TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Dias, Maria Ines
AU  - Zengin, Gokhan
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Rajcević, Nemanja
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1574
AB  - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
PB  - EXCLI Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line
EP  - 399
SP  - 387
VL  - 21
DO  - 10.17179/excli2021-4487
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Ines and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajcević, Nemanja and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2022",
abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 mu g/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.",
publisher = "EXCLI Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line",
pages = "399-387",
volume = "21",
doi = "10.17179/excli2021-4487"
}

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajcević, N., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2022). Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal
EXCLI Journal Managing Office, Dortmund., 21, 387-399.
https://doi.org/10.17179/excli2021-4487

Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajcević N, Stevanović M, Ferreira ICFR, Soković M. Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. in EXCLI Journal. 2022;21:387-399.
doi:10.17179/excli2021-4487 .

Stojković, Dejan, Drakulić, Danijela, Dias, Maria Ines, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajcević, Nemanja, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Phlomis fruticosa l. Exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line" in EXCLI Journal, 21 (2022):387-399,
https://doi.org/10.17179/excli2021-4487 . .