Radiation effects on early phase of NT2/D1 neural differentiation in vitro
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Stanisavljević Ninković, DanijelaPopović, Jelena
Petrović, Isidora
Davidović, Slobodan
Atkinson, Michael J.
Anastasov, Nataša
Stevanović, Milena
Article (Published version)
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Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show t...hat 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
Keywords:
radiation / NT2 / neural differentiation / miRNAs / markers of pluripotency / D1 cell lineSource:
International Journal of Radiation Biology, 2019, 95, 12, 1627-1639Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Studying signal transduction pathways and epigenetic mechanisms that control human SOX genes expression: further insight into their roles in cell fate determination and differentiation (RS-MESTD-Basic Research (BR or ON)-173051)
- Serbian Academy of Sciences and Arts [F 24]
- EURATOM Fission and European Commission 7th Framework Programme, DarkRisk [323216]
- IBRO/PERC InEurope Short Stay Grants
Note:
- Peer-reviewed manuscript: https://imagine.imgge.bg.ac.rs/handle/123456789/1629
Related info:
DOI: 10.1080/09553002.2019.1665207
ISSN: 0955-3002
PubMed: 31509479
WoS: 000486834100001
Scopus: 2-s2.0-85073825834
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stanisavljević Ninković, Danijela AU - Popović, Jelena AU - Petrović, Isidora AU - Davidović, Slobodan AU - Atkinson, Michael J. AU - Anastasov, Nataša AU - Stevanović, Milena PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1206 AB - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies. PB - Taylor & Francis Ltd, Abingdon T2 - International Journal of Radiation Biology T1 - Radiation effects on early phase of NT2/D1 neural differentiation in vitro EP - 1639 IS - 12 SP - 1627 VL - 95 DO - 10.1080/09553002.2019.1665207 ER -
@article{ author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena", year = "2019", abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "International Journal of Radiation Biology", title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro", pages = "1639-1627", number = "12", volume = "95", doi = "10.1080/09553002.2019.1665207" }
Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639. https://doi.org/10.1080/09553002.2019.1665207
Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639. doi:10.1080/09553002.2019.1665207 .
Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639, https://doi.org/10.1080/09553002.2019.1665207 . .