The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier
Autori
Vujicić, MilicaSaksida, Tamara
Despotović, Sanja
Soković Bajić, Svetlana
Lalić, Ivana
Koprivica, Ivan
Gajić, Dragica
Golić, Nataša
Tolinački, Maja
Stojanović, Ivana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-gamma in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the b...arrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function.
Izvor:
Scientific Reports, 2018, 8Izdavač:
- Nature Publishing Group, London
Finansiranje / projekti:
- Molekularni mehanizmi fiziološke i farmakološke kontrole inflamacije i kancera (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1038/s41598-018-24706-3
ISSN: 2045-2322
PubMed: 29679061
WoS: 000430537800014
Scopus: 2-s2.0-85045886334
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Vujicić, Milica AU - Saksida, Tamara AU - Despotović, Sanja AU - Soković Bajić, Svetlana AU - Lalić, Ivana AU - Koprivica, Ivan AU - Gajić, Dragica AU - Golić, Nataša AU - Tolinački, Maja AU - Stojanović, Ivana PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1182 AB - Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-gamma in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function. PB - Nature Publishing Group, London T2 - Scientific Reports T1 - The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier VL - 8 DO - 10.1038/s41598-018-24706-3 ER -
@article{ author = "Vujicić, Milica and Saksida, Tamara and Despotović, Sanja and Soković Bajić, Svetlana and Lalić, Ivana and Koprivica, Ivan and Gajić, Dragica and Golić, Nataša and Tolinački, Maja and Stojanović, Ivana", year = "2018", abstract = "Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-gamma in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function.", publisher = "Nature Publishing Group, London", journal = "Scientific Reports", title = "The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier", volume = "8", doi = "10.1038/s41598-018-24706-3" }
Vujicić, M., Saksida, T., Despotović, S., Soković Bajić, S., Lalić, I., Koprivica, I., Gajić, D., Golić, N., Tolinački, M.,& Stojanović, I.. (2018). The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier. in Scientific Reports Nature Publishing Group, London., 8. https://doi.org/10.1038/s41598-018-24706-3
Vujicić M, Saksida T, Despotović S, Soković Bajić S, Lalić I, Koprivica I, Gajić D, Golić N, Tolinački M, Stojanović I. The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier. in Scientific Reports. 2018;8. doi:10.1038/s41598-018-24706-3 .
Vujicić, Milica, Saksida, Tamara, Despotović, Sanja, Soković Bajić, Svetlana, Lalić, Ivana, Koprivica, Ivan, Gajić, Dragica, Golić, Nataša, Tolinački, Maja, Stojanović, Ivana, "The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier" in Scientific Reports, 8 (2018), https://doi.org/10.1038/s41598-018-24706-3 . .