Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3
Само за регистроване кориснике
1997
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
We describe the use of transcriptional fusions to the phage shock protein (psp) promoter. These fusions are expressed only when cells are infected by filamentous phage. In an application, the psp promoter was fused to the protein coding part of filamentous phage gene III (gIII). Protein III (pIII) is needed to complement mutant fl phage containing a deletion of gIII but its synthesis also renders cells resistant to infection. By inducing pIII production from psp-gIII only in the cells that are already infected with phage, it was possible to obtain plaques from phage in which gill had been completely deleted. gIII was deleted from two helper phages: R408 and VCSM13, which were then propagated on cells containing the psp-gIII fusion. These two phages were tested for use in a phage display method that requires generation of noninfectious, phagemid-containing virion-like particles. Both helpers worked, but R408d3 was superior to VCSM13d3, because it generated about 1800-times fewer backgro...und infectious particles.
Кључне речи:
psp operon / promoter fusions / phage display / gene III encoded proteinИзвор:
Gene, 1997, 198, 1-2, 99-103Издавач:
- Elsevier Science Bv, Amsterdam
DOI: 10.1016/S0378-1119(97)00298-9
ISSN: 0378-1119
PubMed: 9370269
WoS: A1997YA83400012
Scopus: 2-s2.0-0030667838
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Rakonjac, J AU - Jovanović, Goran AU - Model, P PY - 1997 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/106 AB - We describe the use of transcriptional fusions to the phage shock protein (psp) promoter. These fusions are expressed only when cells are infected by filamentous phage. In an application, the psp promoter was fused to the protein coding part of filamentous phage gene III (gIII). Protein III (pIII) is needed to complement mutant fl phage containing a deletion of gIII but its synthesis also renders cells resistant to infection. By inducing pIII production from psp-gIII only in the cells that are already infected with phage, it was possible to obtain plaques from phage in which gill had been completely deleted. gIII was deleted from two helper phages: R408 and VCSM13, which were then propagated on cells containing the psp-gIII fusion. These two phages were tested for use in a phage display method that requires generation of noninfectious, phagemid-containing virion-like particles. Both helpers worked, but R408d3 was superior to VCSM13d3, because it generated about 1800-times fewer background infectious particles. PB - Elsevier Science Bv, Amsterdam T2 - Gene T1 - Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3 EP - 103 IS - 1-2 SP - 99 VL - 198 DO - 10.1016/S0378-1119(97)00298-9 ER -
@article{ author = "Rakonjac, J and Jovanović, Goran and Model, P", year = "1997", abstract = "We describe the use of transcriptional fusions to the phage shock protein (psp) promoter. These fusions are expressed only when cells are infected by filamentous phage. In an application, the psp promoter was fused to the protein coding part of filamentous phage gene III (gIII). Protein III (pIII) is needed to complement mutant fl phage containing a deletion of gIII but its synthesis also renders cells resistant to infection. By inducing pIII production from psp-gIII only in the cells that are already infected with phage, it was possible to obtain plaques from phage in which gill had been completely deleted. gIII was deleted from two helper phages: R408 and VCSM13, which were then propagated on cells containing the psp-gIII fusion. These two phages were tested for use in a phage display method that requires generation of noninfectious, phagemid-containing virion-like particles. Both helpers worked, but R408d3 was superior to VCSM13d3, because it generated about 1800-times fewer background infectious particles.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Gene", title = "Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3", pages = "103-99", number = "1-2", volume = "198", doi = "10.1016/S0378-1119(97)00298-9" }
Rakonjac, J., Jovanović, G.,& Model, P.. (1997). Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3. in Gene Elsevier Science Bv, Amsterdam., 198(1-2), 99-103. https://doi.org/10.1016/S0378-1119(97)00298-9
Rakonjac J, Jovanović G, Model P. Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3. in Gene. 1997;198(1-2):99-103. doi:10.1016/S0378-1119(97)00298-9 .
Rakonjac, J, Jovanović, Goran, Model, P, "Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3" in Gene, 198, no. 1-2 (1997):99-103, https://doi.org/10.1016/S0378-1119(97)00298-9 . .