Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia)
Nema prikaza
Autori
Kerkez-Janković, IvonaNonić, Marina
Devetaković, Jovana
Ivetić, Vladan
Sijacić-Nikolić, Mirjana
Aleksić, Jelena M.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Selectively neutral nuclear microsatellites (SSRs) are available for Fagus sp., and have been used in F. sylvatica, an ecologically and economically most important European Fagus species. We provide a comprehensive technical overview of available Fagus sp. SSRs (185 loci), carry out meta-analysis of loci used in F. sylvatica (62 loci employed in 62 surveys), and validate a set of 16 loci in 45 individuals of this species from the central Balkans (Serbia). Erroneous usage of marker's names/authors is rather frequent, and loci successfully used in a number of studies are characterized by other authors by high prevalence of null alleles and even multilocus amplification products. Frequent occurrence of null alleles at FS4-46, used in 26 surveys to date, most likely indicates a failure to record multiple alleles at this locus. Twelve loci are reliable/informative in F. sylvatica from the Balkans (5-18 alleles/locus, H-E ranging from 0.523 to 0.850), while four loci are characterized by hig...h prevalence of null alleles (sfc0161 and sfc1063) and multilocus amplification products (FS4-46 and Fagsyl_007038). Our findings are important for future population genetics and studies on adaptation of F. sylvatica to its environment, because the latter rely on both selectively neutral and adaptive markers under selection.
Ključne reči:
simple sequence repeats (SSRs) / null alleles / multilocus amplification products / genotyping / common beech / Balkan PeninsulaIzvor:
Scandinavian Journal of Forest Research, 2019, 34, 7, 545-556Izdavač:
- Taylor & Francis As, Oslo
Finansiranje / projekti:
- Ministry of Agriculture, Forestry and Water Management of the Republic of Serbia - Forest Directorate
- Biodiverzitet biljnog sveta Srbije i Balkanskog poluostrva - procena, održivo korišćenje i zaštita (RS-MESTD-Basic Research (BR or ON)-173030)
- Šumski zasadi u funkciji povećanja pošumljenosti Srbije (RS-MESTD-Technological Development (TD or TR)-31041)
DOI: 10.1080/02827581.2019.1623305
ISSN: 0282-7581
WoS: 000471514600001
Scopus: 2-s2.0-85067547643
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kerkez-Janković, Ivona AU - Nonić, Marina AU - Devetaković, Jovana AU - Ivetić, Vladan AU - Sijacić-Nikolić, Mirjana AU - Aleksić, Jelena M. PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1239 AB - Selectively neutral nuclear microsatellites (SSRs) are available for Fagus sp., and have been used in F. sylvatica, an ecologically and economically most important European Fagus species. We provide a comprehensive technical overview of available Fagus sp. SSRs (185 loci), carry out meta-analysis of loci used in F. sylvatica (62 loci employed in 62 surveys), and validate a set of 16 loci in 45 individuals of this species from the central Balkans (Serbia). Erroneous usage of marker's names/authors is rather frequent, and loci successfully used in a number of studies are characterized by other authors by high prevalence of null alleles and even multilocus amplification products. Frequent occurrence of null alleles at FS4-46, used in 26 surveys to date, most likely indicates a failure to record multiple alleles at this locus. Twelve loci are reliable/informative in F. sylvatica from the Balkans (5-18 alleles/locus, H-E ranging from 0.523 to 0.850), while four loci are characterized by high prevalence of null alleles (sfc0161 and sfc1063) and multilocus amplification products (FS4-46 and Fagsyl_007038). Our findings are important for future population genetics and studies on adaptation of F. sylvatica to its environment, because the latter rely on both selectively neutral and adaptive markers under selection. PB - Taylor & Francis As, Oslo T2 - Scandinavian Journal of Forest Research T1 - Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia) EP - 556 IS - 7 SP - 545 VL - 34 DO - 10.1080/02827581.2019.1623305 ER -
@article{ author = "Kerkez-Janković, Ivona and Nonić, Marina and Devetaković, Jovana and Ivetić, Vladan and Sijacić-Nikolić, Mirjana and Aleksić, Jelena M.", year = "2019", abstract = "Selectively neutral nuclear microsatellites (SSRs) are available for Fagus sp., and have been used in F. sylvatica, an ecologically and economically most important European Fagus species. We provide a comprehensive technical overview of available Fagus sp. SSRs (185 loci), carry out meta-analysis of loci used in F. sylvatica (62 loci employed in 62 surveys), and validate a set of 16 loci in 45 individuals of this species from the central Balkans (Serbia). Erroneous usage of marker's names/authors is rather frequent, and loci successfully used in a number of studies are characterized by other authors by high prevalence of null alleles and even multilocus amplification products. Frequent occurrence of null alleles at FS4-46, used in 26 surveys to date, most likely indicates a failure to record multiple alleles at this locus. Twelve loci are reliable/informative in F. sylvatica from the Balkans (5-18 alleles/locus, H-E ranging from 0.523 to 0.850), while four loci are characterized by high prevalence of null alleles (sfc0161 and sfc1063) and multilocus amplification products (FS4-46 and Fagsyl_007038). Our findings are important for future population genetics and studies on adaptation of F. sylvatica to its environment, because the latter rely on both selectively neutral and adaptive markers under selection.", publisher = "Taylor & Francis As, Oslo", journal = "Scandinavian Journal of Forest Research", title = "Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia)", pages = "556-545", number = "7", volume = "34", doi = "10.1080/02827581.2019.1623305" }
Kerkez-Janković, I., Nonić, M., Devetaković, J., Ivetić, V., Sijacić-Nikolić, M.,& Aleksić, J. M.. (2019). Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia). in Scandinavian Journal of Forest Research Taylor & Francis As, Oslo., 34(7), 545-556. https://doi.org/10.1080/02827581.2019.1623305
Kerkez-Janković I, Nonić M, Devetaković J, Ivetić V, Sijacić-Nikolić M, Aleksić JM. Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia). in Scandinavian Journal of Forest Research. 2019;34(7):545-556. doi:10.1080/02827581.2019.1623305 .
Kerkez-Janković, Ivona, Nonić, Marina, Devetaković, Jovana, Ivetić, Vladan, Sijacić-Nikolić, Mirjana, Aleksić, Jelena M., "Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia)" in Scandinavian Journal of Forest Research, 34, no. 7 (2019):545-556, https://doi.org/10.1080/02827581.2019.1623305 . .