Anti-encephalitogenic effects of cucumber leaf extract
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Authors
Jevtić, BojanĐedović, Neda
Stanisavljević, Suzana
Gašić, Uroš
Misić, Danijela
Despotović, Jovana
Samardžić, Jelena
Miljković, Djordje
Timotijević, Gordana
Article (Published version)
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Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further inves...tigation on its anti-encephalitogenic therapeutic properties.
Keywords:
T cell / Macrophage / Experimental autoimmune encephalomyelitis / Cucurbitacin / Cucumber / AutoimmunitySource:
Journal of Functional Foods, 2017, 37, 249-262Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
- Complex diseases as a model system for phenotype modulation- structural and functional analysis of molecular biomarkers (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.1016/j.jff.2017.07.060
ISSN: 1756-4646
WoS: 000412863400025
Scopus: 2-s2.0-85026788295
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jevtić, Bojan AU - Đedović, Neda AU - Stanisavljević, Suzana AU - Gašić, Uroš AU - Misić, Danijela AU - Despotović, Jovana AU - Samardžić, Jelena AU - Miljković, Djordje AU - Timotijević, Gordana PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1000 AB - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Functional Foods T1 - Anti-encephalitogenic effects of cucumber leaf extract EP - 262 SP - 249 VL - 37 DO - 10.1016/j.jff.2017.07.060 ER -
@article{ author = "Jevtić, Bojan and Đedović, Neda and Stanisavljević, Suzana and Gašić, Uroš and Misić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Djordje and Timotijević, Gordana", year = "2017", abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Functional Foods", title = "Anti-encephalitogenic effects of cucumber leaf extract", pages = "262-249", volume = "37", doi = "10.1016/j.jff.2017.07.060" }
Jevtić, B., Đedović, N., Stanisavljević, S., Gašić, U., Misić, D., Despotović, J., Samardžić, J., Miljković, D.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods Elsevier Science Bv, Amsterdam., 37, 249-262. https://doi.org/10.1016/j.jff.2017.07.060
Jevtić B, Đedović N, Stanisavljević S, Gašić U, Misić D, Despotović J, Samardžić J, Miljković D, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262. doi:10.1016/j.jff.2017.07.060 .
Jevtić, Bojan, Đedović, Neda, Stanisavljević, Suzana, Gašić, Uroš, Misić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Djordje, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262, https://doi.org/10.1016/j.jff.2017.07.060 . .