TY  - CONF
AU  - Marjanović, Jelena
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1550
PB  - Springernature, London
C3  - European Journal of Human Genetics
T1  - SOX3 function in glioblastoma cells
EP  - 429
IS  - SUPPL 1
SP  - 428
VL  - 30
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1550
ER  - 

@conference{
author = "Marjanović, Jelena and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Garros-Regulez, Laura and Sampron, Nicolas and Matheu, Ander and Stevanović, Milena",
year = "2022",
publisher = "Springernature, London",
journal = "European Journal of Human Genetics",
title = "SOX3 function in glioblastoma cells",
pages = "429-428",
number = "SUPPL 1",
volume = "30",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1550"
}

Marjanović, J., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Garros-Regulez, L., Sampron, N., Matheu, A.,& Stevanović, M.. (2022). SOX3 function in glioblastoma cells. in European Journal of Human Genetics
Springernature, London., 30(SUPPL 1), 428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550

Marjanović J, Drakulić D, Garcia I, Vuković V, Aldaz P, Garros-Regulez L, Sampron N, Matheu A, Stevanović M. SOX3 function in glioblastoma cells. in European Journal of Human Genetics. 2022;30(SUPPL 1):428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

Marjanović, Jelena, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Garros-Regulez, Laura, Sampron, Nicolas, Matheu, Ander, Stevanović, Milena, "SOX3 function in glioblastoma cells" in European Journal of Human Genetics, 30, no. SUPPL 1 (2022):428-429,
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1629
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1206
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - JOUR
AU  - Vicentić, Jelena Marjanovic
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Puskas, Nela
AU  - Nikolić, Igor
AU  - Tasić, Goran
AU  - Raicević, Savo
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1285
AB  - PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.
PB  - Springer, Dordrecht
T2  - Cellular Oncology
T1  - SOX3 can promote the malignant behavior of glioblastoma cells
EP  - 54
IS  - 1
SP  - 41
VL  - 42
DO  - 10.1007/s13402-018-0405-5
ER  - 

@article{
author = "Vicentić, Jelena Marjanovic and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Puskas, Nela and Nikolić, Igor and Tasić, Goran and Raicević, Savo and Garros-Regulez, Laura and Sampron, Nicolas and Atkinson, Michael J. and Anastasov, Nataša and Matheu, Ander and Stevanović, Milena",
year = "2019",
abstract = "PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.",
publisher = "Springer, Dordrecht",
journal = "Cellular Oncology",
title = "SOX3 can promote the malignant behavior of glioblastoma cells",
pages = "54-41",
number = "1",
volume = "42",
doi = "10.1007/s13402-018-0405-5"
}

Vicentić, J. M., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Puskas, N., Nikolić, I., Tasić, G., Raicević, S., Garros-Regulez, L., Sampron, N., Atkinson, M. J., Anastasov, N., Matheu, A.,& Stevanović, M.. (2019). SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology
Springer, Dordrecht., 42(1), 41-54.
https://doi.org/10.1007/s13402-018-0405-5

Vicentić JM, Drakulić D, Garcia I, Vuković V, Aldaz P, Puskas N, Nikolić I, Tasić G, Raicević S, Garros-Regulez L, Sampron N, Atkinson MJ, Anastasov N, Matheu A, Stevanović M. SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology. 2019;42(1):41-54.
doi:10.1007/s13402-018-0405-5 .

Vicentić, Jelena Marjanovic, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Puskas, Nela, Nikolić, Igor, Tasić, Goran, Raicević, Savo, Garros-Regulez, Laura, Sampron, Nicolas, Atkinson, Michael J., Anastasov, Nataša, Matheu, Ander, Stevanović, Milena, "SOX3 can promote the malignant behavior of glioblastoma cells" in Cellular Oncology, 42, no. 1 (2019):41-54,
https://doi.org/10.1007/s13402-018-0405-5 . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Vicentić, Jelena Marjanovic
AU  - Schwirtlich, Marija
AU  - Tosić, Jelena
AU  - Krstić, Aleksandar
AU  - Lazić, Andrijana
AU  - Stevanović, Milena
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/889
AB  - The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis.
PB  - Acad Brasileira De Ciencias, Rio Janeiro
T2  - Anais da Academia Brasileira de Ciencias
T1  - The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1
EP  - 405
IS  - 1
SP  - 389
VL  - 87
DO  - 10.1590/0001-3765201520140352
ER  - 

@article{
author = "Drakulić, Danijela and Vicentić, Jelena Marjanovic and Schwirtlich, Marija and Tosić, Jelena and Krstić, Aleksandar and Lazić, Andrijana and Stevanović, Milena",
year = "2015",
abstract = "The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis.",
publisher = "Acad Brasileira De Ciencias, Rio Janeiro",
journal = "Anais da Academia Brasileira de Ciencias",
title = "The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1",
pages = "405-389",
number = "1",
volume = "87",
doi = "10.1590/0001-3765201520140352"
}

Drakulić, D., Vicentić, J. M., Schwirtlich, M., Tosić, J., Krstić, A., Lazić, A.,& Stevanović, M.. (2015). The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1. in Anais da Academia Brasileira de Ciencias
Acad Brasileira De Ciencias, Rio Janeiro., 87(1), 389-405.
https://doi.org/10.1590/0001-3765201520140352

Drakulić D, Vicentić JM, Schwirtlich M, Tosić J, Krstić A, Lazić A, Stevanović M. The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1. in Anais da Academia Brasileira de Ciencias. 2015;87(1):389-405.
doi:10.1590/0001-3765201520140352 .

Drakulić, Danijela, Vicentić, Jelena Marjanovic, Schwirtlich, Marija, Tosić, Jelena, Krstić, Aleksandar, Lazić, Andrijana, Stevanović, Milena, "The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1" in Anais da Academia Brasileira de Ciencias, 87, no. 1 (2015):389-405,
https://doi.org/10.1590/0001-3765201520140352 . .