In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
Authors
Golić, NatašaVeljović, Katarina
Popović, Nikola
Đokić, Jelena
Strahinić, Ivana
Mrvaljević, Igor
Terzić-Vidojević, Amarela
Article (Published version)
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Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymph...ocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.
Keywords:
TGF-beta / Probiotic / Goats / GALT / Clostridium sp.Source:
BMC Microbiology, 2017, 17Publisher:
- BMC, London
Funding / projects:
- Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1186/s12866-017-1015-5
ISSN: 1471-2180
PubMed: 28477627
WoS: 000401564800002
Scopus: 2-s2.0-85018741414
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Golić, Nataša AU - Veljović, Katarina AU - Popović, Nikola AU - Đokić, Jelena AU - Strahinić, Ivana AU - Mrvaljević, Igor AU - Terzić-Vidojević, Amarela PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1091 AB - Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens. PB - BMC, London T2 - BMC Microbiology T1 - In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens VL - 17 DO - 10.1186/s12866-017-1015-5 ER -
@article{ author = "Golić, Nataša and Veljović, Katarina and Popović, Nikola and Đokić, Jelena and Strahinić, Ivana and Mrvaljević, Igor and Terzić-Vidojević, Amarela", year = "2017", abstract = "Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.", publisher = "BMC, London", journal = "BMC Microbiology", title = "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens", volume = "17", doi = "10.1186/s12866-017-1015-5" }
Golić, N., Veljović, K., Popović, N., Đokić, J., Strahinić, I., Mrvaljević, I.,& Terzić-Vidojević, A.. (2017). In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology BMC, London., 17. https://doi.org/10.1186/s12866-017-1015-5
Golić N, Veljović K, Popović N, Đokić J, Strahinić I, Mrvaljević I, Terzić-Vidojević A. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology. 2017;17. doi:10.1186/s12866-017-1015-5 .
Golić, Nataša, Veljović, Katarina, Popović, Nikola, Đokić, Jelena, Strahinić, Ivana, Mrvaljević, Igor, Terzić-Vidojević, Amarela, "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens" in BMC Microbiology, 17 (2017), https://doi.org/10.1186/s12866-017-1015-5 . .