Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells
Апстракт
Transcription factors OCT4 and NANOG are main constituents of a functional network that controls proliferation and pluripotency maintenance of stem cells as well as early lineage decisions. We investigated expression profiles of OCT4 and NANOG during the early phases of neural differentiation using NT2/D1 cells induced by retinoic acid as an in vitro model system of human neurogenesis. We demonstrated decrease in OCT4 and NANOG mRNA and protein levels following exposure to retinoic acid. Next, by employing chromatin immunoprecipitation, we investigated profiles of selected H3 and H2B histone marks deposited on the promoters of the OCT4 and NANOG genes. We found decline in H3K4me3, H2BK5ac, and H2BK120ac on both promoters, which paralleled the decrease in OCT4 and NANOG expression. Moreover, we found that the H2BK16ac mark is differentially enriched on these two promoters, pointing to differences in epigenetic regulation of OCT4 and NANOG gene expression. Finally, based on our data, we ...suggest that the early response of pluripotency genes OCT4 and NANOG to the differentiation-inducing stimuli is mediated by dynamic changes in chromatin marks, while DNA methylation is acquired in the later stages of neurogenesis.
Кључне речи:
OCT4 / NT2/D1 cell line / NANOG / histone modificationsИзвор:
Biochemistry-Moscow, 2017, 82, 6, 715-722Издавач:
- Maik Nauka/Interperiodica/Springer, New York
Финансирање / пројекти:
- Проучавање сигналних путева и епигенетичких механизама укључених у контролу експресије хуманих SOX гена: даље расветљавање њихове улоге у одређивању судбине и диференцијацији ћелија (RS-MESTD-Basic Research (BR or ON)-173051)
DOI: 10.1134/S0006297917060086
ISSN: 0006-2979
PubMed: 28601081
WoS: 000403456200008
Scopus: 2-s2.0-85020708521
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Topalović, Vladanka AU - Schwirtlich, Marija AU - Stevanović, Milena AU - Mojsin, Marija PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1077 AB - Transcription factors OCT4 and NANOG are main constituents of a functional network that controls proliferation and pluripotency maintenance of stem cells as well as early lineage decisions. We investigated expression profiles of OCT4 and NANOG during the early phases of neural differentiation using NT2/D1 cells induced by retinoic acid as an in vitro model system of human neurogenesis. We demonstrated decrease in OCT4 and NANOG mRNA and protein levels following exposure to retinoic acid. Next, by employing chromatin immunoprecipitation, we investigated profiles of selected H3 and H2B histone marks deposited on the promoters of the OCT4 and NANOG genes. We found decline in H3K4me3, H2BK5ac, and H2BK120ac on both promoters, which paralleled the decrease in OCT4 and NANOG expression. Moreover, we found that the H2BK16ac mark is differentially enriched on these two promoters, pointing to differences in epigenetic regulation of OCT4 and NANOG gene expression. Finally, based on our data, we suggest that the early response of pluripotency genes OCT4 and NANOG to the differentiation-inducing stimuli is mediated by dynamic changes in chromatin marks, while DNA methylation is acquired in the later stages of neurogenesis. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Biochemistry-Moscow T1 - Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells EP - 722 IS - 6 SP - 715 VL - 82 DO - 10.1134/S0006297917060086 ER -
@article{ author = "Topalović, Vladanka and Schwirtlich, Marija and Stevanović, Milena and Mojsin, Marija", year = "2017", abstract = "Transcription factors OCT4 and NANOG are main constituents of a functional network that controls proliferation and pluripotency maintenance of stem cells as well as early lineage decisions. We investigated expression profiles of OCT4 and NANOG during the early phases of neural differentiation using NT2/D1 cells induced by retinoic acid as an in vitro model system of human neurogenesis. We demonstrated decrease in OCT4 and NANOG mRNA and protein levels following exposure to retinoic acid. Next, by employing chromatin immunoprecipitation, we investigated profiles of selected H3 and H2B histone marks deposited on the promoters of the OCT4 and NANOG genes. We found decline in H3K4me3, H2BK5ac, and H2BK120ac on both promoters, which paralleled the decrease in OCT4 and NANOG expression. Moreover, we found that the H2BK16ac mark is differentially enriched on these two promoters, pointing to differences in epigenetic regulation of OCT4 and NANOG gene expression. Finally, based on our data, we suggest that the early response of pluripotency genes OCT4 and NANOG to the differentiation-inducing stimuli is mediated by dynamic changes in chromatin marks, while DNA methylation is acquired in the later stages of neurogenesis.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Biochemistry-Moscow", title = "Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells", pages = "722-715", number = "6", volume = "82", doi = "10.1134/S0006297917060086" }
Topalović, V., Schwirtlich, M., Stevanović, M.,& Mojsin, M.. (2017). Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells. in Biochemistry-Moscow Maik Nauka/Interperiodica/Springer, New York., 82(6), 715-722. https://doi.org/10.1134/S0006297917060086
Topalović V, Schwirtlich M, Stevanović M, Mojsin M. Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells. in Biochemistry-Moscow. 2017;82(6):715-722. doi:10.1134/S0006297917060086 .
Topalović, Vladanka, Schwirtlich, Marija, Stevanović, Milena, Mojsin, Marija, "Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells" in Biochemistry-Moscow, 82, no. 6 (2017):715-722, https://doi.org/10.1134/S0006297917060086 . .