TY  - JOUR
AU  - Hume, Aisling R.
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin 
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/377
AB  - A fatty acyl coenzyme A synthetase (FadD) from Pseudomonas putida CA-3 is capable of activating a wide range of phenylalkanoic and alkanoic acids. It exhibits the highest rates of reaction and catalytic efficiency with long-chain aromatic and aliphatic substrates. FadD exhibits higher k(cat) and Km values for aromatic substrates than for the aliphatic equivalents (e. g., 15-phenylpentadecanoic acid versus pentadecanoic acid). FadD is inhibited noncompetitively by both acrylic acid and 2-bromooctanoic acid. The deletion of the fadD gene from P. putida CA-3 resulted in no detectable growth or polyhydroxyalkanoate (PHA) accumulation with 10-phenyldecanoic acid, decanoic acid, and longer-chain substrates. The results suggest that FadD is solely responsible for the activation of long-chain phenylalkanoic and alkanoic acids. While the CA-3 Delta fadD mutant could grow on medium-chain substrates, a decrease in growth yield and PHA accumulation was observed. The PHA accumulated by CA-3 Delta fadD contained a greater proportion of short-chain monomers than did wild-type PHA. Growth of CA-3 Delta fadD was unaffected, but PHA accumulation decreased modestly with shorter-chain substrates. The complemented mutant regained 70% to 90% of the growth and PHA-accumulating ability of the wild-type strain depending on the substrate. The expression of an extra copy of fadD in P. putida CA-3 resulted in increased levels of PHA accumulation (up to 1.6-fold) and an increase in the incorporation of longer-monomer units into the PHA polymer.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - FadD from Pseudomonas putida CA-3 Is a True Long-Chain Fatty Acyl Coenzyme A Synthetase That Activates Phenylalkanoic and Alkanoic Acids
EP  - 7565
IS  - 24
SP  - 7554
VL  - 191
DO  - 10.1128/JB.01016-09
ER  - 

@article{
author = "Hume, Aisling R. and Nikodinović-Runić, Jasmina and O'Connor, Kevin ",
year = "2009",
abstract = "A fatty acyl coenzyme A synthetase (FadD) from Pseudomonas putida CA-3 is capable of activating a wide range of phenylalkanoic and alkanoic acids. It exhibits the highest rates of reaction and catalytic efficiency with long-chain aromatic and aliphatic substrates. FadD exhibits higher k(cat) and Km values for aromatic substrates than for the aliphatic equivalents (e. g., 15-phenylpentadecanoic acid versus pentadecanoic acid). FadD is inhibited noncompetitively by both acrylic acid and 2-bromooctanoic acid. The deletion of the fadD gene from P. putida CA-3 resulted in no detectable growth or polyhydroxyalkanoate (PHA) accumulation with 10-phenyldecanoic acid, decanoic acid, and longer-chain substrates. The results suggest that FadD is solely responsible for the activation of long-chain phenylalkanoic and alkanoic acids. While the CA-3 Delta fadD mutant could grow on medium-chain substrates, a decrease in growth yield and PHA accumulation was observed. The PHA accumulated by CA-3 Delta fadD contained a greater proportion of short-chain monomers than did wild-type PHA. Growth of CA-3 Delta fadD was unaffected, but PHA accumulation decreased modestly with shorter-chain substrates. The complemented mutant regained 70% to 90% of the growth and PHA-accumulating ability of the wild-type strain depending on the substrate. The expression of an extra copy of fadD in P. putida CA-3 resulted in increased levels of PHA accumulation (up to 1.6-fold) and an increase in the incorporation of longer-monomer units into the PHA polymer.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "FadD from Pseudomonas putida CA-3 Is a True Long-Chain Fatty Acyl Coenzyme A Synthetase That Activates Phenylalkanoic and Alkanoic Acids",
pages = "7565-7554",
number = "24",
volume = "191",
doi = "10.1128/JB.01016-09"
}

Hume, A. R., Nikodinović-Runić, J.,& O'Connor, K.. (2009). FadD from Pseudomonas putida CA-3 Is a True Long-Chain Fatty Acyl Coenzyme A Synthetase That Activates Phenylalkanoic and Alkanoic Acids. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 191(24), 7554-7565.
https://doi.org/10.1128/JB.01016-09

Hume AR, Nikodinović-Runić J, O'Connor K. FadD from Pseudomonas putida CA-3 Is a True Long-Chain Fatty Acyl Coenzyme A Synthetase That Activates Phenylalkanoic and Alkanoic Acids. in Journal of Bacteriology. 2009;191(24):7554-7565.
doi:10.1128/JB.01016-09 .

Hume, Aisling R., Nikodinović-Runić, Jasmina, O'Connor, Kevin , "FadD from Pseudomonas putida CA-3 Is a True Long-Chain Fatty Acyl Coenzyme A Synthetase That Activates Phenylalkanoic and Alkanoic Acids" in Journal of Bacteriology, 191, no. 24 (2009):7554-7565,
https://doi.org/10.1128/JB.01016-09 . .