Den 1, den 2 and den 3, ATP-inhibited deoxyribonucleases from Dropsophila embryonic nuclei
Samo za registrovane korisnike
1998
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Three Drosophila embryonic deoxyribonucleases, designated den1, den2 and den3, are identified in nuclear extracts separated by glycerol density gradient centrifugation. Den1, removes short products from the 5'-ends of single-stranded DNA or double-stranded DNA with either blunt or 5'-recessed termini. Den2 is inactive with single-stranded DNA and acts as 3'-exonuclease with double-stranded DNA possessing either blunt or 3'-recessed termini. Den3 preferentially uses partial duplex DNA containing single-stranded gap and it catalyzes hydrolysis, in 3'-5' direction, of one of the shorter strands that flank the gap. Nucleolytic activities of den1, den2 and den3 are inhibited with ATP.
Ključne reči:
nuclear deoxyribonucleases / Drosophila embryos / ATP-inhibitedIzvor:
Molecular and Cellular Biochemistry, 1998, 189, 1-2, 207-212Izdavač:
- Springer, Dordrecht
DOI: 10.1023/A:1006879132158
ISSN: 0300-8177
PubMed: 9879673
WoS: 000077769800027
Scopus: 2-s2.0-0031733247
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Snežana AU - Todorović, V AU - Ristić, D AU - Savić, A AU - Stefanović, D PY - 1998 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/115 AB - Three Drosophila embryonic deoxyribonucleases, designated den1, den2 and den3, are identified in nuclear extracts separated by glycerol density gradient centrifugation. Den1, removes short products from the 5'-ends of single-stranded DNA or double-stranded DNA with either blunt or 5'-recessed termini. Den2 is inactive with single-stranded DNA and acts as 3'-exonuclease with double-stranded DNA possessing either blunt or 3'-recessed termini. Den3 preferentially uses partial duplex DNA containing single-stranded gap and it catalyzes hydrolysis, in 3'-5' direction, of one of the shorter strands that flank the gap. Nucleolytic activities of den1, den2 and den3 are inhibited with ATP. PB - Springer, Dordrecht T2 - Molecular and Cellular Biochemistry T1 - Den 1, den 2 and den 3, ATP-inhibited deoxyribonucleases from Dropsophila embryonic nuclei EP - 212 IS - 1-2 SP - 207 VL - 189 DO - 10.1023/A:1006879132158 ER -
@article{ author = "Kojić, Snežana and Todorović, V and Ristić, D and Savić, A and Stefanović, D", year = "1998", abstract = "Three Drosophila embryonic deoxyribonucleases, designated den1, den2 and den3, are identified in nuclear extracts separated by glycerol density gradient centrifugation. Den1, removes short products from the 5'-ends of single-stranded DNA or double-stranded DNA with either blunt or 5'-recessed termini. Den2 is inactive with single-stranded DNA and acts as 3'-exonuclease with double-stranded DNA possessing either blunt or 3'-recessed termini. Den3 preferentially uses partial duplex DNA containing single-stranded gap and it catalyzes hydrolysis, in 3'-5' direction, of one of the shorter strands that flank the gap. Nucleolytic activities of den1, den2 and den3 are inhibited with ATP.", publisher = "Springer, Dordrecht", journal = "Molecular and Cellular Biochemistry", title = "Den 1, den 2 and den 3, ATP-inhibited deoxyribonucleases from Dropsophila embryonic nuclei", pages = "212-207", number = "1-2", volume = "189", doi = "10.1023/A:1006879132158" }
Kojić, S., Todorović, V., Ristić, D., Savić, A.,& Stefanović, D.. (1998). Den 1, den 2 and den 3, ATP-inhibited deoxyribonucleases from Dropsophila embryonic nuclei. in Molecular and Cellular Biochemistry Springer, Dordrecht., 189(1-2), 207-212. https://doi.org/10.1023/A:1006879132158
Kojić S, Todorović V, Ristić D, Savić A, Stefanović D. Den 1, den 2 and den 3, ATP-inhibited deoxyribonucleases from Dropsophila embryonic nuclei. in Molecular and Cellular Biochemistry. 1998;189(1-2):207-212. doi:10.1023/A:1006879132158 .
Kojić, Snežana, Todorović, V, Ristić, D, Savić, A, Stefanović, D, "Den 1, den 2 and den 3, ATP-inhibited deoxyribonucleases from Dropsophila embryonic nuclei" in Molecular and Cellular Biochemistry, 189, no. 1-2 (1998):207-212, https://doi.org/10.1023/A:1006879132158 . .