PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli
Апстракт
PspF bound to the psp enhancer activates E sigma(54) holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma(70)-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host factor (IHF) bound to a DNA site located between the psp core promoter and the PspF binding sites stimulates psp expression. We show here that wild-type IHF strongly retards DNA containing the psp promoter region. In vitro, PspF binding to the psp enhancer facilitates IHF binding, while IHF binding to the pspF-pspA-E promoter-regulatory region increases the efficacy of PspF binding to the upstream activating sequences (UASs). This is the first demonstration of co-operative binding of an activator and IHF in a sigma(54)-dependent system. In the absence of IHF, in vivo autoregulation of pspF transcription is lifted and, consequently, PspF production is increased, indicat...ing that IHF enhances PspF binding to the psp enhancer in vivo.
Извор:
Molecular Microbiology, 1997, 25, 3, 473-481Издавач:
- Wiley-Blackwell, Hoboken
DOI: 10.1046/j.1365-2958.1997.4791844.x
ISSN: 0950-382X
PubMed: 9302010
WoS: A1997XW00900005
Scopus: 2-s2.0-0030881602
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jovanović, Goran AU - Model, P PY - 1997 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/105 AB - PspF bound to the psp enhancer activates E sigma(54) holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma(70)-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host factor (IHF) bound to a DNA site located between the psp core promoter and the PspF binding sites stimulates psp expression. We show here that wild-type IHF strongly retards DNA containing the psp promoter region. In vitro, PspF binding to the psp enhancer facilitates IHF binding, while IHF binding to the pspF-pspA-E promoter-regulatory region increases the efficacy of PspF binding to the upstream activating sequences (UASs). This is the first demonstration of co-operative binding of an activator and IHF in a sigma(54)-dependent system. In the absence of IHF, in vivo autoregulation of pspF transcription is lifted and, consequently, PspF production is increased, indicating that IHF enhances PspF binding to the psp enhancer in vivo. PB - Wiley-Blackwell, Hoboken T2 - Molecular Microbiology T1 - PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli EP - 481 IS - 3 SP - 473 VL - 25 DO - 10.1046/j.1365-2958.1997.4791844.x ER -
@article{ author = "Jovanović, Goran and Model, P", year = "1997", abstract = "PspF bound to the psp enhancer activates E sigma(54) holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma(70)-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host factor (IHF) bound to a DNA site located between the psp core promoter and the PspF binding sites stimulates psp expression. We show here that wild-type IHF strongly retards DNA containing the psp promoter region. In vitro, PspF binding to the psp enhancer facilitates IHF binding, while IHF binding to the pspF-pspA-E promoter-regulatory region increases the efficacy of PspF binding to the upstream activating sequences (UASs). This is the first demonstration of co-operative binding of an activator and IHF in a sigma(54)-dependent system. In the absence of IHF, in vivo autoregulation of pspF transcription is lifted and, consequently, PspF production is increased, indicating that IHF enhances PspF binding to the psp enhancer in vivo.", publisher = "Wiley-Blackwell, Hoboken", journal = "Molecular Microbiology", title = "PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli", pages = "481-473", number = "3", volume = "25", doi = "10.1046/j.1365-2958.1997.4791844.x" }
Jovanović, G.,& Model, P.. (1997). PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli. in Molecular Microbiology Wiley-Blackwell, Hoboken., 25(3), 473-481. https://doi.org/10.1046/j.1365-2958.1997.4791844.x
Jovanović G, Model P. PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli. in Molecular Microbiology. 1997;25(3):473-481. doi:10.1046/j.1365-2958.1997.4791844.x .
Jovanović, Goran, Model, P, "PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli" in Molecular Microbiology, 25, no. 3 (1997):473-481, https://doi.org/10.1046/j.1365-2958.1997.4791844.x . .