TY  - JOUR
AU  - Gagić, Dragana
AU  - Ćirić, Milica
AU  - Wen, Wesley X.
AU  - Ng, Filomena
AU  - Rakonjac, Jasna
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/993
AB  - Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in the cell. A group of bacteriophage, called filamentous phage, have the ability to hijack bacterial protein secretion machinery in order to amplify and assemble via a secretion-like process. This ability has been harnessed in the use of filamentous phage of Escherichia coli in biotechnology applications, including screening large libraries of variants for binding to "bait" of interest, from tissues in vivo to pure proteins or even inorganic substrates. In this review we discuss the roles of secretome proteins in pathogenic and non-pathogenic bacteria and corresponding secretion pathways. We describe the basics of phage display technology and its variants applied to discovery of bacterial proteins that are implicated in colonization of host tissues and pathogenesis, as well as vaccine candidates through filamentous phage display library screening. Secretome selection aided by next-generation sequence analysis was successfully applied for selective display of the secretome at a microbial community scale, the latter revealing the richness of secretome functions of interest and surprising versatility in filamentous phage display of secretome proteins from large number of Gram-negative as well as Gram-positive bacteria and archaea.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display
VL  - 7
DO  - 10.3389/fmicb.2016.00429
ER  - 

@article{
author = "Gagić, Dragana and Ćirić, Milica and Wen, Wesley X. and Ng, Filomena and Rakonjac, Jasna",
year = "2016",
abstract = "Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in the cell. A group of bacteriophage, called filamentous phage, have the ability to hijack bacterial protein secretion machinery in order to amplify and assemble via a secretion-like process. This ability has been harnessed in the use of filamentous phage of Escherichia coli in biotechnology applications, including screening large libraries of variants for binding to "bait" of interest, from tissues in vivo to pure proteins or even inorganic substrates. In this review we discuss the roles of secretome proteins in pathogenic and non-pathogenic bacteria and corresponding secretion pathways. We describe the basics of phage display technology and its variants applied to discovery of bacterial proteins that are implicated in colonization of host tissues and pathogenesis, as well as vaccine candidates through filamentous phage display library screening. Secretome selection aided by next-generation sequence analysis was successfully applied for selective display of the secretome at a microbial community scale, the latter revealing the richness of secretome functions of interest and surprising versatility in filamentous phage display of secretome proteins from large number of Gram-negative as well as Gram-positive bacteria and archaea.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display",
volume = "7",
doi = "10.3389/fmicb.2016.00429"
}

Gagić, D., Ćirić, M., Wen, W. X., Ng, F.,& Rakonjac, J.. (2016). Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.00429

Gagić D, Ćirić M, Wen WX, Ng F, Rakonjac J. Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.00429 .

Gagić, Dragana, Ćirić, Milica, Wen, Wesley X., Ng, Filomena, Rakonjac, Jasna, "Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.00429 . .