TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Dias, Maria Ines
AU  - Zengin, Gokhan
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Rajcević, Nemanja
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1574
AB  - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
PB  - EXCLI Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line
EP  - 399
SP  - 387
VL  - 21
DO  - 10.17179/excli2021-4487
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Ines and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajcević, Nemanja and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2022",
abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.",
publisher = "EXCLI Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line",
pages = "399-387",
volume = "21",
doi = "10.17179/excli2021-4487"
}

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajcević, N., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2022). Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal
EXCLI Journal Managing Office, Dortmund., 21, 387-399.
https://doi.org/10.17179/excli2021-4487

Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajcević N, Stevanović M, Ferreira ICFR, Soković M. Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal. 2022;21:387-399.
doi:10.17179/excli2021-4487 .

Stojković, Dejan, Drakulić, Danijela, Dias, Maria Ines, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajcević, Nemanja, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line" in EXCLI Journal, 21 (2022):387-399,
https://doi.org/10.17179/excli2021-4487 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Dias, Maria Ines
AU  - Drakulić, Danijela
AU  - Barros, Lillian
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina D.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1374
AB  - Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
PB  - MDPI, Basel
T2  - Pharmaceuticals
T1  - Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells
IS  - 4
VL  - 13
DO  - 10.3390/ph13040078
ER  - 

@article{
author = "Stojković, Dejan and Dias, Maria Ines and Drakulić, Danijela and Barros, Lillian and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina D.",
year = "2020",
abstract = "Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.",
publisher = "MDPI, Basel",
journal = "Pharmaceuticals",
title = "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells",
number = "4",
volume = "13",
doi = "10.3390/ph13040078"
}

Stojković, D., Dias, M. I., Drakulić, D., Barros, L., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M. D.. (2020). Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells. in Pharmaceuticals
MDPI, Basel., 13(4).
https://doi.org/10.3390/ph13040078

Stojković D, Dias MI, Drakulić D, Barros L, Stevanović M, Ferreira ICFR, Soković MD. Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells. in Pharmaceuticals. 2020;13(4).
doi:10.3390/ph13040078 .

Stojković, Dejan, Dias, Maria Ines, Drakulić, Danijela, Barros, Lillian, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina D., "Methanolic Extract of the Herb Ononis spinosa L. Is an Antifungal Agent with no Cytotoxicity to Primary Human Cells" in Pharmaceuticals, 13, no. 4 (2020),
https://doi.org/10.3390/ph13040078 . .