Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin
2019
Аутори
Pavić, AleksandarMitić-Culafić, Dragana
Nebojša, Jasnic
Nikolić, Biljana
Simin, Nataša
Vasiljević, Branka
Knežević-Vukčević, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and ...no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used anti-angiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments.
Кључне речи:
Zebrafish embryos / Doxorubicin / Cardio- and myeloprotection / Anticancer activity / Anti-angiogenesis / Allium extractsИзвор:
Biomedicine & Pharmacotherapy, 2019, 109, 2482-2491Издавач:
- Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
Финансирање / пројекти:
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
- Биолошки активни природни производи као потенцијални извори нових лекова и дијететских суплемената (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.1016/j.biopha.2018.11.106
ISSN: 0753-3322
PubMed: 30551509
WoS: 000452539100271
Scopus: 2-s2.0-85057496768
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Pavić, Aleksandar AU - Mitić-Culafić, Dragana AU - Nebojša, Jasnic AU - Nikolić, Biljana AU - Simin, Nataša AU - Vasiljević, Branka AU - Knežević-Vukčević, Jelena PY - 2019 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1240 AB - The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used anti-angiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments. PB - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux T2 - Biomedicine & Pharmacotherapy T1 - Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin EP - 2491 SP - 2482 VL - 109 DO - 10.1016/j.biopha.2018.11.106 ER -
@article{ author = "Pavić, Aleksandar and Mitić-Culafić, Dragana and Nebojša, Jasnic and Nikolić, Biljana and Simin, Nataša and Vasiljević, Branka and Knežević-Vukčević, Jelena", year = "2019", abstract = "The objective of this study was to evaluate potential of two chemically characterized edible wild onion species, Allium flavum and Allium carinatum, to reduce side effects of cytostatic doxorubicin (Dox). Since Dox application is mainly limited due to its high cardiotoxicity, while there are no approved cardioprotective agents for the prevention of Dox adverse effects, new co-treatments are urgently needed. Here, we showed that methanol extracts expressed high antioxidant activity and synergistically increased Dox anticancer activity against human hepatoma (HepG2) and lung carcinoma (A549) cells, while protected normal human fibroblasts (MRC-5) from Dox cytotoxicity. Analysis of the antioxidative enzymes level (catalase and superoxide dismutases) showed that the catalase level was differently altered in cancer cells compared to normal cells upon applied treatments. In vivo toxicity evaluation in the zebrafish model revealed significantly lower toxicity of extracts compared to Dox, and no teratogenic effects at applied doses. We found that extracts successfully rescued the Dox-treated embryos of life-threating cardiomyopathy, while at the same time reduced developmental toxicity and neutropenia. Further analysis demonstrated that extracts had higher anti-angiogenic activity than sunitinib or auranofin, clinically used anti-angiogenic drugs. In addition, angiogenesis was markedly more suppressed in Dox-extract cotreatments than upon single treatments.", publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux", journal = "Biomedicine & Pharmacotherapy", title = "Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin", pages = "2491-2482", volume = "109", doi = "10.1016/j.biopha.2018.11.106" }
Pavić, A., Mitić-Culafić, D., Nebojša, J., Nikolić, B., Simin, N., Vasiljević, B.,& Knežević-Vukčević, J.. (2019). Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin. in Biomedicine & Pharmacotherapy Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 109, 2482-2491. https://doi.org/10.1016/j.biopha.2018.11.106
Pavić A, Mitić-Culafić D, Nebojša J, Nikolić B, Simin N, Vasiljević B, Knežević-Vukčević J. Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin. in Biomedicine & Pharmacotherapy. 2019;109:2482-2491. doi:10.1016/j.biopha.2018.11.106 .
Pavić, Aleksandar, Mitić-Culafić, Dragana, Nebojša, Jasnic, Nikolić, Biljana, Simin, Nataša, Vasiljević, Branka, Knežević-Vukčević, Jelena, "Wild edible onions - Allium flavum and Allium carinatum - successfully prevent adverse effects of chemotherapeutic drug doxorubicin" in Biomedicine & Pharmacotherapy, 109 (2019):2482-2491, https://doi.org/10.1016/j.biopha.2018.11.106 . .