Kinetic profile of the antioxidant activity of propolis extract: 2,2-diphenyl-1-picrylhydrazyl radical bleaching assay
2018
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Аутори
Mirković, SnjezanaRajković, Katarina
Jeremić, Sanja
Gavrilović, Marijana
Tomić, Ljiljana
Arsić-Arsenijević, Valentina
Krstić, Boro
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH center dot) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH center dot for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (k(2)) for the oxidation of PE by DPPH center dot, determined for the first time in this study, was 0.17 dm(3)g(-1)s(-1). The obtained k(2) value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the k(2) was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The k(2) parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analy...sis should become a necessary step for more precise antioxidative characterization of propolis.
Кључне речи:
second order rate constant / proplis extract / kinetic analysis / antioxidative activityИзвор:
Journal of Apicultural Science, 2018, 62, 1, 39-46Издавач:
- Research Inst Pomology Floriculture, Div Apiculture, Pulawy
Финансирање / пројекти:
- Значај доказивања раних лабораторијских биомаркера за исход инвазивних гљивичних инфекција код нас (RS-MESTD-Basic Research (BR or ON)-175034)
DOI: 10.2478/JAS-2018-0004
ISSN: 1643-4439
WoS: 000436260000004
Scopus: 2-s2.0-85054841268
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mirković, Snjezana AU - Rajković, Katarina AU - Jeremić, Sanja AU - Gavrilović, Marijana AU - Tomić, Ljiljana AU - Arsić-Arsenijević, Valentina AU - Krstić, Boro PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1112 AB - The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH center dot) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH center dot for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (k(2)) for the oxidation of PE by DPPH center dot, determined for the first time in this study, was 0.17 dm(3)g(-1)s(-1). The obtained k(2) value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the k(2) was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The k(2) parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analysis should become a necessary step for more precise antioxidative characterization of propolis. PB - Research Inst Pomology Floriculture, Div Apiculture, Pulawy T2 - Journal of Apicultural Science T1 - Kinetic profile of the antioxidant activity of propolis extract: 2,2-diphenyl-1-picrylhydrazyl radical bleaching assay EP - 46 IS - 1 SP - 39 VL - 62 DO - 10.2478/JAS-2018-0004 ER -
@article{ author = "Mirković, Snjezana and Rajković, Katarina and Jeremić, Sanja and Gavrilović, Marijana and Tomić, Ljiljana and Arsić-Arsenijević, Valentina and Krstić, Boro", year = "2018", abstract = "The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH center dot) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH center dot for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (k(2)) for the oxidation of PE by DPPH center dot, determined for the first time in this study, was 0.17 dm(3)g(-1)s(-1). The obtained k(2) value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the k(2) was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The k(2) parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analysis should become a necessary step for more precise antioxidative characterization of propolis.", publisher = "Research Inst Pomology Floriculture, Div Apiculture, Pulawy", journal = "Journal of Apicultural Science", title = "Kinetic profile of the antioxidant activity of propolis extract: 2,2-diphenyl-1-picrylhydrazyl radical bleaching assay", pages = "46-39", number = "1", volume = "62", doi = "10.2478/JAS-2018-0004" }
Mirković, S., Rajković, K., Jeremić, S., Gavrilović, M., Tomić, L., Arsić-Arsenijević, V.,& Krstić, B.. (2018). Kinetic profile of the antioxidant activity of propolis extract: 2,2-diphenyl-1-picrylhydrazyl radical bleaching assay. in Journal of Apicultural Science Research Inst Pomology Floriculture, Div Apiculture, Pulawy., 62(1), 39-46. https://doi.org/10.2478/JAS-2018-0004
Mirković S, Rajković K, Jeremić S, Gavrilović M, Tomić L, Arsić-Arsenijević V, Krstić B. Kinetic profile of the antioxidant activity of propolis extract: 2,2-diphenyl-1-picrylhydrazyl radical bleaching assay. in Journal of Apicultural Science. 2018;62(1):39-46. doi:10.2478/JAS-2018-0004 .
Mirković, Snjezana, Rajković, Katarina, Jeremić, Sanja, Gavrilović, Marijana, Tomić, Ljiljana, Arsić-Arsenijević, Valentina, Krstić, Boro, "Kinetic profile of the antioxidant activity of propolis extract: 2,2-diphenyl-1-picrylhydrazyl radical bleaching assay" in Journal of Apicultural Science, 62, no. 1 (2018):39-46, https://doi.org/10.2478/JAS-2018-0004 . .