Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows
2017
Autori
Veljović, KatarinaDinić, Miroslav
Lukić, Jovanka
Mihajlović, Sanja
Tolinački, Maja
Živković, Milica
Begović, Jelena
Mrvaljević, Igor
Golić, Nataša
Terzić-Vidojević, Amarela
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA4...3 and BGHI14 (p lt 0.05), as well as probiotic combination (p lt 0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p lt 0.01) and BGRA43 (p lt 0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.
Ključne reči:
probiotic / pathogen exclusion / neonatal piglets / microbiota diversity / DGGEIzvor:
Frontiers in Microbiology, 2017, 8Izdavač:
- Frontiers Media Sa, Lausanne
Finansiranje / projekti:
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
- 451-03-2802/2013-16/148
DOI: 10.3389/fmicb.2017.02028
ISSN: 1664-302X
WoS: 000413411200001
Scopus: 2-s2.0-85031716146
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Veljović, Katarina AU - Dinić, Miroslav AU - Lukić, Jovanka AU - Mihajlović, Sanja AU - Tolinački, Maja AU - Živković, Milica AU - Begović, Jelena AU - Mrvaljević, Igor AU - Golić, Nataša AU - Terzić-Vidojević, Amarela PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1031 AB - The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p lt 0.05), as well as probiotic combination (p lt 0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p lt 0.01) and BGRA43 (p lt 0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows VL - 8 DO - 10.3389/fmicb.2017.02028 ER -
@article{ author = "Veljović, Katarina and Dinić, Miroslav and Lukić, Jovanka and Mihajlović, Sanja and Tolinački, Maja and Živković, Milica and Begović, Jelena and Mrvaljević, Igor and Golić, Nataša and Terzić-Vidojević, Amarela", year = "2017", abstract = "The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p lt 0.05), as well as probiotic combination (p lt 0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p lt 0.01) and BGRA43 (p lt 0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows", volume = "8", doi = "10.3389/fmicb.2017.02028" }
Veljović, K., Dinić, M., Lukić, J., Mihajlović, S., Tolinački, M., Živković, M., Begović, J., Mrvaljević, I., Golić, N.,& Terzić-Vidojević, A.. (2017). Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 8. https://doi.org/10.3389/fmicb.2017.02028
Veljović K, Dinić M, Lukić J, Mihajlović S, Tolinački M, Živković M, Begović J, Mrvaljević I, Golić N, Terzić-Vidojević A. Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology. 2017;8. doi:10.3389/fmicb.2017.02028 .
Veljović, Katarina, Dinić, Miroslav, Lukić, Jovanka, Mihajlović, Sanja, Tolinački, Maja, Živković, Milica, Begović, Jelena, Mrvaljević, Igor, Golić, Nataša, Terzić-Vidojević, Amarela, "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows" in Frontiers in Microbiology, 8 (2017), https://doi.org/10.3389/fmicb.2017.02028 . .