Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina
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Аутори
Elez-Burnjaković, NikolinaUgrin, Milena
Obradović, Jasmina
Miletić, Nataša
Racić, Maja
Kulić, Milan
Pavlović, Sonja
Jurisić, Vladimir
Чланак у часопису (Објављена верзија)
Метаподаци
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Purpose: To analyze the frequencies of two single nucleotide polymorphisms (SNPs) of EGFR gene, -191C/A and 181946G/A, among lung cancer patients from the Republic of Srpska, Bosnia and Hercegovina, as well as to assess the association of SNP genotypes with the cancer type and other demographic characteristics of patients, particularly with the smoking status. Methods: This study enrolled 41 lung cancer patients from the territory of Republic Srpska, Bosnia and Herzegovina. Detection EGFR SNPs was performed using PCR-RFLP methodology. PCR was performed on 2720 Thermal Cycler (Applied Biosystems, United States). PCR, as well as RFLP products, were detected by gel electrophoresis. SPSS-17 soft-ware (SPSS, Inc.) was used for statistical analyses. Results: There was significantly more male than female smokers in our cohort (p=0.006). In addition, the proportion of smokers was higher among patients with adenocarcinoma in comparison to patients with other lung cancer types p=0.044). Adenocar...cinoma was less common in patients older than 64 years (p=0.035). The wild type homozygous genotype of both SNPs was the most frequent genotype in all the tested demographic groups. Using dominant genetic model for -191C/A SNP, we observed statistically significant association of -191CC genotype and adenocarcinoma (p=0.043) in the subgroup of patients younger than 64 years. Namely, patients younger than 64 years and carriers of -191CC genotype had higher risk (odds ratio/OR=9.6; 95% confidence interval/CI=0.8477 to 108.7214) for adenocarcinoma than the ones carrying -191CA or -191AA genotype. Conclusions: Patients younger than 64 years and carriers of -191CC genotype have significantly higher risk for adenocarcinoma than carriers of -191CA or -191AA genotype. Further studies on larger cohorts are necessary to evaluate -191C/A SNP as a potential biomarker.
Кључне речи:
smoking status / lung cancer / EGFR / adenocarcinomaИзвор:
Journal of Buon, 2018, 23, 2, 384-390Издавач:
- Balkan Union of Oncology (B.U.ON.)
Финансирање / пројекти:
- Ministry of Science and Technology, Republic of Srpska [19/6-020/961-123/14]
- Молекулске, биохемијске и имунолошке анализе у дијагностици тумора (RS-MESTD-Basic Research (BR or ON)-175056)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Elez-Burnjaković, Nikolina AU - Ugrin, Milena AU - Obradović, Jasmina AU - Miletić, Nataša AU - Racić, Maja AU - Kulić, Milan AU - Pavlović, Sonja AU - Jurisić, Vladimir PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1164 AB - Purpose: To analyze the frequencies of two single nucleotide polymorphisms (SNPs) of EGFR gene, -191C/A and 181946G/A, among lung cancer patients from the Republic of Srpska, Bosnia and Hercegovina, as well as to assess the association of SNP genotypes with the cancer type and other demographic characteristics of patients, particularly with the smoking status. Methods: This study enrolled 41 lung cancer patients from the territory of Republic Srpska, Bosnia and Herzegovina. Detection EGFR SNPs was performed using PCR-RFLP methodology. PCR was performed on 2720 Thermal Cycler (Applied Biosystems, United States). PCR, as well as RFLP products, were detected by gel electrophoresis. SPSS-17 soft-ware (SPSS, Inc.) was used for statistical analyses. Results: There was significantly more male than female smokers in our cohort (p=0.006). In addition, the proportion of smokers was higher among patients with adenocarcinoma in comparison to patients with other lung cancer types p=0.044). Adenocarcinoma was less common in patients older than 64 years (p=0.035). The wild type homozygous genotype of both SNPs was the most frequent genotype in all the tested demographic groups. Using dominant genetic model for -191C/A SNP, we observed statistically significant association of -191CC genotype and adenocarcinoma (p=0.043) in the subgroup of patients younger than 64 years. Namely, patients younger than 64 years and carriers of -191CC genotype had higher risk (odds ratio/OR=9.6; 95% confidence interval/CI=0.8477 to 108.7214) for adenocarcinoma than the ones carrying -191CA or -191AA genotype. Conclusions: Patients younger than 64 years and carriers of -191CC genotype have significantly higher risk for adenocarcinoma than carriers of -191CA or -191AA genotype. Further studies on larger cohorts are necessary to evaluate -191C/A SNP as a potential biomarker. PB - Balkan Union of Oncology (B.U.ON.) T2 - Journal of Buon T1 - Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina EP - 390 IS - 2 SP - 384 VL - 23 UR - https://hdl.handle.net/21.15107/rcub_imagine_1164 ER -
@article{ author = "Elez-Burnjaković, Nikolina and Ugrin, Milena and Obradović, Jasmina and Miletić, Nataša and Racić, Maja and Kulić, Milan and Pavlović, Sonja and Jurisić, Vladimir", year = "2018", abstract = "Purpose: To analyze the frequencies of two single nucleotide polymorphisms (SNPs) of EGFR gene, -191C/A and 181946G/A, among lung cancer patients from the Republic of Srpska, Bosnia and Hercegovina, as well as to assess the association of SNP genotypes with the cancer type and other demographic characteristics of patients, particularly with the smoking status. Methods: This study enrolled 41 lung cancer patients from the territory of Republic Srpska, Bosnia and Herzegovina. Detection EGFR SNPs was performed using PCR-RFLP methodology. PCR was performed on 2720 Thermal Cycler (Applied Biosystems, United States). PCR, as well as RFLP products, were detected by gel electrophoresis. SPSS-17 soft-ware (SPSS, Inc.) was used for statistical analyses. Results: There was significantly more male than female smokers in our cohort (p=0.006). In addition, the proportion of smokers was higher among patients with adenocarcinoma in comparison to patients with other lung cancer types p=0.044). Adenocarcinoma was less common in patients older than 64 years (p=0.035). The wild type homozygous genotype of both SNPs was the most frequent genotype in all the tested demographic groups. Using dominant genetic model for -191C/A SNP, we observed statistically significant association of -191CC genotype and adenocarcinoma (p=0.043) in the subgroup of patients younger than 64 years. Namely, patients younger than 64 years and carriers of -191CC genotype had higher risk (odds ratio/OR=9.6; 95% confidence interval/CI=0.8477 to 108.7214) for adenocarcinoma than the ones carrying -191CA or -191AA genotype. Conclusions: Patients younger than 64 years and carriers of -191CC genotype have significantly higher risk for adenocarcinoma than carriers of -191CA or -191AA genotype. Further studies on larger cohorts are necessary to evaluate -191C/A SNP as a potential biomarker.", publisher = "Balkan Union of Oncology (B.U.ON.)", journal = "Journal of Buon", title = "Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina", pages = "390-384", number = "2", volume = "23", url = "https://hdl.handle.net/21.15107/rcub_imagine_1164" }
Elez-Burnjaković, N., Ugrin, M., Obradović, J., Miletić, N., Racić, M., Kulić, M., Pavlović, S.,& Jurisić, V.. (2018). Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina. in Journal of Buon Balkan Union of Oncology (B.U.ON.)., 23(2), 384-390. https://hdl.handle.net/21.15107/rcub_imagine_1164
Elez-Burnjaković N, Ugrin M, Obradović J, Miletić N, Racić M, Kulić M, Pavlović S, Jurisić V. Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina. in Journal of Buon. 2018;23(2):384-390. https://hdl.handle.net/21.15107/rcub_imagine_1164 .
Elez-Burnjaković, Nikolina, Ugrin, Milena, Obradović, Jasmina, Miletić, Nataša, Racić, Maja, Kulić, Milan, Pavlović, Sonja, Jurisić, Vladimir, "Distribution of EGFR SNPs-191C/A and 181946G/A in patients with lung cancer depending on smoking status in the Republic of Srpska, Bosnia and Herzegovina" in Journal of Buon, 23, no. 2 (2018):384-390, https://hdl.handle.net/21.15107/rcub_imagine_1164 .