Complementary approaches for the evaluation of biocompatibility of Y-90-labeled superparamagnetic citric acid (Fe,Er)(3)O-4 coated nanoparticles
Samo za registrovane korisnike
2017
Autori
Antić, BratislavBosković, Marko
Nikodinović-Runić, Jasmina
Ming, Yue
Zhang, Hongguo
Bozin, Emil S.
Janković, Drina
Spasojević, Vojislav
Vranjes-Djurić, Sanja
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Magnetic nanoparticles (MNPs) are of immense interest for diagnostic and therapeutic applications in medicine. Design and development of new iron oxide-based MNPs for such applications is of rather limited breadth without reliable and sensitive methods to determine their levels in body tissues. Commonly used methods, such as ICP, are quite problematic, due to the inability to decipher the origin of the detected iron, i.e. whether it originates from the MNPs or endogenous from tissues and bodily fluids. One of the approaches to overcome this problem and to increase reliability of tracing MNPs is to partially substitute iron ions in the MNPs with Er. Here, we report on the development of citric add coated (Fe,Er)(3)O-4 nanopartides and characterization of their physico-chemical and biological properties by utilization of various complementary approaches. The synthesized MNPs had a narrow (6-7 nm) size distribution, as consistently seen in atomic pair distribution function, transmission e...lectron microscopy, and DC magnetization measurements. The particles were found to be superparamagnetic, with a pronounced maximum in measured zero-field cooled magnetization at around 90 K. Reduction in saturation magnetization due to incorporation of 1.7% Er3+ into the Fe3O4 matrix was clearly observed. From the biological standpoint, citric acid coated (Fe,Er)(3)O-4 NPs were found to induce low toxicity both in human cell fibroblasts and in zebrafish (Danio rerio) embryos. Biodistribution pattern of the MNPs after intravenous administration in healthy Wistar rats was followed by the radiotracer method, revealing that Y-90-labeled MNPs were predominantly found in liver (7533% ID), followed by lungs (16.70% ID) and spleen (2.83% ID). Quantitative agreement with these observations was obtained by ICP-MS elemental analysis using Er as the detected tracer. Based on the favorable physical, chemical and biological characteristics, citric add coated (Fe,Er)(3)O-4 MNPs could be further considered for the potential application as a diagnostic and/or therapeutic agent. This work also demonstrates that combined application of these techniques is a promising tool for studies of pharmacokinetics of the new MNPs in complex biological systems.
Ključne reči:
Radiolabeling / MNP / Magnetic nanopartide / Embryotoxicity / BiodistributionIzvor:
Materials Science & Engineering C-Materials For Biological Applications, 2017, 75, 157-164Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Magnetni i radionuklidima obeleženi nanostrukturni materijali za primene u medicini (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45015)
- International S&T Cooperation Program of China [2015DFG52020]
- Izučavanje mikrobiološkog diverziteta i karakterizacija korisnih sredinskih mikroorganizama (RS-MESTD-Basic Research (BR or ON)-173048)
- US DOE, Office of Science, Office of Basic Energy Sciences (DOE-BES) [DE-SC00112704]
Napomena:
- Peer-reviewed manuscript: https://imagine.imgge.bg.ac.rs/handle/123456789/1763
- Supplementary data: https://imagine.imgge.bg.ac.rs/handle/123456789/1764
Povezane informacije:
- Povezani sadržaj
https://imagine.imgge.bg.ac.rs/handle/123456789/1764 - Povezani sadržaj
https://imagine.imgge.bg.ac.rs/handle/123456789/1763
DOI: 10.1016/j.msec.2017.02.023
ISSN: 0928-4931
PubMed: 28415449
WoS: 000400720800019
Scopus: 2-s2.0-85013041507
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Antić, Bratislav AU - Bosković, Marko AU - Nikodinović-Runić, Jasmina AU - Ming, Yue AU - Zhang, Hongguo AU - Bozin, Emil S. AU - Janković, Drina AU - Spasojević, Vojislav AU - Vranjes-Djurić, Sanja PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1082 AB - Magnetic nanoparticles (MNPs) are of immense interest for diagnostic and therapeutic applications in medicine. Design and development of new iron oxide-based MNPs for such applications is of rather limited breadth without reliable and sensitive methods to determine their levels in body tissues. Commonly used methods, such as ICP, are quite problematic, due to the inability to decipher the origin of the detected iron, i.e. whether it originates from the MNPs or endogenous from tissues and bodily fluids. One of the approaches to overcome this problem and to increase reliability of tracing MNPs is to partially substitute iron ions in the MNPs with Er. Here, we report on the development of citric add coated (Fe,Er)(3)O-4 nanopartides and characterization of their physico-chemical and biological properties by utilization of various complementary approaches. The synthesized MNPs had a narrow (6-7 nm) size distribution, as consistently seen in atomic pair distribution function, transmission electron microscopy, and DC magnetization measurements. The particles were found to be superparamagnetic, with a pronounced maximum in measured zero-field cooled magnetization at around 90 K. Reduction in saturation magnetization due to incorporation of 1.7% Er3+ into the Fe3O4 matrix was clearly observed. From the biological standpoint, citric acid coated (Fe,Er)(3)O-4 NPs were found to induce low toxicity both in human cell fibroblasts and in zebrafish (Danio rerio) embryos. Biodistribution pattern of the MNPs after intravenous administration in healthy Wistar rats was followed by the radiotracer method, revealing that Y-90-labeled MNPs were predominantly found in liver (7533% ID), followed by lungs (16.70% ID) and spleen (2.83% ID). Quantitative agreement with these observations was obtained by ICP-MS elemental analysis using Er as the detected tracer. Based on the favorable physical, chemical and biological characteristics, citric add coated (Fe,Er)(3)O-4 MNPs could be further considered for the potential application as a diagnostic and/or therapeutic agent. This work also demonstrates that combined application of these techniques is a promising tool for studies of pharmacokinetics of the new MNPs in complex biological systems. PB - Elsevier, Amsterdam T2 - Materials Science & Engineering C-Materials For Biological Applications T1 - Complementary approaches for the evaluation of biocompatibility of Y-90-labeled superparamagnetic citric acid (Fe,Er)(3)O-4 coated nanoparticles EP - 164 SP - 157 VL - 75 DO - 10.1016/j.msec.2017.02.023 ER -
@article{ author = "Antić, Bratislav and Bosković, Marko and Nikodinović-Runić, Jasmina and Ming, Yue and Zhang, Hongguo and Bozin, Emil S. and Janković, Drina and Spasojević, Vojislav and Vranjes-Djurić, Sanja", year = "2017", abstract = "Magnetic nanoparticles (MNPs) are of immense interest for diagnostic and therapeutic applications in medicine. Design and development of new iron oxide-based MNPs for such applications is of rather limited breadth without reliable and sensitive methods to determine their levels in body tissues. Commonly used methods, such as ICP, are quite problematic, due to the inability to decipher the origin of the detected iron, i.e. whether it originates from the MNPs or endogenous from tissues and bodily fluids. One of the approaches to overcome this problem and to increase reliability of tracing MNPs is to partially substitute iron ions in the MNPs with Er. Here, we report on the development of citric add coated (Fe,Er)(3)O-4 nanopartides and characterization of their physico-chemical and biological properties by utilization of various complementary approaches. The synthesized MNPs had a narrow (6-7 nm) size distribution, as consistently seen in atomic pair distribution function, transmission electron microscopy, and DC magnetization measurements. The particles were found to be superparamagnetic, with a pronounced maximum in measured zero-field cooled magnetization at around 90 K. Reduction in saturation magnetization due to incorporation of 1.7% Er3+ into the Fe3O4 matrix was clearly observed. From the biological standpoint, citric acid coated (Fe,Er)(3)O-4 NPs were found to induce low toxicity both in human cell fibroblasts and in zebrafish (Danio rerio) embryos. Biodistribution pattern of the MNPs after intravenous administration in healthy Wistar rats was followed by the radiotracer method, revealing that Y-90-labeled MNPs were predominantly found in liver (7533% ID), followed by lungs (16.70% ID) and spleen (2.83% ID). Quantitative agreement with these observations was obtained by ICP-MS elemental analysis using Er as the detected tracer. Based on the favorable physical, chemical and biological characteristics, citric add coated (Fe,Er)(3)O-4 MNPs could be further considered for the potential application as a diagnostic and/or therapeutic agent. This work also demonstrates that combined application of these techniques is a promising tool for studies of pharmacokinetics of the new MNPs in complex biological systems.", publisher = "Elsevier, Amsterdam", journal = "Materials Science & Engineering C-Materials For Biological Applications", title = "Complementary approaches for the evaluation of biocompatibility of Y-90-labeled superparamagnetic citric acid (Fe,Er)(3)O-4 coated nanoparticles", pages = "164-157", volume = "75", doi = "10.1016/j.msec.2017.02.023" }
Antić, B., Bosković, M., Nikodinović-Runić, J., Ming, Y., Zhang, H., Bozin, E. S., Janković, D., Spasojević, V.,& Vranjes-Djurić, S.. (2017). Complementary approaches for the evaluation of biocompatibility of Y-90-labeled superparamagnetic citric acid (Fe,Er)(3)O-4 coated nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications Elsevier, Amsterdam., 75, 157-164. https://doi.org/10.1016/j.msec.2017.02.023
Antić B, Bosković M, Nikodinović-Runić J, Ming Y, Zhang H, Bozin ES, Janković D, Spasojević V, Vranjes-Djurić S. Complementary approaches for the evaluation of biocompatibility of Y-90-labeled superparamagnetic citric acid (Fe,Er)(3)O-4 coated nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications. 2017;75:157-164. doi:10.1016/j.msec.2017.02.023 .
Antić, Bratislav, Bosković, Marko, Nikodinović-Runić, Jasmina, Ming, Yue, Zhang, Hongguo, Bozin, Emil S., Janković, Drina, Spasojević, Vojislav, Vranjes-Djurić, Sanja, "Complementary approaches for the evaluation of biocompatibility of Y-90-labeled superparamagnetic citric acid (Fe,Er)(3)O-4 coated nanoparticles" in Materials Science & Engineering C-Materials For Biological Applications, 75 (2017):157-164, https://doi.org/10.1016/j.msec.2017.02.023 . .