Characterization of a helicase, dhel III, from Drosophila melanogaster embryonic nuclei
Само за регистроване кориснике
1997
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Nuclear extracts prepared from Drosophila preblastoderm embryos contain a nucleoside 5'-triphosphate-dependent helicase that unwinds circular or linear partial duplex DNA substrates and moves along the DNA in 3' to 5' direction. The helicase reaction is supported with both nucleoside and deoxynucleoside 5'-triphosphates and requires divalent cations. Optimum activity in vitro is achieved with dATP or ATP and with MgCl2. In glycerol density gradients, embryonic enzyme migrates as a single peak of around 90kDa and it is the most prominent DNA-unwinding activity detectable in nuclear extracts prepared from embryos 0-2 hours after egg laying. In embryos collected 2-4 hours after egg laying this DNA unwinding activity increases and then gradually decreases in embryos collected 4-8 and 8-16 hours after egg laying.
Кључне речи:
NTP-dependent DNA-helicase / embryonic nuclei / DrosophilaИзвор:
Biochemistry and Molecular Biology International, 1997, 43, 4, 723-731Издавач:
- Taylor and Francis Inc.
DOI: 10.1080/15216549700204531
ISSN: 1039-9712
PubMed: 9385432
WoS: 000073191200004
Scopus: 2-s2.0-0031394971
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ristić, D AU - Todorović, V AU - Kojić, Snežana AU - Stefanović, D PY - 1997 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/103 AB - Nuclear extracts prepared from Drosophila preblastoderm embryos contain a nucleoside 5'-triphosphate-dependent helicase that unwinds circular or linear partial duplex DNA substrates and moves along the DNA in 3' to 5' direction. The helicase reaction is supported with both nucleoside and deoxynucleoside 5'-triphosphates and requires divalent cations. Optimum activity in vitro is achieved with dATP or ATP and with MgCl2. In glycerol density gradients, embryonic enzyme migrates as a single peak of around 90kDa and it is the most prominent DNA-unwinding activity detectable in nuclear extracts prepared from embryos 0-2 hours after egg laying. In embryos collected 2-4 hours after egg laying this DNA unwinding activity increases and then gradually decreases in embryos collected 4-8 and 8-16 hours after egg laying. PB - Taylor and Francis Inc. T2 - Biochemistry and Molecular Biology International T1 - Characterization of a helicase, dhel III, from Drosophila melanogaster embryonic nuclei EP - 731 IS - 4 SP - 723 VL - 43 DO - 10.1080/15216549700204531 ER -
@article{ author = "Ristić, D and Todorović, V and Kojić, Snežana and Stefanović, D", year = "1997", abstract = "Nuclear extracts prepared from Drosophila preblastoderm embryos contain a nucleoside 5'-triphosphate-dependent helicase that unwinds circular or linear partial duplex DNA substrates and moves along the DNA in 3' to 5' direction. The helicase reaction is supported with both nucleoside and deoxynucleoside 5'-triphosphates and requires divalent cations. Optimum activity in vitro is achieved with dATP or ATP and with MgCl2. In glycerol density gradients, embryonic enzyme migrates as a single peak of around 90kDa and it is the most prominent DNA-unwinding activity detectable in nuclear extracts prepared from embryos 0-2 hours after egg laying. In embryos collected 2-4 hours after egg laying this DNA unwinding activity increases and then gradually decreases in embryos collected 4-8 and 8-16 hours after egg laying.", publisher = "Taylor and Francis Inc.", journal = "Biochemistry and Molecular Biology International", title = "Characterization of a helicase, dhel III, from Drosophila melanogaster embryonic nuclei", pages = "731-723", number = "4", volume = "43", doi = "10.1080/15216549700204531" }
Ristić, D., Todorović, V., Kojić, S.,& Stefanović, D.. (1997). Characterization of a helicase, dhel III, from Drosophila melanogaster embryonic nuclei. in Biochemistry and Molecular Biology International Taylor and Francis Inc.., 43(4), 723-731. https://doi.org/10.1080/15216549700204531
Ristić D, Todorović V, Kojić S, Stefanović D. Characterization of a helicase, dhel III, from Drosophila melanogaster embryonic nuclei. in Biochemistry and Molecular Biology International. 1997;43(4):723-731. doi:10.1080/15216549700204531 .
Ristić, D, Todorović, V, Kojić, Snežana, Stefanović, D, "Characterization of a helicase, dhel III, from Drosophila melanogaster embryonic nuclei" in Biochemistry and Molecular Biology International, 43, no. 4 (1997):723-731, https://doi.org/10.1080/15216549700204531 . .