Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations
Abstract
After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity The genetic determina...nts identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results.
Keywords:
starvation / regrowth / oxidative stress / liquid holding / genome integritySource:
Frontiers in Microbiology, 2018, 9Publisher:
- Frontiers Media Sa, Lausanne
Funding / projects:
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- Collaborative Research Program International Centre for Genetic Engineering and Biotechnology (CRP-ICGEB) Research Grant [YUG 14-01]
DOI: 10.3389/fmicb.2018.00990
ISSN: 1664-302X
WoS: 000432383500001
Scopus: 2-s2.0-85047156319
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Milisavljević, Mira AU - Petković, Jelena AU - Samardžić, Jelena AU - Kojić, Milorad PY - 2018 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1186 AB - After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity The genetic determinants identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations VL - 9 DO - 10.3389/fmicb.2018.00990 ER -
@article{ author = "Milisavljević, Mira and Petković, Jelena and Samardžić, Jelena and Kojić, Milorad", year = "2018", abstract = "After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity The genetic determinants identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations", volume = "9", doi = "10.3389/fmicb.2018.00990" }
Milisavljević, M., Petković, J., Samardžić, J.,& Kojić, M.. (2018). Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 9. https://doi.org/10.3389/fmicb.2018.00990
Milisavljević M, Petković J, Samardžić J, Kojić M. Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations. in Frontiers in Microbiology. 2018;9. doi:10.3389/fmicb.2018.00990 .
Milisavljević, Mira, Petković, Jelena, Samardžić, Jelena, Kojić, Milorad, "Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations" in Frontiers in Microbiology, 9 (2018), https://doi.org/10.3389/fmicb.2018.00990 . .