TY  - JOUR
AU  - Makryniotis, Konstantinos
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2341
AB  - The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.
T2  - Biotechnology Journal
T2  - Biotechnology Journal
T1  - Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
IS  - n/a
SP  - 2400053
VL  - n/a
DO  - 10.1002/biot.202400053
ER  - 

@article{
author = "Makryniotis, Konstantinos and Nikolaivits, Efstratios and Taxeidis, George and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
abstract = "The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.",
journal = "Biotechnology Journal, Biotechnology Journal",
title = "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases",
number = "n/a",
pages = "2400053",
volume = "n/a",
doi = "10.1002/biot.202400053"
}

Makryniotis, K., Nikolaivits, E., Taxeidis, G., Nikodinović-Runić, J.,& Topakas, E..Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal, n/a(n/a), 2400053.
https://doi.org/10.1002/biot.202400053

Makryniotis K, Nikolaivits E, Taxeidis G, Nikodinović-Runić J, Topakas E. Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal.n/a(n/a):2400053.
doi:10.1002/biot.202400053 .

Makryniotis, Konstantinos, Nikolaivits, Efstratios, Taxeidis, George, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases" in Biotechnology Journal, n/a, no. n/a:2400053,
https://doi.org/10.1002/biot.202400053 . .

TY  - JOUR
AU  - Makryniotis, Konstantinos
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2345
AB  - The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.
T2  - Biotechnology Journal
T1  - Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
IS  - n/a
SP  - 2400053
VL  - n/a
DO  - 10.1002/biot.202400053
ER  - 

@article{
author = "Makryniotis, Konstantinos and Nikolaivits, Efstratios and Taxeidis, George and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
abstract = "The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.",
journal = "Biotechnology Journal",
title = "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases",
number = "n/a",
pages = "2400053",
volume = "n/a",
doi = "10.1002/biot.202400053"
}

Makryniotis, K., Nikolaivits, E., Taxeidis, G., Nikodinović-Runić, J.,& Topakas, E..Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal, n/a(n/a), 2400053.
https://doi.org/10.1002/biot.202400053

Makryniotis K, Nikolaivits E, Taxeidis G, Nikodinović-Runić J, Topakas E. Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal.n/a(n/a):2400053.
doi:10.1002/biot.202400053 .

Makryniotis, Konstantinos, Nikolaivits, Efstratios, Taxeidis, George, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases" in Biotechnology Journal, n/a, no. n/a:2400053,
https://doi.org/10.1002/biot.202400053 . .

TY  - JOUR
AU  - Milovanovic, V.
AU  - Topic, A.
AU  - Milinkovic, N.
AU  - Lazic, Z.
AU  - Ivosevic, A.
AU  - Radojkovic, D.
AU  - Divac Rankov, Aleksandra
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1647
AB  - ObjectiveChronic obstructive pulmonary disease (COPD) is multi–factorial disorder which results from environmental influences and genetic factors. We aimed to investigate whether methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with COPD development and severity in Serbian adult population.MethodsThe study included 155 patients with COPD and 134 healthy volunteers. Genotyping was determined performing home-made polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxidized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxidized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).ResultsFrequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in genotype or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was significantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-Elastase) was significantly lower in COPD patients than in the control group. In COPD group, increased level of OxyA1AT was present in G allele carriers who were smokers relative to G allele carriers who were not smokers. In the smoker group of patients with severe and very severe COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.ConclusionThese findings suggest that MSRA rs10903323 gene polymorphism is probably not a risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized A1AT in COPD-smokers, and in severe form of COPD.
PB  - Elsevier
T2  - Pulmonology
T2  - Pulmonology
T1  - Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients
EP  - 129
IS  - 2
SP  - 122
VL  - 30
DO  - 10.1016/j.pulmoe.2021.09.003
ER  - 

@article{
author = "Milovanovic, V. and Topic, A. and Milinkovic, N. and Lazic, Z. and Ivosevic, A. and Radojkovic, D. and Divac Rankov, Aleksandra",
year = "2024",
abstract = "ObjectiveChronic obstructive pulmonary disease (COPD) is multi–factorial disorder which results from environmental influences and genetic factors. We aimed to investigate whether methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with COPD development and severity in Serbian adult population.MethodsThe study included 155 patients with COPD and 134 healthy volunteers. Genotyping was determined performing home-made polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxidized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxidized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).ResultsFrequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in genotype or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was significantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-Elastase) was significantly lower in COPD patients than in the control group. In COPD group, increased level of OxyA1AT was present in G allele carriers who were smokers relative to G allele carriers who were not smokers. In the smoker group of patients with severe and very severe COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.ConclusionThese findings suggest that MSRA rs10903323 gene polymorphism is probably not a risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized A1AT in COPD-smokers, and in severe form of COPD.",
publisher = "Elsevier",
journal = "Pulmonology, Pulmonology",
title = "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients",
pages = "129-122",
number = "2",
volume = "30",
doi = "10.1016/j.pulmoe.2021.09.003"
}

Milovanovic, V., Topic, A., Milinkovic, N., Lazic, Z., Ivosevic, A., Radojkovic, D.,& Divac Rankov, A.. (2024). Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology
Elsevier., 30(2), 122-129.
https://doi.org/10.1016/j.pulmoe.2021.09.003

Milovanovic V, Topic A, Milinkovic N, Lazic Z, Ivosevic A, Radojkovic D, Divac Rankov A. Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology. 2024;30(2):122-129.
doi:10.1016/j.pulmoe.2021.09.003 .

Milovanovic, V., Topic, A., Milinkovic, N., Lazic, Z., Ivosevic, A., Radojkovic, D., Divac Rankov, Aleksandra, "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients" in Pulmonology, 30, no. 2 (2024):122-129,
https://doi.org/10.1016/j.pulmoe.2021.09.003 . .

TY  - CONF
AU  - Tsibulskaya, Darya
AU  - Blagojević, Veljko
AU  - Terzić-Vidojević, Amarela
AU  - Lukić, Ivana
AU  - Vasić, Marko
AU  - Dragačević, Luka
AU  - Kojić, Milan
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2373
AB  - Autoaggregation, the ability to self-aggregate,
is widespread among both Gram-positive and
Gram-negative bacteria. The functional role
of aggregation is not fully understood, but it
is believed to be involved in the adaptation of
bacteria to environmental conditions (PMID:
31294207). One interesting class of compounds
responsible for the aggregation of lactic
acid bacteria is aggregation factors—surface
high-molecular-weight proteins rich in threonine
and lysine (PMID: 30027759). Recently,
our research group discovered a new strain of
Streptococcus thermophilus in the mountainous
regions of Serbia, exhibiting an aggregation
phenotype. Aggregation phenotype was confirmed
visually and using microscopy. Complete
genome of Agg+ strain was sequenced using
NGS and a gene encoding a potential aggregation
factor, which was named aggS was identified.
The predicted threonine (12.5%) and lysine
(10.5%) rich protein contains 2367 amino acids,
with an average molecular weight of 255986.63
Da. AggS also contains two cysteine residues,whereas previously well-described aggregation
factors of this type did not contain any cysteine
residues. The predicted protein includes an
N-terminal YSIRK-like signal sequence and an
LPXTG cell wall anchor domain. It has 6 Mucin
binding domain repeats alternating with 6 Mub
B2-like domain repeats. Additionally, we found a
region resembling an ice-binding domain. Given
that these bacteria endure prolonged periods of
low temperatures, it can be speculated that this
surface membrane protein also helps the bacteria
withstand freezing. The fact that the alignment
using BLASTp revealed AggS to be most
closely related to an uncharacterised protein
from the genome of Lactococcus garvieae, along
with the discovery of a transposase gene sequence
upstream of the gene, suggests that the
aggregation factor was likely acquired through
horizontal gene transfer. We plan to clone it into
a shuttle vector and investigate the aggregation
phenotype using a heterologous expression system
in Lactococcus lactis, as well as explore its
other functions.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - DESCRIPTION OF A NEW POTENTIAL AGGREGATION FACTOR FROM THE STREPTOCOCCUS THERMOPHILUS GENOME
EP  - 110
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2373
ER  - 

@conference{
author = "Tsibulskaya, Darya and Blagojević, Veljko and Terzić-Vidojević, Amarela and Lukić, Ivana and Vasić, Marko and Dragačević, Luka and Kojić, Milan",
year = "2024",
abstract = "Autoaggregation, the ability to self-aggregate,
is widespread among both Gram-positive and
Gram-negative bacteria. The functional role
of aggregation is not fully understood, but it
is believed to be involved in the adaptation of
bacteria to environmental conditions (PMID:
31294207). One interesting class of compounds
responsible for the aggregation of lactic
acid bacteria is aggregation factors—surface
high-molecular-weight proteins rich in threonine
and lysine (PMID: 30027759). Recently,
our research group discovered a new strain of
Streptococcus thermophilus in the mountainous
regions of Serbia, exhibiting an aggregation
phenotype. Aggregation phenotype was confirmed
visually and using microscopy. Complete
genome of Agg+ strain was sequenced using
NGS and a gene encoding a potential aggregation
factor, which was named aggS was identified.
The predicted threonine (12.5%) and lysine
(10.5%) rich protein contains 2367 amino acids,
with an average molecular weight of 255986.63
Da. AggS also contains two cysteine residues,whereas previously well-described aggregation
factors of this type did not contain any cysteine
residues. The predicted protein includes an
N-terminal YSIRK-like signal sequence and an
LPXTG cell wall anchor domain. It has 6 Mucin
binding domain repeats alternating with 6 Mub
B2-like domain repeats. Additionally, we found a
region resembling an ice-binding domain. Given
that these bacteria endure prolonged periods of
low temperatures, it can be speculated that this
surface membrane protein also helps the bacteria
withstand freezing. The fact that the alignment
using BLASTp revealed AggS to be most
closely related to an uncharacterised protein
from the genome of Lactococcus garvieae, along
with the discovery of a transposase gene sequence
upstream of the gene, suggests that the
aggregation factor was likely acquired through
horizontal gene transfer. We plan to clone it into
a shuttle vector and investigate the aggregation
phenotype using a heterologous expression system
in Lactococcus lactis, as well as explore its
other functions.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "DESCRIPTION OF A NEW POTENTIAL AGGREGATION FACTOR FROM THE STREPTOCOCCUS THERMOPHILUS GENOME",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2373"
}

Tsibulskaya, D., Blagojević, V., Terzić-Vidojević, A., Lukić, I., Vasić, M., Dragačević, L.,& Kojić, M.. (2024). DESCRIPTION OF A NEW POTENTIAL AGGREGATION FACTOR FROM THE STREPTOCOCCUS THERMOPHILUS GENOME. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 110-110.
https://hdl.handle.net/21.15107/rcub_imagine_2373

Tsibulskaya D, Blagojević V, Terzić-Vidojević A, Lukić I, Vasić M, Dragačević L, Kojić M. DESCRIPTION OF A NEW POTENTIAL AGGREGATION FACTOR FROM THE STREPTOCOCCUS THERMOPHILUS GENOME. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:110-110.
https://hdl.handle.net/21.15107/rcub_imagine_2373 .

Tsibulskaya, Darya, Blagojević, Veljko, Terzić-Vidojević, Amarela, Lukić, Ivana, Vasić, Marko, Dragačević, Luka, Kojić, Milan, "DESCRIPTION OF A NEW POTENTIAL AGGREGATION FACTOR FROM THE STREPTOCOCCUS THERMOPHILUS GENOME" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):110-110,
https://hdl.handle.net/21.15107/rcub_imagine_2373 .

TY  - CONF
AU  - Golić, Nataša
AU  - Terzić Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Dinić, Miroslav
AU  - Đokić, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Lukić, Jovanka
AU  - Živković, Milica
AU  - Nastasijević, Branislav
AU  - Soković, Svetlana
AU  - Brdarić, Emilija
AU  - Radojević, Dušan
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2372
AB  - There has been an epidemic of various non-communicable
degenerative and autoimmune diseases,
strongly associated with the modern
lifestyle. Among them, neurodegenerative and
psychiatric disorders represent a huge burden on
society. Recently, all these diseases have been associated
with the gut microbiota dysbiosis. Gut
microbiota-host interaction research has been
greatly improved due to development of molecular
high-throughput techniques based on
various ‘omics’ techniques coupled with bioinformatics
and data science developments. However,
the mechanisms of the host–microbiota crosstalk
are still poorly understood. The NextGenBiotics
project proposes an innovative integrative
multi-omics research strategy for deciphering
the mechanism behind the cross-talk among
microbiota and gut-brain-axis. The 118 novel
NGPs candidates belonging to Dorea sp., Blautia
sp., Bacteroides sp., Roseburia sp., Sellimonas
sp., Faecalicatena sp., Phascolarctobacterium faecium,
and Faecalimonas sp. were cultivated. The
25 NGPs with confirmed safe status and potential
probiotic potential were screened in C. elegans
model for their effects on behavioural and neuronal
activity. The most prominent candidates
with ability to upregulate expression of genes
involved in neurotransmiting are further tested
in EAE (an animal model for MS) and CUMS depression
model. The specific microbiota-derived
metabolites have been identified as potential
neuro- and psycho-biotics. The NextGenBiotics is
highly ambitious project, dedicated to pioneering
work in the field of multi-omics studies related
to the cultivation of novel anaerobic NGPs
and the studying of their effect on MGBA. This
concept enabled studying bidirectional communication
between gut microbiota and brain
on the functional level that will significantly
contribute to the growing body data related to
MGBA. The results obtained during NextGenBiotics
determined the genes/metabolites and the
associated mechanisms involved in health-promoting
effects of NGPs in MGBA beyond stateof-
the-art, broadening the scientific knowledge
and opening up the possible novel therapeutic
approaches in prevention and therapy of neurodegenerative
and psychiatric diseases.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS
EP  - 106
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2372
ER  - 

@conference{
author = "Golić, Nataša and Terzić Vidojević, Amarela and Tolinački, Maja and Dinić, Miroslav and Đokić, Jelena and Todorović Vukotić, Nevena and Lukić, Jovanka and Živković, Milica and Nastasijević, Branislav and Soković, Svetlana and Brdarić, Emilija and Radojević, Dušan",
year = "2024",
abstract = "There has been an epidemic of various non-communicable
degenerative and autoimmune diseases,
strongly associated with the modern
lifestyle. Among them, neurodegenerative and
psychiatric disorders represent a huge burden on
society. Recently, all these diseases have been associated
with the gut microbiota dysbiosis. Gut
microbiota-host interaction research has been
greatly improved due to development of molecular
high-throughput techniques based on
various ‘omics’ techniques coupled with bioinformatics
and data science developments. However,
the mechanisms of the host–microbiota crosstalk
are still poorly understood. The NextGenBiotics
project proposes an innovative integrative
multi-omics research strategy for deciphering
the mechanism behind the cross-talk among
microbiota and gut-brain-axis. The 118 novel
NGPs candidates belonging to Dorea sp., Blautia
sp., Bacteroides sp., Roseburia sp., Sellimonas
sp., Faecalicatena sp., Phascolarctobacterium faecium,
and Faecalimonas sp. were cultivated. The
25 NGPs with confirmed safe status and potential
probiotic potential were screened in C. elegans
model for their effects on behavioural and neuronal
activity. The most prominent candidates
with ability to upregulate expression of genes
involved in neurotransmiting are further tested
in EAE (an animal model for MS) and CUMS depression
model. The specific microbiota-derived
metabolites have been identified as potential
neuro- and psycho-biotics. The NextGenBiotics is
highly ambitious project, dedicated to pioneering
work in the field of multi-omics studies related
to the cultivation of novel anaerobic NGPs
and the studying of their effect on MGBA. This
concept enabled studying bidirectional communication
between gut microbiota and brain
on the functional level that will significantly
contribute to the growing body data related to
MGBA. The results obtained during NextGenBiotics
determined the genes/metabolites and the
associated mechanisms involved in health-promoting
effects of NGPs in MGBA beyond stateof-
the-art, broadening the scientific knowledge
and opening up the possible novel therapeutic
approaches in prevention and therapy of neurodegenerative
and psychiatric diseases.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS",
pages = "106-106",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2372"
}

Golić, N., Terzić Vidojević, A., Tolinački, M., Dinić, M., Đokić, J., Todorović Vukotić, N., Lukić, J., Živković, M., Nastasijević, B., Soković, S., Brdarić, E.,& Radojević, D.. (2024). THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 106-106.
https://hdl.handle.net/21.15107/rcub_imagine_2372

Golić N, Terzić Vidojević A, Tolinački M, Dinić M, Đokić J, Todorović Vukotić N, Lukić J, Živković M, Nastasijević B, Soković S, Brdarić E, Radojević D. THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:106-106.
https://hdl.handle.net/21.15107/rcub_imagine_2372 .

Golić, Nataša, Terzić Vidojević, Amarela, Tolinački, Maja, Dinić, Miroslav, Đokić, Jelena, Todorović Vukotić, Nevena, Lukić, Jovanka, Živković, Milica, Nastasijević, Branislav, Soković, Svetlana, Brdarić, Emilija, Radojević, Dušan, "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):106-106,
https://hdl.handle.net/21.15107/rcub_imagine_2372 .

TY  - CONF
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Plačkić, Nikola
AU  - Kljajević, Nemanja
AU  - Pavić, Aleksandar
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2375
AB  - Antimicrobial resistance (AMR) arises when
bacteria and other microbes stop responding
to medications. AMR is now recognized as one
of serious global health threats, repeatedly
appearing in the World Health Organization’s
(WHO) lists of urgent global health challenges,
including the 2024 list. It is taking a fatal toll
– nearly 5 million deaths globally per year are
associated with AMR, encompassing 1.27 million
directly attributed to AMR. The COVID-19
pandemic paved the way for aggravation of
bacterial AMR – primarily due to enhancement
in unspecific and unjustified prescription and
use of broad-spectrum antibiotics, resulting in
what is now recognized as „silent pandemic of
AMR“. Bacteriophages (phages) are natural and
specific predators of bacteria - viruses that can
infect, replicate inside and lyse arguably any
bacteria. Their therapeutic potential is being
hastily evaluated through different approaches:
in silico, in vitro, ex vivo and in vivo – in laboratory
animals as well as in human case and clinical
studies. Although the results are promising,bacteria rapidly develop resistance against
phages, which why the isolation and research
of new phages is needed. Our work is concentrated
on three bacterial species for which critical
priority by WHO has been declared – carbapenem-
resistant Acinetobacter baumannii,
Pseudomonas aeruginosa and Klebsiella pneumoniae.
Twenty distinct pathogenic strains of
A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa
were used as targets for bacteriophage
isolation, and total of 14, 22 and 8 potentially
distinct phages were collected, respectively. All
strains were nosocomial isolates obtained from
various tissues, including from terminally ill patients.
Six phages were characterized in detail.
In particular, phage vB_AbaM_ISTD was applied
against A. baumannii in zebrafish embryo
model of systemic infection, and demonstrated
powerful therapeutic potential, eradicating the
infection. Interestingly, its DNA was characterized
with highly modified thymidine (amassing
1228 Da), making it the largest non-canonical
deoxynucleoside reported so far.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE
EP  - 121
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2375
ER  - 

@conference{
author = "Vukotić, Goran and Obradović, Mina and Plačkić, Nikola and Kljajević, Nemanja and Pavić, Aleksandar and Kekić, Dušan and Gajić, Ina and Kojić, Milan and Stanisavljević, Nemanja",
year = "2024",
abstract = "Antimicrobial resistance (AMR) arises when
bacteria and other microbes stop responding
to medications. AMR is now recognized as one
of serious global health threats, repeatedly
appearing in the World Health Organization’s
(WHO) lists of urgent global health challenges,
including the 2024 list. It is taking a fatal toll
– nearly 5 million deaths globally per year are
associated with AMR, encompassing 1.27 million
directly attributed to AMR. The COVID-19
pandemic paved the way for aggravation of
bacterial AMR – primarily due to enhancement
in unspecific and unjustified prescription and
use of broad-spectrum antibiotics, resulting in
what is now recognized as „silent pandemic of
AMR“. Bacteriophages (phages) are natural and
specific predators of bacteria - viruses that can
infect, replicate inside and lyse arguably any
bacteria. Their therapeutic potential is being
hastily evaluated through different approaches:
in silico, in vitro, ex vivo and in vivo – in laboratory
animals as well as in human case and clinical
studies. Although the results are promising,bacteria rapidly develop resistance against
phages, which why the isolation and research
of new phages is needed. Our work is concentrated
on three bacterial species for which critical
priority by WHO has been declared – carbapenem-
resistant Acinetobacter baumannii,
Pseudomonas aeruginosa and Klebsiella pneumoniae.
Twenty distinct pathogenic strains of
A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa
were used as targets for bacteriophage
isolation, and total of 14, 22 and 8 potentially
distinct phages were collected, respectively. All
strains were nosocomial isolates obtained from
various tissues, including from terminally ill patients.
Six phages were characterized in detail.
In particular, phage vB_AbaM_ISTD was applied
against A. baumannii in zebrafish embryo
model of systemic infection, and demonstrated
powerful therapeutic potential, eradicating the
infection. Interestingly, its DNA was characterized
with highly modified thymidine (amassing
1228 Da), making it the largest non-canonical
deoxynucleoside reported so far.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2375"
}

Vukotić, G., Obradović, M., Plačkić, N., Kljajević, N., Pavić, A., Kekić, D., Gajić, I., Kojić, M.,& Stanisavljević, N.. (2024). BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2375

Vukotić G, Obradović M, Plačkić N, Kljajević N, Pavić A, Kekić D, Gajić I, Kojić M, Stanisavljević N. BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2375 .

Vukotić, Goran, Obradović, Mina, Plačkić, Nikola, Kljajević, Nemanja, Pavić, Aleksandar, Kekić, Dušan, Gajić, Ina, Kojić, Milan, Stanisavljević, Nemanja, "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):121-121,
https://hdl.handle.net/21.15107/rcub_imagine_2375 .

TY  - CONF
AU  - Janković, Vukašin
AU  - Pantelić, Brana
AU  - Jeremić, Sanja
AU  - Radetić, Maja
AU  - Marković, Darka
AU  - Kalogirou, Charalampia
AU  - Ilić-Tomić, Tatjana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2371
AB  - The increasing production and utilization of
synthetic polymers in the textile industry over
the past five decades has raised concerns about
the environmental impact of the industry. The
recalcitrant nature of synthetic fibers hampers
the biodegradation of these textiles in the environment
and leads to the accumulation of textile
waste. Effective solutions for recycling and proper
disposal of textile waste are lacking, however,
the use of microorganisms and enzymes has
emerged as a promising approach. The genus
Streptomyces has been well studied as a producer
of different hydrolytic enzymes, several of which
have found use in industrial settings as well. As
an integral part of the soil microbiome, Streptomyces
species have been shown to interact with
different textile materials in soil and may play a
role in the degradation of these materials. This
study aimed to examine the interaction of Streptomyces
sp. R1, isolated from the rhizosphere of
Cotinus coggygria, with polyamide/polyurethane
textile, and identify potential enzymes involved in the biodegradation of synthetic textiles. The
degradation of the textile was tested in liquid
cultures (minimal salt medium) and model compost,
bio-augmented with Streptomyces sp. R1
for 4 months. After the incubation, morphological,
and changes in the functional groups of the
textiles were analysed using scanning electron
microscopy (SEM) and Fourier transform infrared
spectroscopy (FTIR). The surface of the textile
showed noticeable cracks and fissures after
4 months of burial in the bioaugmented model
compost, alongside changes in the functional
groups of the polyamide/polyurethane textile,
which indicates biodegradation of the synthetic
fibers. Searching the genome of Streptomyces sp.
R1, several enzymes involved in the degradation
of synthetic polymers were identified, including
an esterase homologous to highly efficient plastic
degrading depolymerases. Overall, the results
presented here indicate Streptomyces sp. R1 has
the potential for synthetic textile degradation
and bioremediation.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2371
ER  - 

@conference{
author = "Janković, Vukašin and Pantelić, Brana and Jeremić, Sanja and Radetić, Maja and Marković, Darka and Kalogirou, Charalampia and Ilić-Tomić, Tatjana",
year = "2024",
abstract = "The increasing production and utilization of
synthetic polymers in the textile industry over
the past five decades has raised concerns about
the environmental impact of the industry. The
recalcitrant nature of synthetic fibers hampers
the biodegradation of these textiles in the environment
and leads to the accumulation of textile
waste. Effective solutions for recycling and proper
disposal of textile waste are lacking, however,
the use of microorganisms and enzymes has
emerged as a promising approach. The genus
Streptomyces has been well studied as a producer
of different hydrolytic enzymes, several of which
have found use in industrial settings as well. As
an integral part of the soil microbiome, Streptomyces
species have been shown to interact with
different textile materials in soil and may play a
role in the degradation of these materials. This
study aimed to examine the interaction of Streptomyces
sp. R1, isolated from the rhizosphere of
Cotinus coggygria, with polyamide/polyurethane
textile, and identify potential enzymes involved in the biodegradation of synthetic textiles. The
degradation of the textile was tested in liquid
cultures (minimal salt medium) and model compost,
bio-augmented with Streptomyces sp. R1
for 4 months. After the incubation, morphological,
and changes in the functional groups of the
textiles were analysed using scanning electron
microscopy (SEM) and Fourier transform infrared
spectroscopy (FTIR). The surface of the textile
showed noticeable cracks and fissures after
4 months of burial in the bioaugmented model
compost, alongside changes in the functional
groups of the polyamide/polyurethane textile,
which indicates biodegradation of the synthetic
fibers. Searching the genome of Streptomyces sp.
R1, several enzymes involved in the degradation
of synthetic polymers were identified, including
an esterase homologous to highly efficient plastic
degrading depolymerases. Overall, the results
presented here indicate Streptomyces sp. R1 has
the potential for synthetic textile degradation
and bioremediation.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2371"
}

Janković, V., Pantelić, B., Jeremić, S., Radetić, M., Marković, D., Kalogirou, C.,& Ilić-Tomić, T.. (2024). DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2371

Janković V, Pantelić B, Jeremić S, Radetić M, Marković D, Kalogirou C, Ilić-Tomić T. DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2371 .

Janković, Vukašin, Pantelić, Brana, Jeremić, Sanja, Radetić, Maja, Marković, Darka, Kalogirou, Charalampia, Ilić-Tomić, Tatjana, "DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2371 .

TY  - CONF
AU  - Galić, Ivana
AU  - Bez, Christina
AU  - Bertani, Iris
AU  - Simić, Milena
AU  - Venturi, Vittorio
AU  - Stanković, Nada
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2370
AB  - The soil microbiome controls key functions in
agroecosystems determining soil fertility, crop
productivity, and stress tolerance. Crop rotation
is one of the oldest agricultural practices that has
a positive effect on soil quality and the control of
weeds, pests, and pathogens. New insights into
soil microbiome confirm the positive influence of
crop rotation on the diversity of the microbiome.
This study investigates the effects of different
crop rotations under conventional fertilization/
weed management practices on soil microbial
diversity and community structure in two of the
most commonly grown crops in Serbia. The study
investigated the bacterial population in samples
from long-term cultivation experiment of maize
continuous cropping (M-CC) and maize-winter
wheat rotation (M-WW) at two-time points
(December 2022 and May 2023). The results of
16S rDNA amplicon community profiling and
beta diversity analysis showed clear clustering
depending on season, cropping sequence, and
herbicide application. The results show that the
composition of the bacterial community in soil
is largely influenced by seasonal changes. When
comparing bacterial communities in the same
season (May), we observed a significantly higher
biodiversity in M-CC compared to M-WW soils,
suggesting that type of agricultural practice has
a significant impact on the composition of the
microbiome by influencing selection and survival
of specific microbial taxa. In addition, a significant
shift was observed between the bacterial
community composition in M-CC samples under
herbicide treatment and in control soils in December,
while the M-WW community was stable
under all conditions tested. Taxonomically, Actinobacteria
dominated the soil microbiome under
all conditions (53%), followed by Proteobacteria
(23%), Acidobacteria (15%), and Firmicutes (8%).
Despite fluctuations in relative abundance, some
interesting taxa, including Bacillus, Microlunatus,
and Blastococcus, dominated the soil microbiome
under all conditions. These data provide insights
into microbial dynamics, integrating the cultivation
methods with metagenomic approaches to
evaluate different cropping practices.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION
EP  - 69
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2370
ER  - 

@conference{
author = "Galić, Ivana and Bez, Christina and Bertani, Iris and Simić, Milena and Venturi, Vittorio and Stanković, Nada",
year = "2024",
abstract = "The soil microbiome controls key functions in
agroecosystems determining soil fertility, crop
productivity, and stress tolerance. Crop rotation
is one of the oldest agricultural practices that has
a positive effect on soil quality and the control of
weeds, pests, and pathogens. New insights into
soil microbiome confirm the positive influence of
crop rotation on the diversity of the microbiome.
This study investigates the effects of different
crop rotations under conventional fertilization/
weed management practices on soil microbial
diversity and community structure in two of the
most commonly grown crops in Serbia. The study
investigated the bacterial population in samples
from long-term cultivation experiment of maize
continuous cropping (M-CC) and maize-winter
wheat rotation (M-WW) at two-time points
(December 2022 and May 2023). The results of
16S rDNA amplicon community profiling and
beta diversity analysis showed clear clustering
depending on season, cropping sequence, and
herbicide application. The results show that the
composition of the bacterial community in soil
is largely influenced by seasonal changes. When
comparing bacterial communities in the same
season (May), we observed a significantly higher
biodiversity in M-CC compared to M-WW soils,
suggesting that type of agricultural practice has
a significant impact on the composition of the
microbiome by influencing selection and survival
of specific microbial taxa. In addition, a significant
shift was observed between the bacterial
community composition in M-CC samples under
herbicide treatment and in control soils in December,
while the M-WW community was stable
under all conditions tested. Taxonomically, Actinobacteria
dominated the soil microbiome under
all conditions (53%), followed by Proteobacteria
(23%), Acidobacteria (15%), and Firmicutes (8%).
Despite fluctuations in relative abundance, some
interesting taxa, including Bacillus, Microlunatus,
and Blastococcus, dominated the soil microbiome
under all conditions. These data provide insights
into microbial dynamics, integrating the cultivation
methods with metagenomic approaches to
evaluate different cropping practices.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION",
pages = "69-69",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2370"
}

Galić, I., Bez, C., Bertani, I., Simić, M., Venturi, V.,& Stanković, N.. (2024). SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2370

Galić I, Bez C, Bertani I, Simić M, Venturi V, Stanković N. SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:69-69.
https://hdl.handle.net/21.15107/rcub_imagine_2370 .

Galić, Ivana, Bez, Christina, Bertani, Iris, Simić, Milena, Venturi, Vittorio, Stanković, Nada, "SOIL MICROBIOME DIVERSITY IN MAIZE-WINTER WHEAT CROP ROTATION" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):69-69,
https://hdl.handle.net/21.15107/rcub_imagine_2370 .

TY  - CONF
AU  - Matijašević, Danka
AU  - Pavlović, Vladimir
AU  - Kljajević, Nemanja
AU  - Gardijan, Lazar
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2367
AB  - In last decades, bacterial resistance to first choice
antibiotics has been drastically increasing, therefore,
the research of new antimicrobial substances
is of great importance. This rising problem
with bacterial resistance to existing antibiotics
affects not only the health care institutes but also
food plants. S. aureus and S. enteritidis pose a
number of challenges to the food industry and
cause foodborne illness in humans. In addition,
due to their favourable elemental composition,
oyster mushrooms (Pleurotus sp.) are a reservoir
of bioactive compounds that give them remarkable
antibacterial potential. P. ostreatus is of
great economic importance and is the second
most cultivated edible mushroom, therefore information
about its possible targets on bacterial
cells is of great importance for use as a dietary
supplement or medicinal purposes. The results of
the antibacterial assay showed that tested bacterial
strains were susceptible to the methanol extract of P. ostreatus (PoME), while microbicidal
activity was only detected against Gram-positive
bacteria. Scanning electron microscopy (SEM)
micrographs suggested that extract acted on
cytoplasmic membrane of S. aureus, while the
cell envelope of S. Enteritidis was the most likely
target. Natural extracts may outperform individual
bioactive compounds due to the synergistic
interaction between the metabolites, which can
enhance the effects of the individual components.
Extracts rich in antibacterials are emerging
as alternatives to synthetic antibiotics in the food
and health sectors. Among these, crude mushroom
extracts are particularly sought after for
their diverse bioactive ingredients, as they can
combat resistant strains of bacteria due to their
different targets and modes of action. PoME can
be used as an effective antimicrobial agent, suitable
for applications aiming to eradicate foodborne
pathogens, thus enhancing food safety.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM
EP  - 37
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2367
ER  - 

@conference{
author = "Matijašević, Danka and Pavlović, Vladimir and Kljajević, Nemanja and Gardijan, Lazar",
year = "2024",
abstract = "In last decades, bacterial resistance to first choice
antibiotics has been drastically increasing, therefore,
the research of new antimicrobial substances
is of great importance. This rising problem
with bacterial resistance to existing antibiotics
affects not only the health care institutes but also
food plants. S. aureus and S. enteritidis pose a
number of challenges to the food industry and
cause foodborne illness in humans. In addition,
due to their favourable elemental composition,
oyster mushrooms (Pleurotus sp.) are a reservoir
of bioactive compounds that give them remarkable
antibacterial potential. P. ostreatus is of
great economic importance and is the second
most cultivated edible mushroom, therefore information
about its possible targets on bacterial
cells is of great importance for use as a dietary
supplement or medicinal purposes. The results of
the antibacterial assay showed that tested bacterial
strains were susceptible to the methanol extract of P. ostreatus (PoME), while microbicidal
activity was only detected against Gram-positive
bacteria. Scanning electron microscopy (SEM)
micrographs suggested that extract acted on
cytoplasmic membrane of S. aureus, while the
cell envelope of S. Enteritidis was the most likely
target. Natural extracts may outperform individual
bioactive compounds due to the synergistic
interaction between the metabolites, which can
enhance the effects of the individual components.
Extracts rich in antibacterials are emerging
as alternatives to synthetic antibiotics in the food
and health sectors. Among these, crude mushroom
extracts are particularly sought after for
their diverse bioactive ingredients, as they can
combat resistant strains of bacteria due to their
different targets and modes of action. PoME can
be used as an effective antimicrobial agent, suitable
for applications aiming to eradicate foodborne
pathogens, thus enhancing food safety.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM",
pages = "37-37",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2367"
}

Matijašević, D., Pavlović, V., Kljajević, N.,& Gardijan, L.. (2024). MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 37-37.
https://hdl.handle.net/21.15107/rcub_imagine_2367

Matijašević D, Pavlović V, Kljajević N, Gardijan L. MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:37-37.
https://hdl.handle.net/21.15107/rcub_imagine_2367 .

Matijašević, Danka, Pavlović, Vladimir, Kljajević, Nemanja, Gardijan, Lazar, "MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):37-37,
https://hdl.handle.net/21.15107/rcub_imagine_2367 .

TY  - JOUR
AU  - Ljubobratović, Uroš
AU  - Ardó, László
AU  - Fazekas, Georgina
AU  - Horváth, Zoltán
AU  - Lukić, Jovanka
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2312
AB  - The present study aimed to assess the effects of thermal manipulation on the vitellogenesis and final oocyte maturation (FOM) in pikeperch. Two groups of fish were stocked in two separate tanks of the climate chamber. One group was stocked at 10-month age (THERMAL), while the other was continually kept under stable photothermal conditions until 19-month-age (CONTROL) and then transferred to a climate chamber. The progress of vitellogenesis was assessed via evaluation of the oocyte diameter at the mid- and late-autumn phases, and the gonadosomatic index (GSI) at the late-autumn phase. Finally, females from the CONTROL group were hormonally stimulated before (WARMING) and after (STABLE) increase of the temperature from wintering to spawning, and the FOM progress, ovulation and plasma levels of 17 α, 20β dihydroxy progesterone (DHP) were assessed. Significantly larger oocytes at the mid-autumn phase (878.8 ± 40.1 μm vs 836.5 ± 46.5 μm) as well as the GSI at the end of the autumn phase (10.5 ± 1.7% vs 7.6 ± 1.1%) were noticed in THERMAL fish. Significantly faster FOM was seen in fish under the WARMING post-stimulation regime, and these fish had higher DHP levels at the moment of hormonal stimulation (5.4 ± 1.4 ng/ml vs 3.8 ± 1.2 ng/ml). According to the obtained results, it appears that photothermal induction of fish at a younger stage might have a positive impact on the first spawning, while the WARMING thermal regime seems to be more efficient in stimulating the FOM in fish upon first wintering.
PB  - Czech Academy
of Agricultural Sciences
T2  - Czech Journal of Animal Science
T1  - Effect of thermal management on vitellogenesis and maturation in indoor-reared pikeperch (Sander lucioperca)
EP  - 28
IS  - 1
SP  - 18
VL  - 69
DO  - 10.17221/136/2023-CJAS
ER  - 

@article{
author = "Ljubobratović, Uroš and Ardó, László and Fazekas, Georgina and Horváth, Zoltán and Lukić, Jovanka",
year = "2024",
abstract = "The present study aimed to assess the effects of thermal manipulation on the vitellogenesis and final oocyte maturation (FOM) in pikeperch. Two groups of fish were stocked in two separate tanks of the climate chamber. One group was stocked at 10-month age (THERMAL), while the other was continually kept under stable photothermal conditions until 19-month-age (CONTROL) and then transferred to a climate chamber. The progress of vitellogenesis was assessed via evaluation of the oocyte diameter at the mid- and late-autumn phases, and the gonadosomatic index (GSI) at the late-autumn phase. Finally, females from the CONTROL group were hormonally stimulated before (WARMING) and after (STABLE) increase of the temperature from wintering to spawning, and the FOM progress, ovulation and plasma levels of 17 α, 20β dihydroxy progesterone (DHP) were assessed. Significantly larger oocytes at the mid-autumn phase (878.8 ± 40.1 μm vs 836.5 ± 46.5 μm) as well as the GSI at the end of the autumn phase (10.5 ± 1.7% vs 7.6 ± 1.1%) were noticed in THERMAL fish. Significantly faster FOM was seen in fish under the WARMING post-stimulation regime, and these fish had higher DHP levels at the moment of hormonal stimulation (5.4 ± 1.4 ng/ml vs 3.8 ± 1.2 ng/ml). According to the obtained results, it appears that photothermal induction of fish at a younger stage might have a positive impact on the first spawning, while the WARMING thermal regime seems to be more efficient in stimulating the FOM in fish upon first wintering.",
publisher = "Czech Academy
of Agricultural Sciences",
journal = "Czech Journal of Animal Science",
title = "Effect of thermal management on vitellogenesis and maturation in indoor-reared pikeperch (Sander lucioperca)",
pages = "28-18",
number = "1",
volume = "69",
doi = "10.17221/136/2023-CJAS"
}

Ljubobratović, U., Ardó, L., Fazekas, G., Horváth, Z.,& Lukić, J.. (2024). Effect of thermal management on vitellogenesis and maturation in indoor-reared pikeperch (Sander lucioperca). in Czech Journal of Animal Science
Czech Academy
of Agricultural Sciences., 69(1), 18-28.
https://doi.org/10.17221/136/2023-CJAS

Ljubobratović U, Ardó L, Fazekas G, Horváth Z, Lukić J. Effect of thermal management on vitellogenesis and maturation in indoor-reared pikeperch (Sander lucioperca). in Czech Journal of Animal Science. 2024;69(1):18-28.
doi:10.17221/136/2023-CJAS .

Ljubobratović, Uroš, Ardó, László, Fazekas, Georgina, Horváth, Zoltán, Lukić, Jovanka, "Effect of thermal management on vitellogenesis and maturation in indoor-reared pikeperch (Sander lucioperca)" in Czech Journal of Animal Science, 69, no. 1 (2024):18-28,
https://doi.org/10.17221/136/2023-CJAS . .

TY  - CONF
AU  - Mitrović, Hristina
AU  - Brdarić, Emilija
AU  - Bisenić, Aleksandar
AU  - Jakovljević, Stefan
AU  - Dinić, Miroslav
AU  - Đokić, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Radojević, Dušan
AU  - Golić, Nataša
AU  - Soković Bajić, Svetlana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2389
AB  - Psychobiotics are live bacterial strains impacting the central nervous system, producing
neuroactive substances like GABA. GABA from
microbiota influences neural signals, affecting
neurological parameters, sleep, appetite, mood,
and cognition, traversing the intestinal barrier to bind to receptors on enteric neurons and
the vagus nerve. Lactobacillus and Bifidobacterium species can synthesize GABA from dietary
glutamate, with Lactobacillus rhamnosus shown
to reduce anxiety and depressive behavior, elevating hippocampal GABA. Limited knowledge
exists about anaerobic GABA producers, warranting further research for a comprehensive
understanding. Material for isolation comprised
fecal samples from healthy donors, with isolation conducted in an anaerobic chamber within
a maximum of 1 hour after sampling. Isolated
bacteria were identified through sequencing
the 16S rRNA gene. For bacterial cultivation, different types of media were used. PYG medium
contains hematine and vitamin K, essential supplements for the cultivation of anaerobic bacteria. All media included 0.1% L-cysteine, playing a
role in oxygen reduction, and 0.5% glutamate, a
precursor for GABA production. After identification, the presence of GABA in 8 tested bacterial
species was determined using the TLC method.
Quantification of GABA was performed using the
HPLC method. Furthermore, the positive effects
observed in Caco2 cells with induced inflammation, after treatment with certain anaerobic postbiotics producing GABA, indicate the potential
anti-inflammatory effects of these postbiotics.
The study implies anti-inflammatory effects of
anaerobic GABA producers, offering insights into the complex interplay among gut microbiota,
immune function, and mental health. Recognizing inflammation’s role in depressive symptoms,
targeting anaerobic bacteria involved in GABA
synthesis could modulate neurotransmitters and
inflammatory responses, presenting a comprehensive approach to mental well-being. Advancing research in this area contributes to a holistic
understanding of anaerobic bacteria, GABA production, gut microbiota, and mental health. This
offers avenues for novel therapeutic approaches
and enhances overall quality of life.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA
EP  - 111
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2389
ER  - 

@conference{
author = "Mitrović, Hristina and Brdarić, Emilija and Bisenić, Aleksandar and Jakovljević, Stefan and Dinić, Miroslav and Đokić, Jelena and Terzić-Vidojević, Amarela and Tolinački, Maja and Radojević, Dušan and Golić, Nataša and Soković Bajić, Svetlana",
year = "2024",
abstract = "Psychobiotics are live bacterial strains impacting the central nervous system, producing
neuroactive substances like GABA. GABA from
microbiota influences neural signals, affecting
neurological parameters, sleep, appetite, mood,
and cognition, traversing the intestinal barrier to bind to receptors on enteric neurons and
the vagus nerve. Lactobacillus and Bifidobacterium species can synthesize GABA from dietary
glutamate, with Lactobacillus rhamnosus shown
to reduce anxiety and depressive behavior, elevating hippocampal GABA. Limited knowledge
exists about anaerobic GABA producers, warranting further research for a comprehensive
understanding. Material for isolation comprised
fecal samples from healthy donors, with isolation conducted in an anaerobic chamber within
a maximum of 1 hour after sampling. Isolated
bacteria were identified through sequencing
the 16S rRNA gene. For bacterial cultivation, different types of media were used. PYG medium
contains hematine and vitamin K, essential supplements for the cultivation of anaerobic bacteria. All media included 0.1% L-cysteine, playing a
role in oxygen reduction, and 0.5% glutamate, a
precursor for GABA production. After identification, the presence of GABA in 8 tested bacterial
species was determined using the TLC method.
Quantification of GABA was performed using the
HPLC method. Furthermore, the positive effects
observed in Caco2 cells with induced inflammation, after treatment with certain anaerobic postbiotics producing GABA, indicate the potential
anti-inflammatory effects of these postbiotics.
The study implies anti-inflammatory effects of
anaerobic GABA producers, offering insights into the complex interplay among gut microbiota,
immune function, and mental health. Recognizing inflammation’s role in depressive symptoms,
targeting anaerobic bacteria involved in GABA
synthesis could modulate neurotransmitters and
inflammatory responses, presenting a comprehensive approach to mental well-being. Advancing research in this area contributes to a holistic
understanding of anaerobic bacteria, GABA production, gut microbiota, and mental health. This
offers avenues for novel therapeutic approaches
and enhances overall quality of life.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA",
pages = "111-111",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2389"
}

Mitrović, H., Brdarić, E., Bisenić, A., Jakovljević, S., Dinić, M., Đokić, J., Terzić-Vidojević, A., Tolinački, M., Radojević, D., Golić, N.,& Soković Bajić, S.. (2024). FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 111-111.
https://hdl.handle.net/21.15107/rcub_imagine_2389

Mitrović H, Brdarić E, Bisenić A, Jakovljević S, Dinić M, Đokić J, Terzić-Vidojević A, Tolinački M, Radojević D, Golić N, Soković Bajić S. FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:111-111.
https://hdl.handle.net/21.15107/rcub_imagine_2389 .

Mitrović, Hristina, Brdarić, Emilija, Bisenić, Aleksandar, Jakovljević, Stefan, Dinić, Miroslav, Đokić, Jelena, Terzić-Vidojević, Amarela, Tolinački, Maja, Radojević, Dušan, Golić, Nataša, Soković Bajić, Svetlana, "FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):111-111,
https://hdl.handle.net/21.15107/rcub_imagine_2389 .

TY  - JOUR
AU  - Brkušanin, Miloš
AU  - Kosać, Ana
AU  - Branković-Srećković, Vesna
AU  - Jovanović, Kristina
AU  - Perić, Stojan
AU  - Karanović, Jelena
AU  - Matijašević Joković, Suzana
AU  - Garai, Nemanja
AU  - Pešović, Jovan
AU  - Nikolić, Dimitrije
AU  - Stević, Zorica
AU  - Brajušković, Goran
AU  - Milić-Rašić, Vedrana
AU  - Savić-Pavićević, Dušanka
PY  - 2024
UR  - https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2024.1394001/full
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2388
AB  - IntroductionBiomarkers capable of reflecting disease onset and short- and long-term therapeutic effects in individuals with spinal muscular atrophy (SMA) are still an unmet need and phosphorylated neurofilament heavy chain (pNF-H) holds significant promise.MethodsWe conducted a longitudinal prospective study to evaluate pNF-H levels in the cerebrospinal fluid (CSF) and plasma of 29 individuals with childhood-onset SMA treated with Nuinersen (SMA type 1: n = 6, 2: n = 17, 3: n = 6). pNF-H levels before and during treatment were compared with the levels of controls (n = 22), patients with Duchenne muscular dystrophy (n = 17), myotonic dystrophy type 1 (n = 11), untreated SMA individuals with chronic type 3 disease (n = 8), and children with presymptomatic SMA (n = 3).ResultsSMA type 1 showed the highest mean CSF pNF-H levels before treatment initiation. All Nusinersen-treated individuals (types 1, 2, and 3) showed significantly elevated mean baseline CSF pNF-H compared to controls, which inversely correlated with age at disease onset, age at first dose, disease duration and the initial CHOP INTEND result (SMA type 1 and 2). During 22 months of treatment, CSF pNF-H levels declined during loading doses, stabilizing at reduced levels from the initial maintenance dose in all individuals. Baseline plasma pNF-H levels in type 1 and 2 SMA were significantly increased compared to other cohorts and decreased notably in type 1 after 2 months of treatment and type 2 after 14 months. Conversely, SMA type 3, characterized by lower baseline pNF-H levels, did not show significant fluctuations in plasma pNF-H levels after 14 months of treatment.ConclusionOur findings suggest that CSF pNF-H levels in untreated SMA individuals are significantly higher than in controls and that monitoring of CSF pNF-H levels may serve as an indicator of rapid short-term treatment response in childhood-onset SMA individuals, irrespective of the subtype of the disease, while also suggesting its potential for assessing long-term suppression of neurodegeneration. Plasma pNF-H may serve as an appropriate outcome measure for disease progression and/or response to treatment in types 1 and 2 but not in type 3. Presymptomatic infants with SMA may show elevated pNF-H levels, confirming early neuronal degeneration.
PB  - Frontiers
T2  - Frontiers in Neurology
T1  - Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia
VL  - 15
DO  - 10.3389/fneur.2024.1394001
ER  - 

@article{
author = "Brkušanin, Miloš and Kosać, Ana and Branković-Srećković, Vesna and Jovanović, Kristina and Perić, Stojan and Karanović, Jelena and Matijašević Joković, Suzana and Garai, Nemanja and Pešović, Jovan and Nikolić, Dimitrije and Stević, Zorica and Brajušković, Goran and Milić-Rašić, Vedrana and Savić-Pavićević, Dušanka",
year = "2024",
abstract = "IntroductionBiomarkers capable of reflecting disease onset and short- and long-term therapeutic effects in individuals with spinal muscular atrophy (SMA) are still an unmet need and phosphorylated neurofilament heavy chain (pNF-H) holds significant promise.MethodsWe conducted a longitudinal prospective study to evaluate pNF-H levels in the cerebrospinal fluid (CSF) and plasma of 29 individuals with childhood-onset SMA treated with Nuinersen (SMA type 1: n = 6, 2: n = 17, 3: n = 6). pNF-H levels before and during treatment were compared with the levels of controls (n = 22), patients with Duchenne muscular dystrophy (n = 17), myotonic dystrophy type 1 (n = 11), untreated SMA individuals with chronic type 3 disease (n = 8), and children with presymptomatic SMA (n = 3).ResultsSMA type 1 showed the highest mean CSF pNF-H levels before treatment initiation. All Nusinersen-treated individuals (types 1, 2, and 3) showed significantly elevated mean baseline CSF pNF-H compared to controls, which inversely correlated with age at disease onset, age at first dose, disease duration and the initial CHOP INTEND result (SMA type 1 and 2). During 22 months of treatment, CSF pNF-H levels declined during loading doses, stabilizing at reduced levels from the initial maintenance dose in all individuals. Baseline plasma pNF-H levels in type 1 and 2 SMA were significantly increased compared to other cohorts and decreased notably in type 1 after 2 months of treatment and type 2 after 14 months. Conversely, SMA type 3, characterized by lower baseline pNF-H levels, did not show significant fluctuations in plasma pNF-H levels after 14 months of treatment.ConclusionOur findings suggest that CSF pNF-H levels in untreated SMA individuals are significantly higher than in controls and that monitoring of CSF pNF-H levels may serve as an indicator of rapid short-term treatment response in childhood-onset SMA individuals, irrespective of the subtype of the disease, while also suggesting its potential for assessing long-term suppression of neurodegeneration. Plasma pNF-H may serve as an appropriate outcome measure for disease progression and/or response to treatment in types 1 and 2 but not in type 3. Presymptomatic infants with SMA may show elevated pNF-H levels, confirming early neuronal degeneration.",
publisher = "Frontiers",
journal = "Frontiers in Neurology",
title = "Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia",
volume = "15",
doi = "10.3389/fneur.2024.1394001"
}

Brkušanin, M., Kosać, A., Branković-Srećković, V., Jovanović, K., Perić, S., Karanović, J., Matijašević Joković, S., Garai, N., Pešović, J., Nikolić, D., Stević, Z., Brajušković, G., Milić-Rašić, V.,& Savić-Pavićević, D.. (2024). Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia. in Frontiers in Neurology
Frontiers., 15.
https://doi.org/10.3389/fneur.2024.1394001

Brkušanin M, Kosać A, Branković-Srećković V, Jovanović K, Perić S, Karanović J, Matijašević Joković S, Garai N, Pešović J, Nikolić D, Stević Z, Brajušković G, Milić-Rašić V, Savić-Pavićević D. Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia. in Frontiers in Neurology. 2024;15.
doi:10.3389/fneur.2024.1394001 .

Brkušanin, Miloš, Kosać, Ana, Branković-Srećković, Vesna, Jovanović, Kristina, Perić, Stojan, Karanović, Jelena, Matijašević Joković, Suzana, Garai, Nemanja, Pešović, Jovan, Nikolić, Dimitrije, Stević, Zorica, Brajušković, Goran, Milić-Rašić, Vedrana, Savić-Pavićević, Dušanka, "Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia" in Frontiers in Neurology, 15 (2024),
https://doi.org/10.3389/fneur.2024.1394001 . .

TY  - JOUR
AU  - Prlainović, Nevena
AU  - Milovanović, Jelena
AU  - Milašinović, Nikola
AU  - Bezbradica, Dejan
AU  - Mijin, Dušan
PY  - 2024
UR  - https://doiserbia.nb.rs/Article.aspx?ID=0367-598X2400003P
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2311
AB  - Lipase-catalyzed organic reactions have been widely practiced in the past three decades. Especially interesting are insoluble/immobilized forms due to providing a possibility of facile use and recyclability, thus reducing process costs, and making the procedure more environmentally friendly. Carbon-based supports have been extensively exploited for this purpose, because of neutral and biodegradable nature and thermal and chemical stability. Their high specific surface area, characteristic surface morphology and lower mass transfer resistances play a vital role in the performance of the attached enzyme. This review paper presents an overview of the main aspects of lipase immobilized on multi-walled carbon nanotubes (MWCNTs). Moreover, different immobilization strategies to achieve a biocatalyst with improved performances are discussed. Furthermore, as lipases are considered to have high commercial worth for synthesis of valuable organic molecules, the second part of the paper is dedicated to the overview of the most important industrial sectors in which these nanobiocatalysts have been used. In specific, applications in biodiesel production, flavour ester synthesis and racemization are summarized.
AB  - Lipaze su poslednjih decenija široko rasprostanjeni katalizatoriu raznovrsnim organskim reakcijama. Posebno su interesantne u imobilisanom/nerastvornom obliku jer je na ovaj način olakšana njihova upotreba uz mogućnost recikliranja i ponovne upotrebe čime se smanjuju troškovi samog procesa i postupak je ekološki prihvatljiviji. Kao nosači za vezivanje nanomaterijali na bazi ugljenika, posebno ugljenične nanocevi, su našli primenu zbog svojih izuzetnih fizičkih, mehaničkih i hemijskih svojstava. Njihova velika specifična površina, karakteristična površinska morfologija i smanjen otpor prenosu mase igraju vitalnu ulogu u performansama vezanog enzima. Ovaj pregledni rad predstavlja prikaz glavnih aspekata lipaze imobilisane na višeslojne ugljenične nanocevi i različitih strategija imobilizacije za dobijanje biokatalizatora sa poboljšanim svojstvima. Takođe, kako su lipaze enzimi od velikog komercijalnog značaja za organsku sintezu i primenu u biotehnologiji, drugi deo rada posvećen je pregledu najvažnijih industrijskih sektora u kojima su ovi nanobiokatalizatori našli primenu. Shodno tome, dat je pregled proizvodnje biodizela, mirisnih estara i racemizacije
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T2  - Hemijska industrija
T1  - Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update
T1  - Višeslojne ugljenične nanocevi kao nosač lipaze za organsku sintezu: pregled najnovijih trendova
IS  - n/a
DO  - 10.2298/HEMIND230614003P
ER  - 

@article{
author = "Prlainović, Nevena and Milovanović, Jelena and Milašinović, Nikola and Bezbradica, Dejan and Mijin, Dušan",
year = "2024",
abstract = "Lipase-catalyzed organic reactions have been widely practiced in the past three decades. Especially interesting are insoluble/immobilized forms due to providing a possibility of facile use and recyclability, thus reducing process costs, and making the procedure more environmentally friendly. Carbon-based supports have been extensively exploited for this purpose, because of neutral and biodegradable nature and thermal and chemical stability. Their high specific surface area, characteristic surface morphology and lower mass transfer resistances play a vital role in the performance of the attached enzyme. This review paper presents an overview of the main aspects of lipase immobilized on multi-walled carbon nanotubes (MWCNTs). Moreover, different immobilization strategies to achieve a biocatalyst with improved performances are discussed. Furthermore, as lipases are considered to have high commercial worth for synthesis of valuable organic molecules, the second part of the paper is dedicated to the overview of the most important industrial sectors in which these nanobiocatalysts have been used. In specific, applications in biodiesel production, flavour ester synthesis and racemization are summarized., Lipaze su poslednjih decenija široko rasprostanjeni katalizatoriu raznovrsnim organskim reakcijama. Posebno su interesantne u imobilisanom/nerastvornom obliku jer je na ovaj način olakšana njihova upotreba uz mogućnost recikliranja i ponovne upotrebe čime se smanjuju troškovi samog procesa i postupak je ekološki prihvatljiviji. Kao nosači za vezivanje nanomaterijali na bazi ugljenika, posebno ugljenične nanocevi, su našli primenu zbog svojih izuzetnih fizičkih, mehaničkih i hemijskih svojstava. Njihova velika specifična površina, karakteristična površinska morfologija i smanjen otpor prenosu mase igraju vitalnu ulogu u performansama vezanog enzima. Ovaj pregledni rad predstavlja prikaz glavnih aspekata lipaze imobilisane na višeslojne ugljenične nanocevi i različitih strategija imobilizacije za dobijanje biokatalizatora sa poboljšanim svojstvima. Takođe, kako su lipaze enzimi od velikog komercijalnog značaja za organsku sintezu i primenu u biotehnologiji, drugi deo rada posvećen je pregledu najvažnijih industrijskih sektora u kojima su ovi nanobiokatalizatori našli primenu. Shodno tome, dat je pregled proizvodnje biodizela, mirisnih estara i racemizacije",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija, Hemijska industrija",
title = "Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update, Višeslojne ugljenične nanocevi kao nosač lipaze za organsku sintezu: pregled najnovijih trendova",
number = "n/a",
doi = "10.2298/HEMIND230614003P"
}

Prlainović, N., Milovanović, J., Milašinović, N., Bezbradica, D.,& Mijin, D.. (2024). Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update. in Hemijska industrija
Association of the Chemical Engineers of Serbia.(n/a).
https://doi.org/10.2298/HEMIND230614003P

Prlainović N, Milovanović J, Milašinović N, Bezbradica D, Mijin D. Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update. in Hemijska industrija. 2024;(n/a).
doi:10.2298/HEMIND230614003P .

Prlainović, Nevena, Milovanović, Jelena, Milašinović, Nikola, Bezbradica, Dejan, Mijin, Dušan, "Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update" in Hemijska industrija, no. n/a (2024),
https://doi.org/10.2298/HEMIND230614003P . .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Đurašković, Nikola
AU  - Tica, Nedeljko
AU  - Živković, Irena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2334
AB  - In this paper, the biodegradability of three SferiCorn™
biocomposites was investigated. Corn stover prepared through eco-friendly
washing and grinding to short fibers was used as reinforcement. Three different
biopolymers, corn starch, alginate and poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) (PHBV), were used as matrices. The biodegradability of
prepared composites was investigated in a simulated soil burial test and the
samples were characterized in terms of weight loss and morphological changes. It
was shown that biodegradability can be tuned by biopolymers used as matrices.
Changes in the surface morphology after biodegradation of tested samples were
confirmed using scanning electron microscopy (SEM).
PB  - Čačak : Faculty of Agronomy
C3  - 2nd International symposium on biotechnology
T1  - Biodegradability assessment of corn stover reinforced composite materials with different matrix
EP  - 261
SP  - 225
DO  - 10.46793/SBT29
ER  - 

@conference{
author = "Ponjavić, Marijana and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Đurašković, Nikola and Tica, Nedeljko and Živković, Irena",
year = "2024",
abstract = "In this paper, the biodegradability of three SferiCorn™
biocomposites was investigated. Corn stover prepared through eco-friendly
washing and grinding to short fibers was used as reinforcement. Three different
biopolymers, corn starch, alginate and poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) (PHBV), were used as matrices. The biodegradability of
prepared composites was investigated in a simulated soil burial test and the
samples were characterized in terms of weight loss and morphological changes. It
was shown that biodegradability can be tuned by biopolymers used as matrices.
Changes in the surface morphology after biodegradation of tested samples were
confirmed using scanning electron microscopy (SEM).",
publisher = "Čačak : Faculty of Agronomy",
journal = "2nd International symposium on biotechnology",
title = "Biodegradability assessment of corn stover reinforced composite materials with different matrix",
pages = "261-225",
doi = "10.46793/SBT29"
}

Ponjavić, M., Milovanović, J., Nikodinović-Runić, J., Đurašković, N., Tica, N.,& Živković, I.. (2024). Biodegradability assessment of corn stover reinforced composite materials with different matrix. in 2nd International symposium on biotechnology
Čačak : Faculty of Agronomy., 225-261.
https://doi.org/10.46793/SBT29

Ponjavić M, Milovanović J, Nikodinović-Runić J, Đurašković N, Tica N, Živković I. Biodegradability assessment of corn stover reinforced composite materials with different matrix. in 2nd International symposium on biotechnology. 2024;:225-261.
doi:10.46793/SBT29 .

Ponjavić, Marijana, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Đurašković, Nikola, Tica, Nedeljko, Živković, Irena, "Biodegradability assessment of corn stover reinforced composite materials with different matrix" in 2nd International symposium on biotechnology (2024):225-261,
https://doi.org/10.46793/SBT29 . .

TY  - CONF
AU  - Petrović, Jelena
AU  - Pańczyszyn, Elżbieta
AU  - Corazzari, Marco
AU  - Banićević, Ivana
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Stevanović, Milena
AU  - Dragoj, Miodrag
AU  - Pešić, Milica
AU  - Janković, Radmila
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1264
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2364
AB  - The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.
C3  - Hemijska industrija (Chemical Industry)
T1  - Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model
EP  - 21
IS  - 1S
SP  - 21
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2364
ER  - 

@conference{
author = "Petrović, Jelena and Pańczyszyn, Elżbieta and Corazzari, Marco and Banićević, Ivana and Milivojević, Milena and Bojić, Luka and Stevanović, Milena and Dragoj, Miodrag and Pešić, Milica and Janković, Radmila and Obradović, Bojana and Stojkovska, Jasmina",
year = "2024",
abstract = "The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model",
pages = "21-21",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2364"
}

Petrović, J., Pańczyszyn, E., Corazzari, M., Banićević, I., Milivojević, M., Bojić, L., Stevanović, M., Dragoj, M., Pešić, M., Janković, R., Obradović, B.,& Stojkovska, J.. (2024). Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry), 78(1S), 21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364

Petrović J, Pańczyszyn E, Corazzari M, Banićević I, Milivojević M, Bojić L, Stevanović M, Dragoj M, Pešić M, Janković R, Obradović B, Stojkovska J. Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry). 2024;78(1S):21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

Petrović, Jelena, Pańczyszyn, Elżbieta, Corazzari, Marco, Banićević, Ivana, Milivojević, Milena, Bojić, Luka, Stevanović, Milena, Dragoj, Miodrag, Pešić, Milica, Janković, Radmila, Obradović, Bojana, Stojkovska, Jasmina, "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):21-21,
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

TY  - GEN
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2307
AB  - In recent years, data-driven medicine has gained increasing importance in terms of diagnosis,

treatment, and research due to the exponential growth of healthcare data. The linkage of cross-
border health data from various sources, including genomics, and analysis via innovative

approaches based on artificial intelligence (AI) will enable a better understanding of risk factors,
causes, and the development of optimal treatment in different disease areas. Nevertheless, the
reuse of patient data is often limited to datasets available at a single medical centre. The main
reasons why health data is not shared across institutional borders rely on ethical, legal, and privacy
aspects and rules. Therefore, in order to (1) enable health data sharing across national borders,
(2) fully comply with present GDPR privacy guidelines / regulations and (3) innovate by pushing
research beyond the state of the art, BETTER proposes a robust decentralised privacy-preserving
infrastructure which will empower researchers, innovators and healthcare professionals to exploit
the full potential of larger sets of multi-source health data via tailored made AI tools useful to
compare, integrate, and analyse in a secure, cost-effective fashion; with the very final aim of
supporting the improvement of citizen’s health outcomes. In detail, this interdisciplinary project
proposes the co-creation of 3 clinical use cases involving 7 medical centres located in the EU and
beyond, where sensitive patient data, including genomics, are made available and analysed in a
GDPR-compliant mechanism via a Distributed Analytics (DA) paradigm called the Personal Health
Train (PHT). The main principle of the PHT is that the analytical task is brought to the data provider
(medical centre) and the data instances remain in their original location. In this project, two
mature implementations of the PHT (PADME and Vantage6) already validated in real-world
scenarios will be fused together to build the BETTER platform.
T2  - European Comission, Horizon Europe
T1  - Better real-world health-data distributed analytics research platform
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2307
ER  - 

@misc{
year = "2024",
abstract = "In recent years, data-driven medicine has gained increasing importance in terms of diagnosis,

treatment, and research due to the exponential growth of healthcare data. The linkage of cross-
border health data from various sources, including genomics, and analysis via innovative

approaches based on artificial intelligence (AI) will enable a better understanding of risk factors,
causes, and the development of optimal treatment in different disease areas. Nevertheless, the
reuse of patient data is often limited to datasets available at a single medical centre. The main
reasons why health data is not shared across institutional borders rely on ethical, legal, and privacy
aspects and rules. Therefore, in order to (1) enable health data sharing across national borders,
(2) fully comply with present GDPR privacy guidelines / regulations and (3) innovate by pushing
research beyond the state of the art, BETTER proposes a robust decentralised privacy-preserving
infrastructure which will empower researchers, innovators and healthcare professionals to exploit
the full potential of larger sets of multi-source health data via tailored made AI tools useful to
compare, integrate, and analyse in a secure, cost-effective fashion; with the very final aim of
supporting the improvement of citizen’s health outcomes. In detail, this interdisciplinary project
proposes the co-creation of 3 clinical use cases involving 7 medical centres located in the EU and
beyond, where sensitive patient data, including genomics, are made available and analysed in a
GDPR-compliant mechanism via a Distributed Analytics (DA) paradigm called the Personal Health
Train (PHT). The main principle of the PHT is that the analytical task is brought to the data provider
(medical centre) and the data instances remain in their original location. In this project, two
mature implementations of the PHT (PADME and Vantage6) already validated in real-world
scenarios will be fused together to build the BETTER platform.",
journal = "European Comission, Horizon Europe",
title = "Better real-world health-data distributed analytics research platform",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2307"
}

(2024). Better real-world health-data distributed analytics research platform. in European Comission, Horizon Europe.
https://hdl.handle.net/21.15107/rcub_imagine_2307

Better real-world health-data distributed analytics research platform. in European Comission, Horizon Europe. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2307 .

"Better real-world health-data distributed analytics research platform" in European Comission, Horizon Europe (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2307 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Milošević, Mia
AU  - Petrović, Jelena
AU  - Menshikh, Ksenia
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Janković, Radmila
AU  - Cochis, Andrea
AU  - Bella, Elena Della
AU  - Stoddart, Martin
AU  - Rimondini, Lia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1261
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2363
AB  - Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.
C3  - Hemijska industrija (Chemical Industry)
T1  - A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research
EP  - 19
IS  - 1S
SP  - 19
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2363
ER  - 

@conference{
author = "Banićević, Ivana and Milošević, Mia and Petrović, Jelena and Menshikh, Ksenia and Milivojević, Milena and Stevanović, Milena and Janković, Radmila and Cochis, Andrea and Bella, Elena Della and Stoddart, Martin and Rimondini, Lia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2024",
abstract = "Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research",
pages = "19-19",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2363"
}

Banićević, I., Milošević, M., Petrović, J., Menshikh, K., Milivojević, M., Stevanović, M., Janković, R., Cochis, A., Bella, E. D., Stoddart, M., Rimondini, L., Stojkovska, J.,& Obradović, B.. (2024). A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry), 78(1S), 19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363

Banićević I, Milošević M, Petrović J, Menshikh K, Milivojević M, Stevanović M, Janković R, Cochis A, Bella ED, Stoddart M, Rimondini L, Stojkovska J, Obradović B. A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry). 2024;78(1S):19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

Banićević, Ivana, Milošević, Mia, Petrović, Jelena, Menshikh, Ksenia, Milivojević, Milena, Stevanović, Milena, Janković, Radmila, Cochis, Andrea, Bella, Elena Della, Stoddart, Martin, Rimondini, Lia, Stojkovska, Jasmina, Obradović, Bojana, "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):19-19,
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

TY  - CONF
AU  - Stanisavljević, Nemanja
AU  - Malešević, Milka
AU  - Ćurčić, Jovana
AU  - Matijašević, Danka
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Gašić, Uroš
AU  - Pešić, Mirjana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2382
AB  - During the past decades several quorum sensing
inhibitors (QSI) of plant origin have been isolated
and chemically characterized. QSI agents
of plant origin represent potential alternative
or complementary approach to antibiotic treatment
of multidrug-resistant bacteria and infections
caused by bacterial biofilms. The aim of
the current study was to screen QSI activities
of horseradish root extracts obtained using
different organic solvents and different root
processing methods (drying at 40°C, 60°C or extraction
of fresh material). Common opportunistic
pathogen Pseudomonas aeruginosa MMA83
was used for QSI screen. RT-qPCR was used to
analyze the effect of the extract on the relative
mRNA levels of the genes QS (lasR, lasI, rhlR, rhlI,
mvfR, pqsH) and the genes involved in P. aeruginosa
MMA83 virulence (lasB, phzM, rhlC, algK,
pvdS). Chemical composition of extracts was
determined by UHPLC Q-ToF MS analysis. The
most active extract obtained using fresh roots
and hexane/ethyl acetate (1:1) solvent mixture
was able to significantly reduce content all examined
mRNA. Qualitative chemical analysis
reviled presence of 15 phenolic acids and their
derivatives, 9 flavonoids and 10 glucosinolates
in majority of examined extracts. It is significant
to emphasize that the most active QSI extract
did not contain a single one, out of ten dominant
glucosinolates, which have undergone to
hydrolysis yielding isothiocyanates and other
sulphur-containing compounds responsible for
QSI effects. Our results strongly indicate that
even mild thermal treatment (40°C) of horseradish
roots prior to extraction could lead to severe
reduction or loss of QSI activity.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS
EP  - 147
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2382
ER  - 

@conference{
author = "Stanisavljević, Nemanja and Malešević, Milka and Ćurčić, Jovana and Matijašević, Danka and Kostić, Aleksandar and Milinčić, Danijel and Gašić, Uroš and Pešić, Mirjana",
year = "2024",
abstract = "During the past decades several quorum sensing
inhibitors (QSI) of plant origin have been isolated
and chemically characterized. QSI agents
of plant origin represent potential alternative
or complementary approach to antibiotic treatment
of multidrug-resistant bacteria and infections
caused by bacterial biofilms. The aim of
the current study was to screen QSI activities
of horseradish root extracts obtained using
different organic solvents and different root
processing methods (drying at 40°C, 60°C or extraction
of fresh material). Common opportunistic
pathogen Pseudomonas aeruginosa MMA83
was used for QSI screen. RT-qPCR was used to
analyze the effect of the extract on the relative
mRNA levels of the genes QS (lasR, lasI, rhlR, rhlI,
mvfR, pqsH) and the genes involved in P. aeruginosa
MMA83 virulence (lasB, phzM, rhlC, algK,
pvdS). Chemical composition of extracts was
determined by UHPLC Q-ToF MS analysis. The
most active extract obtained using fresh roots
and hexane/ethyl acetate (1:1) solvent mixture
was able to significantly reduce content all examined
mRNA. Qualitative chemical analysis
reviled presence of 15 phenolic acids and their
derivatives, 9 flavonoids and 10 glucosinolates
in majority of examined extracts. It is significant
to emphasize that the most active QSI extract
did not contain a single one, out of ten dominant
glucosinolates, which have undergone to
hydrolysis yielding isothiocyanates and other
sulphur-containing compounds responsible for
QSI effects. Our results strongly indicate that
even mild thermal treatment (40°C) of horseradish
roots prior to extraction could lead to severe
reduction or loss of QSI activity.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2382"
}

Stanisavljević, N., Malešević, M., Ćurčić, J., Matijašević, D., Kostić, A., Milinčić, D., Gašić, U.,& Pešić, M.. (2024). CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 147-147.
https://hdl.handle.net/21.15107/rcub_imagine_2382

Stanisavljević N, Malešević M, Ćurčić J, Matijašević D, Kostić A, Milinčić D, Gašić U, Pešić M. CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:147-147.
https://hdl.handle.net/21.15107/rcub_imagine_2382 .

Stanisavljević, Nemanja, Malešević, Milka, Ćurčić, Jovana, Matijašević, Danka, Kostić, Aleksandar, Milinčić, Danijel, Gašić, Uroš, Pešić, Mirjana, "CHEMICAL COMPOSITION AND QUORUM SENSING INHIBITION ACTIVITY OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):147-147,
https://hdl.handle.net/21.15107/rcub_imagine_2382 .

TY  - CONF
AU  - Malešević, Milka
AU  - Ćurčić, Jovana
AU  - Gardijan, Lazar
AU  - Obradović, Mina
AU  - Stanisavljević, Nemanja
AU  - Pantić, Milena
AU  - Matijašević, Danka
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2381
AB  - Pseudomonas aeruginosa has been recognized as
a priority pathogen by World Health Organization,
due to the emergence of multidrug-resistant
(MDR) strains. Thus, new treatment options
such as antivirulence strategy is urgently needed.
This strategy is based on the disruption of quorum
sensing (QS) activity of this pathogen. The
focus of this research was to explore the anti-QS
activity of four selected medicinal mushrooms
(Lentinula edodes, Cantharellus cibarius, Trametes
versicolor and Pleurotus ostreatus) extracts on
MDR clinical isolate P. aeruginosa MMA83. Another
aim was to check their cytotoxicity on Caenorhabditis
elegans AU37 (glp-4(bn2) I; sec-1(km4).
Among three types of mushroom extracts - hot
water polysaccharide extracts (WPE), hot alkali
polysaccharide extracts (APE) and methanol
extracts (Met), APE extracts downregulated all
tested QS and virulence factors genes of P. aeruginosa
MMA83. The most prominent effect was
observed for C. cibarius APE extract, lowering
expression from 2-fold (for lasI gene) to 20-fold
for lasB gene. Extracts didn’t show cytotoxic effect
on C. elegans. The efficacy of APE extracts
in lowering the expression of QS and virulence
factors genes of P. aeruginosa MMA83 indicate
that these extracts can reduce pathogenicity of
P. aeruginosa. Also, they possess one of the desirable
biotechnology features – the absence of
cytotoxicity. Anti-QS and antivirulence effect of
APE extracts on P. aeruginosa envisages these extracts
as the promising therapeutic candidates
for the development of next-generation antivirulence
agents.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE
EP  - 145
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2381
ER  - 

@conference{
author = "Malešević, Milka and Ćurčić, Jovana and Gardijan, Lazar and Obradović, Mina and Stanisavljević, Nemanja and Pantić, Milena and Matijašević, Danka",
year = "2024",
abstract = "Pseudomonas aeruginosa has been recognized as
a priority pathogen by World Health Organization,
due to the emergence of multidrug-resistant
(MDR) strains. Thus, new treatment options
such as antivirulence strategy is urgently needed.
This strategy is based on the disruption of quorum
sensing (QS) activity of this pathogen. The
focus of this research was to explore the anti-QS
activity of four selected medicinal mushrooms
(Lentinula edodes, Cantharellus cibarius, Trametes
versicolor and Pleurotus ostreatus) extracts on
MDR clinical isolate P. aeruginosa MMA83. Another
aim was to check their cytotoxicity on Caenorhabditis
elegans AU37 (glp-4(bn2) I; sec-1(km4).
Among three types of mushroom extracts - hot
water polysaccharide extracts (WPE), hot alkali
polysaccharide extracts (APE) and methanol
extracts (Met), APE extracts downregulated all
tested QS and virulence factors genes of P. aeruginosa
MMA83. The most prominent effect was
observed for C. cibarius APE extract, lowering
expression from 2-fold (for lasI gene) to 20-fold
for lasB gene. Extracts didn’t show cytotoxic effect
on C. elegans. The efficacy of APE extracts
in lowering the expression of QS and virulence
factors genes of P. aeruginosa MMA83 indicate
that these extracts can reduce pathogenicity of
P. aeruginosa. Also, they possess one of the desirable
biotechnology features – the absence of
cytotoxicity. Anti-QS and antivirulence effect of
APE extracts on P. aeruginosa envisages these extracts
as the promising therapeutic candidates
for the development of next-generation antivirulence
agents.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE",
pages = "145-145",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2381"
}

Malešević, M., Ćurčić, J., Gardijan, L., Obradović, M., Stanisavljević, N., Pantić, M.,& Matijašević, D.. (2024). MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 145-145.
https://hdl.handle.net/21.15107/rcub_imagine_2381

Malešević M, Ćurčić J, Gardijan L, Obradović M, Stanisavljević N, Pantić M, Matijašević D. MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:145-145.
https://hdl.handle.net/21.15107/rcub_imagine_2381 .

Malešević, Milka, Ćurčić, Jovana, Gardijan, Lazar, Obradović, Mina, Stanisavljević, Nemanja, Pantić, Milena, Matijašević, Danka, "MEDICINAL MUSHROOM EXTRACTS ATTENUATE PSEUDOMONAS AERUGINOSA QUORUM SENSING AND VIRULENCE" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):145-145,
https://hdl.handle.net/21.15107/rcub_imagine_2381 .

TY  - JOUR
AU  - Tomić Vujović, Kristina
AU  - Ugrin, Milena
AU  - Tošić, Nataša
AU  - Vuković, Vojin
AU  - Marjanović, Irena
AU  - Kostić, Tatjana
AU  - Stanković, Sanja
AU  - Otašević, Vladimir
AU  - Šarac, Sofija
AU  - Antić, Darko
AU  - Pavlović, Sonja
AU  - Karan-Đurašević, Teodora
PY  - 2024
UR  - https://www.mdpi.com/1422-0067/25/2/922
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2310
AB  - Dysregulated expression of the long non-coding RNA MALAT1 has been implicated in the pathogenesis and progression of a variety of cancers, including hematological malignancies, but it has been poorly investigated in chronic lymphocytic leukemia (CLL). In this study, the expression of MALAT1 was measured using a quantitative reverse-transcriptase polymerase chain reaction in the peripheral blood mononuclear cells of 114 unselected, newly diagnosed CLL patients in order to analyze its association with clinical, laboratory, and molecular patients’ characteristics at diagnosis, as well as its prognostic relevance. MALAT1 was found to be upregulated in CLL patients in comparison to healthy controls, and expression levels were not related to age, leukocyte, lymphocyte and platelet count, serum β2-microglobulin, and IGHV somatic hypermutational status. On the other hand, high MALAT1 expression was associated with several favorable prognostic markers (high hemoglobin, low serum lactate dehydrogenase, earlier clinical stages, CD38-negative status), but also with unfavorable cytogenetics. Furthermore, an association between high MALAT1 levels and longer time to first treatment and overall survival in IGHV-unmutated CLL subtype was observed. In summary, our results imply that high MALAT1 expression at diagnosis may be a predictor of better prognosis and point to MALAT1 expression profiling as a candidate biomarker potentially useful in clinical practice.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia
IS  - 2
SP  - 922
VL  - 25
DO  - 10.3390/ijms25020922
ER  - 

@article{
author = "Tomić Vujović, Kristina and Ugrin, Milena and Tošić, Nataša and Vuković, Vojin and Marjanović, Irena and Kostić, Tatjana and Stanković, Sanja and Otašević, Vladimir and Šarac, Sofija and Antić, Darko and Pavlović, Sonja and Karan-Đurašević, Teodora",
year = "2024",
abstract = "Dysregulated expression of the long non-coding RNA MALAT1 has been implicated in the pathogenesis and progression of a variety of cancers, including hematological malignancies, but it has been poorly investigated in chronic lymphocytic leukemia (CLL). In this study, the expression of MALAT1 was measured using a quantitative reverse-transcriptase polymerase chain reaction in the peripheral blood mononuclear cells of 114 unselected, newly diagnosed CLL patients in order to analyze its association with clinical, laboratory, and molecular patients’ characteristics at diagnosis, as well as its prognostic relevance. MALAT1 was found to be upregulated in CLL patients in comparison to healthy controls, and expression levels were not related to age, leukocyte, lymphocyte and platelet count, serum β2-microglobulin, and IGHV somatic hypermutational status. On the other hand, high MALAT1 expression was associated with several favorable prognostic markers (high hemoglobin, low serum lactate dehydrogenase, earlier clinical stages, CD38-negative status), but also with unfavorable cytogenetics. Furthermore, an association between high MALAT1 levels and longer time to first treatment and overall survival in IGHV-unmutated CLL subtype was observed. In summary, our results imply that high MALAT1 expression at diagnosis may be a predictor of better prognosis and point to MALAT1 expression profiling as a candidate biomarker potentially useful in clinical practice.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia",
number = "2",
pages = "922",
volume = "25",
doi = "10.3390/ijms25020922"
}

Tomić Vujović, K., Ugrin, M., Tošić, N., Vuković, V., Marjanović, I., Kostić, T., Stanković, S., Otašević, V., Šarac, S., Antić, D., Pavlović, S.,& Karan-Đurašević, T.. (2024). Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia. in International Journal of Molecular Sciences
MDPI., 25(2), 922.
https://doi.org/10.3390/ijms25020922

Tomić Vujović K, Ugrin M, Tošić N, Vuković V, Marjanović I, Kostić T, Stanković S, Otašević V, Šarac S, Antić D, Pavlović S, Karan-Đurašević T. Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia. in International Journal of Molecular Sciences. 2024;25(2):922.
doi:10.3390/ijms25020922 .

Tomić Vujović, Kristina, Ugrin, Milena, Tošić, Nataša, Vuković, Vojin, Marjanović, Irena, Kostić, Tatjana, Stanković, Sanja, Otašević, Vladimir, Šarac, Sofija, Antić, Darko, Pavlović, Sonja, Karan-Đurašević, Teodora, "Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia" in International Journal of Molecular Sciences, 25, no. 2 (2024):922,
https://doi.org/10.3390/ijms25020922 . .

TY  - JOUR
AU  - Lazić, Jelena
AU  - Filipović, Vuk
AU  - Pantelić, Lena
AU  - Milovanović, Jelena
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2024.1351583
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2387
AB  - Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.
PB  - Frontiers
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Late-stage diversification of bacterial natural products through biocatalysis
VL  - 12
DO  - 10.3389/fbioe.2024.1351583
ER  - 

@article{
author = "Lazić, Jelena and Filipović, Vuk and Pantelić, Lena and Milovanović, Jelena and Vojnović, Sandra and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.",
publisher = "Frontiers",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Late-stage diversification of bacterial natural products through biocatalysis",
volume = "12",
doi = "10.3389/fbioe.2024.1351583"
}

Lazić, J., Filipović, V., Pantelić, L., Milovanović, J., Vojnović, S.,& Nikodinović-Runić, J.. (2024). Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology
Frontiers., 12.
https://doi.org/10.3389/fbioe.2024.1351583

Lazić J, Filipović V, Pantelić L, Milovanović J, Vojnović S, Nikodinović-Runić J. Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology. 2024;12.
doi:10.3389/fbioe.2024.1351583 .

Lazić, Jelena, Filipović, Vuk, Pantelić, Lena, Milovanović, Jelena, Vojnović, Sandra, Nikodinović-Runić, Jasmina, "Late-stage diversification of bacterial natural products through biocatalysis" in Frontiers in Bioengineering and Biotechnology, 12 (2024),
https://doi.org/10.3389/fbioe.2024.1351583 . .

TY  - CONF
AU  - T. Milenković, Marina
AU  - Ušjak, Dušan
AU  - Tadić, Vanja
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2377
AB  - Antimicrobial resistance (AMR) has developed as
one of the top 10 global public health threats
facing humanity. As the nosocomial bacterial
strains are being increasingly resistant to most
clinically available antibiotics, there is a constant
need for exploration of new substances
that could kill them or inhibit their growth, or
alternatively inhibit some of their essential virulence
factors to counteract the lack of new antibacterials
and the rise of antibiotic resistance,
plants could represent a potential solution.
Plants produce a variety of bioactive secondary
metabolites that could be used to fuel the future
discovery pipeline. Aim of the present study was
to examine inhibitory activity of the supercritical
extract of J. communis L. green pseudofructus
(7SCO2) against the growth, biofilm production
and several virulence factors of significant nosocomial
bacterial pathogens. The extract was
obtained by fractional extraction with supercritical
CO2, and the qualitative and quantitative
analysis was performed using the GC-FID/MS
method. Clinical isolates of Pseudomonas aeruginosa,Acinetobacter baumannii, Staphylococcus
aureus (methicillin-sensitive-MSSA and methicillin-
resistant - MRSA), Enterococcus faecalis, and
Klebsiella pneumoniae, as well as their antibiotic
resistance profiles, were obtained from the Clinical
Hospital Centre “Dr Dragiša Mišović Dedinje”.
Minimum inhibitory concentrations (MICs) of
the 7SCO2 were determined by broth-microdilution
method. Examination of the anti-adhesive
effect of the extract was carried out using the
spectrophotometric method. The pyocyanin
production of Pseudomonas aeruginosa was determined
by the method described by Rampioni
et al. Most significant findings of this study
are potent antivirulence activity of the 7SCO2
against P. aeruginosa through the inhibition of
pyocyanin production. In addition, the biofilm
production of A. baumannii was inhibited by the
7SCO2 in concentration 50 μg/mL. Finally, notable
antivirulence activity of the 7SCO2 against
E. faecalis and S. aureus was detected, since it
significantly inhibited collagen and laminin adhesion
of these pathogens.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS
EP  - 131
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2377
ER  - 

@conference{
author = "T. Milenković, Marina and Ušjak, Dušan and Tadić, Vanja",
year = "2024",
abstract = "Antimicrobial resistance (AMR) has developed as
one of the top 10 global public health threats
facing humanity. As the nosocomial bacterial
strains are being increasingly resistant to most
clinically available antibiotics, there is a constant
need for exploration of new substances
that could kill them or inhibit their growth, or
alternatively inhibit some of their essential virulence
factors to counteract the lack of new antibacterials
and the rise of antibiotic resistance,
plants could represent a potential solution.
Plants produce a variety of bioactive secondary
metabolites that could be used to fuel the future
discovery pipeline. Aim of the present study was
to examine inhibitory activity of the supercritical
extract of J. communis L. green pseudofructus
(7SCO2) against the growth, biofilm production
and several virulence factors of significant nosocomial
bacterial pathogens. The extract was
obtained by fractional extraction with supercritical
CO2, and the qualitative and quantitative
analysis was performed using the GC-FID/MS
method. Clinical isolates of Pseudomonas aeruginosa,Acinetobacter baumannii, Staphylococcus
aureus (methicillin-sensitive-MSSA and methicillin-
resistant - MRSA), Enterococcus faecalis, and
Klebsiella pneumoniae, as well as their antibiotic
resistance profiles, were obtained from the Clinical
Hospital Centre “Dr Dragiša Mišović Dedinje”.
Minimum inhibitory concentrations (MICs) of
the 7SCO2 were determined by broth-microdilution
method. Examination of the anti-adhesive
effect of the extract was carried out using the
spectrophotometric method. The pyocyanin
production of Pseudomonas aeruginosa was determined
by the method described by Rampioni
et al. Most significant findings of this study
are potent antivirulence activity of the 7SCO2
against P. aeruginosa through the inhibition of
pyocyanin production. In addition, the biofilm
production of A. baumannii was inhibited by the
7SCO2 in concentration 50 μg/mL. Finally, notable
antivirulence activity of the 7SCO2 against
E. faecalis and S. aureus was detected, since it
significantly inhibited collagen and laminin adhesion
of these pathogens.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2377"
}

T. Milenković, M., Ušjak, D.,& Tadić, V.. (2024). HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 131-131.
https://hdl.handle.net/21.15107/rcub_imagine_2377

T. Milenković M, Ušjak D, Tadić V. HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:131-131.
https://hdl.handle.net/21.15107/rcub_imagine_2377 .

T. Milenković, Marina, Ušjak, Dušan, Tadić, Vanja, "HERBAL PRODUCTS AS AN ALTERNATIVE TO ANTIBIOTICS: APPLICATION POSSIBILITIES AND LIMITATIONS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):131-131,
https://hdl.handle.net/21.15107/rcub_imagine_2377 .

TY  - CONF
AU  - Bisenić, Aleksandar
AU  - Tomić, Sergej
AU  - Bekić, Marina
AU  - Pavlović, Luka
AU  - Dinić, Miroslav
AU  - Terzić- Vidojević, Amarela
AU  - Radojević, Dušan
AU  - Soković Bajić, Svetlana
AU  - Mitrović, Hristina
AU  - Jakovljević, Stefan
AU  - Stevanović, Dušan
AU  - Golić, Nataša
AU  - Đokić, Jelena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2374
AB  - Alterations in gut microbiota and deregulation
of the gut immune system are recognized
as important events in autoimmune diseases.
The knowledge about the important role of anaerobic
gut bacteria that produce short-chain
fatty acids (SCFAs), in the regulation of intestinal
barrier and immune response made a way
for the development of microbiota-based interventions.
Our research aimed to isolate the
strains with the potential to produce SCFAs,
from healthy volunteer fecal material, and to
test their effects on IL-8 production in the culture
of intestinal epithelial cells (Caco2) as an in
vitro system imitating initial intestinal inflammation,
the effects on the expression of neuronal
activity-regulated genes of Caenorhabditis
elegans, and the effect on the development
of experimental autoimmune encephalomyelitis
(EAE), a mouse model of multiple  sclerosis.
Three isolated butyric acid (BA)-producing
strains, and three acetic acid (AA)-producing
strains diminished the production of IL-8 in Caco-
2 cells treated with IL-1β/TNF-α. Further, all
BA-producing strains stimulated the expression
of important neuro-related genes in C. elegans.
Based on the strongest effects in these
assays an isolate identified as Faecalimonas sp.
NGB245 strain was further tested in EAE model.
The oral treatment of EAE-induced mice with
this strain for 16h per day for 15 days resulted
in alleviated daily clinical scores, maximal
clinical scores, and the duration of the illness
in comparison to the effect of media used for
strain cultivation. These results point to the potential
of NGB245 to modify the gut-brain axis
opening the field for future development of microbiota-
based therapy for the diseases associated
with immune response dysfunctions.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
EP  - 116
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2374
ER  - 

@conference{
author = "Bisenić, Aleksandar and Tomić, Sergej and Bekić, Marina and Pavlović, Luka and Dinić, Miroslav and Terzić- Vidojević, Amarela and Radojević, Dušan and Soković Bajić, Svetlana and Mitrović, Hristina and Jakovljević, Stefan and Stevanović, Dušan and Golić, Nataša and Đokić, Jelena",
year = "2024",
abstract = "Alterations in gut microbiota and deregulation
of the gut immune system are recognized
as important events in autoimmune diseases.
The knowledge about the important role of anaerobic
gut bacteria that produce short-chain
fatty acids (SCFAs), in the regulation of intestinal
barrier and immune response made a way
for the development of microbiota-based interventions.
Our research aimed to isolate the
strains with the potential to produce SCFAs,
from healthy volunteer fecal material, and to
test their effects on IL-8 production in the culture
of intestinal epithelial cells (Caco2) as an in
vitro system imitating initial intestinal inflammation,
the effects on the expression of neuronal
activity-regulated genes of Caenorhabditis
elegans, and the effect on the development
of experimental autoimmune encephalomyelitis
(EAE), a mouse model of multiple  sclerosis.
Three isolated butyric acid (BA)-producing
strains, and three acetic acid (AA)-producing
strains diminished the production of IL-8 in Caco-
2 cells treated with IL-1β/TNF-α. Further, all
BA-producing strains stimulated the expression
of important neuro-related genes in C. elegans.
Based on the strongest effects in these
assays an isolate identified as Faecalimonas sp.
NGB245 strain was further tested in EAE model.
The oral treatment of EAE-induced mice with
this strain for 16h per day for 15 days resulted
in alleviated daily clinical scores, maximal
clinical scores, and the duration of the illness
in comparison to the effect of media used for
strain cultivation. These results point to the potential
of NGB245 to modify the gut-brain axis
opening the field for future development of microbiota-
based therapy for the diseases associated
with immune response dysfunctions.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS",
pages = "116-116",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2374"
}

Bisenić, A., Tomić, S., Bekić, M., Pavlović, L., Dinić, M., Terzić- Vidojević, A., Radojević, D., Soković Bajić, S., Mitrović, H., Jakovljević, S., Stevanović, D., Golić, N.,& Đokić, J.. (2024). SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2374

Bisenić A, Tomić S, Bekić M, Pavlović L, Dinić M, Terzić- Vidojević A, Radojević D, Soković Bajić S, Mitrović H, Jakovljević S, Stevanović D, Golić N, Đokić J. SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2374 .

Bisenić, Aleksandar, Tomić, Sergej, Bekić, Marina, Pavlović, Luka, Dinić, Miroslav, Terzić- Vidojević, Amarela, Radojević, Dušan, Soković Bajić, Svetlana, Mitrović, Hristina, Jakovljević, Stefan, Stevanović, Dušan, Golić, Nataša, Đokić, Jelena, "SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):116-116,
https://hdl.handle.net/21.15107/rcub_imagine_2374 .

TY  - CONF
AU  - Dinić, Miroslav
AU  - L. Burgess, Jamie
AU  - Lukić, Jovanka
AU  - Catanuto, Paola
AU  - Radojević, Dušan
AU  - Marjanović, Jelena
AU  - Verpile, Rebecca
AU  - R. Thaller, Seth
AU  - Gonzalez, Tammy
AU  - Golić, Nataša
AU  - Tomić- Canić, Marjana
AU  - Strahinić, Ivana
AU  - Pastar, Irena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2378
AB  - Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING
EP  - 133
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2378
ER  - 

@conference{
author = "Dinić, Miroslav and L. Burgess, Jamie and Lukić, Jovanka and Catanuto, Paola and Radojević, Dušan and Marjanović, Jelena and Verpile, Rebecca and R. Thaller, Seth and Gonzalez, Tammy and Golić, Nataša and Tomić- Canić, Marjana and Strahinić, Ivana and Pastar, Irena",
year = "2024",
abstract = "Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2378"
}

Dinić, M., L. Burgess, J., Lukić, J., Catanuto, P., Radojević, D., Marjanović, J., Verpile, R., R. Thaller, S., Gonzalez, T., Golić, N., Tomić- Canić, M., Strahinić, I.,& Pastar, I.. (2024). HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378

Dinić M, L. Burgess J, Lukić J, Catanuto P, Radojević D, Marjanović J, Verpile R, R. Thaller S, Gonzalez T, Golić N, Tomić- Canić M, Strahinić I, Pastar I. HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

Dinić, Miroslav, L. Burgess, Jamie, Lukić, Jovanka, Catanuto, Paola, Radojević, Dušan, Marjanović, Jelena, Verpile, Rebecca, R. Thaller, Seth, Gonzalez, Tammy, Golić, Nataša, Tomić- Canić, Marjana, Strahinić, Ivana, Pastar, Irena, "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):133-133,
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

TY  - CONF
AU  - Đokić, Lidija
AU  - Rokić, Miloš
AU  - Šenerović, Lidija
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2376
AB  - The rapid emergence and spread of multidrug-
resistant pathogens present a global
healthcare challenge. One common cause of
resistance and/or tolerance to antibiotics is
biofilms, a complex communities of bacteria
embedded in a self-produced matrix. Biofilm
formation and maturation are regulated by
quorum sensing, a cell density-dependent communication
system that relies on the synthesis,
diffusion, and detection of small signaling molecules
- autoinducers (AIs). Quorum quenching
(QQ) enzymes that cut Ais emerged as a promising
strategy for persistent bacterial infections.
However, a significant drawback for the use of
QQ enzymes as therapeutics is their poor stability
and efficacy in vivo. Since one of the major
health issues linked to biofilm development is
persistent wound infections, our goal was to
improve enzyme properties by immobilizing it
on a natural biopolymer to make it suitable for
use as a wound dressing. The best candidate for immobilization was YtnP lactonase from Bacillus
paralicheniformis ZP1, as in concentrations
higher than 25 μg/mL it improved the survival of
Pseudomonas aeruginosa PAO1-infected zebrafish,
rescuing 80% of embryos. When combined
with tobramycin or gentamicin, the survival
rate of zebrafish embryos increased to 100%.
Purified YtnP lactonase at a concentration of 1
mg was immobilized on 10 mg of polymer disks
by crosslinking with glutaraldehyde. Specific
modifications of the polymer were also made to
eliminate the use of glutaraldehyde, which is a
skin irritant. In in vivo experiments on a murine
chronic wound model, immobilized enzyme
inhibited biofilm development, cleared already
formed biofilms, and overall improved wound
healing. These results provide a foundation for
the development of advanced wound dressings
that will prevent infection development in
wounds and enable proper therapy for infected
chronic wounds.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS
EP  - 126
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2376
ER  - 

@conference{
author = "Đokić, Lidija and Rokić, Miloš and Šenerović, Lidija",
year = "2024",
abstract = "The rapid emergence and spread of multidrug-
resistant pathogens present a global
healthcare challenge. One common cause of
resistance and/or tolerance to antibiotics is
biofilms, a complex communities of bacteria
embedded in a self-produced matrix. Biofilm
formation and maturation are regulated by
quorum sensing, a cell density-dependent communication
system that relies on the synthesis,
diffusion, and detection of small signaling molecules
- autoinducers (AIs). Quorum quenching
(QQ) enzymes that cut Ais emerged as a promising
strategy for persistent bacterial infections.
However, a significant drawback for the use of
QQ enzymes as therapeutics is their poor stability
and efficacy in vivo. Since one of the major
health issues linked to biofilm development is
persistent wound infections, our goal was to
improve enzyme properties by immobilizing it
on a natural biopolymer to make it suitable for
use as a wound dressing. The best candidate for immobilization was YtnP lactonase from Bacillus
paralicheniformis ZP1, as in concentrations
higher than 25 μg/mL it improved the survival of
Pseudomonas aeruginosa PAO1-infected zebrafish,
rescuing 80% of embryos. When combined
with tobramycin or gentamicin, the survival
rate of zebrafish embryos increased to 100%.
Purified YtnP lactonase at a concentration of 1
mg was immobilized on 10 mg of polymer disks
by crosslinking with glutaraldehyde. Specific
modifications of the polymer were also made to
eliminate the use of glutaraldehyde, which is a
skin irritant. In in vivo experiments on a murine
chronic wound model, immobilized enzyme
inhibited biofilm development, cleared already
formed biofilms, and overall improved wound
healing. These results provide a foundation for
the development of advanced wound dressings
that will prevent infection development in
wounds and enable proper therapy for infected
chronic wounds.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS",
pages = "126-126",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2376"
}

Đokić, L., Rokić, M.,& Šenerović, L.. (2024). NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 126-126.
https://hdl.handle.net/21.15107/rcub_imagine_2376

Đokić L, Rokić M, Šenerović L. NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:126-126.
https://hdl.handle.net/21.15107/rcub_imagine_2376 .

Đokić, Lidija, Rokić, Miloš, Šenerović, Lidija, "NEW APPROACHES IN THE TREATMENT OF CHRONIC BACTERIAL INFECTIONS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):126-126,
https://hdl.handle.net/21.15107/rcub_imagine_2376 .