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Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation

Goff, Miriam; Nikodinović-Runić, Jasmina; O'Connor, Kevin

(Oxford Univ Press, Oxford, 2009)

TY  - JOUR
AU  - Goff, Miriam
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin 
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/364
AB  - A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L-1.
PB  - Oxford Univ Press, Oxford
T2  - FEMS Microbiology Letters
T1  - Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation
EP  - 305
IS  - 2
SP  - 297
VL  - 292
DO  - 10.1111/j.1574-6968.2009.01504.x
ER  - 
@article{
author = "Goff, Miriam and Nikodinović-Runić, Jasmina and O'Connor, Kevin ",
year = "2009",
abstract = "A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L-1.",
publisher = "Oxford Univ Press, Oxford",
journal = "FEMS Microbiology Letters",
title = "Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation",
pages = "305-297",
number = "2",
volume = "292",
doi = "10.1111/j.1574-6968.2009.01504.x"
}
Goff, M., Nikodinović-Runić, J.,& O'Connor, K.. (2009). Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation. in FEMS Microbiology Letters
Oxford Univ Press, Oxford., 292(2), 297-305.
https://doi.org/10.1111/j.1574-6968.2009.01504.x
Goff M, Nikodinović-Runić J, O'Connor K. Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation. in FEMS Microbiology Letters. 2009;292(2):297-305.
doi:10.1111/j.1574-6968.2009.01504.x .
Goff, Miriam, Nikodinović-Runić, Jasmina, O'Connor, Kevin , "Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation" in FEMS Microbiology Letters, 292, no. 2 (2009):297-305,
https://doi.org/10.1111/j.1574-6968.2009.01504.x . .
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