TY  - JOUR
AU  - Tomić, Sergej
AU  - Đokić, Jelena
AU  - Vasilijić, Sasa
AU  - Vucević, Dragana
AU  - Todorović, Vera
AU  - Supić, Gordana
AU  - Čolić, Miodrag
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/529
AB  - Adult mesenchymal stem cells (MSCs) have recently become a potent tool in regenerative medicine. Due to certain shortcomings of obtaining bone marrow MSCs, alternate sources of MSCs have been sought. In this work, we studied MSCs from dental pulp (DP-MSCs) and dental follicle (DF-MSCs), isolated from the same tooth/donor, to define differences in their phenotypic properties, differentiation potential, and immunomodulatory activities. Both cell types showed colony-forming ability and expressed typical MSCs markers, but differed in the levels of their expression. DF-MSCs proliferated faster, contained cells larger in diameter, exhibited a higher potential to form adipocytes and a lower potential to form chondrocytes and osteoblasts, compared with DP-MSCs. In contrast to DF-MSCs, DP-MSCs produced the transforming growth factor (TGF)-beta and suppressed proliferation of peripheral blood mononuclear cells, which could be neutralized with anti-TGF-beta antibody. The treatment with toll-like receptor 3 (TLR3) agonist augmented the suppressive potential of both cell types and potentiated TGF-beta and interleukin-6 secretions by these cells. TLR4 agonist augmented the suppressive potential of DF-MSCs and increased TGF-beta production, but abrogated the immunosuppressive activity of DP-MSCs by inhibiting TGF-beta production and the expression of indolamine-2,3-dioxygenase-1. Some of these effects correlated with the higher expression of TLR3 and TLR4 by DP-MSCs compared with DF-MSCs. When transplanted in imunocompetent xenogenic host, both cell types induced formation of granulomatous tissue. In conclusion, our results suggest that dental MSCs are functionally different and each of these functions should be further explored in vivo before their specific biomedical applications.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Stem Cells and Development
T1  - Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists
EP  - 708
IS  - 4
SP  - 695
VL  - 20
DO  - 10.1089/scd.2010.0145
ER  - 

@article{
author = "Tomić, Sergej and Đokić, Jelena and Vasilijić, Sasa and Vucević, Dragana and Todorović, Vera and Supić, Gordana and Čolić, Miodrag",
year = "2011",
abstract = "Adult mesenchymal stem cells (MSCs) have recently become a potent tool in regenerative medicine. Due to certain shortcomings of obtaining bone marrow MSCs, alternate sources of MSCs have been sought. In this work, we studied MSCs from dental pulp (DP-MSCs) and dental follicle (DF-MSCs), isolated from the same tooth/donor, to define differences in their phenotypic properties, differentiation potential, and immunomodulatory activities. Both cell types showed colony-forming ability and expressed typical MSCs markers, but differed in the levels of their expression. DF-MSCs proliferated faster, contained cells larger in diameter, exhibited a higher potential to form adipocytes and a lower potential to form chondrocytes and osteoblasts, compared with DP-MSCs. In contrast to DF-MSCs, DP-MSCs produced the transforming growth factor (TGF)-beta and suppressed proliferation of peripheral blood mononuclear cells, which could be neutralized with anti-TGF-beta antibody. The treatment with toll-like receptor 3 (TLR3) agonist augmented the suppressive potential of both cell types and potentiated TGF-beta and interleukin-6 secretions by these cells. TLR4 agonist augmented the suppressive potential of DF-MSCs and increased TGF-beta production, but abrogated the immunosuppressive activity of DP-MSCs by inhibiting TGF-beta production and the expression of indolamine-2,3-dioxygenase-1. Some of these effects correlated with the higher expression of TLR3 and TLR4 by DP-MSCs compared with DF-MSCs. When transplanted in imunocompetent xenogenic host, both cell types induced formation of granulomatous tissue. In conclusion, our results suggest that dental MSCs are functionally different and each of these functions should be further explored in vivo before their specific biomedical applications.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Stem Cells and Development",
title = "Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists",
pages = "708-695",
number = "4",
volume = "20",
doi = "10.1089/scd.2010.0145"
}

Tomić, S., Đokić, J., Vasilijić, S., Vucević, D., Todorović, V., Supić, G.,& Čolić, M.. (2011). Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists. in Stem Cells and Development
Mary Ann Liebert, Inc, New Rochelle., 20(4), 695-708.
https://doi.org/10.1089/scd.2010.0145

Tomić S, Đokić J, Vasilijić S, Vucević D, Todorović V, Supić G, Čolić M. Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists. in Stem Cells and Development. 2011;20(4):695-708.
doi:10.1089/scd.2010.0145 .

Tomić, Sergej, Đokić, Jelena, Vasilijić, Sasa, Vucević, Dragana, Todorović, Vera, Supić, Gordana, Čolić, Miodrag, "Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists" in Stem Cells and Development, 20, no. 4 (2011):695-708,
https://doi.org/10.1089/scd.2010.0145 . .