Short-term fasting promotes insulin expression in rat hypothalamus
Нема приказа
Аутори
Dakić, Tamara B.Jevdjović, Tanja V.
Perić, Mina
Bjelobaba, Ivana M.
Markelić, Milica B.
Milutinović, Bojana S.
Lakić, Iva V.
Jasnić, Nebojša I.
Đorđević, Jelena
Vujović, Predrag Z.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of f...asting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.
Кључне речи:
rat / insulin / hypothalamus / glucose / fastingИзвор:
European Journal of Neuroscience, 2017, 46, 1, 1730-1737Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Ефекат метаболичких и неметаболичких стресора на експресију и деловање неуроендокриних регулатора енергетске хомеостазе (RS-MESTD-Basic Research (BR or ON)-173023)
DOI: 10.1111/ejn.13607
ISSN: 0953-816X
PubMed: 28544147
WoS: 000404875600011
Scopus: 2-s2.0-85020389087
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Dakić, Tamara B. AU - Jevdjović, Tanja V. AU - Perić, Mina AU - Bjelobaba, Ivana M. AU - Markelić, Milica B. AU - Milutinović, Bojana S. AU - Lakić, Iva V. AU - Jasnić, Nebojša I. AU - Đorđević, Jelena AU - Vujović, Predrag Z. PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1094 AB - In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular. PB - Wiley, Hoboken T2 - European Journal of Neuroscience T1 - Short-term fasting promotes insulin expression in rat hypothalamus EP - 1737 IS - 1 SP - 1730 VL - 46 DO - 10.1111/ejn.13607 ER -
@article{ author = "Dakić, Tamara B. and Jevdjović, Tanja V. and Perić, Mina and Bjelobaba, Ivana M. and Markelić, Milica B. and Milutinović, Bojana S. and Lakić, Iva V. and Jasnić, Nebojša I. and Đorđević, Jelena and Vujović, Predrag Z.", year = "2017", abstract = "In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.", publisher = "Wiley, Hoboken", journal = "European Journal of Neuroscience", title = "Short-term fasting promotes insulin expression in rat hypothalamus", pages = "1737-1730", number = "1", volume = "46", doi = "10.1111/ejn.13607" }
Dakić, T. B., Jevdjović, T. V., Perić, M., Bjelobaba, I. M., Markelić, M. B., Milutinović, B. S., Lakić, I. V., Jasnić, N. I., Đorđević, J.,& Vujović, P. Z.. (2017). Short-term fasting promotes insulin expression in rat hypothalamus. in European Journal of Neuroscience Wiley, Hoboken., 46(1), 1730-1737. https://doi.org/10.1111/ejn.13607
Dakić TB, Jevdjović TV, Perić M, Bjelobaba IM, Markelić MB, Milutinović BS, Lakić IV, Jasnić NI, Đorđević J, Vujović PZ. Short-term fasting promotes insulin expression in rat hypothalamus. in European Journal of Neuroscience. 2017;46(1):1730-1737. doi:10.1111/ejn.13607 .
Dakić, Tamara B., Jevdjović, Tanja V., Perić, Mina, Bjelobaba, Ivana M., Markelić, Milica B., Milutinović, Bojana S., Lakić, Iva V., Jasnić, Nebojša I., Đorđević, Jelena, Vujović, Predrag Z., "Short-term fasting promotes insulin expression in rat hypothalamus" in European Journal of Neuroscience, 46, no. 1 (2017):1730-1737, https://doi.org/10.1111/ejn.13607 . .