Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties
2018
Аутори
Kanelli, MariaMandić, Mina
Kalakona, Margarita
Vasilakos, Sozon
Kekos, Dimitris
Nikodinović-Runić, Jasmina
Topakas, Evangelos
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In the present study, crude bacterial extract containing violacein is investigated for the preparation of antimicrobial polyamide fabrics. The optimal culture conditions of Janthinobacterium lividum (JL) for maximum biomass and violacein production were found to be 25 degrees C, pH 7.0, while the addition of ampicillin of 0.2 mg mL(-1) in the small scale increased violacein production 1.3-fold. In scale-up trials, the addition of 1% (v/v) glycerol in a fed-batch bioreactor, resulted in fivefold extracted crude violacein increase with final concentration of 1.828 g L-1. Polyamide 6.6 fabrics were dyed following three different processes; through simultaneous fermentation and dyeing (SFD), by incubating the fabric in the sonicated bacterial culture after fermentation and by using cell-free extract containing violacein. Maximum color change (Delta E) and color strength (K/S) obtained for SFD fabrics were 74.81 and 22.01, respectively, while no alteration of fastness and staining of dye at... acid and alkaline perspiration or at water was indicated. The dyed fabrics presented significant antifungal activity against Candida albicans, C. parapsilosis, and C. krusei, as well as antibacterial properties against Escherichia coli, Staphylococcus aureus, and the S. aureus MRSA. We have shown that J. lividum cultures can be successfully used for violacein production and for simultaneous dying of fabrics resulting in dyed fabrics with antimicrobial properties without utilization of organic solvents.
Кључне речи:
violacein / polyamide / Janthinobacterium lividum / bacterial pigment / antimicrobial activityИзвор:
Frontiers in Microbiology, 2018, 9Издавач:
- Frontiers Media Sa, Lausanne
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.3389/fmicb.2018.01495
ISSN: 1664-302X
WoS: 000438066200002
Scopus: 2-s2.0-85049834158
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kanelli, Maria AU - Mandić, Mina AU - Kalakona, Margarita AU - Vasilakos, Sozon AU - Kekos, Dimitris AU - Nikodinović-Runić, Jasmina AU - Topakas, Evangelos PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1160 AB - In the present study, crude bacterial extract containing violacein is investigated for the preparation of antimicrobial polyamide fabrics. The optimal culture conditions of Janthinobacterium lividum (JL) for maximum biomass and violacein production were found to be 25 degrees C, pH 7.0, while the addition of ampicillin of 0.2 mg mL(-1) in the small scale increased violacein production 1.3-fold. In scale-up trials, the addition of 1% (v/v) glycerol in a fed-batch bioreactor, resulted in fivefold extracted crude violacein increase with final concentration of 1.828 g L-1. Polyamide 6.6 fabrics were dyed following three different processes; through simultaneous fermentation and dyeing (SFD), by incubating the fabric in the sonicated bacterial culture after fermentation and by using cell-free extract containing violacein. Maximum color change (Delta E) and color strength (K/S) obtained for SFD fabrics were 74.81 and 22.01, respectively, while no alteration of fastness and staining of dye at acid and alkaline perspiration or at water was indicated. The dyed fabrics presented significant antifungal activity against Candida albicans, C. parapsilosis, and C. krusei, as well as antibacterial properties against Escherichia coli, Staphylococcus aureus, and the S. aureus MRSA. We have shown that J. lividum cultures can be successfully used for violacein production and for simultaneous dying of fabrics resulting in dyed fabrics with antimicrobial properties without utilization of organic solvents. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties VL - 9 DO - 10.3389/fmicb.2018.01495 ER -
@article{ author = "Kanelli, Maria and Mandić, Mina and Kalakona, Margarita and Vasilakos, Sozon and Kekos, Dimitris and Nikodinović-Runić, Jasmina and Topakas, Evangelos", year = "2018", abstract = "In the present study, crude bacterial extract containing violacein is investigated for the preparation of antimicrobial polyamide fabrics. The optimal culture conditions of Janthinobacterium lividum (JL) for maximum biomass and violacein production were found to be 25 degrees C, pH 7.0, while the addition of ampicillin of 0.2 mg mL(-1) in the small scale increased violacein production 1.3-fold. In scale-up trials, the addition of 1% (v/v) glycerol in a fed-batch bioreactor, resulted in fivefold extracted crude violacein increase with final concentration of 1.828 g L-1. Polyamide 6.6 fabrics were dyed following three different processes; through simultaneous fermentation and dyeing (SFD), by incubating the fabric in the sonicated bacterial culture after fermentation and by using cell-free extract containing violacein. Maximum color change (Delta E) and color strength (K/S) obtained for SFD fabrics were 74.81 and 22.01, respectively, while no alteration of fastness and staining of dye at acid and alkaline perspiration or at water was indicated. The dyed fabrics presented significant antifungal activity against Candida albicans, C. parapsilosis, and C. krusei, as well as antibacterial properties against Escherichia coli, Staphylococcus aureus, and the S. aureus MRSA. We have shown that J. lividum cultures can be successfully used for violacein production and for simultaneous dying of fabrics resulting in dyed fabrics with antimicrobial properties without utilization of organic solvents.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties", volume = "9", doi = "10.3389/fmicb.2018.01495" }
Kanelli, M., Mandić, M., Kalakona, M., Vasilakos, S., Kekos, D., Nikodinović-Runić, J.,& Topakas, E.. (2018). Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 9. https://doi.org/10.3389/fmicb.2018.01495
Kanelli M, Mandić M, Kalakona M, Vasilakos S, Kekos D, Nikodinović-Runić J, Topakas E. Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties. in Frontiers in Microbiology. 2018;9. doi:10.3389/fmicb.2018.01495 .
Kanelli, Maria, Mandić, Mina, Kalakona, Margarita, Vasilakos, Sozon, Kekos, Dimitris, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Microbial Production of Violacein and Process Optimization for Dyeing Polyamide Fabrics With Acquired Antimicrobial Properties" in Frontiers in Microbiology, 9 (2018), https://doi.org/10.3389/fmicb.2018.01495 . .