1371/journal.pone.0187726 .

Glavinić, Uros, Stanković, Biljana, Drasković, Vladimir, Stevanović, Jevrosima, Petrović, Tamas, Lakić, Nada, Stanimirović, Zoran, "Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae" in PLoS One, 12, no. 11 (2017),
https://doi.org/10.1371/journal.pone.0187726 . .

TY  - JOUR
AU  - Stanisić, L. J.
AU  - Aleksić, Jelena M.
AU  - Dimitrijević, Vladimir
AU  - Simeunović, P.
AU  - Glavinić, U.
AU  - Stevanović, J.
AU  - Stanimirović, Z.
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/994
AB  - The Balkan donkey (Equus asinus L.) is commonly regarded as a large-sized, unselected, unstructured and traditionally managed donkey breed. We assessed the current genetic status of the three largest E. asinus populations in the central Balkans (Serbia) by analysing the variability of nuclear microsatellites and the mitochondrial (mtDNA) control region of 77 and 49 individuals respectively. We further analysed our mtDNA dataset along with 209 published mtDNA sequences of ancient and modern individuals from 19 European and African populations to provide new insights into the origin and the history of the Balkan donkey. Serbian donkey populations are highly genetically diverse at both the nuclear and mtDNA levels despite severe population decline. Traditional Balkan donkeys in Serbia are rather heterogeneous; we found two groups of individuals with similar phenotypic features, somewhat distinct nuclear backgrounds and different proportions of mtDNA haplotypes belonging to matrilineal Clades 1 and 2. Another group, characterized by larger body size, different coat colour, distinct nuclear gene pool and predominantly Clade 2 haplotypes, was delineated as the Banat donkey breed. The maternal landscape of the large Balkan donkey population is highly heterogeneous and more complex than previously thought. Given the two independent domestication events in donkeys, multiple waves of introductions into the Balkans from Greece are hypothesized. Clade 2 donkeys probably appeared in Greece prior to those belonging to Clade 1, whereas expansion and diversification of Clade 1 donkeys within the Balkans predated that of Clade 2 donkeys.
PB  - Wiley, Hoboken
T2  - Animal Genetics
T1  - New insights into the origin and the genetic status of the Balkan donkey from Serbia
EP  - 590
IS  - 5
SP  - 580
VL  - 48
DO  - 10.1111/age.12589
ER  - 

@article{
author = "Stanisić, L. J. and Aleksić, Jelena M. and Dimitrijević, Vladimir and Simeunović, P. and Glavinić, U. and Stevanović, J. and Stanimirović, Z.",
year = "2017",
abstract = "The Balkan donkey (Equus asinus L.) is commonly regarded as a large-sized, unselected, unstructured and traditionally managed donkey breed. We assessed the current genetic status of the three largest E. asinus populations in the central Balkans (Serbia) by analysing the variability of nuclear microsatellites and the mitochondrial (mtDNA) control region of 77 and 49 individuals respectively. We further analysed our mtDNA dataset along with 209 published mtDNA sequences of ancient and modern individuals from 19 European and African populations to provide new insights into the origin and the history of the Balkan donkey. Serbian donkey populations are highly genetically diverse at both the nuclear and mtDNA levels despite severe population decline. Traditional Balkan donkeys in Serbia are rather heterogeneous; we found two groups of individuals with similar phenotypic features, somewhat distinct nuclear backgrounds and different proportions of mtDNA haplotypes belonging to matrilineal Clades 1 and 2. Another group, characterized by larger body size, different coat colour, distinct nuclear gene pool and predominantly Clade 2 haplotypes, was delineated as the Banat donkey breed. The maternal landscape of the large Balkan donkey population is highly heterogeneous and more complex than previously thought. Given the two independent domestication events in donkeys, multiple waves of introductions into the Balkans from Greece are hypothesized. Clade 2 donkeys probably appeared in Greece prior to those belonging to Clade 1, whereas expansion and diversification of Clade 1 donkeys within the Balkans predated that of Clade 2 donkeys.",
publisher = "Wiley, Hoboken",
journal = "Animal Genetics",
title = "New insights into the origin and the genetic status of the Balkan donkey from Serbia",
pages = "590-580",
number = "5",
volume = "48",
doi = "10.1111/age.12589"
}

Stanisić, L. J., Aleksić, J. M., Dimitrijević, V., Simeunović, P., Glavinić, U., Stevanović, J.,& Stanimirović, Z.. (2017). New insights into the origin and the genetic status of the Balkan donkey from Serbia. in Animal Genetics
Wiley, Hoboken., 48(5), 580-590.
https://doi.org/10.1111/age.12589

Stanisić LJ, Aleksić JM, Dimitrijević V, Simeunović P, Glavinić U, Stevanović J, Stanimirović Z. New insights into the origin and the genetic status of the Balkan donkey from Serbia. in Animal Genetics. 2017;48(5):580-590.
doi:10.1111/age.12589 .

Stanisić, L. J., Aleksić, Jelena M., Dimitrijević, Vladimir, Simeunović, P., Glavinić, U., Stevanović, J., Stanimirović, Z., "New insights into the origin and the genetic status of the Balkan donkey from Serbia" in Animal Genetics, 48, no. 5 (2017):580-590,
https://doi.org/10.1111/age.12589 . .