Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis
Аутори
Stanisavljević, SuzanaDinić, Miroslav
Jevtić, Bojan
Dedović, Neda
Momcilović, Miljana
Đokić, Jelena
Golić, Nataša
Mostarica-Stojković, Marija
Miljković, Đorđe
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site... of immunization. Upregulation of interferon-. and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis.
Кључне речи:
multiple sclerosis / gut-associated lymphoid tissue / gut microbiota / gut microbiota transfer / experimental autoimmune encephalomyelitis / antibioticsИзвор:
Frontiers in Immunology, 2018, 9Издавач:
- Frontiers Media Sa, Lausanne
Финансирање / пројекти:
- Ћелијски и молекулски механизми опоравка пацова од експерименталног аутоимунског енцефаломијелитиса (RS-MESTD-Basic Research (BR or ON)-173035)
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-MESTD-Basic Research (BR or ON)-173019)
- Имунопатогенетски и регулаторни механизми у аутоимунским болестима и хроничној инфламацији (RS-MESTD-Basic Research (BR or ON)-175038)
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.3389/fimmu.2018.00942
ISSN: 1664-3224
WoS: 000431197600001
Scopus: 2-s2.0-85046279847
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stanisavljević, Suzana AU - Dinić, Miroslav AU - Jevtić, Bojan AU - Dedović, Neda AU - Momcilović, Miljana AU - Đokić, Jelena AU - Golić, Nataša AU - Mostarica-Stojković, Marija AU - Miljković, Đorđe PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1179 AB - Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site of immunization. Upregulation of interferon-. and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Immunology T1 - Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis VL - 9 DO - 10.3389/fimmu.2018.00942 ER -
@article{ author = "Stanisavljević, Suzana and Dinić, Miroslav and Jevtić, Bojan and Dedović, Neda and Momcilović, Miljana and Đokić, Jelena and Golić, Nataša and Mostarica-Stojković, Marija and Miljković, Đorđe", year = "2018", abstract = "Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site of immunization. Upregulation of interferon-. and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Immunology", title = "Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis", volume = "9", doi = "10.3389/fimmu.2018.00942" }
Stanisavljević, S., Dinić, M., Jevtić, B., Dedović, N., Momcilović, M., Đokić, J., Golić, N., Mostarica-Stojković, M.,& Miljković, Đ.. (2018). Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis. in Frontiers in Immunology Frontiers Media Sa, Lausanne., 9. https://doi.org/10.3389/fimmu.2018.00942
Stanisavljević S, Dinić M, Jevtić B, Dedović N, Momcilović M, Đokić J, Golić N, Mostarica-Stojković M, Miljković Đ. Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis. in Frontiers in Immunology. 2018;9. doi:10.3389/fimmu.2018.00942 .
Stanisavljević, Suzana, Dinić, Miroslav, Jevtić, Bojan, Dedović, Neda, Momcilović, Miljana, Đokić, Jelena, Golić, Nataša, Mostarica-Stojković, Marija, Miljković, Đorđe, "Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis" in Frontiers in Immunology, 9 (2018), https://doi.org/10.3389/fimmu.2018.00942 . .