Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells
Аутори
Dragičević, SandraKovacević, Draginja
Divac Rankov, Aleksandra
Nikolić, Aleksandra
Radojković, Dragica
Radović, Svetlana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Tussilago farfara (coltsfoot) and Verbascum thapsus (mullein) have been used as folk remedies for treating respiratory disorders. The aim of this study was to test the toxicity of the water extracts of T. farfara and V. thapsus in vivo in zebrafish and in vitro in BEAS 2B epithelial bronchial cells. To the best of our knowledge, this is the first study to investigate the antioxidative properties of T. farfara and V. thapsus extracts in cell culture. Our results show that the T. farfara leaf extract does not produce toxic effects on zebrafish embryos or BEAS 2B cells, and that it has a protective effect in BEAS 2B after induction of oxidative stress. The water extract from V. thapsus displayed pronounced toxic effects on zebrafish embryos and BEAS 2B cells and did not exhibit a significant antioxidative effect on BEAS 2B cells exposed to oxidative stress. Our results suggest that the use of T. farfara water leaf extract is potentially safe and effective in treating respiratory disorders..., whereas the use of V. thapsus needs further investigation.
Кључне речи:
zebrafish / Verbascum thapsus (mullein) / Tussilago farfara (coltsfoot) / BEAS 2B cells / asthma treatmentИзвор:
Archives of Biological Sciences, 2019, 71, 3, 409-416Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.2298/ABS181213022D
ISSN: 0354-4664
WoS: 000491998600003
Scopus: 2-s2.0-85076493895
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Dragičević, Sandra AU - Kovacević, Draginja AU - Divac Rankov, Aleksandra AU - Nikolić, Aleksandra AU - Radojković, Dragica AU - Radović, Svetlana PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1233 AB - Tussilago farfara (coltsfoot) and Verbascum thapsus (mullein) have been used as folk remedies for treating respiratory disorders. The aim of this study was to test the toxicity of the water extracts of T. farfara and V. thapsus in vivo in zebrafish and in vitro in BEAS 2B epithelial bronchial cells. To the best of our knowledge, this is the first study to investigate the antioxidative properties of T. farfara and V. thapsus extracts in cell culture. Our results show that the T. farfara leaf extract does not produce toxic effects on zebrafish embryos or BEAS 2B cells, and that it has a protective effect in BEAS 2B after induction of oxidative stress. The water extract from V. thapsus displayed pronounced toxic effects on zebrafish embryos and BEAS 2B cells and did not exhibit a significant antioxidative effect on BEAS 2B cells exposed to oxidative stress. Our results suggest that the use of T. farfara water leaf extract is potentially safe and effective in treating respiratory disorders, whereas the use of V. thapsus needs further investigation. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells EP - 416 IS - 3 SP - 409 VL - 71 DO - 10.2298/ABS181213022D ER -
@article{ author = "Dragičević, Sandra and Kovacević, Draginja and Divac Rankov, Aleksandra and Nikolić, Aleksandra and Radojković, Dragica and Radović, Svetlana", year = "2019", abstract = "Tussilago farfara (coltsfoot) and Verbascum thapsus (mullein) have been used as folk remedies for treating respiratory disorders. The aim of this study was to test the toxicity of the water extracts of T. farfara and V. thapsus in vivo in zebrafish and in vitro in BEAS 2B epithelial bronchial cells. To the best of our knowledge, this is the first study to investigate the antioxidative properties of T. farfara and V. thapsus extracts in cell culture. Our results show that the T. farfara leaf extract does not produce toxic effects on zebrafish embryos or BEAS 2B cells, and that it has a protective effect in BEAS 2B after induction of oxidative stress. The water extract from V. thapsus displayed pronounced toxic effects on zebrafish embryos and BEAS 2B cells and did not exhibit a significant antioxidative effect on BEAS 2B cells exposed to oxidative stress. Our results suggest that the use of T. farfara water leaf extract is potentially safe and effective in treating respiratory disorders, whereas the use of V. thapsus needs further investigation.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells", pages = "416-409", number = "3", volume = "71", doi = "10.2298/ABS181213022D" }
Dragičević, S., Kovacević, D., Divac Rankov, A., Nikolić, A., Radojković, D.,& Radović, S.. (2019). Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 71(3), 409-416. https://doi.org/10.2298/ABS181213022D
Dragičević S, Kovacević D, Divac Rankov A, Nikolić A, Radojković D, Radović S. Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells. in Archives of Biological Sciences. 2019;71(3):409-416. doi:10.2298/ABS181213022D .
Dragičević, Sandra, Kovacević, Draginja, Divac Rankov, Aleksandra, Nikolić, Aleksandra, Radojković, Dragica, Radović, Svetlana, "Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells" in Archives of Biological Sciences, 71, no. 3 (2019):409-416, https://doi.org/10.2298/ABS181213022D . .