Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans
2018
Аутори
Milivojević, DušanSumonja, Neven
Medić, Strahinja
Pavić, Aleksandar
Morić, Ivana
Vasiljević, Branka
Šenerović, Lidija
Nikodinović-Runić, Jasmina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predict...ive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability.
Кључне речи:
virulence factors / Pseudomonas aeruginosa / infection / human isolates / biofilm / animal isolatesИзвор:
Pathogens and Disease, 2018, 76, 4Издавач:
- Oxford Univ Press, Oxford
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
- Примена EIIP/ISM биоинформатичке платформе у откривању нових терапеутских таргета и потенцијалних терапеутских молекула (RS-MESTD-Basic Research (BR or ON)-173001)
DOI: 10.1093/femspd/fty041
ISSN: 2049-632X
PubMed: 29684116
WoS: 000439774500015
Scopus: 2-s2.0-85050798463
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Milivojević, Dušan AU - Sumonja, Neven AU - Medić, Strahinja AU - Pavić, Aleksandar AU - Morić, Ivana AU - Vasiljević, Branka AU - Šenerović, Lidija AU - Nikodinović-Runić, Jasmina PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1117 AB - Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability. PB - Oxford Univ Press, Oxford T2 - Pathogens and Disease T1 - Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans IS - 4 VL - 76 DO - 10.1093/femspd/fty041 ER -
@article{ author = "Milivojević, Dušan and Sumonja, Neven and Medić, Strahinja and Pavić, Aleksandar and Morić, Ivana and Vasiljević, Branka and Šenerović, Lidija and Nikodinović-Runić, Jasmina", year = "2018", abstract = "Pseudomonas aeruginosa has been amongst the top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human), 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysin production, and bacterial motility patterns were compared with the ability to kill human cell line A549 in vitro. On average, there was no significant difference between levels of animal and human cytotoxicity, while human isolates produced higher amounts of pyocyanin, hemolysins and showed increased swimming ability. Non-parametric statistical analysis identified the highest positive correlation between hemolysis and the swarming ability. For the first time an ensemble machine learning approach used on the in vitro virulence data determined the highest relative predictive importance of the submerged biofilm formation for the cytotoxicity, as an indicator of the infection ability. The findings from the in vitro study were validated in vivo using zebrafish (Danio rerio) embryos. This study highlighted no major differences between P. aeruginosa species isolated from animal and human infections and the importance of pyocyanin production in cytotoxicity and infection ability.", publisher = "Oxford Univ Press, Oxford", journal = "Pathogens and Disease", title = "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans", number = "4", volume = "76", doi = "10.1093/femspd/fty041" }
Milivojević, D., Sumonja, N., Medić, S., Pavić, A., Morić, I., Vasiljević, B., Šenerović, L.,& Nikodinović-Runić, J.. (2018). Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease Oxford Univ Press, Oxford., 76(4). https://doi.org/10.1093/femspd/fty041
Milivojević D, Sumonja N, Medić S, Pavić A, Morić I, Vasiljević B, Šenerović L, Nikodinović-Runić J. Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans. in Pathogens and Disease. 2018;76(4). doi:10.1093/femspd/fty041 .
Milivojević, Dušan, Sumonja, Neven, Medić, Strahinja, Pavić, Aleksandar, Morić, Ivana, Vasiljević, Branka, Šenerović, Lidija, Nikodinović-Runić, Jasmina, "Biofilm-forming ability and infection potential of Pseudomonas aeruginosa strains isolated from animals and humans" in Pathogens and Disease, 76, no. 4 (2018), https://doi.org/10.1093/femspd/fty041 . .