Agrobacterium rhizogenes-mediated transformation and plant regeneration of four Gentiana species
Samo za registrovane korisnike
1997
Članak u časopisu (Objavljena verzija)
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Shoots of micropropagated Gentiana acaulis, G. cruciata, G. lutea, and G. purpurea were inoculated with suspensions of Agrobacterium rhizogenes cells, strains ATCC 15834 or A4M70GUS. Adventitious roots appeared at the sites of inoculation in all 4 species. Root tips were excised and cultured on growth regulator-free media for 2-6 years. They exhibited very high branching and plagiotropism. Spontaneous bud initiation occurred in roots of G. cruciata. Roots of G. lutea, G. acaulis and G. purpurea were cultured on media with high kinetin concentration, which induced the formation of friable callus tissues. Only in G. purpurea were these calluses organogenic. Regenerated shoots of G. cruciata and G. purpurea gave rise to plants, that displayed the typical phenotypes of A. rhizogenes-transformed plants: short internodes and rolled leaves. In the roots of G. acaulis and G. cruciata, transformed with A. rhizogenes A4M70GUS, a positive reaction with X-gluc indicated the activity of beta-glucur...onidase. The DNA extracted from hairy roots and from the roots of transgenic plants hybridized with the appropriate genomic probes in Southern blotting. This is taken as evidence of the stable genetic transformation in the 4 Gentiana species.
Ključne reči:
plant regeneration / hairy roots / G-lutea L. / Gentiana acaulis L. / G-cruciata L. / G purpurea L. / Agrobacterium rhizogenesIzvor:
Plant Cell Tissue and Organ Culture, 1997, 50, 1, 1-6Izdavač:
- Springer, Dordrecht
DOI: 10.1023/A:1005880802231
ISSN: 0167-6857
WoS: A1997YH32900001
Scopus: 2-s2.0-0030668013
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Momcilović, I AU - Grubisić, D AU - Kojić, Milan AU - Nesković, M PY - 1997 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/108 AB - Shoots of micropropagated Gentiana acaulis, G. cruciata, G. lutea, and G. purpurea were inoculated with suspensions of Agrobacterium rhizogenes cells, strains ATCC 15834 or A4M70GUS. Adventitious roots appeared at the sites of inoculation in all 4 species. Root tips were excised and cultured on growth regulator-free media for 2-6 years. They exhibited very high branching and plagiotropism. Spontaneous bud initiation occurred in roots of G. cruciata. Roots of G. lutea, G. acaulis and G. purpurea were cultured on media with high kinetin concentration, which induced the formation of friable callus tissues. Only in G. purpurea were these calluses organogenic. Regenerated shoots of G. cruciata and G. purpurea gave rise to plants, that displayed the typical phenotypes of A. rhizogenes-transformed plants: short internodes and rolled leaves. In the roots of G. acaulis and G. cruciata, transformed with A. rhizogenes A4M70GUS, a positive reaction with X-gluc indicated the activity of beta-glucuronidase. The DNA extracted from hairy roots and from the roots of transgenic plants hybridized with the appropriate genomic probes in Southern blotting. This is taken as evidence of the stable genetic transformation in the 4 Gentiana species. PB - Springer, Dordrecht T2 - Plant Cell Tissue and Organ Culture T1 - Agrobacterium rhizogenes-mediated transformation and plant regeneration of four Gentiana species EP - 6 IS - 1 SP - 1 VL - 50 DO - 10.1023/A:1005880802231 ER -
@article{ author = "Momcilović, I and Grubisić, D and Kojić, Milan and Nesković, M", year = "1997", abstract = "Shoots of micropropagated Gentiana acaulis, G. cruciata, G. lutea, and G. purpurea were inoculated with suspensions of Agrobacterium rhizogenes cells, strains ATCC 15834 or A4M70GUS. Adventitious roots appeared at the sites of inoculation in all 4 species. Root tips were excised and cultured on growth regulator-free media for 2-6 years. They exhibited very high branching and plagiotropism. Spontaneous bud initiation occurred in roots of G. cruciata. Roots of G. lutea, G. acaulis and G. purpurea were cultured on media with high kinetin concentration, which induced the formation of friable callus tissues. Only in G. purpurea were these calluses organogenic. Regenerated shoots of G. cruciata and G. purpurea gave rise to plants, that displayed the typical phenotypes of A. rhizogenes-transformed plants: short internodes and rolled leaves. In the roots of G. acaulis and G. cruciata, transformed with A. rhizogenes A4M70GUS, a positive reaction with X-gluc indicated the activity of beta-glucuronidase. The DNA extracted from hairy roots and from the roots of transgenic plants hybridized with the appropriate genomic probes in Southern blotting. This is taken as evidence of the stable genetic transformation in the 4 Gentiana species.", publisher = "Springer, Dordrecht", journal = "Plant Cell Tissue and Organ Culture", title = "Agrobacterium rhizogenes-mediated transformation and plant regeneration of four Gentiana species", pages = "6-1", number = "1", volume = "50", doi = "10.1023/A:1005880802231" }
Momcilović, I., Grubisić, D., Kojić, M.,& Nesković, M.. (1997). Agrobacterium rhizogenes-mediated transformation and plant regeneration of four Gentiana species. in Plant Cell Tissue and Organ Culture Springer, Dordrecht., 50(1), 1-6. https://doi.org/10.1023/A:1005880802231
Momcilović I, Grubisić D, Kojić M, Nesković M. Agrobacterium rhizogenes-mediated transformation and plant regeneration of four Gentiana species. in Plant Cell Tissue and Organ Culture. 1997;50(1):1-6. doi:10.1023/A:1005880802231 .
Momcilović, I, Grubisić, D, Kojić, Milan, Nesković, M, "Agrobacterium rhizogenes-mediated transformation and plant regeneration of four Gentiana species" in Plant Cell Tissue and Organ Culture, 50, no. 1 (1997):1-6, https://doi.org/10.1023/A:1005880802231 . .