TY  - JOUR
AU  - Simonović, J.
AU  - Toljić, B.
AU  - Lazarević, M.
AU  - Marković, M.M.
AU  - Perić, M.
AU  - Vujin, J.
AU  - Panajotović, R.
AU  - Milašin, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1545
AB  - Background: Dental stem cells, which originate from the neural crest, due to their easy accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry, multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film; (4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal, atomic force and scanning electron microscopy), followed by neural marker gene expression analysis using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’ expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted in neuron-like cellular phenotype observed under different microscopes. This was corroborated by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2, MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly enhanced by graphene film.
PB  - MDPI
T2  - Nanomaterials
T1  - The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla
IS  - 18
VL  - 12
DO  - 10.3390/nano12183116
ER  - 

@article{
author = "Simonović, J. and Toljić, B. and Lazarević, M. and Marković, M.M. and Perić, M. and Vujin, J. and Panajotović, R. and Milašin, Jelena",
year = "2022",
abstract = "Background: Dental stem cells, which originate from the neural crest, due to their easy accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry, multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film; (4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal, atomic force and scanning electron microscopy), followed by neural marker gene expression analysis using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’ expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted in neuron-like cellular phenotype observed under different microscopes. This was corroborated by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2, MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly enhanced by graphene film.",
publisher = "MDPI",
journal = "Nanomaterials",
title = "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla",
number = "18",
volume = "12",
doi = "10.3390/nano12183116"
}

Simonović, J., Toljić, B., Lazarević, M., Marković, M.M., Perić, M., Vujin, J., Panajotović, R.,& Milašin, J.. (2022). The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials
MDPI., 12(18).
https://doi.org/10.3390/nano12183116

Simonović J, Toljić B, Lazarević M, Marković M, Perić M, Vujin J, Panajotović R, Milašin J. The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials. 2022;12(18).
doi:10.3390/nano12183116 .

Simonović, J., Toljić, B., Lazarević, M., Marković, M.M., Perić, M., Vujin, J., Panajotović, R., Milašin, Jelena, "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla" in Nanomaterials, 12, no. 18 (2022),
https://doi.org/10.3390/nano12183116 . .

TY  - JOUR
AU  - Radunović, Milena
AU  - Pavić, Aleksandar
AU  - Ivanović, Vera
AU  - Milivojević, Marija
AU  - Radović, Igor
AU  - Di Carlo, Roberta
AU  - Pilato, Serena
AU  - Fontana, Antonella
AU  - Piattelli, Adriano
AU  - Petrović, Sanja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1511
AB  - The aims of the study were: 1) to evaluate the effect on biofilm formation of barrier membranes and titanium surfaces coated with graphene-oxide (GO); 2) to analyze the connection between the superficial topography of the tested materials and the amount of bacterial accumulation on them and 3) to analyze the biocompatibility of GO functionalized discs using the zebrafish model. Methods: Single species bacterial biofilms (Streptococcus oralis, Veilonella parvula, Fusobacterium nucleatum, Porphyomonas gingivalis) were grown on GO-free membranes, membranes coated with 2 and 10 mu g/ml of GO, GO-free and GO-coated titanium discs. The biofilms were analyzed by determining the CFU count and by Scanning Electron Microscopy (SEM) and the materials' topography by Atomic Force Microscopy (AFM). Zebrafish model was used to determine the materials' toxicity and inflammatory effects. Results: AFM showed similar roughness of control and GO-coated materials. CFU counts on GO-coated discs were significantly lower than on control discs for all species. CFU counts of S. oralis, V. parvula and P. gingivalis were lower on biofilms grown on both types of GOcoated membranes than on GO-free membrane. SEM analysis showed different formation of single species biofilm of S. oralis on control and GO-coated materials. GO-functionalized titanium discs do not induce toxic or inflammatory effects. Significance: Titanium implant surfaces functionalized with GO have shown to be biocompatible and less susceptible to biofilm formation. These results encourage further in vivo investigation of the tested materials on infection prevention, specifically in prevention and reduction of peri-implant mucositis and periimplantitis incidence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Dental Materials
T1  - Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes
EP  - 1127
IS  - 7
SP  - 1117
VL  - 38
DO  - 10.1016/j.dental.2022.04.024
ER  - 

@article{
author = "Radunović, Milena and Pavić, Aleksandar and Ivanović, Vera and Milivojević, Marija and Radović, Igor and Di Carlo, Roberta and Pilato, Serena and Fontana, Antonella and Piattelli, Adriano and Petrović, Sanja",
year = "2022",
abstract = "The aims of the study were: 1) to evaluate the effect on biofilm formation of barrier membranes and titanium surfaces coated with graphene-oxide (GO); 2) to analyze the connection between the superficial topography of the tested materials and the amount of bacterial accumulation on them and 3) to analyze the biocompatibility of GO functionalized discs using the zebrafish model. Methods: Single species bacterial biofilms (Streptococcus oralis, Veilonella parvula, Fusobacterium nucleatum, Porphyomonas gingivalis) were grown on GO-free membranes, membranes coated with 2 and 10 mu g/ml of GO, GO-free and GO-coated titanium discs. The biofilms were analyzed by determining the CFU count and by Scanning Electron Microscopy (SEM) and the materials' topography by Atomic Force Microscopy (AFM). Zebrafish model was used to determine the materials' toxicity and inflammatory effects. Results: AFM showed similar roughness of control and GO-coated materials. CFU counts on GO-coated discs were significantly lower than on control discs for all species. CFU counts of S. oralis, V. parvula and P. gingivalis were lower on biofilms grown on both types of GOcoated membranes than on GO-free membrane. SEM analysis showed different formation of single species biofilm of S. oralis on control and GO-coated materials. GO-functionalized titanium discs do not induce toxic or inflammatory effects. Significance: Titanium implant surfaces functionalized with GO have shown to be biocompatible and less susceptible to biofilm formation. These results encourage further in vivo investigation of the tested materials on infection prevention, specifically in prevention and reduction of peri-implant mucositis and periimplantitis incidence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Dental Materials",
title = "Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes",
pages = "1127-1117",
number = "7",
volume = "38",
doi = "10.1016/j.dental.2022.04.024"
}

Radunović, M., Pavić, A., Ivanović, V., Milivojević, M., Radović, I., Di Carlo, R., Pilato, S., Fontana, A., Piattelli, A.,& Petrović, S.. (2022). Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes. in Dental Materials
Elsevier Sci Ltd, Oxford., 38(7), 1117-1127.
https://doi.org/10.1016/j.dental.2022.04.024

Radunović M, Pavić A, Ivanović V, Milivojević M, Radović I, Di Carlo R, Pilato S, Fontana A, Piattelli A, Petrović S. Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes. in Dental Materials. 2022;38(7):1117-1127.
doi:10.1016/j.dental.2022.04.024 .

Radunović, Milena, Pavić, Aleksandar, Ivanović, Vera, Milivojević, Marija, Radović, Igor, Di Carlo, Roberta, Pilato, Serena, Fontana, Antonella, Piattelli, Adriano, Petrović, Sanja, "Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes" in Dental Materials, 38, no. 7 (2022):1117-1127,
https://doi.org/10.1016/j.dental.2022.04.024 . .