Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)
Нема приказа
Аутори
Mojicević, MarijaD'Agostino, Paul M.
Nikodinović-Runić, Jasmina
Vasiljević, Branka
Gulder, Tobias A. M.
Vojnović, Sandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability ...to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.
Кључне речи:
Streptomyces / Soil isolates / Screening / Ethno-medicinal plants / Antifungal activityИзвор:
International Microbiology, 2019, 22, 3, 343-353Издавач:
- Springer, New York
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
- DAAD (Deutscher Akademischer Austauschdienst, Bilateral Project with Republic of Serbia)
DOI: 10.1007/s10123-019-00054-8
ISSN: 1139-6709
PubMed: 30810997
WoS: 000483620200006
Scopus: 2-s2.0-85071610847
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Mojicević, Marija AU - D'Agostino, Paul M. AU - Nikodinović-Runić, Jasmina AU - Vasiljević, Branka AU - Gulder, Tobias A. M. AU - Vojnović, Sandra PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1212 AB - The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds. PB - Springer, New York T2 - International Microbiology T1 - Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle) EP - 353 IS - 3 SP - 343 VL - 22 DO - 10.1007/s10123-019-00054-8 ER -
@article{ author = "Mojicević, Marija and D'Agostino, Paul M. and Nikodinović-Runić, Jasmina and Vasiljević, Branka and Gulder, Tobias A. M. and Vojnović, Sandra", year = "2019", abstract = "The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 mu g/mL. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. Our results proved that rhizosphere soil of ethno-medicinal plants is a prolific source of streptomycetes, producers of potentially new antifungal compounds.", publisher = "Springer, New York", journal = "International Microbiology", title = "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)", pages = "353-343", number = "3", volume = "22", doi = "10.1007/s10123-019-00054-8" }
Mojicević, M., D'Agostino, P. M., Nikodinović-Runić, J., Vasiljević, B., Gulder, T. A. M.,& Vojnović, S.. (2019). Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology Springer, New York., 22(3), 343-353. https://doi.org/10.1007/s10123-019-00054-8
Mojicević M, D'Agostino PM, Nikodinović-Runić J, Vasiljević B, Gulder TAM, Vojnović S. Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle). in International Microbiology. 2019;22(3):343-353. doi:10.1007/s10123-019-00054-8 .
Mojicević, Marija, D'Agostino, Paul M., Nikodinović-Runić, Jasmina, Vasiljević, Branka, Gulder, Tobias A. M., Vojnović, Sandra, "Antifungal potential of bacterial rhizosphere isolates associated with three ethno-medicinal plants (poppy, chamomile, and nettle)" in International Microbiology, 22, no. 3 (2019):343-353, https://doi.org/10.1007/s10123-019-00054-8 . .