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Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4
dc.creator | Lozo, Jelena | |
dc.creator | Mirković, Nemanja | |
dc.creator | O'Connor, Paula M. | |
dc.creator | Malešević, Milka | |
dc.creator | Miljković, Marija | |
dc.creator | Polović, Natalija | |
dc.creator | Jovčić, Branko | |
dc.creator | Cotter, Paul D. | |
dc.creator | Kojić, Milan | |
dc.date.accessioned | 2022-11-15T14:47:50Z | |
dc.date.available | 2022-11-15T14:47:50Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0099-2240 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/1001 | |
dc.description.abstract | Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers. | en |
dc.publisher | Amer Soc Microbiology, Washington | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS// | |
dc.rights | restrictedAccess | |
dc.source | Applied and Environmental Microbiology | |
dc.subject | lactolisterin BU | en |
dc.subject | bacteriocin | en |
dc.subject | antilisterial activity | en |
dc.title | Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4 | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.issue | 21 | |
dc.citation.other | 83(21) | |
dc.citation.rank | M21 | |
dc.citation.volume | 83 | |
dc.identifier.doi | 10.1128/AEM.01519-17 | |
dc.identifier.pmid | 28842543 | |
dc.identifier.scopus | 2-s2.0-85031687597 | |
dc.identifier.wos | 000413104500013 | |
dc.type.version | publishedVersion |