Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells
Autori
Topalović, VladankaKrstić, Aleksandar
Schwirtlich, Marija
Dolfini, Diletta
Mantovani, Roberto
Stevanović, Milena
Mojsin, Marija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 cells. We show that the promoter of the human SOX3 gene is extremely hypomethylated both in undifferentiated NT2/D1 cells and during the early phases of RA-induced neural differentiation. By employing chromatin immunopre-cipitation, we analyze several histone modifications across different regions of the SOX3 gene and their dynamics following initiation of differentiation. In the same timeframe we investigate profiles of selected histone marks on the promoters of human SOX1 and SOX2 genes. We demonstrate differences in histone signatures of SOX1, SOX2 and SOX3 genes. Considering the importance of... SOXB1 genes in the process of neural differentiation, the present study contributes to a better understanding of epigenetic mechanisms implicated in the regulation of pluripotency maintenance and commitment towards the neural lineage.
Izvor:
PLoS One, 2017, 12, 9Izdavač:
- Public Library Science, San Francisco
Finansiranje / projekti:
- Proučavanje signalnih puteva i epigenetičkih mehanizama uključenih u kontrolu ekspresije humanih SOX gena: dalje rasvetljavanje njihove uloge u određivanju sudbine i diferencijaciji ćelija (RS-MESTD-Basic Research (BR or ON)-173051)
DOI: 10.1371/journal.pone.0184099
ISSN: 1932-6203
PubMed: 28886103
WoS: 000410001100067
Scopus: 2-s2.0-85029383616
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Topalović, Vladanka AU - Krstić, Aleksandar AU - Schwirtlich, Marija AU - Dolfini, Diletta AU - Mantovani, Roberto AU - Stevanović, Milena AU - Mojsin, Marija PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1005 AB - Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 cells. We show that the promoter of the human SOX3 gene is extremely hypomethylated both in undifferentiated NT2/D1 cells and during the early phases of RA-induced neural differentiation. By employing chromatin immunopre-cipitation, we analyze several histone modifications across different regions of the SOX3 gene and their dynamics following initiation of differentiation. In the same timeframe we investigate profiles of selected histone marks on the promoters of human SOX1 and SOX2 genes. We demonstrate differences in histone signatures of SOX1, SOX2 and SOX3 genes. Considering the importance of SOXB1 genes in the process of neural differentiation, the present study contributes to a better understanding of epigenetic mechanisms implicated in the regulation of pluripotency maintenance and commitment towards the neural lineage. PB - Public Library Science, San Francisco T2 - PLoS One T1 - Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells IS - 9 VL - 12 DO - 10.1371/journal.pone.0184099 ER -
@article{ author = "Topalović, Vladanka and Krstić, Aleksandar and Schwirtlich, Marija and Dolfini, Diletta and Mantovani, Roberto and Stevanović, Milena and Mojsin, Marija", year = "2017", abstract = "Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 cells. We show that the promoter of the human SOX3 gene is extremely hypomethylated both in undifferentiated NT2/D1 cells and during the early phases of RA-induced neural differentiation. By employing chromatin immunopre-cipitation, we analyze several histone modifications across different regions of the SOX3 gene and their dynamics following initiation of differentiation. In the same timeframe we investigate profiles of selected histone marks on the promoters of human SOX1 and SOX2 genes. We demonstrate differences in histone signatures of SOX1, SOX2 and SOX3 genes. Considering the importance of SOXB1 genes in the process of neural differentiation, the present study contributes to a better understanding of epigenetic mechanisms implicated in the regulation of pluripotency maintenance and commitment towards the neural lineage.", publisher = "Public Library Science, San Francisco", journal = "PLoS One", title = "Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells", number = "9", volume = "12", doi = "10.1371/journal.pone.0184099" }
Topalović, V., Krstić, A., Schwirtlich, M., Dolfini, D., Mantovani, R., Stevanović, M.,& Mojsin, M.. (2017). Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. in PLoS One Public Library Science, San Francisco., 12(9). https://doi.org/10.1371/journal.pone.0184099
Topalović V, Krstić A, Schwirtlich M, Dolfini D, Mantovani R, Stevanović M, Mojsin M. Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. in PLoS One. 2017;12(9). doi:10.1371/journal.pone.0184099 .
Topalović, Vladanka, Krstić, Aleksandar, Schwirtlich, Marija, Dolfini, Diletta, Mantovani, Roberto, Stevanović, Milena, Mojsin, Marija, "Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells" in PLoS One, 12, no. 9 (2017), https://doi.org/10.1371/journal.pone.0184099 . .