Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study
Authors
Dragasević, SanjaStanković, Biljana
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Sokić-Milutinović, Aleksandra
Milosavljević, Tomica
Milovanović, Tamara
Lukić, Snežana
Srzentić Dražilov, Sanja
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Klaassen, Kristel
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Kotur, Nikola
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Pavlović, Sonja
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Popović, Dragan
Article (Accepted Version)
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Background and aims: Mucosal gene expression have not been fully enlightened in inflammatory bowel disease (IBD). Aim of this study was to define IL23A, IL17A, IL17F and TLR9 expression in different IBD phenotypes. Methods: Evaluation of mRNA levels was performed in paired non-inflamed and inflamed mucosal biopsies of newly diagnosed 50 Crohn's disease (CD) and 54 ulcerative colitis (UC) patients by quantitative real-time PCR analysis. Results: IL17A and IL17F expression levels were significantly increased in inflamed IBD mucosa. Inflamed CD ileal and UC mucosa showed increased IL23A, while only inflamed CD ileal samples showed increased TLR9 mRNA level. Correlation between analysed mRNAs levels and endoscopic and clinical disease activity were found in UC, but only with clinical activity in CD. Conclusion: Both CD and UC presented expression of Th17-associated genes. Nevertheless, expression profiles between different disease forms varies which should be taken into account for future ...research and therapeutics strategies.
Keywords:
Inflammatory bowel disease / IL23/IL17/TLR9 / Expression profilingSource:
Clinical Immunology, 2018, 197, 86-95Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- Rare Diseases:Molecular Pathophysiology, Diagnostic and Therapeutic Modalities and Social, Ethical and Legal Aspects (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41004)
Note:
- This is the peer reviewed version of the paper: Dragasevic, S., Stankovic, B., Sokic-Milutinovic, A., Milosavljevic, T., Milovanovic, T., Lukic, S., Drazilov, S. S., Klaassen, K., Kotur, N., Pavlovic, S., & Popovic, D. (2018). Importance of TLR9-IL23-IL17 axis in inflammatory bowel disease development: Gene expression profiling study. Clinical Immunology, 197, 86–95. https://doi.org/10.1016/j.clim.2018.09.001
Related info:
- Version of
https://doi.org/10.1016/j.clim.2018.09.001
DOI: 10.1016/j.clim.2018.09.001
ISSN: 1521-6616
PubMed: 30193869
WoS: 000454372500012
Scopus: 2-s2.0-85053560716
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Dragasević, Sanja AU - Stanković, Biljana AU - Sokić-Milutinović, Aleksandra AU - Milosavljević, Tomica AU - Milovanović, Tamara AU - Lukić, Snežana AU - Srzentić Dražilov, Sanja AU - Klaassen, Kristel AU - Kotur, Nikola AU - Pavlović, Sonja AU - Popović, Dragan PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1123 AB - Background and aims: Mucosal gene expression have not been fully enlightened in inflammatory bowel disease (IBD). Aim of this study was to define IL23A, IL17A, IL17F and TLR9 expression in different IBD phenotypes. Methods: Evaluation of mRNA levels was performed in paired non-inflamed and inflamed mucosal biopsies of newly diagnosed 50 Crohn's disease (CD) and 54 ulcerative colitis (UC) patients by quantitative real-time PCR analysis. Results: IL17A and IL17F expression levels were significantly increased in inflamed IBD mucosa. Inflamed CD ileal and UC mucosa showed increased IL23A, while only inflamed CD ileal samples showed increased TLR9 mRNA level. Correlation between analysed mRNAs levels and endoscopic and clinical disease activity were found in UC, but only with clinical activity in CD. Conclusion: Both CD and UC presented expression of Th17-associated genes. Nevertheless, expression profiles between different disease forms varies which should be taken into account for future research and therapeutics strategies. PB - Academic Press Inc Elsevier Science, San Diego T2 - Clinical Immunology T1 - Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study EP - 95 SP - 86 VL - 197 DO - 10.1016/j.clim.2018.09.001 ER -
@article{ author = "Dragasević, Sanja and Stanković, Biljana and Sokić-Milutinović, Aleksandra and Milosavljević, Tomica and Milovanović, Tamara and Lukić, Snežana and Srzentić Dražilov, Sanja and Klaassen, Kristel and Kotur, Nikola and Pavlović, Sonja and Popović, Dragan", year = "2018", abstract = "Background and aims: Mucosal gene expression have not been fully enlightened in inflammatory bowel disease (IBD). Aim of this study was to define IL23A, IL17A, IL17F and TLR9 expression in different IBD phenotypes. Methods: Evaluation of mRNA levels was performed in paired non-inflamed and inflamed mucosal biopsies of newly diagnosed 50 Crohn's disease (CD) and 54 ulcerative colitis (UC) patients by quantitative real-time PCR analysis. Results: IL17A and IL17F expression levels were significantly increased in inflamed IBD mucosa. Inflamed CD ileal and UC mucosa showed increased IL23A, while only inflamed CD ileal samples showed increased TLR9 mRNA level. Correlation between analysed mRNAs levels and endoscopic and clinical disease activity were found in UC, but only with clinical activity in CD. Conclusion: Both CD and UC presented expression of Th17-associated genes. Nevertheless, expression profiles between different disease forms varies which should be taken into account for future research and therapeutics strategies.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Clinical Immunology", title = "Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study", pages = "95-86", volume = "197", doi = "10.1016/j.clim.2018.09.001" }
Dragasević, S., Stanković, B., Sokić-Milutinović, A., Milosavljević, T., Milovanović, T., Lukić, S., Srzentić Dražilov, S., Klaassen, K., Kotur, N., Pavlović, S.,& Popović, D.. (2018). Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study. in Clinical Immunology Academic Press Inc Elsevier Science, San Diego., 197, 86-95. https://doi.org/10.1016/j.clim.2018.09.001
Dragasević S, Stanković B, Sokić-Milutinović A, Milosavljević T, Milovanović T, Lukić S, Srzentić Dražilov S, Klaassen K, Kotur N, Pavlović S, Popović D. Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study. in Clinical Immunology. 2018;197:86-95. doi:10.1016/j.clim.2018.09.001 .
Dragasević, Sanja, Stanković, Biljana, Sokić-Milutinović, Aleksandra, Milosavljević, Tomica, Milovanović, Tamara, Lukić, Snežana, Srzentić Dražilov, Sanja, Klaassen, Kristel, Kotur, Nikola, Pavlović, Sonja, Popović, Dragan, "Importance of TLR9-IL23-1L17 axis in inflammatory bowel disease development: Gene expression profiling study" in Clinical Immunology, 197 (2018):86-95, https://doi.org/10.1016/j.clim.2018.09.001 . .