TY  - JOUR
AU  - Petrović, Isidora
AU  - Nikčević, Gordana
AU  - Zarić, Jelena
AU  - Ruegg, Curzio
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/431
AB  - Angiogenesis, the process of generating new blood vessels, is essential to embryonic development, organ formation, tissue regeneration and remodeling, reproduction and wound healing. Also, it plays an important role in many pathological conditions, including chronic inflammation and cancer. Angiogenesis is regulated by a complex interplay of growth factors, inflammatory mediators, adhesion molecules, morphogens and guidance molecules. Transcription factor SOX18 is transiently expressed in nascent endothelial cells during embryonic development and postnatal angiogenesis, but little is known about signaling pathways controlling its expression. The aim of this study was to investigate whether pro-angiogenic molecules and pharmacological inhibitors of angiogenesis modulate SOX18 expression in endothelial cells. Therefore, we treated human umbilical vein endothelial cells (HUVEC) with angiogenic factors, extracellular matrix proteins, inflammatory cytokines and nonsteroidal anti-inflammatory drugs (NSAID) and monitored SOX18 expression. We have observed that the angiogenic factor VEGF and the inflammatory cytokine TNF increase, while the NSAID ibuprofen and NS398 decrease the SOX18 protein level. These results for the first time demonstrate that SOX18 expression is modulated by factors and drugs known to positively or negatively regulate angiogenesis. This opens the possibility of pharmacological manipulation of SOX18 gene expression in endothelial cells to stimulate or inhibit angiogenesis.
PB  - Versita, Warsaw
T2  - Central European Journal of Biology
T1  - VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC
EP  - 434
IS  - 4
SP  - 427
VL  - 5
DO  - 10.2478/s11535-010-0031-3
ER  - 

@article{
author = "Petrović, Isidora and Nikčević, Gordana and Zarić, Jelena and Ruegg, Curzio and Stevanović, Milena",
year = "2010",
abstract = "Angiogenesis, the process of generating new blood vessels, is essential to embryonic development, organ formation, tissue regeneration and remodeling, reproduction and wound healing. Also, it plays an important role in many pathological conditions, including chronic inflammation and cancer. Angiogenesis is regulated by a complex interplay of growth factors, inflammatory mediators, adhesion molecules, morphogens and guidance molecules. Transcription factor SOX18 is transiently expressed in nascent endothelial cells during embryonic development and postnatal angiogenesis, but little is known about signaling pathways controlling its expression. The aim of this study was to investigate whether pro-angiogenic molecules and pharmacological inhibitors of angiogenesis modulate SOX18 expression in endothelial cells. Therefore, we treated human umbilical vein endothelial cells (HUVEC) with angiogenic factors, extracellular matrix proteins, inflammatory cytokines and nonsteroidal anti-inflammatory drugs (NSAID) and monitored SOX18 expression. We have observed that the angiogenic factor VEGF and the inflammatory cytokine TNF increase, while the NSAID ibuprofen and NS398 decrease the SOX18 protein level. These results for the first time demonstrate that SOX18 expression is modulated by factors and drugs known to positively or negatively regulate angiogenesis. This opens the possibility of pharmacological manipulation of SOX18 gene expression in endothelial cells to stimulate or inhibit angiogenesis.",
publisher = "Versita, Warsaw",
journal = "Central European Journal of Biology",
title = "VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC",
pages = "434-427",
number = "4",
volume = "5",
doi = "10.2478/s11535-010-0031-3"
}

Petrović, I., Nikčević, G., Zarić, J., Ruegg, C.,& Stevanović, M.. (2010). VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC. in Central European Journal of Biology
Versita, Warsaw., 5(4), 427-434.
https://doi.org/10.2478/s11535-010-0031-3

Petrović I, Nikčević G, Zarić J, Ruegg C, Stevanović M. VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC. in Central European Journal of Biology. 2010;5(4):427-434.
doi:10.2478/s11535-010-0031-3 .

Petrović, Isidora, Nikčević, Gordana, Zarić, Jelena, Ruegg, Curzio, Stevanović, Milena, "VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC" in Central European Journal of Biology, 5, no. 4 (2010):427-434,
https://doi.org/10.2478/s11535-010-0031-3 . .