Association between the-174 C/G polymorphism in the interleukin-6 (IL-6) gene and gastrointestinal involvement in patients with systemic sclerosis
Нема приказа
Аутори
Zeković, AnaVreca, Misa
Spasovski, Vesna
Anđelković, Marina
Pavlović, Sonja
Damjanov, Nemanja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Genetic predisposition to systemic sclerosis (SSc) has still not been fully revealed. Interleukin-6 (IL-6) is a mediator of T cell proliferation and fibrotic events in SSc. Polymorphisms in the IL-6 are found to be important in susceptibility to development of SSc. We aimed to assess the frequency of -174 C/G of IL-6 gene polymorphism in SSc patients and healthy controls, as well as correlation with disease manifestations. In the case-control study, 102 patients with SSc and 93 controls were included. PCR-RFLP method was performed for genotyping promotor variants -174 C/G of IL-6 gene. The expression level of IL-6 was determined by qRT-PCR on subset of 50 patients and 13 healthy controls with different IL-6 genotypes. We used UCLA GIT 2.0 questionnaire to assess gastrointestinal involvement in SSc patients. The expression level of IL-6 gene was significantly higher in patients with SSc in comparison with healthy controls (p lt 0.05). Carriers of C-allele of IL-6 gene compared to thos...e with G allele, showed higher expression of IL-6 gene (95.8 vs. 41.2, p lt 0.05), higher GIT total score (0.85 vs. 0.5, p lt 0.05) and higher distension scale score (1.4 +/- 0.9 vs. 0.78 +/- 0.8, p = 0.05). No significant differences in genotype distribution and allele frequency were observed between patients and controls. The expression of IL6 gene varies significantly during the course of SSc. The IL-6 gene variant -174 C/G (presence of C-allele) is associated with higher IL-6 gene expression and greater GIT impairment in patients with SSc.
Кључне речи:
Systemic sclerosis / IL6-gene polymorphism / GI involvementИзвор:
Clinical Rheumatology, 2018, 37, 9, 2447-2454Издавач:
- Springer London Ltd, London
Финансирање / пројекти:
- Ретке болести: молекуларна патофизиологија, дијагностички и терапијски модалитети и социјални, етички и правни аспекти (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41004)
- Клиничко епидемиолошка истраживања најчешћих нежељених догађаја током болничког лечења (RS-MESTD-Basic Research (BR or ON)-175046)
DOI: 10.1007/s10067-018-4163-6
ISSN: 0770-3198
PubMed: 29948346
WoS: 000441845200017
Scopus: 2-s2.0-85048306683
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Zeković, Ana AU - Vreca, Misa AU - Spasovski, Vesna AU - Anđelković, Marina AU - Pavlović, Sonja AU - Damjanov, Nemanja PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1102 AB - Genetic predisposition to systemic sclerosis (SSc) has still not been fully revealed. Interleukin-6 (IL-6) is a mediator of T cell proliferation and fibrotic events in SSc. Polymorphisms in the IL-6 are found to be important in susceptibility to development of SSc. We aimed to assess the frequency of -174 C/G of IL-6 gene polymorphism in SSc patients and healthy controls, as well as correlation with disease manifestations. In the case-control study, 102 patients with SSc and 93 controls were included. PCR-RFLP method was performed for genotyping promotor variants -174 C/G of IL-6 gene. The expression level of IL-6 was determined by qRT-PCR on subset of 50 patients and 13 healthy controls with different IL-6 genotypes. We used UCLA GIT 2.0 questionnaire to assess gastrointestinal involvement in SSc patients. The expression level of IL-6 gene was significantly higher in patients with SSc in comparison with healthy controls (p lt 0.05). Carriers of C-allele of IL-6 gene compared to those with G allele, showed higher expression of IL-6 gene (95.8 vs. 41.2, p lt 0.05), higher GIT total score (0.85 vs. 0.5, p lt 0.05) and higher distension scale score (1.4 +/- 0.9 vs. 0.78 +/- 0.8, p = 0.05). No significant differences in genotype distribution and allele frequency were observed between patients and controls. The expression of IL6 gene varies significantly during the course of SSc. The IL-6 gene variant -174 C/G (presence of C-allele) is associated with higher IL-6 gene expression and greater GIT impairment in patients with SSc. PB - Springer London Ltd, London T2 - Clinical Rheumatology T1 - Association between the-174 C/G polymorphism in the interleukin-6 (IL-6) gene and gastrointestinal involvement in patients with systemic sclerosis EP - 2454 IS - 9 SP - 2447 VL - 37 DO - 10.1007/s10067-018-4163-6 ER -
@article{ author = "Zeković, Ana and Vreca, Misa and Spasovski, Vesna and Anđelković, Marina and Pavlović, Sonja and Damjanov, Nemanja", year = "2018", abstract = "Genetic predisposition to systemic sclerosis (SSc) has still not been fully revealed. Interleukin-6 (IL-6) is a mediator of T cell proliferation and fibrotic events in SSc. Polymorphisms in the IL-6 are found to be important in susceptibility to development of SSc. We aimed to assess the frequency of -174 C/G of IL-6 gene polymorphism in SSc patients and healthy controls, as well as correlation with disease manifestations. In the case-control study, 102 patients with SSc and 93 controls were included. PCR-RFLP method was performed for genotyping promotor variants -174 C/G of IL-6 gene. The expression level of IL-6 was determined by qRT-PCR on subset of 50 patients and 13 healthy controls with different IL-6 genotypes. We used UCLA GIT 2.0 questionnaire to assess gastrointestinal involvement in SSc patients. The expression level of IL-6 gene was significantly higher in patients with SSc in comparison with healthy controls (p lt 0.05). Carriers of C-allele of IL-6 gene compared to those with G allele, showed higher expression of IL-6 gene (95.8 vs. 41.2, p lt 0.05), higher GIT total score (0.85 vs. 0.5, p lt 0.05) and higher distension scale score (1.4 +/- 0.9 vs. 0.78 +/- 0.8, p = 0.05). No significant differences in genotype distribution and allele frequency were observed between patients and controls. The expression of IL6 gene varies significantly during the course of SSc. The IL-6 gene variant -174 C/G (presence of C-allele) is associated with higher IL-6 gene expression and greater GIT impairment in patients with SSc.", publisher = "Springer London Ltd, London", journal = "Clinical Rheumatology", title = "Association between the-174 C/G polymorphism in the interleukin-6 (IL-6) gene and gastrointestinal involvement in patients with systemic sclerosis", pages = "2454-2447", number = "9", volume = "37", doi = "10.1007/s10067-018-4163-6" }
Zeković, A., Vreca, M., Spasovski, V., Anđelković, M., Pavlović, S.,& Damjanov, N.. (2018). Association between the-174 C/G polymorphism in the interleukin-6 (IL-6) gene and gastrointestinal involvement in patients with systemic sclerosis. in Clinical Rheumatology Springer London Ltd, London., 37(9), 2447-2454. https://doi.org/10.1007/s10067-018-4163-6
Zeković A, Vreca M, Spasovski V, Anđelković M, Pavlović S, Damjanov N. Association between the-174 C/G polymorphism in the interleukin-6 (IL-6) gene and gastrointestinal involvement in patients with systemic sclerosis. in Clinical Rheumatology. 2018;37(9):2447-2454. doi:10.1007/s10067-018-4163-6 .
Zeković, Ana, Vreca, Misa, Spasovski, Vesna, Anđelković, Marina, Pavlović, Sonja, Damjanov, Nemanja, "Association between the-174 C/G polymorphism in the interleukin-6 (IL-6) gene and gastrointestinal involvement in patients with systemic sclerosis" in Clinical Rheumatology, 37, no. 9 (2018):2447-2454, https://doi.org/10.1007/s10067-018-4163-6 . .