TY  - JOUR
AU  - Lazić, Andrijana
AU  - Balint, Vanda
AU  - Stanisavljević Ninković, Danijela
AU  - Perić, Mina
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1602
AB  - Astrocytes, as the most abundant glial cells in the central nervous system, are tightly integrated into neural networks and participate in numerous aspects of brain physiology and pathology. They are the main homeostatic cells in the central nervous system, and the loss of astrocyte physiological functions and/or gain of pro-inflammatory functions, due to their reactivation or cellular senescence, can have profound impacts on the surrounding microenvironment with pathological outcomes. Although the importance of astrocytes is generally recognized, and both senescence and reactive astrogliosis have been extensively reviewed independently, there are only a few comparative overviews of these complex processes. In this review, we summarize the latest data regarding astrocyte reactivation and senescence, and outline similarities and differences between these phenotypes from morphological, functional, and molecular points of view. A special focus has been given to neurodegenerative diseases, where these phenotypic alternations of astrocytes are significantly implicated. We also summarize current perspectives regarding new advances in model systems based on astrocytes as well as data pointing to these glial cells as potential therapeutic targets.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Reactive and Senescent Astroglial Phenotypes as Hallmarks of Brain Pathologies
IS  - 9
VL  - 23
DO  - 10.3390/ijms23094995
ER  - 

@article{
author = "Lazić, Andrijana and Balint, Vanda and Stanisavljević Ninković, Danijela and Perić, Mina and Stevanović, Milena",
year = "2022",
abstract = "Astrocytes, as the most abundant glial cells in the central nervous system, are tightly integrated into neural networks and participate in numerous aspects of brain physiology and pathology. They are the main homeostatic cells in the central nervous system, and the loss of astrocyte physiological functions and/or gain of pro-inflammatory functions, due to their reactivation or cellular senescence, can have profound impacts on the surrounding microenvironment with pathological outcomes. Although the importance of astrocytes is generally recognized, and both senescence and reactive astrogliosis have been extensively reviewed independently, there are only a few comparative overviews of these complex processes. In this review, we summarize the latest data regarding astrocyte reactivation and senescence, and outline similarities and differences between these phenotypes from morphological, functional, and molecular points of view. A special focus has been given to neurodegenerative diseases, where these phenotypic alternations of astrocytes are significantly implicated. We also summarize current perspectives regarding new advances in model systems based on astrocytes as well as data pointing to these glial cells as potential therapeutic targets.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Reactive and Senescent Astroglial Phenotypes as Hallmarks of Brain Pathologies",
number = "9",
volume = "23",
doi = "10.3390/ijms23094995"
}

Lazić, A., Balint, V., Stanisavljević Ninković, D., Perić, M.,& Stevanović, M.. (2022). Reactive and Senescent Astroglial Phenotypes as Hallmarks of Brain Pathologies. in International Journal of Molecular Sciences
MDPI, Basel., 23(9).
https://doi.org/10.3390/ijms23094995

Lazić A, Balint V, Stanisavljević Ninković D, Perić M, Stevanović M. Reactive and Senescent Astroglial Phenotypes as Hallmarks of Brain Pathologies. in International Journal of Molecular Sciences. 2022;23(9).
doi:10.3390/ijms23094995 .

Lazić, Andrijana, Balint, Vanda, Stanisavljević Ninković, Danijela, Perić, Mina, Stevanović, Milena, "Reactive and Senescent Astroglial Phenotypes as Hallmarks of Brain Pathologies" in International Journal of Molecular Sciences, 23, no. 9 (2022),
https://doi.org/10.3390/ijms23094995 . .

TY  - JOUR
AU  - Balint, Vanda
AU  - Stanisavljević Ninković, Danijela
AU  - Anastasov, Nataša
AU  - Lazić, Stefan
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1472
AB  - Astrocytes are the main homeostatic cells in the central nervous system (CNS) that provide mechanical, metabolic, and trophic support to neurons. Disruption of their physiological role or acquisition of senescence-associated phenotype can contribute to the CNS dysfunction and pathology. However, molecular mechanisms underlying the complex physiology of astrocytes are explored insufficiently. Recent studies have shown that miRNAs are involved in the regulation of astrocyte function through different mechanisms. Although miR-21 has been reported as an astrocytic miRNA with an important role in astrogliosis, no link between this miRNA and cellular senescence of astrocytes has been identified. To address the role of miR-21 in astrocytes, with special focus on cellular senescence, we used NT2/A (astrocytes derived from NT2/D1 cells). Downregulation of miR-21 expression in both immature and mature NT2/A by the antisense technology induced the arrest of cell growth and premature cellular senescence, as indicated by senescence hallmarks such as increased expression of cell cycle inhibitors p21 and p53 and augmented senescence-associated beta-galactosidase activity. Additionally, in silico analysis predicted many of the genes, previously shown to be upregulated in astrocytes with the irradiation-induced senescence, as miR-21 targets. Taken together, our results point to miR-21 as a potential regulator of astrocyte senescence. To the best of our knowledge, these are the first data showing the link between miR-21 and cellular senescence of astrocytes. Since senescent astrocytes are associated with different CNS pathologies, development of novel therapeutic strategies based on miRNA manipulation could prevent senescence and may improve the physiological outcome.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Biochemistry-Moscow
T1  - Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes
EP  - 1445
IS  - 11
SP  - 1434
VL  - 86
DO  - 10.1134/S0006297921110079
ER  - 

@article{
author = "Balint, Vanda and Stanisavljević Ninković, Danijela and Anastasov, Nataša and Lazić, Stefan and Kovačević Grujičić, Nataša and Stevanović, Milena and Lazić, Andrijana",
year = "2021",
abstract = "Astrocytes are the main homeostatic cells in the central nervous system (CNS) that provide mechanical, metabolic, and trophic support to neurons. Disruption of their physiological role or acquisition of senescence-associated phenotype can contribute to the CNS dysfunction and pathology. However, molecular mechanisms underlying the complex physiology of astrocytes are explored insufficiently. Recent studies have shown that miRNAs are involved in the regulation of astrocyte function through different mechanisms. Although miR-21 has been reported as an astrocytic miRNA with an important role in astrogliosis, no link between this miRNA and cellular senescence of astrocytes has been identified. To address the role of miR-21 in astrocytes, with special focus on cellular senescence, we used NT2/A (astrocytes derived from NT2/D1 cells). Downregulation of miR-21 expression in both immature and mature NT2/A by the antisense technology induced the arrest of cell growth and premature cellular senescence, as indicated by senescence hallmarks such as increased expression of cell cycle inhibitors p21 and p53 and augmented senescence-associated beta-galactosidase activity. Additionally, in silico analysis predicted many of the genes, previously shown to be upregulated in astrocytes with the irradiation-induced senescence, as miR-21 targets. Taken together, our results point to miR-21 as a potential regulator of astrocyte senescence. To the best of our knowledge, these are the first data showing the link between miR-21 and cellular senescence of astrocytes. Since senescent astrocytes are associated with different CNS pathologies, development of novel therapeutic strategies based on miRNA manipulation could prevent senescence and may improve the physiological outcome.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Biochemistry-Moscow",
title = "Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes",
pages = "1445-1434",
number = "11",
volume = "86",
doi = "10.1134/S0006297921110079"
}

Balint, V., Stanisavljević Ninković, D., Anastasov, N., Lazić, S., Kovačević Grujičić, N., Stevanović, M.,& Lazić, A.. (2021). Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes. in Biochemistry-Moscow
Maik Nauka/Interperiodica/Springer, New York., 86(11), 1434-1445.
https://doi.org/10.1134/S0006297921110079

Balint V, Stanisavljević Ninković D, Anastasov N, Lazić S, Kovačević Grujičić N, Stevanović M, Lazić A. Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes. in Biochemistry-Moscow. 2021;86(11):1434-1445.
doi:10.1134/S0006297921110079 .

Balint, Vanda, Stanisavljević Ninković, Danijela, Anastasov, Nataša, Lazić, Stefan, Kovačević Grujičić, Nataša, Stevanović, Milena, Lazić, Andrijana, "Inhibition of miR-21 Promotes Cellular Senescence in NT2-Derived Astrocytes" in Biochemistry-Moscow, 86, no. 11 (2021):1434-1445,
https://doi.org/10.1134/S0006297921110079 . .