The RIB element in the goaG-pspF intergenic region of Escherichia coli
Апстракт
The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined, The pspF gene encodes a sigma(54) transcriptional activator of the phage shock protein (psp) operon (pspA to pspE), Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL, and goaG, convergently oriented with respect to pspF, These two ORFs, together with the adjacent aldH gene, may constitute a novel operon (aldH-ordL-goaG), The goaG-pspF intergenic region contains a complex extragenic mosaic element, RIB, The structure of this RIB element, which belongs to the BIME-1 family, is Y(REP1) gt 16 lt Z(1)(REP2), where Y and Z(1) are palindromic units and the central 16 bases contain an L motif with an ihf consensus sequence. DNA fragments containing the L motif of the psp RIB element effectively bind integration host factor (IHF), while the Y palindromic unit (REP1) of the same RIB element binds DNA gyrase weakly. Computer prediction of the pspF mRNA secondary structu...re suggested that the transcribed stem-loop structures formed by the 3'-flanking region of the pspF transcript containing the RIB element can stabilize and protect pspF mRNA, Analysis of pspF steady-state mRNA levels showed that transcripts,vith an intact RIB element are much more abundant than those truncated at the 3' end by deletion of either the entire RIB element or a single Z(1) sequence (REP2), Thus, the pspF 3'-flanking region containing the RIB element has an important role in the stabilization of the pspF transcript.
Извор:
Journal of Bacteriology, 1997, 179, 10, 3095-3102Издавач:
- Amer Soc Microbiology, Washington
DOI: 10.1128/jb.179.10.3095-3102.1997
ISSN: 0021-9193
PubMed: 9150200
WoS: A1997WY11600002
Scopus: 2-s2.0-0030973448
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jovanović, Goran AU - Model, P PY - 1997 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/109 AB - The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined, The pspF gene encodes a sigma(54) transcriptional activator of the phage shock protein (psp) operon (pspA to pspE), Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL, and goaG, convergently oriented with respect to pspF, These two ORFs, together with the adjacent aldH gene, may constitute a novel operon (aldH-ordL-goaG), The goaG-pspF intergenic region contains a complex extragenic mosaic element, RIB, The structure of this RIB element, which belongs to the BIME-1 family, is Y(REP1) gt 16 lt Z(1)(REP2), where Y and Z(1) are palindromic units and the central 16 bases contain an L motif with an ihf consensus sequence. DNA fragments containing the L motif of the psp RIB element effectively bind integration host factor (IHF), while the Y palindromic unit (REP1) of the same RIB element binds DNA gyrase weakly. Computer prediction of the pspF mRNA secondary structure suggested that the transcribed stem-loop structures formed by the 3'-flanking region of the pspF transcript containing the RIB element can stabilize and protect pspF mRNA, Analysis of pspF steady-state mRNA levels showed that transcripts,vith an intact RIB element are much more abundant than those truncated at the 3' end by deletion of either the entire RIB element or a single Z(1) sequence (REP2), Thus, the pspF 3'-flanking region containing the RIB element has an important role in the stabilization of the pspF transcript. PB - Amer Soc Microbiology, Washington T2 - Journal of Bacteriology T1 - The RIB element in the goaG-pspF intergenic region of Escherichia coli EP - 3102 IS - 10 SP - 3095 VL - 179 DO - 10.1128/jb.179.10.3095-3102.1997 ER -
@article{ author = "Jovanović, Goran and Model, P", year = "1997", abstract = "The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined, The pspF gene encodes a sigma(54) transcriptional activator of the phage shock protein (psp) operon (pspA to pspE), Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL, and goaG, convergently oriented with respect to pspF, These two ORFs, together with the adjacent aldH gene, may constitute a novel operon (aldH-ordL-goaG), The goaG-pspF intergenic region contains a complex extragenic mosaic element, RIB, The structure of this RIB element, which belongs to the BIME-1 family, is Y(REP1) gt 16 lt Z(1)(REP2), where Y and Z(1) are palindromic units and the central 16 bases contain an L motif with an ihf consensus sequence. DNA fragments containing the L motif of the psp RIB element effectively bind integration host factor (IHF), while the Y palindromic unit (REP1) of the same RIB element binds DNA gyrase weakly. Computer prediction of the pspF mRNA secondary structure suggested that the transcribed stem-loop structures formed by the 3'-flanking region of the pspF transcript containing the RIB element can stabilize and protect pspF mRNA, Analysis of pspF steady-state mRNA levels showed that transcripts,vith an intact RIB element are much more abundant than those truncated at the 3' end by deletion of either the entire RIB element or a single Z(1) sequence (REP2), Thus, the pspF 3'-flanking region containing the RIB element has an important role in the stabilization of the pspF transcript.", publisher = "Amer Soc Microbiology, Washington", journal = "Journal of Bacteriology", title = "The RIB element in the goaG-pspF intergenic region of Escherichia coli", pages = "3102-3095", number = "10", volume = "179", doi = "10.1128/jb.179.10.3095-3102.1997" }
Jovanović, G.,& Model, P.. (1997). The RIB element in the goaG-pspF intergenic region of Escherichia coli. in Journal of Bacteriology Amer Soc Microbiology, Washington., 179(10), 3095-3102. https://doi.org/10.1128/jb.179.10.3095-3102.1997
Jovanović G, Model P. The RIB element in the goaG-pspF intergenic region of Escherichia coli. in Journal of Bacteriology. 1997;179(10):3095-3102. doi:10.1128/jb.179.10.3095-3102.1997 .
Jovanović, Goran, Model, P, "The RIB element in the goaG-pspF intergenic region of Escherichia coli" in Journal of Bacteriology, 179, no. 10 (1997):3095-3102, https://doi.org/10.1128/jb.179.10.3095-3102.1997 . .